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Bioengineering
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Bioanalysis Systems: Materials, Methods, Designs and Applications
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Bioanalysis Systems: Materials, Methods, Designs and Applications

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biochemical Engineering".

Deadline for manuscript submissions: closed (29 February 2024) | Viewed by 7176

Special Issue Editor

Dr. Zongjie Wang

E-Mail Website
Guest Editor
Faculty of Pharmacy, Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada
Interests: biofabrication; biomicrofluidics; biointerface; cell therapy; precision medicine; mechanobiology; stem cells; regenerative medicine
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The last two years represent a period unlike anything that has been experienced in the modern era. The pandemic and our response have spotlighted areas of phenomenal scientific progress, including those advancements made in bioanalysis systems. We can now appreciate the importance of decentralized, on-demand rapid testing as well as the accompanying telemedicine and virtual care.

As a result, the future of sensing science is bright and is rapidly emerging. Tremendous amounts of new materials, fabrication methods, and design operations have contributed to the development of next-generation bioanalysis systems that are low-cost, sensitive, rapid, scalable, and straightforward to use.

In this Special Issue, we aim to collect articles shaping the future of bioanalysis systems. We welcome original submissions regarding the novel materials, methods, designs, and applications of bioanalysis systems. We also seek insightful reviews, perspectives, and comments to comprehensively understand the recent advances and remaining challenges in the field.

Potential topics include but are not limited to the following:

  • Micro/nanofluidics;
  • Portable bioanalytic devices;
  • Wearable bioanalytic devices;
  • Functional materials for bioanalysis;
  • The advanced fabrication and manufacturing of bioanalysis systems;
  • Novel methods and principles of analysis;
  • Applications of bioanalysis systems.

Dr. Zongjie (Daniel) Wang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • material chemistry
  • analytical chemistry
  • biophysics
  • nanotechnology
  • microfluidics
  • diagnosis
  • theranostics

Published Papers (5 papers)

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Research

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12 pages, 5475 KiB  
Article
Delamination Strength and Elastin Interlaminar Fibers Decrease with the Development of Aortic Dissection in Model Rats
by Genki Kurihara, Yoshihiro Ujihara, Masanori Nakamura and Shukei Sugita
Bioengineering 2023, 10(11), 1292; https://doi.org/10.3390/bioengineering10111292 - 08 Nov 2023
Viewed by 1388
Abstract
Aortic dissection (AD) is a life-threatening tear of the vascular tissue with creation of a false lumen. To explore the mechanism underlying this tissue tear, this study investigated the delamination strength of AD model rats and the histological composition of the aorta at [...] Read more.
Aortic dissection (AD) is a life-threatening tear of the vascular tissue with creation of a false lumen. To explore the mechanism underlying this tissue tear, this study investigated the delamination strength of AD model rats and the histological composition of the aorta at various stages of AD development. SD rats were administrated beta-amino propionitrile for 0 (Control), 3 (Pre-dissection), and 6 (Dissection) weeks. The thoracic aorta was harvested at 10–11 weeks of age. The Dissection group exclusively showed AD at the ascending aorta. The delamination strength, a force that separates the aorta in the radial direction, of the descending aorta decreased significantly in the order of the Control, Pre-dissection, and Dissection groups. A quantitative histological analysis of the aortic tissue demonstrated that, compared with the Control group, the area fraction of collagen was significantly higher in the Pre-dissection and Dissection groups and that of elastin was significantly lower in the Dissection group. The area fraction of the elastin fibers between the elastic laminas (interlaminar fibers) was significantly decreased in the order of the Control, Pre-dissection, and Dissection groups. Histological changes of the aortic tissue, perhaps a reduction in interlaminar fibers mainly aligned in the radial direction, decreased delamination strength, thereby causing AD. Full article
(This article belongs to the Special Issue Bioanalysis Systems: Materials, Methods, Designs and Applications)
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15 pages, 5610 KiB  
Article
Study of the Relationship between Pulmonary Artery Pressure and Heart Valve Vibration Sound Based on Mock Loop
by Jiachen Mi, Zehang Zhao, Hongkai Wang and Hong Tang
Bioengineering 2023, 10(8), 985; https://doi.org/10.3390/bioengineering10080985 - 20 Aug 2023
Cited by 1 | Viewed by 1023
Abstract
The vibration of the heart valves’ closure is an important component of the heart sound and contains important information about the mechanical activity of a heart. Stenosis of the distal pulmonary artery can lead to pulmonary hypertension (PH). Therefore, in this paper, the [...] Read more.
The vibration of the heart valves’ closure is an important component of the heart sound and contains important information about the mechanical activity of a heart. Stenosis of the distal pulmonary artery can lead to pulmonary hypertension (PH). Therefore, in this paper, the relationship between the vibration sound of heart valves and the pulmonary artery blood pressure was investigated to contribute to the noninvasive detection of PH. In this paper, a lumped parameter circuit platform of pulmonary circulation was first set to guide the establishment of a mock loop of circulation. By adjusting the distal vascular resistance of the pulmonary artery, six different pulmonary arterial pressure states were achieved. In the experiment, pulmonary artery blood pressure, right ventricular blood pressure, and the vibration sound of the pulmonary valve and tricuspid valve were measured synchronously. Features of the time domain and frequency domain of two valves’ vibration sound were extracted. By conducting a significance analysis of the inter-group features, it was found that the amplitude, energy and frequency features of vibration sounds changed significantly. Finally, the continuously varied pulmonary arterial blood pressure and valves’ vibration sound were obtained by continuously adjusting the resistance of the distal pulmonary artery. A backward propagation neural network and deep learning model were used, respectively, to estimate the features of pulmonary arterial blood pressure, pulmonary artery systolic blood pressure, the maximum rising rate of pulmonary artery blood pressure and the maximum falling rate of pulmonary artery blood pressure by the vibration sound of the pulmonary and tricuspid valves. The results showed that the pulmonary artery pressure parameters can be well estimated by valve vibration sounds. Full article
(This article belongs to the Special Issue Bioanalysis Systems: Materials, Methods, Designs and Applications)
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11 pages, 2163 KiB  
Article
Comparative Analysis of NanoLuc Luciferase and Alkaline Phosphatase Luminescence Reporter Systems for Phage-Based Detection of Bacteria
by Shalini Wijeratne, Arindam Bakshi and Joey Talbert
Bioengineering 2022, 9(9), 479; https://doi.org/10.3390/bioengineering9090479 - 16 Sep 2022
Cited by 1 | Viewed by 1712
Abstract
Reporter phage assays are a promising alternative to culture-based assays for rapidly detecting viable bacteria. The reporter systems used in phage-based detection are typically enzymes and their corresponding substrates that provide a signal following infection and expression. While several reporter systems have been [...] Read more.
Reporter phage assays are a promising alternative to culture-based assays for rapidly detecting viable bacteria. The reporter systems used in phage-based detection are typically enzymes and their corresponding substrates that provide a signal following infection and expression. While several reporter systems have been developed, comparing reporter systems based on reported bacteria detection limits from literature can be challenging due to factors other than the reporter system that influence detection capabilities. To advance the development of phage-based assays, a systematic comparison and understanding of the components are necessary. The objective of this study was to directly compare two common enzyme-mediated luminescence reporter systems, NanoLuc/Nano-Glo and alkaline phosphatase (ALP*)/DynaLight, for phage-based detection of bacteria. The detection limits of the purified enzymes were determined, as well as the expression levels and bacteria detection capabilities following engineering of the coding genes into T7 phage and infection of E. coli BL21. When comparing the sensitivity of the purified enzymes, NLuc/Nano-Glo enzyme/substrate system demonstrated a lower detection limit than ALP*/DynaLight. In addition, the expression of the NLuc reporter following phage infection of E. coli was greater than ALP*. The lower detection limit combined with the higher expression resulted in a greater than 100-fold increase in sensitivity for the NLuc/Nano-Glo® reporter system compared to ALP*/DynaLight when used for the detection of E. coli in a model system. These findings provide a comparative analysis of two common reporter systems used for phage-based detection of bacteria and a foundational understanding of these systems for engineering future reporter phage assays. Full article
(This article belongs to the Special Issue Bioanalysis Systems: Materials, Methods, Designs and Applications)
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31 pages, 6645 KiB  
Review
Recent Advances in Wearable Healthcare Devices: From Material to Application
by Xiao Luo, Handong Tan and Weijia Wen
Bioengineering 2024, 11(4), 358; https://doi.org/10.3390/bioengineering11040358 - 06 Apr 2024
Viewed by 603
Abstract
In recent years, the proliferation of wearable healthcare devices has marked a revolutionary shift in the personal health monitoring and management paradigm. These devices, ranging from fitness trackers to advanced biosensors, have not only made healthcare more accessible, but have also transformed the [...] Read more.
In recent years, the proliferation of wearable healthcare devices has marked a revolutionary shift in the personal health monitoring and management paradigm. These devices, ranging from fitness trackers to advanced biosensors, have not only made healthcare more accessible, but have also transformed the way individuals engage with their health data. By continuously monitoring health signs, from physical-based to biochemical-based such as heart rate and blood glucose levels, wearable technology offers insights into human health, enabling a proactive rather than a reactive approach to healthcare. This shift towards personalized health monitoring empowers individuals with the knowledge and tools to make informed decisions about their lifestyle and medical care, potentially leading to the earlier detection of health issues and more tailored treatment plans. This review presents the fabrication methods of flexible wearable healthcare devices and their applications in medical care. The potential challenges and future prospectives are also discussed. Full article
(This article belongs to the Special Issue Bioanalysis Systems: Materials, Methods, Designs and Applications)
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16 pages, 3143 KiB  
Review
Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine
by Zongjie Wang
Bioengineering 2023, 10(7), 857; https://doi.org/10.3390/bioengineering10070857 - 19 Jul 2023
Cited by 2 | Viewed by 1673
Abstract
Stem cells hold promise in regenerative medicine due to their ability to proliferate and differentiate into various cell types. However, their self-renewal and multipotency also raise concerns about their tumorigenicity during and post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived [...] Read more.
Stem cells hold promise in regenerative medicine due to their ability to proliferate and differentiate into various cell types. However, their self-renewal and multipotency also raise concerns about their tumorigenicity during and post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived tumors in animal models and clinical administrations. Therefore, the assessment of tumorigenicity is crucial in evaluating the safety of stem cell-derived therapeutic products. Ideally, the assessment needs to be performed rapidly, sensitively, cost-effectively, and scalable. This article reviews various approaches for assessing tumorigenicity, including animal models, soft agar culture, PCR, flow cytometry, and microfluidics. Each method has its advantages and limitations. The selection of the assay depends on the specific needs of the study and the stage of development of the stem cell-derived therapeutic product. Combining multiple assays may provide a more comprehensive evaluation of tumorigenicity. Future developments should focus on the optimization and standardization of microfluidics-based methods, as well as the integration of multiple assays into a single platform for efficient and comprehensive evaluation of tumorigenicity. Full article
(This article belongs to the Special Issue Bioanalysis Systems: Materials, Methods, Designs and Applications)
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