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16 pages, 3749 KiB

Open AccessArticle

Nitric Oxide Induces Autophagy in Triticum aestivum Roots

by Farida Minibayeva, Anastasia Mazina, Natalia Gazizova, Svetlana Dmitrieva, Anastasia Ponomareva and Daniya Rakhmatullina

Antioxidants 2023, 12(9), 1655; https://doi.org/10.3390/antiox12091655 - 22 Aug 2023

Cited by 1 | Viewed by 860

Abstract

Autophagy is a highly conserved process that degrades damaged macromolecules and organelles. Unlike animals, only scant information is available regarding nitric oxide (NO)-induced autophagy in plants. Such lack of information prompted us to study the roles of the NO donors’ nitrate, nitrite, and [...] Read more.

Autophagy is a highly conserved process that degrades damaged macromolecules and organelles. Unlike animals, only scant information is available regarding nitric oxide (NO)-induced autophagy in plants. Such lack of information prompted us to study the roles of the NO donors’ nitrate, nitrite, and sodium nitroprusside in this catabolic process in wheat roots. Furthermore, spermine, a polyamine that is found in all eukaryotic cells, was also tested as a physiological NO donor. Here, we show that in wheat roots, NO donors and spermine can trigger autophagy, with NO and reactive oxygen species (ROS) playing signaling roles based on the visualization of autophagosomes, analyses of the levels of NO, ROS, mitochondrial activity, and the expression of autophagic (ATG) genes. Treatment with nitrite and nitroprusside causes an energy deficit, a typical prerequisite of autophagy, which is indicated by a fall in mitochondrial potential, and the activity of mitochondrial complexes. On the contrary, spermine sustains energy metabolism by upregulating the activity of appropriate genes, including those that encode glyceraldehyde 3-phosphate dehydrogenase GAPDH and SNF1-related protein kinase 1 SnRK1. Taken together, our data suggest that one of the key roles for NO in plants may be to trigger autophagy via diverse mechanisms, thus facilitating the removal of oxidized and damaged cellular constituencies. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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12 pages, 1916 KiB

Open AccessCommunication

Differential Responses of Antioxidant Enzymes and Lignin Metabolism in Susceptible and Resistant Sweetpotato Cultivars during Root-Knot Nematode Infection

by Jung-Wook Yang, Sul-U Park, Hyeong-Un Lee, Ki Jung Nam, Kang-Lok Lee, Jeung Joo Lee, Ju Hwan Kim, Sang-Soo Kwak, Ho Soo Kim and Yun-Hee Kim

Antioxidants 2023, 12(6), 1164; https://doi.org/10.3390/antiox12061164 - 27 May 2023

Cited by 5 | Viewed by 1313

Abstract

Root-knot nematodes (RKN) cause significant damage to sweetpotato plants and cause significant losses in yield and quality. Reactive oxygen species (ROS) play an important role in plant defenses, with levels of ROS-detoxifying antioxidant enzymes tightly regulated during pathogen infection. In this study, ROS [...] Read more.

Root-knot nematodes (RKN) cause significant damage to sweetpotato plants and cause significant losses in yield and quality. Reactive oxygen species (ROS) play an important role in plant defenses, with levels of ROS-detoxifying antioxidant enzymes tightly regulated during pathogen infection. In this study, ROS metabolism was examined in three RKN-resistant and three RKN-susceptible sweetpotato cultivars. The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were assessed, as was lignin-related metabolism. In RKN-infected roots, both resistant and susceptible cultivars increased SOD activity to produce higher levels of hydrogen peroxide (H₂O₂). However, H₂O₂ removal by CAT activity differed between cultivars, with susceptible cultivars having higher CAT activity and lower overall H₂O₂ levels. In addition, the expression of phenylpropanoid-related phenylalanine ammonia-lyase and cinnamyl alcohol dehydrogenase genes, which encode enzymes involved in lignin metabolism, were higher in resistant cultivars, as were total phenolic and lignin contents. Enzyme activities and H₂O₂ levels were examined during the early (7 days) and late (28 days) phases of infection in representative susceptible and resistant cultivars, revealing contrasting changes in ROS levels and antioxidant responses in the different stages of infection. This study suggests that differences in antioxidant enzyme activities and ROS regulation in resistant and susceptible cultivars might explain reduced RKN infection in resistant cultivars, resulting in smaller RKN populations and overall higher resistance to infection and infestation by RKNs. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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19 pages, 5336 KiB

Open AccessArticle

Class III Peroxidases (POD) in Pepper (Capsicum annuum L.): Genome-Wide Identification and Regulation during Nitric Oxide (NO)-Influenced Fruit Ripening

by Salvador González-Gordo, María A. Muñoz-Vargas, José M. Palma and Francisco J. Corpas

Antioxidants 2023, 12(5), 1013; https://doi.org/10.3390/antiox12051013 - 27 Apr 2023

Cited by 8 | Viewed by 2636

Abstract

The class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of H₂O₂ to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little [...] Read more.

The class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of H₂O₂ to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little information is available on sweet pepper fruit physiology. Based on the existing pepper genome, a total of 75 CaPOD genes have been identified, but only 10 genes were found in the fruit transcriptome (RNA-Seq). The time-course expression analysis of these genes showed that two were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Furthermore, nitric oxide (NO) treatment triggered the upregulation of two CaPOD genes whereas the others were unaffected. Non-denaturing PAGE and in-gel activity staining allowed identifying four CaPOD isozymes (CaPOD I-CaPOD IV) which were differentially modulated during ripening and by NO. In vitro analyses of green fruit samples with peroxynitrite, NO donors, and reducing agents triggered about 100% inhibition of CaPOD IV. These data support the modulation of POD at gene and activity levels, which is in agreement with the nitro-oxidative metabolism of pepper fruit during ripening, and suggest that POD IV is a target for nitration and reducing events that lead to its inhibition. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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15 pages, 3859 KiB

Open AccessArticle

Genome-Wide Identification of Superoxide Dismutase and Expression in Response to Fruit Development and Biological Stress in Akebia trifoliata: A Bioinformatics Study

by Huai Yang, Qiuyi Zhang, Shengfu Zhong, Hao Yang, Tianheng Ren, Chen Chen, Feiquan Tan, Guoxing Cao, Jun Liu and Peigao Luo

Antioxidants 2023, 12(3), 726; https://doi.org/10.3390/antiox12030726 - 15 Mar 2023

Cited by 2 | Viewed by 1781

Abstract

Akebia trifoliata is a newly domesticated perennial fruit tree, and the lack of molecular research on stress resistance seriously affects its genetic improvement and commercial value development. Superoxide dismutase (SOD) can effectively eliminate the accumulation of reactive oxygen species (ROS) during [...] Read more.

Akebia trifoliata is a newly domesticated perennial fruit tree, and the lack of molecular research on stress resistance seriously affects its genetic improvement and commercial value development. Superoxide dismutase (SOD) can effectively eliminate the accumulation of reactive oxygen species (ROS) during the rapid growth of plant organs under biotic and abiotic stresses, maintaining a steady state of physiological metabolism. In this study, 13 SODs consisting of two FeSODs (FSDs), four MnSODs (MSDs) and seven Cu/ZnSODs (CSDs) were identified in the A. trifoliata genome. Structurally, the phylogeny, intron–exon pattern and motif sequences within these three subfamilies show high conservation. Evolutionarily, segmental/wide genome duplication (WGD) and dispersed duplication form the current SOD profile of A. trifoliata. Weighted gene coexpression network analysis (WGCNA) revealed the metabolic pathways of nine (69.2%) SODs involved in fruit development, among which AktMSD4 regulates fruit development and AktCSD4 participates in the stress response. In addition, under the stress of multiple pathogens, six (46.6%) SODs were continuously upregulated in the rinds of resistant lines; of these, three SODs (AktMSD1, AktMSD2 and AktMSD3) were weakly or not expressed in susceptible lines. The results pave the way for theoretical research on SODs and afford the opportunity for genetic improvement of A. trifoliata. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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17 pages, 1964 KiB

Open AccessArticle

Noninvasive Methods to Detect Reactive Oxygen Species as a Proxy of Seed Quality

by Adriano Griffo, Nicola Bosco, Andrea Pagano, Alma Balestrazzi and Anca Macovei

Antioxidants 2023, 12(3), 626; https://doi.org/10.3390/antiox12030626 - 03 Mar 2023

Cited by 1 | Viewed by 1869

Abstract

ROS homeostasis is crucial to maintain radical levels in a dynamic equilibrium within physiological ranges. Therefore, ROS quantification in seeds with different germination performance may represent a useful tool to predict the efficiency of common methods to enhance seed vigor, such as priming [...] Read more.

ROS homeostasis is crucial to maintain radical levels in a dynamic equilibrium within physiological ranges. Therefore, ROS quantification in seeds with different germination performance may represent a useful tool to predict the efficiency of common methods to enhance seed vigor, such as priming treatments, which are still largely empirical. In the present study, ROS levels were investigated in an experimental system composed of hydroprimed and heat-shocked seeds, thus comparing materials with improved or damaged germination potential. A preliminary phenotypic analysis of germination parameters and seedling growth allowed the selection of the best-per-forming priming protocols for species like soybean, tomato, and wheat, having relevant agroeconomic value. ROS levels were quantified by using two noninvasive assays, namely dichloro-dihydro-fluorescein diacetate (DCFH-DA) and ferrous oxidation-xylenol orange (FOX-1). qRT-PCR was used to assess the expression of genes encoding enzymes involved in ROS production (respiratory burst oxidase homolog family, RBOH) and scavenging (catalase, superoxide dismutase, and peroxidases). The correlation analyses between ROS levels and gene expression data suggest a possible use of these indicators as noninvasive approaches to evaluate seed quality. These findings are relevant given the centrality of seed quality for crop production and the potential of seed priming in sustainable agricultural practices. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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19 pages, 3909 KiB

Open AccessArticle

The Early Oxidative Stress Induced by Mercury and Cadmium Is Modulated by Ethylene in Medicago sativa Seedlings

by María Laura Flores-Cáceres, Cristina Ortega-Villasante, Pablo Carril, Juan Sobrino-Plata and Luis E. Hernández

Antioxidants 2023, 12(3), 551; https://doi.org/10.3390/antiox12030551 - 22 Feb 2023

Cited by 2 | Viewed by 1977

Abstract

Cadmium (Cd) and mercury (Hg) are ubiquitous soil pollutants that promote the accumulation of reactive oxygen species, causing oxidative stress. Tolerance depends on signalling processes that activate different defence barriers, such as accumulation of small heat sock proteins (sHSPs), activation of antioxidant enzymes, [...] Read more.

Cadmium (Cd) and mercury (Hg) are ubiquitous soil pollutants that promote the accumulation of reactive oxygen species, causing oxidative stress. Tolerance depends on signalling processes that activate different defence barriers, such as accumulation of small heat sock proteins (sHSPs), activation of antioxidant enzymes, and the synthesis of phytochelatins (PCs) from the fundamental antioxidant peptide glutathione (GSH), which is probably modulated by ethylene. We studied the early responses of alfalfa seedlings after short exposure (3, 6, and 24 h) to moderate to severe concentration of Cd and Hg (ranging from 3 to 30 μM), to characterize in detail several oxidative stress parameters and biothiol (i.e., GSH and PCs) accumulation, in combination with the ethylene signalling blocker 1-methylcyclopropene (1-MCP). Most changes occurred in roots of alfalfa, with strong induction of cellular oxidative stress, H₂O₂ generation, and a quick accumulation of sHSPs 17.6 and 17.7. Mercury caused the specific inhibition of glutathione reductase activity, while both metals led to the accumulation of PCs. These responses were attenuated in seedlings incubated with 1-MCP. Interestingly, 1-MCP also decreased the amount of PCs and homophytochelatins generated under metal stress, implying that the overall early response to metals was controlled at least partially by ethylene. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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17 pages, 1897 KiB

Open AccessArticle

Enzymatic and Non-Enzymatic Antioxidant Responses of Young Tomato Plants (cv. Micro-Tom) to Single and Combined Mild Nitrogen and Water Deficit: Not the Sum of the Parts

by Joana Machado, Marta W. Vasconcelos, Cristiano Soares, Fernanda Fidalgo, Ep Heuvelink and Susana M. P. Carvalho

Antioxidants 2023, 12(2), 375; https://doi.org/10.3390/antiox12020375 - 04 Feb 2023

Cited by 6 | Viewed by 2213

Abstract

This study aims to perform a broad analysis of the antioxidant (AOX) responses of young tomato plants exposed to single and combined mild nitrogen (N) and water deficits through the evaluation of oxidative biomarkers, non-enzymatic and enzymatic AOX components. ‘Micro-Tom’ seedlings were subjected [...] Read more.

This study aims to perform a broad analysis of the antioxidant (AOX) responses of young tomato plants exposed to single and combined mild nitrogen (N) and water deficits through the evaluation of oxidative biomarkers, non-enzymatic and enzymatic AOX components. ‘Micro-Tom’ seedlings were subjected to four treatments: control (CTR; 100%N + 100%W), N deficit (N; 50%N), water deficit (W; 50%W), and combined deficits (N + W; 50%N + 50%W). An enhancement of several non-enzymatic and enzymatic components was found in plants subjected to N + W deficit, which presented higher anthocyanins accumulation (up to 103%) as well as higher levels of superoxide dismutase (SOD) transcripts at root level and of ascorbate peroxidase (APX) and catalase (CAT) transcripts at shoot level. This increase in the gene expression was also translated in augmented SOD (up to 202%), APX (up to 155%) and CAT (up to 108%) activity compared to CTR plants and the single deficits. Overall, tomato plants were able to employ defense strategies to cope with this combined deficit, as demonstrated by the higher total AOX capacity (up to 87%) compared to the single deficits, which contributed to the maintenance of their redox homeostasis, with unchanged values of lipid peroxidation and hydrogen peroxide compared with CTR plants. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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Review

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17 pages, 2080 KiB

Open AccessReview

Adventitious Root Formation in Plants: The Implication of Hydrogen Peroxide and Nitric Oxide

by Peter Anargyrou Roussos

Antioxidants 2023, 12(4), 862; https://doi.org/10.3390/antiox12040862 - 02 Apr 2023

Cited by 4 | Viewed by 3531

Abstract

Adventitious root formation is defined as the formation of new roots on above-ground plant parts and is considered crucial for the survival of a plant under harsh environmental conditions (i.e., flooding, salt stress, and other abiotic stresses) as well as in the nursery [...] Read more.

Adventitious root formation is defined as the formation of new roots on above-ground plant parts and is considered crucial for the survival of a plant under harsh environmental conditions (i.e., flooding, salt stress, and other abiotic stresses) as well as in the nursery industry. Clonal propagation is based on the ability of a plant part to grow and generate a completely new plant, genetically identical to the mother plant, where the plant part came from. Nurseries exploit this potential by multiplying millions of new plants. Most nurseries use cuttings to achieve that, through the induction of adventitious root formation. Many factors have been implicated in the capacity of a cutting to root, with the major role being played by auxins. During the last few decades, intense interest has emerged in the role of other potential rooting co-factors, such as carbohydrates, phenolics, polyamines, and other plant growth regulators, as well as signal molecules, such as reactive oxygen and nitrogen species. Among the latter, hydrogen peroxide and nitric oxide have been found to play significant roles in adventitious root formation. Their production, action, and general implication in rhizogenesis are discussed in this review, in terms of interaction with other molecules and signaling. Full article

(This article belongs to the Special Issue Reactive Oxygen and Nitrogen Species in Plants)

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