Monoclonal Antibody Preparation against Natural Product and Its Application
A special issue of Antibodies (ISSN 2073-4468).
Deadline for manuscript submissions: closed (31 August 2012) | Viewed by 79261
As the rapid development of the molecular biosciences and their biotechnological application, immunoassay system using monoclonal antibody (MAb) against drugs and bioactive natural products having small molecular weight have become important tools for studies on quantitative and/or qualitative analytical techniques, enzyme assay and receptor binding analysis owing to their specific affinity. However, since direct immunization for small molecular natural product is impossible, conjugate synthesis of natural products with carrier protein is necessary at the first step. MAb can be successful obtained by immunization of mice, hybridoma preparation, selection and cloning of hybridoma and culturing. Although high specific MAb in general will be required, wide-cross reactive MAb is also useful for analysis of total related components. Qualitative and quantitative analysis of bioactive natural product is necessary for the quality control of herbal medicines. Immunostaining like eastern blotting can determine and identify bioactive compounds specifically in crude extract resulting in fingerprinting of herb medicine. Compact MAb, Fc-engineered antibody can be used as a neutralized antibody for the plant breeding. A special issues with focus on “Monoclonal Antibody Preparation against Natural Product and Its Application” will review recent developments and application of MAbs against natural products having small molecule and further accelerate the investigation around MAb against natural product.
Prof. Dr. Yukihiro Shoyama
- monoclonal antibody
- natural product having small molecule
- quantitative and qualitative analysis
- finger print analysis
- eastern blotting
- immunoaffinity column
- single chain Fv
- Fc-engineered antibody