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Antibiotics
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Updates on Major Food-Related Pathogens: Antimicrobial Treatment, Drug Resistance, Inactivation,...
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Updates on Major Food-Related Pathogens: Antimicrobial Treatment, Drug Resistance, Inactivation, and Related Diseases

A special issue of Antibiotics (ISSN 2079-6382).

Deadline for manuscript submissions: closed (30 January 2024) | Viewed by 2571

Special Issue Editor

Dr. Ramon Silva Rocha

E-Mail Website
Guest Editor
1. Department of Food, Federal Institute of Rio de Janeiro (IFRJ), Food Department, CEP 20270-021 Rio de Janeiro, RJ, Brazil
2. Food Departament, Federal Fluminense University (UFF), Niterói, RJ, Brazil
Interests: food safety; emerging technologies; ohmic heating; pathogenic microorganisms; dairy science; dairy technology; probiotics; prebiotics

Special Issue Information

Dear Colleagues,

Pathogenic microorganisms are a significant concern for the food industry, as they can generate severe damage to consumer health and economic losses to industries. In food processing, there is often a conducive environment for microbiological development.

In this way, strict control of all processing steps is necessary, as well as control of transport and storage steps. Using technologies and processes to eliminate pathogenic microorganisms is essential to ensure food safety. In addition, the identification of pathogenic microorganisms that can contaminate the product is vital to having a quality product.

This Special Issue aims to gather manuscripts to clarify the main pathogens associated with food, their health complications, ways of acting, and technologies or processes applied to the elimination of pathogenic microorganisms in food.

Best regards,

Dr. Ramon Silva Rocha
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibiotics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

20 pages, 3002 KiB  
Article
Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by Enterococcus lactis LBM BT2 Using Alternative Medium with Sugarcane Molasses
by Taciana Freire de Oliveira, Taís Mayumi Kuniyoshi, Elionio Galvão Frota, Sebastián Bermúdez-Puga, Letícia Naomy Sakaue, Luara Lucena Cassiano, Leonardo Tachibana, Rosane Aparecida Moniz Piccoli, Attilio Converti and Ricardo Pinheiro de Souza Oliveira
Antibiotics 2024, 13(3), 210; https://doi.org/10.3390/antibiotics13030210 - 23 Feb 2024
Viewed by 885
Abstract
Listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. [...] Read more.
Listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. In this context, this study aimed to evaluate the anti-listerial action of bacteriocins produced by Enterococcus lactis LBM BT2 in an alternative medium containing sugarcane molasses (SCM). Molecular analyses were carried out to characterize the strain, including the presence of bacteriocin-related genes. In the kinetic study on SCM medium E. lactis, LBM BT2 showed biomass and bacteriocin productions similar to those observed on a sucrose-based medium (control), highlighting the potential of the sugarcane molasses as a low-cost substrate. Stability tests revealed that the molecule remained active in wide ranges of pH (4–10) and temperature (60–100 °C). Furthermore, the proteolytic treatment reduced the biomolecule’s antimicrobial activity, highlighting its proteinaceous nature. After primary purification by salting out and tangential flow filtration, the bacteriocin-like inhibitory substance (BLIS) showed bacteriostatic activity on suspended L. monocytogenes cells and against biofilm formation at a concentration of 0.625 mg/mL. These results demonstrate the potential of the produced BLIS as a biopreservative in the food industry. Full article
(This article belongs to the Special Issue Updates on Major Food-Related Pathogens: Antimicrobial Treatment, Drug Resistance, Inactivation, and Related Diseases)
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10 pages, 486 KiB  
Article
Characterisation of Staphylococci Isolated from Milk Samples of a Water Buffalo Herd
by Christiaan Labuschagne, Joanne Karzis, Hans Britz and Inge-Marié Petzer
Antibiotics 2022, 11(11), 1609; https://doi.org/10.3390/antibiotics11111609 - 12 Nov 2022
Cited by 1 | Viewed by 1243
Abstract
Water buffalo produce a tenth of milk for global human consumption. Non-aureus staphylococci (NAS) are among the most commonly isolated bacteria from mastitis in water buffalo and dairy cows. These results described the initial characterisation of 17 NAS—15 Staphylococcus simulans and two Staphylococcus [...] Read more.
Water buffalo produce a tenth of milk for global human consumption. Non-aureus staphylococci (NAS) are among the most commonly isolated bacteria from mastitis in water buffalo and dairy cows. These results described the initial characterisation of 17 NAS—15 Staphylococcus simulans and two Staphylococcus chromogenes from a water buffalo herd (n = 44) in South Africa. The isolates were identified by classical microbiology, MALDI-TOF, and 16S rRNA, and the disc diffusion method determined the antibiotic susceptibility. A multi-locus sequence typing scheme (MLST) was developed to determine S. simulans sequence types (ST), by defining and comparing seven housekeeping gene fragment sequences. Sequence typing confirmed all 15 S. simulans isolates from water buffalo which belonged to a single ST, genetically distant from the six bovine STs isolated from adjacent farms, which also varied, indicating no current bacterial transfer between species. The antibiotic resistance patterns of S. simulans varied between beta-lactams. The mean milk somatic cell count (SCC) for the water buffalo milk samples was 166,500 cells/mL milk. This information offers insights into the epidemiology and comparison among isolates from various origins, which leads to effective proactive mastitis strategies resulting in safe, high-quality dairy products from water buffalo and dairy cows for human consumption. Full article
(This article belongs to the Special Issue Updates on Major Food-Related Pathogens: Antimicrobial Treatment, Drug Resistance, Inactivation, and Related Diseases)
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