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Journals
Animals
Special Issues
Recent Progress in Zebrafish Research
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Recent Progress in Zebrafish Research

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Aquatic Animals".

Deadline for manuscript submissions: closed (15 January 2024) | Viewed by 4335

Special Issue Editors

Dr. Patricia Diogo

E-Mail Website
Guest Editor
Necton S.A., 8700-152 Olhão, Portugal
Interests: cryopreservation; assisted reproductive technology; reproductive biology; semen analysis; semen evaluation reproduction biology; embryo culture in vitro embryo production; semen preservation; oocyte culture; spermatogenesis; sperm biology; embryology; spermatology; oocytes; in vitro fertilization; zebrafish; reproduction; fish diet; fish skeletal development; aquaculture
Dr. Paulo Jorge Gavaia

E-Mail Website
Guest Editor
Centre of Marine Sciences (CCMAR), Faculty of Medicine and Biomedical Sciences in University of Algarve, Portugal and GreenColab, 8005-139 Faro, Portugal
Interests: zebrafish; fish skeletal malformations
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Zebrafish is crucial for modern research, currently representing the second most used model organism worldwide. This species has been used in research since the 1970s, and its applications have escalated ever since its genome was fully sequenced, revealing around 70% of orthologue genes to humans, and the development of target mutagenesis technologies. In recent decades, the scientific community has generated several thousands of transgenic, mutant and wild-type zebrafish lines. These lines are valuable models used on edge research of pressing matters such as toxicology, embryology, genetics, drug development, and human diseases. Today, zebrafish is ubiquitously used in laboratories worldwide; however, there is a noteworthy lack of knowledge on this species’ biology, such as nutrition, husbandry, and behavior, which are essential to establish standardization guidelines crucial for model species, due to their impact on experimental variability. Since 2019, the number of publications in zebrafish research has escalated, targeting not only its usage as a model and the development of new molecular technologies but also the understanding of this species’ biology.

This Special Issue aims to focus on the progress in zebrafish research towards the strengthening of zebrafish experimental reliability as a model species and to highlight the current innovations on this species’ biology and applications as a model.

Dr. Patricia Diogo
Dr. Paulo Jorge Gavaia 
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • zebrafish
  • nutrition
  • husbandry and reproduction
  • disease modeling
  • development
  • regeneration
  • cell transplantation
  • transgenic and mutant lines
  • toxicology
  • behavior

Published Papers (3 papers)

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Research

13 pages, 3104 KiB  
Article
Evaluation of the Anti-Inflammatory and Antioxidant Potential of Cymbopogon citratus Essential Oil in Zebrafish
by Kiara Cândido Duarte da Silva, William Franco Carneiro, Bárbara do Carmo Rodrigues Virote, Maria de Fátima Santos, João Paulo Lima de Oliveira, Tássia Flávia Dias Castro, Suzan Kelly Vilela Bertolucci and Luis David Solis Murgas
Animals 2024, 14(4), 581; https://doi.org/10.3390/ani14040581 - 09 Feb 2024
Viewed by 831
Abstract
This study explored the protective capacity of the essential oil (EO) of Cymbopogon citratus against oxidative stress induced by hydrogen peroxide (H2O2) and the inflammatory potential in zebrafish. Using five concentrations of EO (0.39, 0.78, 1.56, 3.12, and 6.25 [...] Read more.
This study explored the protective capacity of the essential oil (EO) of Cymbopogon citratus against oxidative stress induced by hydrogen peroxide (H2O2) and the inflammatory potential in zebrafish. Using five concentrations of EO (0.39, 0.78, 1.56, 3.12, and 6.25 μg/mL) in the presence of 7.5 mM H2O2, we analyzed the effects on neutrophil migration, caudal fin regeneration, cellular apoptosis, production of reactive oxygen species (ROS), and activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) after 96 h of exposure. A significant decrease in neutrophil migration was observed in all EO treatments compared to the control. Higher concentrations of EO (3.12 and 6.25 μg/mL) resulted in a significant decrease in caudal fin regeneration compared to the control. SOD activity was reduced at all EO concentrations, CAT activity significantly decreased at 3.12 μg/mL, and GST activity increased at 0.78 μg/mL and 1.56 μg/mL, compared to the control group. No significant changes in ROS production were detected. A reduction in cellular apoptosis was evident at all EO concentrations, suggesting that C. citratus EO exhibits anti-inflammatory properties, influences regenerative processes, and protects against oxidative stress and apoptosis. Full article
(This article belongs to the Special Issue Recent Progress in Zebrafish Research)
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14 pages, 5203 KiB  
Article
A Zebrafish Mutant in the Extracellular Matrix Protein Gene efemp1 as a Model for Spinal Osteoarthritis
by Ratish Raman, Mohamed Ali Bahri, Christian Degueldre, Caroline Caetano da Silva, Christelle Sanchez, Agnes Ostertag, Corinne Collet, Martine Cohen-Solal, Alain Plenevaux, Yves Henrotin and Marc Muller
Animals 2024, 14(1), 74; https://doi.org/10.3390/ani14010074 - 24 Dec 2023
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Abstract
Osteoarthritis is a degenerative articular disease affecting mainly aging animals and people. The extracellular matrix protein Efemp1 was previously shown to have higher turn-over and increased secretion in the blood serum, urine, and subchondral bone of knee joints in osteoarthritic patients. Here, we [...] Read more.
Osteoarthritis is a degenerative articular disease affecting mainly aging animals and people. The extracellular matrix protein Efemp1 was previously shown to have higher turn-over and increased secretion in the blood serum, urine, and subchondral bone of knee joints in osteoarthritic patients. Here, we use the zebrafish as a model system to investigate the function of Efemp1 in vertebrate skeletal development and homeostasis. Using in situ hybridization, we show that the efemp1 gene is expressed in the brain, the pharyngeal arches, and in the chordoblasts surrounding the notochord at 48 hours post-fertilization. We generated an efemp1 mutant line, using the CRISPR/Cas9 method, that produces a severely truncated Efemp1 protein. These mutant larvae presented a medially narrower chondrocranium at 5 days, which normalized later at day 10. At age 1.5 years, µCT analysis revealed an increased tissue mineral density and thickness of the vertebral bodies, as well as a decreased distance between individual vertebrae and ruffled borders of the vertebral centra. This novel defect, which has, to our knowledge, never been described before, suggests that the efemp1 mutant represents the first zebrafish model for spinal osteoarthritis. Full article
(This article belongs to the Special Issue Recent Progress in Zebrafish Research)
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20 pages, 2969 KiB  
Article
The Application of Synthetic Flavors in Zebrafish (Danio rerio) Rearing with Emphasis on Attractive Ones: Effects on Fish Development, Welfare, and Appetite
by Federico Conti, Matteo Zarantoniello, Matteo Antonucci, Nico Cattaneo, Mirko Rattin, Gaia De Russi, Giulia Secci, Tyrone Lucon-Xiccato, Adja Cristina Lira de Medeiros and Ike Olivotto
Animals 2023, 13(21), 3368; https://doi.org/10.3390/ani13213368 - 30 Oct 2023
Viewed by 978
Abstract
The aim of the present study was to test synthetic flavors as potential feed attractants in zebrafish (Danio rerio) during early development. Six experimental groups were set up in triplicate: (i) a CTRL group fed a zebrafish commercial diet; (ii) a [...] Read more.
The aim of the present study was to test synthetic flavors as potential feed attractants in zebrafish (Danio rerio) during early development. Six experimental groups were set up in triplicate: (i) a CTRL group fed a zebrafish commercial diet; (ii) a PG group fed a control diet added with Propylene Glycol (PG); (iii) A1+ and A2+ groups fed a control diet added with 1% of the two attractive flavors (A1+ cheese odor made by mixing Propylene Glycol (PG) with the aromatic chemicals trimethyamine, 2-acetylpyrazine, 2-acetylpyridine, and dimethyl sulfide; and A2+ caramel odor, made of PG mixed with the aromatic chemicals vanillin, maltol, cyclotene, acetoin, butyric acid, and capric acid with traces of both gamma-octalactone and gamma-esalactone) or the repulsive flavor (A− coconut odor, made by mixing PG with the aromatic chemicals gamma-eptalactone, gamma-nonalactone, delta-esalactone, and vanillin with trace of both delta-octalactone and maltol), respectively; (iv) an ROT group fed the two attractive diets, each administered singularly in a weekly rotation scheme. All the tested synthetic flavors did not affect the overall health of larval and juvenile fish and promoted growth. Due to the longer exposure time, results obtained from the juvenile stage provided a clearer picture of the fish responses: zebrafish fed both attractive diets showed higher appetite stimulus, feed ingestion, and growth, while the brain dopaminergic activity suggested the A2+ diet as the most valuable solution for its long-lasting effect over the whole experiment (60-day feeding trial, from larvae to adults). The present study provided important results about the possible use of attractive synthetic flavors for aquafeed production, opening new sustainable and more economically valuable opportunities for the aquaculture sector. Full article
(This article belongs to the Special Issue Recent Progress in Zebrafish Research)
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