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Agronomy
Special Issues
In Vitro Multiplication and Seed Production Technology
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In Vitro Multiplication and Seed Production Technology

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: closed (25 October 2021) | Viewed by 9092

Special Issue Editors

Prof. Dr. Youngseok Lim

E-Mail Website
Guest Editor
Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea
Interests: biotechnology; plant tissue culture; seed production; medicinal herbs/woody plant; breeding
Dr. Md Obyedul Kalam Azad

E-Mail Website1 Website2
Guest Editor
Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea
Interests: plant photochemistry and photobiology; artificial light; plant metabolites; plant pigment

Special Issue Information

Dear colleagues,

In vitro micropropagation and value-added transplant production in fungus-free media with minimum resource consumption is a vital segment for both the research and industrial scale. Plant micropropagation is an indispensable tool for high-quality plant production. In vitro propagation allows growing genetically uniform and disease-free plant material. Technology allows us to control the development of plants by regulating numerous parameters that influence seedling quality. Traditionally, the induction of changes in in vitro plant growth and development has investigated by varying micro environmental factors. However, there are still many limitations in micropropagation technology, such as fungus infection in growth media. Thus, the development of a photoautotrophic micropropagation (PMP) system would be a potential study area.

The influence of light is an important signaling component in regulating plant growth and development, especially in PMP systems. In particular, spectral attributes along with their matching ability to different types of photoreceptors are the key factors that govern the morphogenesis of plants.

The production of good-quality seedlings is essential for improving yields and getting quality products. The success of seed germination and seedling quality is the basic pillar of higher yield. At present, all sorts of plants are being grown in a controlled environment (smart farm) under artificial LED light. In optimizing plant growth media as well as environmental factors such as light spectrum, quality is an imperative issue. Improving the rooting capacity of in vitro microcutting is key for mass seedling production. However, the interspecific relationship of the sugar-free medium, light spectrum, and hormone effect rooting formation deserves in-depth study.  

Prof. Dr. Youngseok Lim
Dr. Md Obyedul Kalam Azad
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • photoautotrophic
  • vegetative micropropagation
  • stem cutting of medicinal plants
  • LED light
  • tuber plants
  • smart plant factory
  • growth media
  • in vitro seed germination
  • seed production
  • synthetic seeds
  • in vitro multiplication
  • tissue culture techniques
  • cryopreservation
  • hydroponics
  • in vitro breeding

Published Papers (3 papers)

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Research

17 pages, 5191 KiB  
Article
Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of Pyrus communis
by Michele Carla Nadal, Gustavo Magno dos Reis Ferreira, Gracielle Vidal Silva Andrade, Victor Hugo Buttrós, Filipe Almendagna Rodrigues, Caroline Marcela da Silva, Adalvan Daniel Martins, Léo Rufato, José Magno Queiroz Luz, Joyce Dória and Moacir Pasqual
Agronomy 2022, 12(5), 1226; https://doi.org/10.3390/agronomy12051226 - 19 May 2022
Cited by 3 | Viewed by 2375
Abstract
This study aimed to investigate the presence of beneficial microorganisms in the micropropagation of Pyrus communis rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate their effect on the plant rooting process [...] Read more.
This study aimed to investigate the presence of beneficial microorganisms in the micropropagation of Pyrus communis rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate their effect on the plant rooting process and their behavior during the acclimatization phase. The results showed the presence of endophytic microorganisms in plant tissue cultures of the ‘OH×F87′ and ‘PDW’ selections. A low diversity was observed in the population of microorganisms isolated from the in vitro culture of the ‘OH×F87’and ‘PDW’ selections, with a predominance of the genera Acinetobacter, Bacillus, and Buttiauxella. The selection of promoting microorganisms was performed based on the auxin production test, in which 30.36% of the microorganisms tested positive. In the in vitro inoculation, it was possible to observe the promotion of growth and emission of roots in the pear rootstocks, from the inoculation with bacteria identified as capable of producing auxin. This process can be used to replace the inclusion of synthetic auxin in the productive chain of woody fruit trees propagated by vegetative means, such as pears. None of the bacterial isolates was notably more promising, but the general similarity of treatments containing the A. septicus and A. ursingii strains, with the synthetic auxin treatment, suggests the possibility of its use on a large scale allowing the adoption of the cheaper method. of rooting. This work opens the door for further research using new, more promising microbial isolates, and also for lower-cost microorganism cultivation techniques, such as low-cost media obtained from agro-industrial residues. Full article
(This article belongs to the Special Issue In Vitro Multiplication and Seed Production Technology)
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12 pages, 13321 KiB  
Article
Influence of Substrate Composition and Container Size on the Growth of Tissue Culture Propagated Apple Rootstock Plants
by Jae Kyung Kim, Md. Rayhan Ahmed Shawon, Jin Hee An, Yeo Jong Yun, Soo Jeong Park, Jong Kuk Na and Ki Young Choi
Agronomy 2021, 11(12), 2450; https://doi.org/10.3390/agronomy11122450 - 30 Nov 2021
Cited by 9 | Viewed by 2599
Abstract
Substrate composition and container size are considered crucial for apple rootstock grown in a plug seedling system. This study investigated the effect of substrate material’s proportion and different container volumes on the growth of apple rootstock (M-9) plants propagated by tissue culture. In [...] Read more.
Substrate composition and container size are considered crucial for apple rootstock grown in a plug seedling system. This study investigated the effect of substrate material’s proportion and different container volumes on the growth of apple rootstock (M-9) plants propagated by tissue culture. In substrate composition, three different ratios of peat moss (PM): vermiculite (VL): perlite (PL) at 1:1:1 (S1), 1:2:3 (S2), 3:1:2 (S3) were used. For container size, plants were grown in 1000 mL (C1), 500 mL (C2), and 300 mL (C3) containers filled by 1:1:1 mixture of PM, VL and PL. In both cases, plants were treated eleven weeks in a green house. Our results demonstrate that the plant height, number of leaves, leaf area, shoot fresh weight and root fresh weight of apple rootstock were significantly higher in substrate composition S3 compared with S1 composition. However, chlorophyll content (SPAD) and photosynthesis rate were unaffected by variation of substrate composition. Furthermore, rootstock grown in C1 container showed plant height, number of leaves, leaf area, number of nodes, root length, shoot and root’s fresh and dry weight were significantly higher than those grown in C2 and C3 volume containers. The chlorophyll content and transpiration rate were not significantly affected by the different container volumes. These results suggest that the substrate ratio 3:1:2 of PM:VL:PL and container size 1000 mL were more favorable than other treatments for initial growth and development of the tissue culture propagated apple rootstock plants. Full article
(This article belongs to the Special Issue In Vitro Multiplication and Seed Production Technology)
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16 pages, 3604 KiB  
Article
LED Light Pre-Treatment Improves Pre-Basic Seed Potato (Solanum tuberosum L. cv. Golden King) Production in the Aeroponic System
by Md. Hafizur Rahman, Md. Jahirul Islam, Md. Obyedul Kalam Azad, Md. Soyel Rana, Byeong Ryeol Ryu and Young-Seok Lim
Agronomy 2021, 11(8), 1627; https://doi.org/10.3390/agronomy11081627 - 16 Aug 2021
Cited by 7 | Viewed by 2589
Abstract
Production of plants under artificial light conditions is an innovative and smart concept to grow food year-round in any location. However, pre-basic seed potato production in the greenhouse from LED pre-treated seedlings under an aeroponic system is a new and creative idea. Therefore, [...] Read more.
Production of plants under artificial light conditions is an innovative and smart concept to grow food year-round in any location. However, pre-basic seed potato production in the greenhouse from LED pre-treated seedlings under an aeroponic system is a new and creative idea. Therefore, the objective of the study was to optimize the effect of LED pre-treatment and determine the best LED spectral composition on growth performance and tuberization of potato plants. Potato variety ‘Golden King’ was treated under 9 LED light spectra for 30 days—L1 (natural light), L2, (R:B), L3 (R:B:G), L4 (R:B:FR), L5 (R:B:G:FR), L6 (R:B:G:FR:UV), L7 (R:B:FR:UV), L8 (R:B:W:FR), and L9 (R:B:W:FR:UV) under 300 µmol m−2 s−1 photosynthetic photon flux density (PPFD), 23/15 °C (day/night) temperature, and 70% relative humidity. The study revealed that growth characteristics and tuber number for plants were increased most by the light spectrum L4 (R:B:FR). Furthermore, photosynthetic pigments increased in L4, L7, and L8, while TSC and sucrose accumulated more in L1 treatment. In contrast, higher seed tuber fresh weight was recorded in L8, L9, L4, and L7. Overall, it can be concluded that potato seedlings pre-treated with the L4 (R:B:FR) LED spectral composition performed best for growth, establishment, and tuberization. Full article
(This article belongs to the Special Issue In Vitro Multiplication and Seed Production Technology)
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