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Agriculture
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Advances in Functional Genomics for Crop Improvement
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Advances in Functional Genomics for Crop Improvement

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Genotype Evaluation and Breeding".

Deadline for manuscript submissions: closed (25 March 2023) | Viewed by 5796

Special Issue Editors

Dr. Santosh Kumar Upadhyay

E-Mail Website
Guest Editor
Department of Botany, Panjab University, Chandigarh 160014, India
Interests: agriculture biotechnology; transgenic; functional genomics; gene expression; genome engineering
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Kashmir Singh

E-Mail Website
Guest Editor
Department of Biotechnology, Panjab University, Chandigarh 160014, India
Interests: biotechnology; gene expression; transgenics; bioinformatics; plants stress
Dr. Prashant Misra

E-Mail Website
Guest Editor
Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180001, India
Interests: agriculture biotechnology; metabolomics; secondary metabolites

Special Issue Information

Dear Colleagues,

In recent years, several advancements in functional genomic approaches and tools, especially related to genome editing, have been attained. These tools have been utilized or are being utilized for numerous purposes, including nutritional improvement and stress tolerance in several agricultural and horticultural crop species, including rice, wheat, potato, tomato, oil seed crops, bananas, etc. In this context, advancements in the use of functional genomics and genome engineering tools for crop biofortification for nutritional improvement, nutraceuticals improvement, flavonoid enrichment, yield improvement, disease resistance, virus resistance, abiotic stress tolerance, etc. should be covered in this field. Moreover, the applications of these tools in oil seed crops, vegetables, ornamental plants, etc., should also be included. Furthermore, their application in reducing post-harvest loss in various crops and the biosafety measures and regulatory mechanisms suggested by various regulatory authorities around the world along with their importance and shortcomings should also be discussed.

This Special Issue focuses on the application of advances in functional genomics approaches for the nutritional, quality, and yield improvements in various agricultural and horticultural crops. For this reason, it welcomes highly interdisciplinary quality studies from disparate research fields, including agriculture, horticulture, functional genomics, plant biotechnology, gene cloning, characterization, etc. Original research articles and reviews are accepted.

Dr. Santosh Kumar Upadhyay
Prof. Dr. Kashmir Singh
Dr. Prashant Misra
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agriculture is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • agriculture
  • horticulture
  • functional genomics
  • genome engineering
  • gene cloning

Published Papers (2 papers)

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Research

24 pages, 3788 KiB  
Article
Cold Tolerance Mechanisms in Mungbean (Vigna radiata L.) Genotypes during Germination
by Lekshmi S. Manasa, Madhusmita Panigrahy, Kishore Chandra Panigrahi, Gayatri Mishra, Sanjib Kumar Panda and Gyana Ranjan Rout
Agriculture 2023, 13(2), 315; https://doi.org/10.3390/agriculture13020315 - 28 Jan 2023
Cited by 1 | Viewed by 2880
Abstract
Mungbean or greengram (Vigna radiata) is an important legume crop well known for its high protein with nitrogen-fixing abilities. However, the severe yield loss in mungbean occurs due to susceptibility to low temperatures at all stages of plant growth including germination [...] Read more.
Mungbean or greengram (Vigna radiata) is an important legume crop well known for its high protein with nitrogen-fixing abilities. However, the severe yield loss in mungbean occurs due to susceptibility to low temperatures at all stages of plant growth including germination and is a serious concern for its cultivation and productivity. To select cold-tolerant genotypes, a germination-based screening at 10 °C was performed in a total of 204 germplasms. The study showed that cold stress of the initial 8-days during seedling establishment imposed a negative impact throughout the life of mungbean genotypes, which were reflected in the vegetative and reproductive phase (plant height, days to 50% flowering and pods/plant, seeds/pod, yield/plant, and 100-seed weight). The biplot analysis showed that parameters such as germination rate index, Timson’s index, mean germination time, and coefficient of the velocity of germination are the key influential germination parameters for identifying cold tolerance in the seedling stage. Identified cold-tolerant genotype (PAU911) retained higher rootlet number, leaf area, and increased chlorophyll, carotenoid, and malondialdehyde (MDA) content at 10 °C. Based on the confocal microscopic study, it is noticed that the stomatal density, open pore percentage, and trichome density were significant differences in seedlings exposed to cold stress as compared to non-stress. On the basis of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis, it is observed that a new protein identified as TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE1 (TTL1) (UNIPROT Identifier: LOC106762419) which highly correlated with the cold stress response of in the cold-tolerant genotype. Our study identifies a noble member, TTL1, whose expression has a positive role in cold tolerance response at the protein level in V. radiata. This study will help breeding programs with regard to the sustainable growth of mungbean. Full article
(This article belongs to the Special Issue Advances in Functional Genomics for Crop Improvement)
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19 pages, 3388 KiB  
Article
Genome-Wide Identification of BTB Domain-Containing Gene Family in Grapevine (Vitis vinifera L.)
by Nandni Goyal, Monika Bhuria, Deepika Verma, Naina Garewal and Kashmir Singh
Agriculture 2023, 13(2), 252; https://doi.org/10.3390/agriculture13020252 - 20 Jan 2023
Cited by 1 | Viewed by 1894
Abstract
BTB (broad-complex, tram track and bric-a-brac) proteins have broad functions in different growth processes and biotic and abiotic stresses. However, the biological role of these proteins has not yet been explored in grapevine, which draws our attention towards the BTB gene family. Herein, [...] Read more.
BTB (broad-complex, tram track and bric-a-brac) proteins have broad functions in different growth processes and biotic and abiotic stresses. However, the biological role of these proteins has not yet been explored in grapevine, which draws our attention towards the BTB gene family. Herein, we identified 69 BTB genes (VvBTB) in the Vitis vinifera genome and performed comprehensive in silico analysis. Phylogenetic analysis classified VvBTB proteins into five groups, and further domain analysis revealed the presence of other additional functional domains. The majority of BTB proteins were localized in the nucleus. We also performed differential expression analysis by harnessing RNA- seq data of 10 developmental stages and different biotic and abiotic stresses. Our findings revealed the plausible roles of the BTB gene family in developmental stages; Fifty VvBTB were differentially expressed at different developmental stages. In addition, 47 and 16 VvBTB were responsive towards abiotic and biotic stresses, respectively. Interestingly, 13 VvBTB genes exhibited differential expression in at least one of the developmental stages and biotic and abiotic stresses. Further, miRNA target prediction of 13 VvBTB genes revealed that vvi-miR482 targets VvBTB56, and multiple miRNAs, such as vvi-miR172, vvi-miR169 and vvi-miR399, target VvBTB24, which provides an insight into the essential role of the BTB family in the grapevine. Our study provides the first comprehensive analysis and essential information that can potentially be used for further functional investigation of BTB genes in this economically important fruit crop. Full article
(This article belongs to the Special Issue Advances in Functional Genomics for Crop Improvement)
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