Caffeic Acid and Biopesticides Interactions for the Control of Storage Beetles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Rearing
2.2. Caffeic Acid Solution and Biopesticides
2.3. Experimental Procedure
2.4. Mathematical Estimation and Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Mortality | x2 | Interaction 3 | Mortality | x2 | Interaction | Mortality | x2 | Interaction | Mortality | x2 | Interaction | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Observed% 1 | Expected% 2 | Observed% | Expected% | Observed% | Expected% | Observed% | Expected% | ||||||||||
Entomopathogen (3000 ppm) | caffeic acid (ppm) | 7 days | 14 days | 21 days | 28 days | ||||||||||||
Bacillus thuringiensis4 | 250 | 37 | 55 | 7.0100 | C | 37 | 75 | 18.7841 | C | 43 | 84 | 46.0425 | C | 47 | 89 | 30.6352 | C |
500 | 37 | 63 | 7.0169 | C | 37 | 75 | 18.5344 | C | 47 | 86 | 40.2042 | C | 47 | 89 | 30.0133 | C | |
750 | 37 | 61 | 7.0094 | C | 40 | 75 | 17.4215 | C | 47 | 84 | 34.6456 | C | 53 | 89 | 29.2205 | C | |
1500 | 40 | 61 | 7.0292 | C | 47 | 74 | 15.5904 | C | 47 | 85 | 29.3120 | C | 57 | 89 | 23.8527 | C | |
3000 | 43 | 63 | 7.0450 | C | 57 | 79 | 4.3251 | C | 60 | 85 | 11.9527 | C | 67 | 93 | 18.5516 | C | |
Cydia pomonella Granulovirus (CpGV) 5 | 250 | 40 | 42 | 0.0435 | A | 57 | 67 | 0.7815 | A | 62 | 78 | 3.2709 | A | 69 | 90 | 7.6806 | C |
500 | 57 | 53 | 0.3601 | A | 62 | 67 | 0.0967 | A | 70 | 80 | 1.9105 | A | 77 | 90 | 2.7879 | A | |
750 | 60 | 50 | 1.6065 | A | 65 | 67 | 0.1029 | A | 70 | 78 | 0.8179 | A | 77 | 90 | 2.7879 | A | |
1500 | 67 | 50 | 9.3082 | S | 77 | 72 | 0.5404 | A | 71 | 79 | 0.8291 | A | 81 | 90 | 3.2932 | A | |
3000 | 67 | 53 | 2.7871 | A | 79 | 65 | 3.1823 | A | 83 | 79 | 0.6247 | A | 89 | 94 | 0.5655 | A | |
Beauveria bassiana strain GHA 6 | 250 | 47 | 53 | 0.5122 | A | 57 | 87 | 15.8274 | C | 69 | 97 | 66.4130 | C | 84 | 98 | 16.5679 | C |
500 | 50 | 62 | 1.4289 | A | 83 | 87 | 0.0438 | A | 87 | 97 | 9.3253 | C | 90 | 98 | 4.1772 | C | |
750 | 52 | 59 | 0.3538 | A | 97 | 87 | 3.5782 | A | 93 | 97 | 1.0476 | A | 93 | 98 | 1.0648 | A | |
1500 | 57 | 59 | 0.0292 | A | 100 | 89 | 4.4242 | S | 100 | 97 | 1.0261 | A | 100 | 98 | 1.0154 | A | |
3000 | 60 | 62 | 0.0162 | A | 100 | 86 | 5.6963 | S | 100 | 97 | 1.0261 | A | 100 | 98 | 1.0070 | A |
Treatment | Mortality | x2 | Interaction 3 | Mortality | x2 | Interaction | Mortality | x2 | Interaction | Mortality | x2 | Interaction | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Observed% 1 | Expected% 2 | Observed% | Expected% | Observed% | Expected% | Observed% | Expected% | ||||||||||
Entomopathogen (3000 ppm) | caffeic acid (ppm) | 7 days | 14 days | 21 days | 28 days | ||||||||||||
Bacillus thuringiensis4 | 250 | 20 | 19 | 0.0558 | A | 37 | 50 | 1.4805 | A | 43 | 61 | 3.7692 | A | 63 | 78 | 3.2719 | A |
500 | 30 | 22 | 1.3274 | A | 37 | 51 | 2.2317 | A | 50 | 64 | 2.4033 | A | 80 | 83 | 0.0296 | A | |
750 | 35 | 25 | 1.6483 | A | 50 | 56 | 0.2562 | A | 63 | 68 | 0.2309 | A | 90 | 85 | 0.8895 | A | |
1500 | 47 | 19 | 15.6913 | S | 50 | 58 | 0.6202 | A | 77 | 70 | 1.1977 | A | 93 | 88 | 0.9887 | A | |
3000 | 47 | 25 | 7.7754 | S | 57 | 60 | 0.1210 | A | 83 | 70 | 3.1762 | A | 97 | 90 | 1.6296 | A | |
Cydia pomonella Granulovirus (CpGV) 5 | 250 | 23 | 28 | 0.3229 | A | 40 | 48 | 0.6507 | A | 50 | 53 | 0.0373 | A | 63 | 62 | 0.0688 | A |
500 | 33 | 30 | 0.1053 | A | 57 | 50 | 0.8984 | A | 60 | 56 | 0.3524 | A | 80 | 71 | 1.3673 | A | |
750 | 33 | 34 | 0.0037 | A | 57 | 55 | 0.1206 | A | 60 | 61 | 0.0050 | A | 87 | 74 | 2.7530 | A | |
1500 | 47 | 28 | 5.5592 | S | 60 | 56 | 0.3524 | A | 67 | 63 | 0.4454 | A | 90 | 79 | 2.7072 | A | |
3000 | 47 | 34 | 2.4437 | S | 63 | 59 | 0.3567 | A | 77 | 63 | 3.1074 | A | 97 | 83 | 5.0396 | S | |
Beauveria bassiana strain GHA 6 | 250 | 10 | 31 | 5.9015 | C | 43 | 51 | 0.6802 | A | 77 | 85 | 0.9549 | A | 87 | 95 | 2.0092 | A |
500 | 23 | 33 | 1.1206 | A | 50 | 53 | 0.0492 | A | 87 | 86 | 0.2435 | A | 90 | 96 | 0.6173 | A | |
750 | 23 | 36 | 1.9048 | A | 67 | 57 | 1.2404 | A | 87 | 88 | 0.0046 | A | 90 | 97 | 0.6355 | A | |
1500 | 37 | 31 | 0.5979 | A | 67 | 59 | 1.0598 | A | 97 | 88 | 2.6542 | A | 93 | 97 | 1.2666 | A | |
3000 | 40 | 36 | 0.3271 | A | 70 | 62 | 1.1024 | A | 100 | 88 | 4.4576 | S | 100 | 98 | 1.0154 | A |
Treatment | Mortality | x2 | Interaction 3 | Mortality | x2 | Interaction | Mortality | x2 | Interaction | Mortality | x2 | Interaction | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Observed% 1 | Expected% 2 | Observed% | Expected% | Observed% | Expected% | Observed% | Expected% | ||||||||||
Entomopathogen (3000 ppm) | caffeic acid (ppm) | 7 days | 14 days | 21 days | 28 days | ||||||||||||
Bacillus thuringiensis4 | 250 | 10 | 46 | 14.4489 | C | 57 | 60 | 0.1155 | A | 70 | 79 | 0.8927 | A | 90 | 86 | 0.8651 | A |
500 | 20 | 48 | 9.0018 | C | 67 | 64 | 0.1496 | A | 73 | 79 | 0.3189 | A | 93 | 86 | 1.8692 | A | |
750 | 27 | 51 | 6.5817 | C | 70 | 64 | 0.5271 | A | 81 | 81 | 0.0151 | A | 93 | 86 | 1.8692 | A | |
1500 | 33 | 55 | 5.3190 | C | 70 | 68 | 0.1276 | A | 83 | 84 | 0.0029 | A | 97 | 88 | 2.6660 | A | |
3000 | 33 | 55 | 5.3190 | C | 77 | 72 | 0.6169 | A | 91 | 84 | 1.2601 | A | 100 | 92 | 3.2246 | A | |
Cydia pomonella Granulovirus (CpGV) 5 | 250 | 27 | 20 | 0.9188 | A | 53 | 60 | 0.6191 | A | 67 | 64 | 0.1496 | A | 73 | 70 | 0.4055 | A |
500 | 27 | 23 | 0.3924 | A | 60 | 64 | 0.1692 | A | 73 | 64 | 1.1362 | A | 80 | 70 | 2.0486 | A | |
750 | 30 | 27 | 0.1455 | A | 60 | 64 | 0.1692 | A | 77 | 68 | 1.3612 | A | 87 | 70 | 4.9951 | S | |
1500 | 37 | 33 | 0.3555 | A | 63 | 68 | 0.2114 | A | 83 | 72 | 2.2449 | A | 87 | 75 | 2.6684 | A | |
3000 | 40 | 33 | 0.8740 | A | 67 | 72 | 0.1750 | A | 83 | 72 | 2.2449 | A | 90 | 83 | 1.7042 | A | |
Beauveria bassiana strain GHA 6 | 250 | 20 | 20 | 0.0000 | A | 53 | 67 | 2.5699 | A | 73 | 75 | 0.0142 | A | 83 | 88 | 0.3815 | A |
500 | 23 | 23 | 0.0337 | A | 56 | 70 | 2.8425 | A | 73 | 75 | 0.0142 | A | 93 | 88 | 0.9946 | A | |
750 | 30 | 27 | 0.1455 | A | 67 | 70 | 0.1390 | A | 80 | 77 | 0.1733 | A | 93 | 88 | 0.9946 | A | |
1500 | 37 | 33 | 0.3555 | A | 73 | 73 | 0.0902 | A | 87 | 80 | 0.9188 | A | 97 | 90 | 2.5684 | A | |
3000 | 37 | 33 | 0.3555 | A | 77 | 77 | 0.1522 | A | 87 | 80 | 0.9188 | A | 97 | 93 | 1.5232 | A |
Separate Treatments | Combined Treatments | |||||
---|---|---|---|---|---|---|
Source | df | F | Sig. | df | F | Sig. |
Exposure time | 3 | 11.838 | <0.001 | 3 | 8.142 | <0.001 |
Insect species | 2 | 10.099 | <0.001 | 2 | 6.499 | <0.001 |
Treatment | 3 | 16.476 | <0.001 | 4 | 3.702 | <0.001 |
Exposure time * Insect species | 6 | 11.109 | <0.001 | 6 | 7.288 | <0.001 |
Exposure time * Treatment | 9 | 11.540 | <0.001 | 12 | 11.534 | <0.001 |
Insect Species * Treatment | 6 | 13.829 | <0.001 | 8 | 5.420 | <0.001 |
Exposure time * Insect species * Treatment | 16 | 14.950 | <0.001 | 24 | 9.946 | <0.001 |
Error | 210 | 380 | ||||
Total | 280 | 400 | ||||
Corrected total | 279 | 399 |
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Zarmakoupi, C.; Mpistiolis, K.; Pantazis, G.; Psatha, P.; Dimitriadi, D.; Kitsiou, F.; Eliopoulos, P.; Patakioutas, G.; Mantzoukas, S. Caffeic Acid and Biopesticides Interactions for the Control of Storage Beetles. Appl. Biosci. 2023, 2, 211-221. https://doi.org/10.3390/applbiosci2020015
Zarmakoupi C, Mpistiolis K, Pantazis G, Psatha P, Dimitriadi D, Kitsiou F, Eliopoulos P, Patakioutas G, Mantzoukas S. Caffeic Acid and Biopesticides Interactions for the Control of Storage Beetles. Applied Biosciences. 2023; 2(2):211-221. https://doi.org/10.3390/applbiosci2020015
Chicago/Turabian StyleZarmakoupi, Chrysanthi, Konstantinos Mpistiolis, George Pantazis, Panagiota Psatha, Despoina Dimitriadi, Foteini Kitsiou, Panagiotis Eliopoulos, George Patakioutas, and Spiridon Mantzoukas. 2023. "Caffeic Acid and Biopesticides Interactions for the Control of Storage Beetles" Applied Biosciences 2, no. 2: 211-221. https://doi.org/10.3390/applbiosci2020015