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Abstract

Intrinsic (Genotype) and Extrinsic (Environment) Factors in the Temporal Patterns of Nest-Building Process: Effect of Forced Isolation in Old Female Mice with Normal and AD-Pathological Aging †

1
Institut de Neurociències, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
2
Department of Psychiatry and Forensic Medicine, School of Medicine, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Brain Sciences, 15–30 July 2021. Available online: https://sciforum.net/event/IECBS2021.
Med. Sci. Forum 2022, 8(1), 7; https://doi.org/10.3390/IECBS2021-10677
Published: 14 July 2021
(This article belongs to the Proceedings of The 2nd International Electronic Conference on Brain Sciences)

Abstract

:
The impact of isolation has become a critical worldwide issue since the outbreak of the COVID-19 pandemic. In nursing homes, the physical distance measures forced the separation of old patients in restricted areas and rooms to avoid the spread of the virus. Similarly, older people living at home face severe restrictions as the best preventive strategy to protect their lives before vaccination is possible/effective. At the translational level, we recently demonstrated the impact of isolation in male 3×Tg-AD mice for Alzheimer’s disease and the increase in gross and fine motor activity. The latter was monitored through nesting, a species-typical ethological behavior used as a naturalistic approach to measuring animals’ well-being and abilities in instrumental tasks. In the present work, we scored the nests and the nest-building process in old females under the effects of intrinsic (genotype, 3×Tg-AD vs. C57BL/6J) and extrinsic (environment, forced isolation vs. social environment) factors. Nests were scored according to the ordinal Deacon Scale, whereas the temporal progress of nest construction was determined with a new parametric (numeric) measurement analog. The results confirmed previously described genotype differences, with worse nests in 3×Tg-AD mice living under standard housing conditions than their non-transgenic counterparts, at 48 and 72 h. However, the genotype effect was lost under isolation, mainly due to isolated 3×Tg-AD females enhancing nest-building behavior, while isolated non-transgenic counterparts were less efficient at 24 h. The results also indicate that temporal patterns of the nest-building process are important to be considered when measuring the effects of intrinsic and extrinsic factors.

Supplementary Materials

The following supporting information can be downloaded at: https://sciforum.net/manuscripts/10667/slides.pdf, References [1,2,3,4,5,6,7,8] are cited in the supplementary materials.

Author Contributions

L.G.-L., conceptualization, and supervision; A.R.d.M.G., writing and formal analysis. Both authors revised and approved the final manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

The colonies are currently sustained by Fet-Open ArrestAD European Union’s Horizon 2020 research and innovation program under grant agreement No. 737390 to L.G.-L.

Institutional Review Board Statement

The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Ethics Committee of Departament de Medi Ambient i Habitatge, Generalitat de Catalunya (CEEAH 3588/DMAH 9452) the 8 March 2019.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are not publicly available due to ongoing analyses for future publications.

Acknowledgments

We thank Frank M. LaFerla Institute for Memory Impairments and Neurological Disorders, Department of Neurobiology and Behavior, University of California, Irvine, USA, for kindly providing the progenitors of the Spanish colonies of 3xTg-AD and NTg mice.

Conflicts of Interest

The authors declare no conflict of interest.

References

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  8. Giménez-Llort, L.; Ruiz de Molina García, A. Measuring Temporal Patterns of the Nest-building Process in Mice for Animal Welfare and Disease Monitoring. In Proceedings of the 2nd International Electronic Conference on Brain Sciences, Online, 15–30 July 2021; MDPI: Basel, Switzerland. [Google Scholar] [CrossRef]
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MDPI and ACS Style

García, A.R.d.M.; Giménez-Llort, L. Intrinsic (Genotype) and Extrinsic (Environment) Factors in the Temporal Patterns of Nest-Building Process: Effect of Forced Isolation in Old Female Mice with Normal and AD-Pathological Aging. Med. Sci. Forum 2022, 8, 7. https://doi.org/10.3390/IECBS2021-10677

AMA Style

García ARdM, Giménez-Llort L. Intrinsic (Genotype) and Extrinsic (Environment) Factors in the Temporal Patterns of Nest-Building Process: Effect of Forced Isolation in Old Female Mice with Normal and AD-Pathological Aging. Medical Sciences Forum. 2022; 8(1):7. https://doi.org/10.3390/IECBS2021-10677

Chicago/Turabian Style

García, Ana Ruiz de Molina, and Lydia Giménez-Llort. 2022. "Intrinsic (Genotype) and Extrinsic (Environment) Factors in the Temporal Patterns of Nest-Building Process: Effect of Forced Isolation in Old Female Mice with Normal and AD-Pathological Aging" Medical Sciences Forum 8, no. 1: 7. https://doi.org/10.3390/IECBS2021-10677

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