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Abstract

Pomegranate Juice Ameliorates Dopamine Release and Motor and Olfactory Deficits in The Rotenone-Induced Rat Model of Parkinson’s Disease †

1
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland
2
Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland
3
Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland
4
Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland
*
Author to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Brain Sciences, 15–30 July 2021. Available online: https://sciforum.net/event/IECBS2021.
Med. Sci. Forum 2022, 8(1), 4; https://doi.org/10.3390/IECBS2021-10672
Published: 14 July 2021
(This article belongs to the Proceedings of The 2nd International Electronic Conference on Brain Sciences)

Abstract

:
Pomegranate juice (PJ) is a rich source of ellagitannins, precursors of the colonic metabolite urolithin A believed to contribute to pomegranate’s neuroprotective effect. While many experimental studies involving PJ’s role in Alzheimer’s disease and hypoxic–ischemic brain injury have been carried out, our knowledge of pomegranate’s effects against Parkinson’s disease (PD) is very limited. Previously, we have reported that PJ treatment improved the postural stability, which correlated well with the enhancement of neuronal survival, protection against oxidative damage, and α-synuclein aggregation [1]. Since olfactory and motor deficits are typical symptoms of PD associated with a decreased density of nigral dopaminergic neurons, in this study, we aimed to investigate the capability of PJ for protecting the olfactory, motoric and neurochemical alterations. To evaluate its efficiency, Wistar rats were given the treatment with PJ in a dose of 500 mg/kg b.w./day (i.g.) and injected with ROT (1.3 mg/kg b.w./day, s.c.) from the 11th day. The experiment lasted a total of 45 days, including 10 days’ pre-treatment with PJ and 35 days’ combined treatment with PJ and ROT. After that, we assessed the olfactory discrimination index and vertical and horizontal activities as well as dopamine level in the dissected midbrain and cortex of animals according to protocols we described previously [2]. Our findings provide the first evidence that PJ treatment protects against dopamine depletion in the midbrain and cortex that correlated well with the enhanced olfactory discrimination performance. In addition, the PJ treatment slightly mitigated a motor deficit, as evidenced by the increased vertical activity.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/IECBS2021-10672/s1.

Author Contributions

M.K. conceptualization, in vivo experiment, behavioral tests, dopamine analysis and interpretation, obtaining financing, and manuscript preparation; Ł.W. behavioral tests; M.K.-Ł. UPLC–MS/MS analysis of DA; M.S. behavior tests; A.G. isolation of dopamine; J.J.-L. supervision of in vivo study and manuscript preparation supervision. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Narodowe Centrum Nauki, grant ID 2017/26/D/NZ7/00748.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kujawska, M.; Jourdes, M.; Kurpik, M.; Szulc, M.; Szaefer, H.; Chmielarz, P.; Kreiner, G.; Krajka-Kuźniak, V.; Mikołajczak, P.; Teissedre, P.L.; et al. Neuroprotective Effects of Pomegranate Juice against Parkinson’s Disease and Presence of Ellagitannins-Derived Metabolite-Urolithin A In the Brain. Int. J. Mol. Sci. 2019, 21, 202. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Kujawska, M.; Jourdes, M.; Witucki, Ł.; Karaźniewicz-Łada, M.; Szulc, M.; Górska, A.; Mikołajczak, P.Ł.; Teissedre, P.-L.; Jodynis-Liebert, J. Pomegranate Juice Ameliorates Dopamine Release and Behavioral Deficits in a Rat Model of Parkinson’s Disease. Brain Sci. 2021, 11, 1127. [Google Scholar] [CrossRef] [PubMed]
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MDPI and ACS Style

Kujawska, M.; Witucki, Ł.; Karaźniewicz-Łada, M.; Szulc, M.; Górska, A.; Jodynis-Liebert, J. Pomegranate Juice Ameliorates Dopamine Release and Motor and Olfactory Deficits in The Rotenone-Induced Rat Model of Parkinson’s Disease. Med. Sci. Forum 2022, 8, 4. https://doi.org/10.3390/IECBS2021-10672

AMA Style

Kujawska M, Witucki Ł, Karaźniewicz-Łada M, Szulc M, Górska A, Jodynis-Liebert J. Pomegranate Juice Ameliorates Dopamine Release and Motor and Olfactory Deficits in The Rotenone-Induced Rat Model of Parkinson’s Disease. Medical Sciences Forum. 2022; 8(1):4. https://doi.org/10.3390/IECBS2021-10672

Chicago/Turabian Style

Kujawska, Małgorzata, Łukasz Witucki, Marta Karaźniewicz-Łada, Michał Szulc, Agata Górska, and Jadwiga Jodynis-Liebert. 2022. "Pomegranate Juice Ameliorates Dopamine Release and Motor and Olfactory Deficits in The Rotenone-Induced Rat Model of Parkinson’s Disease" Medical Sciences Forum 8, no. 1: 4. https://doi.org/10.3390/IECBS2021-10672

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