Next Article in Journal
Valorization of Camel Meat and Meat Products in the World and in Algeria
Previous Article in Journal
Teeth Follow-Up throughout the Life of the Dromedary: Zootechnical and Veterinary Importance
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Probiotic Engineering: Resolving How Fermentable Sugars Affect Aggregation, Adhesion, and Aggression in Lactobacillaceae †

1
Department of Chemistry, The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel
2
Azrieli Faculty of Medicine, Bar-Ilan University, Safed 1311502, Israel
3
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel
*
Author to whom correspondence should be addressed.
Presented at Cells, Cells and Nothing but Cells: Discoveries, Challenges and Directions, 6–8 March 2023; Available online: https://cells2023.sciforum.net/.
Biol. Life Sci. Forum 2023, 21(1), 31; https://doi.org/10.3390/blsf2023021031
Published: 4 April 2023

Abstract

:
Lactobacillaceae are Gram-positive and lactic acid-positive (LAB) bacteria that frequently serve as probiotics. LAB strains vary in their responses to different carbohydrates as free-living and biofilm communities. We previously found that fermentable sugars triggered an altered carrying capacity with strain specificity during planktonic growth, calling for adding a buffering system during the formulation of probiotics. In addition, a heterogeneous response to fermentable sugars was manifested in microbial aggregation (measured by image-stream flow cytometry), colony development, and attachment to mucin. Of all the probiotic strains, L. rhamnosus GG (LGG), a prevalent probiotic species, manifested an enhanced survival of self-imposed acid stress, consistent with the enhanced cell wall modulation observed by transmitting electron microscopy and proteomic analysis. A comprehensive proteomic and metabolomic study revealed that the formation of biofilms and aggregation capacity is a specific response to glucose independent of self-imposed acid stress. In contrast, the increased competitiveness and aggression of LGG and other LAB strains towards enteric pathogens were a synergistic outcome of a change in organic acid production, glucose-dependent bacteriocin production, and fermentation-specific volatile production. Our improved resolution into the cellular circuits (metabolome, proteome, and volatilome) of probiotic strains and their interactions can lead to developing novel therapeutic approaches to combat GI tract infections.

Author Contributions

Conceptualization, R.S. and I.K.-G.; resources, M.M., I.K.-G. and O.K.; data curation, I.K.-G.; writing—original draft preparation, I.K.-G.; writing—review and editing, I.K.-G.; visualization, I.K.-G.; supervision, M.M., I.K.-G. and O.K.; project administration, R.S., M.M. and I.K.-G.; funding acquisition, I.K.-G. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by ImoH grant number 713645 to I.K.-G.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Suissa, R.; Meijler, M.; Koren, O.; Kolodkin-Gal, I. Probiotic Engineering: Resolving How Fermentable Sugars Affect Aggregation, Adhesion, and Aggression in Lactobacillaceae. Biol. Life Sci. Forum 2023, 21, 31. https://doi.org/10.3390/blsf2023021031

AMA Style

Suissa R, Meijler M, Koren O, Kolodkin-Gal I. Probiotic Engineering: Resolving How Fermentable Sugars Affect Aggregation, Adhesion, and Aggression in Lactobacillaceae. Biology and Life Sciences Forum. 2023; 21(1):31. https://doi.org/10.3390/blsf2023021031

Chicago/Turabian Style

Suissa, Ronit, Michael Meijler, Omry Koren, and Ilana Kolodkin-Gal. 2023. "Probiotic Engineering: Resolving How Fermentable Sugars Affect Aggregation, Adhesion, and Aggression in Lactobacillaceae" Biology and Life Sciences Forum 21, no. 1: 31. https://doi.org/10.3390/blsf2023021031

Article Metrics

Back to TopTop