Next Article in Journal
Film-Based Hydrogel Designed as Functional Biomaterial for Treatment of Skin Wound
Previous Article in Journal
The First Opsins Prediction in Achatina fulica Genome
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Biotechnological Complex Based on Lipase and β-Cyclodextrin on Hydrolysis of Acylglycerides in Plant Oils and Fats †

1
Faculty of Based Medicine, Lomonosov Moscow State University, Lomonosovsky Avenue 27/1, 119991 Moscow, Russia
2
SkyLad AG, Superlab Suisse Epalinges SA, Route de la Corniche 6, 1066 Epalinges, Switzerland
*
Authors to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Biomolecules: Biomacromolecules and the Modern World Challenges, 1–15 November 2022; Available online: https://iecbm2022.sciforum.net/.
Biol. Life Sci. Forum 2022, 20(1), 2; https://doi.org/10.3390/IECBM2022-13404
Published: 1 November 2022

Abstract

:
The present study aimed to evaluate in vitro enzymatic activity of a novel biotechnological active complex based on natural origin compounds—thermophilic lipase and β-cyclodextrin—for hydrolysis of acylglycerides in plant oils and fats. β-cyclodextrin (β-CD) as an additive has attracted attention for its enhanced stability and efficiency of enzymes. In present study, the effects of β-CD on enzymatic hydrolysis of acylglycerides by thermophilic lipase were investigated by modern methods. The UV-spectroscopy, electron microscopy with TEM and kinetics of enzymatic hydrolysis were compared by the addition of β-CD. The results showed that lipase could produce the highest yield of oleic acid in presence of β-CD after 1 and 3 h. The UV spectroscopy demonstrated that the absorbance and fluorescence of lipase decreased with increasing concentration of β-CD due to surface interaction and change of enzyme configuration. Moreover, electron microscopy with TEM showed that lipase formed a special active conglomerate with β-CD for improving hydrolysis and stability. Dermatology evaluation indicated that this complex, when added to household products, did not affect sensitive skin of hands. Overall results indicate that β-CD could increase enzymatic activity of the lipase against acylglycerides and can be considered as promising composition in ecological household products for regular hand application.

Supplementary Materials

The presentation material of this work is available online at https://www.mdpi.com/article/10.3390/IECBM2022-13404/s1.

Author Contributions

Conceptualization, V.A.F. and G.G.E.; methodology, V.A.F.; software, V.A.F.; validation, V.A.F. and G.G.E.; formal analysis, V.A.F.; investigation, V.A.F.; resources, V.A.F.; data curation, V.A.F.; writing—original draft preparation, V.A.F. and G.G.E.; writing—review and editing, V.A.F.; visualization, V.A.F.; supervision, V.A.F.; project administration, V.A.F. and G.G.E.; funding acquisition, V.A.F. All authors have read and agreed to the published version of the manuscript.

Funding

This research was entirely provided by SkyLab AG (Switzerland).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not publicly available due to internal privacy policy.

Conflicts of Interest

The funder SkyLab AG has a role in the funding, design of study, in collection, analysis and interpretation of data, writing of the manuscript and decision to publish the results. V.A.F. and G.G.E. are employees of SkyLab AG. The authors declare no conflict of interest.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Filatov, V.A.; Evseev, G.G. Biotechnological Complex Based on Lipase and β-Cyclodextrin on Hydrolysis of Acylglycerides in Plant Oils and Fats. Biol. Life Sci. Forum 2022, 20, 2. https://doi.org/10.3390/IECBM2022-13404

AMA Style

Filatov VA, Evseev GG. Biotechnological Complex Based on Lipase and β-Cyclodextrin on Hydrolysis of Acylglycerides in Plant Oils and Fats. Biology and Life Sciences Forum. 2022; 20(1):2. https://doi.org/10.3390/IECBM2022-13404

Chicago/Turabian Style

Filatov, Viktor Andreevich, and Grigoriy Gennadevich Evseev. 2022. "Biotechnological Complex Based on Lipase and β-Cyclodextrin on Hydrolysis of Acylglycerides in Plant Oils and Fats" Biology and Life Sciences Forum 20, no. 1: 2. https://doi.org/10.3390/IECBM2022-13404

Article Metrics

Back to TopTop