A Multi-Faceted Approach to Quantifying Recovery of Stream Phytobenthos Following Acute Herbicide Incidents
Abstract
:1. Introduction
2. Materials and Methods
2.1. Flume Design
2.2. Biofilm Functioning (Activity Measurements)
2.3. Biomass Measurements
2.4. Bacterial Abundance
2.5. Algae and Cyanobacterial Species Composition
2.6. Data Analyses
3. Results
3.1. Short-Term Responses of Herbicides on Phytobenthos
3.1.1. Areal Biomass
3.1.2. Metabolic Responses to Herbicide Treatments
3.2. Longer-Term Responses in Biofilms 4.5 Days Post-Herbicide Removal
3.2.1. Biomass of Autotrophs and Heterotrophs
3.2.2. Enumeration of Bacterial Heterotrophs
3.2.3. Taxonomic Composition of Autotrophs within Biofilms
3.2.4. Taxonomic Analysis of Pennate Diatom Fraction, Based on Cleaned Valves in Phytobenthic Biofilms
3.2.5. Trophic Diatom Index in Relation to Herbicide Application
4. Discussion
4.1. Impacts of Chlorotoluron on Phytobenthic Biofilms
4.2. Impacts of a GBH and GBHC on Phytobenthic Biofilms
4.3. Changes in Species Composition of Phytobenthos
4.4. Implications for Ecological Assessment
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Diatom Guild | Control | Chlorotoluron | Glyphosate | Glyphosate and Chlorotoluron |
---|---|---|---|---|
Adnate/Short-Stalked | ||||
Total biovolume × 106 [µm3 cm−2]: | 23 | 6 | 4 | 2 |
Relative abundance %: | 19.7 | 4.1 | 2.5 | 4.1 |
Erect | ||||
Total biovolume × 106 [µm3 cm−2]: | 33 | 16 | 0 | 0 |
Relative abundance %: | 28.2 | 10.1 | 0.0 | 0.0 |
Motile | ||||
Total biovolume × 106 [µm3 cm−2]: | 42 | 55 | 79 | 36 |
Relative abundance %: | 35.6 | 35.0 | 51.8 | 78.5 |
Filaments | ||||
Total biovolume × 106 [µm3 cm−2]: | 20 | 80 | 69 | 8 |
Relative abundance %: | 16.5 | 50.7 | 45.7 | 17.4 |
Estimated Volume | Control | Chlorotoluron | Glyphosate | Glyphosate and Chlorotoluron | |
---|---|---|---|---|---|
Total number of cells × 106 [cells cm−2] | 0.67 | 0.55 | 1.30 | 1.05 | |
Total biovolume × 106 [µm3 cm−2] | 157.94 | 218.43 | 292.75 | 129.67 | |
[µm3] | ×106 [µm3 cm−2] | ×106 [µm3 cm−2] | ×106 [µm3 cm−2] | ×106 [µm3 cm−2] | |
Desmodesmus sp. | 46 | 0.66 | 0.11 | 7.37 | 1.76 |
Gongrosira (Colony) | 2356 | 3.19 | 0.34 | 0.83 | 0.19 |
Green Flagelate | 248 | 0.30 | 0.00 | 3.67 | 7.15 |
unid. Chlorophyta (spherical) (large) | 1150 | 1.38 | 12.41 | 36.31 | 17.92 |
unid. Chlorophyta (spherical) (medium) | 74 | 11.03 | 8.38 | 24.03 | 17.47 |
unid. Chlorophyta in pairs | 74 | 4.41 | 3.71 | 10.24 | 9.88 |
Pediastrum sp. | 141 | 0.40 | 0.88 | 3.50 | 1.06 |
Scenedesmussp. (groups of 4) (large) | 670 | 6.43 | 25.70 | 13.39 | 6.43 |
Scenedesmus sp. (groups of 4) (medium) | 46 | 3.09 | 2.21 | 10.29 | 12.35 |
Scenedesmus sp. (single) (large) | 670 | 3.21 | 3.21 | 24.90 | 3.21 |
Scenedesmus sp. (single) (medium) | 46 | 1.27 | 1.10 | 2.68 | 2.70 |
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Treatment | Chl. a (µg cm−2) | DM (mg cm−2) | AFDM (mg cm−2) |
---|---|---|---|
CON | 2.98 ± 0.53 | 3.53 ± 0.71 | 1.05 ± 0.14 |
CLT | 2.25 ± 0.39 | 3.46 ± 0.95 | 1.12 ± 0.21 |
GBH | 0.73 ± 0.04 * | 1.94 ± 1.19 * | 0.81 ± 0.21 |
GBHC | 0.67 ± 0.06 * | 1.67 ± 0.48 * | 0.77 ± 0.09 * |
Treatment | Chl. a (µg cm−2) | DM (mg cm−2) | AFDM (mg cm−2) |
---|---|---|---|
CON | 2.24 ± 0.58 | 2.65 ± 0.58 | 0.89 ± 0.10 |
CLT | 2.71 ± 0.79 | 2.48 ± 0.55 | 0.85 ± 0.11 |
GBH | 2.38 ± 0.56 | 1.60 ± 0.35 | 0.78 ± 0.07 |
GBHC | 1.22 ± 0.34 | 1.19 ± 0.42 | 0.70 ± 0.09 |
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Rosenkranz, H.; Kelly, M.G.; Anesio, A.M.; Yallop, M.L. A Multi-Faceted Approach to Quantifying Recovery of Stream Phytobenthos Following Acute Herbicide Incidents. Phycology 2023, 3, 25-46. https://doi.org/10.3390/phycology3010003
Rosenkranz H, Kelly MG, Anesio AM, Yallop ML. A Multi-Faceted Approach to Quantifying Recovery of Stream Phytobenthos Following Acute Herbicide Incidents. Phycology. 2023; 3(1):25-46. https://doi.org/10.3390/phycology3010003
Chicago/Turabian StyleRosenkranz, Helen, Martyn G. Kelly, Alexandre M. Anesio, and Marian L. Yallop. 2023. "A Multi-Faceted Approach to Quantifying Recovery of Stream Phytobenthos Following Acute Herbicide Incidents" Phycology 3, no. 1: 25-46. https://doi.org/10.3390/phycology3010003