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Proceeding Paper

Design, Synthesis and Bioactivity of Benzimidazole–2–Carbamates as Soil–Borne Anti–Fungal Agents †,‡

1
Department of Basic Sciences, College of Health and Applied Sciences, A’Sharqiyah University, 413 Ibra, Oman
2
Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, 132 Muscat, Oman
3
Chemistry Department, College of Science, Sultan Qaboos University, 132 Muscat, Oman
4
Biomolecular NMR, Ruhr University of Bochum, D-44780 Bochum, Germany
*
Author to whom correspondence should be addressed.
Presented at the 24th International Electronic Conference on Synthetic Organic Chemistry, 15 November–15 December 2020; Available online: https://ecsoc-24.sciforum.net/.
Dedicated to the Memory of Prof. Dr. h. c. Wolfgang Voelter.
Chem. Proc. 2021, 3(1), 64; https://doi.org/10.3390/ecsoc-24-08093
Published: 13 November 2020

Abstract

:
The design and synthesis of new, safe and potent molecules to apply against soil-borne pathogens is a critical goal for organic and bio-medicinal chemists. Herein, we designed and synthesized a series of benzimidazole-based carbamate derivatives (7a–f), as soil-borne anti-fungals. The derivatives 7a–f were all synthesized in multi-step reactions with acceptable yields. The structures of 7a–f were all identified and characterized using 1H-NMR, IR, HRMS, and melting point calculations. The final compounds were tested on five soil-borne pathogens. The results of various bio-assays showed that compounds 7a-3, 7a-2, 7b-2, 7a-1 and 7b-1 significantly affected the growth of Pythium aphanidermatum, a serious pathogen affecting vegetable crops worldwide. Compounds 7a-1 and 7b-1 were the most efficacious, which resulted in a 96% growth inhibition in Pythium at 100 mg L−1. In conclusion, we reported the potent carbamate derivatives as soil-borne anti-fungals, and believe that the synthesis of more derivatives related to the current scaffold would be beneficial.

1. Introduction

Heterocyclic chemistry plays a critical role in the design and synthesis of bio-active compounds. Some of the most important heterocycles are benzimidazole and its derivatives. The properties of benzimidazole and its derivatives have been studied over more than 100 years. Benzimidazole derivatives are useful intermediates/sub-units for the development of molecules of pharmaceutical or biological interest. Substituted benzimidazole derivatives have been found to have applications in diverse therapeutic areas such as anti-cancer agents, anti-bacterial agents, anti-fungal agents, anti-inflammatory agents, analgesic agents, anti-HIV agents, anti-oxidant agents, anti-convulsant agents, anti-tubercular agents, anti-diabetic agents, anti-leishmanial agents, anti-histaminic agents, anti-malarial agents, and other medicinal agents [1,2,3,4,5,6,7,8]. One of the most important applications of benzimidazole derivatives is its use as an anti-fungal agent in plants. Soil-borne fungi are some of the most important causes of widespread, serious plant diseases. The spores or mycelia of many of these fungi can overwinter or survive adverse conditions in soil or on plant debris, and once an area has become infested with soil-borne fungi, it is generally difficult to get rid of them. There are multiple reported benzimidazole derivatives that are used as fungicides (Figure 1) [9].
In parallel with benzimidazole-containing fungicides, a series of fungicides involving a fluorine atom have been developed. Fluorinated heterocycles have attracted attention due to fluorine’s ability to act as a polar hydrogen or hydroxyl mimic. The introduction of fluorine at a strategic position of a molecule is a powerful and versatile tool for the development of organic molecules, which gain potential biological activities by changing the steric and electronic mapping of the molecule. The inclusion of fluorine into organic molecules can affect their lipophilicity and thus enhance the rate of cell penetration and transport of a drug to an active site (Figure 2) [10,11,12,13].
Except for the addition of a fluorine atom in the structure of some molecules for exerting anti-fungal activity, using piperazine moiety also led to emergent anti-fungal activities in some structures (Figure 3) [14,15,16,17,18,19].
With respect to the above explanations and in continuation of our efforts to design and synthesize novel anti-microbial agents containing fluorine and piperazine substituents (Figure 4a), we reported the synthesis of a series of benzimidazole derivatives and their bio-activity as anti-fungal agents (Figure 4b).
Cucumber (Cucumis sativus) is the most important greenhouse crop in Oman [20,21]. However, soilborne diseases (i.e., damping-off and vine decline) limit cucumber growth and production. The losses due to these diseases have been reported to exceed 70% in some greenhouses [22,23,24]. Damping-off and vine decline diseases are also limiting factors to the production of cucumbers and other cucurbits in different parts of the world [25,26,27,28].
Damping-off and vine decline diseases are caused by different pathogens, including the Pythium, Rhizoctonia and Fusarium species [27,29,30,31]. Pythium aphanidermatum is the most common causal agent of damping-off disease in cucumbers in Oman [22,32,33]. It is also among the two most common pathogens associated with cucumber vine decline [23]. The pathogen is tolerant to heat and has been found to be associated with cucumber root diseases during most of the year.
The management of Pythium-induced diseases in cucumbers has relied on the use of imported fungicides, biological control and cultural practices [27,34,35,36,37]. Mefenoxam and hymexazol are two common fungicides used to manage Pythium-induced diseases in Oman. Despite their use in different farms, mefenoxam suffers from rapid bio-degradation in soil while resistance to hymexazol has been reported among Pythium species [38,39,40,41]. Biological control is a new area of research in Oman. Some bio-control agents have been isolated from Omani soils and plants and tested against Pythium damping-off disease. These agents include Pseudomonas aeruginosa, Aspergillus terreus, Talaromyces spp. and Trichoderma spp. [42,43,44,45,46]. However, the efficacy of these bio-control agents is limited. Due to limitations in these management methods, it is important to search for new fungicide formulations that can be used to control these diseases.
The objectives of this study were:
  • To synthesize a novel class of 2-carbamate benzimidazoles.
  • To investigate the efficacy of the new fungicide formulations in suppressing the growth of the most common soil-borne pathogens.

2. Results and Discussion

2.1. Chemistry

The synthetic scheme for the target compounds 7a–f is outlined in Scheme 1. The synthetic strategy involved multi-step synthesis. The 2-carbamate benzimidazole derivatives 7a–f were prepared from the o-phenylenediamine 6a–f in a one-pot procedure by reacting with 1,3-bis(substitutedoxycarbonyl)-2-methyl-2-thiopseudourea to produce nine new 2-carbamatebenzimidazoles compounds. The yields of the cyclization reaction ranged from excellent to good.
The chemical structures of the new series of 2-carbamate benzimidazoles 7a–f were elucidated utilizing HRMS, 1H NMR, FTIR and Mp. The new compounds synthesized in this research were characterized using 1H NMR, 13C NMR and IR. In the case of intermediates 6a–f, they were not separated and the crude mixture was used directly for the next step to synthesize the final products. The HRMS spectra of the newly prepared molecules displayed molecular ion peaks at the appropriate m/z values. With FTIR, (NHC=O) was shown as a sharp band in the range of 1743–1716 cm−1. The main characterization techniques for the target carbamates 7a–f are HRMS and 1H NMR spectroscopy.
The new 2-carbamatebenzimidazoles 7a–f have been converted to their hydrochloride salts in an attempt to enhance their aqueous solubility, for the biological investigation, due to their poor solubility in organic solvents.
In order to ensure that the structures were maintained as hydrochloride salts after conversion, LC-MS was performed, and the results showed the correct molecular ion peak.

2.2. Biologic Activity

The six fungicide formulations affected the growth of the five fungi at different rates. The growths of Botrytis and Bipolaris were not affected by any of the fungicide formulations at a concentration of 100 mg L−1. However, the growth of Pythium was significantly affected by 7a-3, 7a-2, 7b-2, 7a-1 and 7b-1 (Figure 5). The benzimidazoles with benzyl derivatives (7a-1 and 7b-1) were the most efficacious fungicide formulations in reducing the growth of Pythium, resulting in a 96% growth inhibition in Pythium at 100 mg·L−1. Fusarium and Alternaria were only affected by the 7b-1 fungicide formulation (Figure 5).

3. Conclusions

In conclusion, in this work we reported the synthesis and bio-activity of seventeen benzimidazole-based carbamate derivatives 7a–f as fungicides. The synthesized compounds exhibited an acceptable activity against soil-borne pathogens. The benzimidazoles with benzyl derivatives (7a-1 and 7b-1) showed very high and promising results and were the most efficacious fungicide formulations in terms of reducing the growth of Pythium. Future studies should focus on the efficacy of this fungicide on other soil-borne pathogens.

Acknowledgments

We want to thank Sultan Qaboos University for financial support of this project. The Research Center (TRC) for funding this project.

Conflicts of Interest

The authors declare that they have no conflict of interest.

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Figure 1. Benzimidazole derivatives used as fungicides.
Figure 1. Benzimidazole derivatives used as fungicides.
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Figure 2. Fluorine-containing fungicides.
Figure 2. Fluorine-containing fungicides.
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Figure 3. Structures containing a piperazine ring with anti-fungal activities.
Figure 3. Structures containing a piperazine ring with anti-fungal activities.
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Figure 4. (a) Previous works; (b) current work.
Figure 4. (a) Previous works; (b) current work.
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Scheme 1. The synthetic pathway to target compounds 7a–f.
Scheme 1. The synthetic pathway to target compounds 7a–f.
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Figure 5. The effect of six fungicide formulations on the growth rates of the Pythium, Fusarium, Alternaria, Bipolaris and Botrytis species. Bars with the same letter in the same fungus category are not significantly different from each other at p < 0.05 (Tukey’s Studentized range test, SAS).
Figure 5. The effect of six fungicide formulations on the growth rates of the Pythium, Fusarium, Alternaria, Bipolaris and Botrytis species. Bars with the same letter in the same fungus category are not significantly different from each other at p < 0.05 (Tukey’s Studentized range test, SAS).
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Al–Harthy, T.; Al-Sadi, A.M.; Zoghaib, W.; Moghadam, E.S.; Stoll, R.; Abdel-Jalil, R. Design, Synthesis and Bioactivity of Benzimidazole–2–Carbamates as Soil–Borne Anti–Fungal Agents †,‡. Chem. Proc. 2021, 3, 64. https://doi.org/10.3390/ecsoc-24-08093

AMA Style

Al–Harthy T, Al-Sadi AM, Zoghaib W, Moghadam ES, Stoll R, Abdel-Jalil R. Design, Synthesis and Bioactivity of Benzimidazole–2–Carbamates as Soil–Borne Anti–Fungal Agents †,‡. Chemistry Proceedings. 2021; 3(1):64. https://doi.org/10.3390/ecsoc-24-08093

Chicago/Turabian Style

Al–Harthy, Thuraya, Abdullah M. Al-Sadi, Wajdi Zoghaib, Ebrahim Saeedian Moghadam, Raphael Stoll, and Raid Abdel-Jalil. 2021. "Design, Synthesis and Bioactivity of Benzimidazole–2–Carbamates as Soil–Borne Anti–Fungal Agents †,‡" Chemistry Proceedings 3, no. 1: 64. https://doi.org/10.3390/ecsoc-24-08093

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