Wild-Type and SOD1-G93A SH-SY5Y under Oxidative Stress: EVs Characterization and Topographical Distribution of Budding Vesicles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Treatments
2.2. MTT Assay
2.3. EVs Isolation by Ultrafiltration Combined with Size-Exclusion Chromatography (UF/SEC)
2.4. Transmission Electron Microscopy
2.5. Western Blot Analysis
2.6. Atomic Force Microscopy Preparation Procedure, Imaging, and Analysis
2.7. Statistical Analysis
3. Results
3.1. TEM Analysis Reveals That Oxidative Stress Differently Affects EVs Secretion of WT and SOD1-G93A SH-SY5Y Cells
3.2. AFM Topographical Analysis Shows a Differential Profiling of Budding Vesicles along the Surface of WT and SOD1-G93A SH-SY5Y Cells under Oxidative Stress
3.3. AFM Topographical Mapping of Central and Distal Regions of WT and SOD1-G93A SH-SY5Y Cells: Detecting the Details of a Differential Budding Profile
3.4. Quantifying the WT and SOD1-G93A SH-SY5Y Cells Differential Budding Profile: Analysis of the Surface Roughness
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sbarigia, C.; Dinarelli, S.; Mura, F.; Buccini, L.; Vari, F.; Passeri, D.; Rossi, M.; Tacconi, S.; Dini, L. Wild-Type and SOD1-G93A SH-SY5Y under Oxidative Stress: EVs Characterization and Topographical Distribution of Budding Vesicles. Appl. Nano 2023, 4, 45-60. https://doi.org/10.3390/applnano4010004
Sbarigia C, Dinarelli S, Mura F, Buccini L, Vari F, Passeri D, Rossi M, Tacconi S, Dini L. Wild-Type and SOD1-G93A SH-SY5Y under Oxidative Stress: EVs Characterization and Topographical Distribution of Budding Vesicles. Applied Nano. 2023; 4(1):45-60. https://doi.org/10.3390/applnano4010004
Chicago/Turabian StyleSbarigia, Carolina, Simone Dinarelli, Francesco Mura, Luca Buccini, Francesco Vari, Daniele Passeri, Marco Rossi, Stefano Tacconi, and Luciana Dini. 2023. "Wild-Type and SOD1-G93A SH-SY5Y under Oxidative Stress: EVs Characterization and Topographical Distribution of Budding Vesicles" Applied Nano 4, no. 1: 45-60. https://doi.org/10.3390/applnano4010004