Plasma, Volume 5, Issue 4 (December 2022) – 12 articles

Heat shock protein 27 (Hsp27) is a cytoprotective molecule and is inducible via oxidative stress. Anti-cancer therapies, such as the recently investigated gas plasma, subject tumor cells to a plethora of reactive oxygen species (ROS). In ovarian tumor microenvironments (TME), immune cells such as monocytes and macrophages can be found in large numbers and are often associated with cancer progression. Therefore, we quantified extracellular Hsp27 of OVCAR-3 and SK-OV-3 cells after gas plasma exposure in vitro. We found Hsp27 to be significantly increased. Following this, we investigated the effects of Hsp27 on THP-1 monocytes. Live cell imaging of Hsp27-treated THP-1 cells showed decelerated cell numbers and a reduction in cell cluster sizes. In addition, reduced metabolic activity and proliferation were identified using flow cytometry. Mitochondrial ROS production decreased. Using multicolor flow cytometry, the expression profile of eight out of twelve investigated cell surface markers was significantly modulated in Hsp27-treated THP-1 cells. A significantly decreased release of IL18 accommodated this. Taken together, our results suggest an immunomodulatory effect of Hsp27 on THP-1 monocytes. These data call for further investigations on Hsp27’s impact on the interplay of ovarian cancer cells and monocytes/macrophages under oxidative stress conditions. Full article

In this study, for the first time, the application of plasma electrolytic polishing (PEP) of porous Nitinol structures, mimicking a trabecular bone structure, that were additively manufactured, is reported. The cube-shaped samples were polished in a diagonal position three different times. The effect of PEP was evaluated in terms of the polishing depth, the effect on sample chemical composition and a possible shift of the phase transition temperature using microscopy, the energy dispersive X-ray spectroscopy (EDX), and the differential scanning calorimetry (DSC) techniques, respectively. The obtained results demonstrated that the PEP technique is suitable for polishing porous structures up to a certain depth into the sample inner structure and does not have any influence on the chemical composition and the phase transformation temperatures. However, small changes in the specific enthalpy were observable among the investigated samples. These changes could be attributed to the sample chemical inhomogeneity, measurement error, and/or differences in sample size and shape. Full article

A model for the simulation of ion beam extraction from an electron-cyclotron resonance ion source is proposed. It is based on the simple fact that charged particles follow magnetic field lines. Therefore, magnetic field lines are used to generate initial conditions for ray-tracing. This model reproduces in simulation experimentally obtained results. The importance of correlations in phase-space caused by the magnetic field is shown in the simulation. This model also describes the physics of space-charge and its compensation in the extracted (fast) ion beam by low-energy electrons. Simulation provides the possibility to test theoretical assumptions, as well as to optimize technical designs. Full article

The demand for high-intensity lasers has grown ever since the invention of lasers in 1960, owing to their applications in the fields of inertial confinement fusion, plasma-based relativistic particle accelerators, complex X-ray and gamma-ray sources, and laboratory astrophysics. To create such high-intensity lasers, free-running lasers were either Q-switched or mode-locked to increase the peak power to the gigawatt range. Later, chirped pulse amplification was developed, allowing the generation of peak power up to ${10}^{12}$ W. However, the next generation of high-intensity lasers might not be able to be driven by the solid-state technology alone as they are already operating close to their damage thresholds. In this scenario, concepts of amplification based on plasmas has the potential to revolutionize the laser industry, as plasma is already a broken-down medium, and hence does not pose any problems related to the damage thresholds. On the other hand, there are many other aspects that need to be addressed before developing technologies based on plasma-based amplification, and they are being investigated via theoretical and numerical methods and supported by several experiments. In this report, we review the prospects of employing plasma as the medium of amplification by utilising stimulated scattering techniques, such as the stimulated Raman scattering (SRS) and stimulated Brillouin scattering (SBS) techniques, to modulate high-power laser pulses, which would possibly be the key to the next generation of high-power lasers. The 1980s saw the commencement of research in this field, and possibilities of obtaining high peak powers were verified theoretically with the help of numerical calculations and simulations. The extent of amplification by these stimulated scattering schemes are limited by a number of instabilities such as forward Raman scattering (FRS), filamentation, etc., and here, magnetised plasma played an important role in counteracting these parasitic effects. The current research combines all these factors to experimentally realise a large-scale plasma-based amplifier, which can impact the high-energy laser industry in the near future. Full article

An 0D model is proposed that makes it possible to estimate the limiting stationary heat loads to the targets covered with liquid lithium (LL) layer, taking into account the effects of vapor shielding by sputtered and evaporated LL and hydrogen recycling. Several models of cooled target substrates are considered in which the LL layer facing the plasma is placed. For the considered substrate models, a parametric analysis of the tolerable stationary heat loads to the target on the substrate thickness, the effective cooling energy per particle of sputtered lithium, and the lithium prompt redeposition factor was carried out. It is shown that, at a small substrate thickness, the choice of the substrate model has a significant impact on the tolerable heat loads. It is also shown that even at unrealistically large values of the effective cooling energy, the dissipation of lithium remains modest. This means that in regimes with a high power coming from the core plasma to the edge, the injection of an additional radiator is required. Finally, it is shown that one of the most effective ways to increase the tolerable stationary heat loads would be to reduce the thickness of the target substrate. Full article

In-orbit additive manufacturing (AM) is a promising approach for fabrication of large structures. It allows to expand and accelerate human space exploration possibilities. Extrusion-based AM was demonstrated in zero gravity, while the realization of such a process in orbit-like vacuum conditions is currently under exploration. Still, a solution for protection of the UV and IR radiation sensitive polymers is needed in order to prevent their early mechanical failure under space conditions. Vacuum arc plasma based process is widely applied on earth for thin protective coating deposition. Its major advantage is its scalability—from tiny size used in electric propulsion to large scale coating devices. The usability of the vacuum arc process in space conditions was shown in electric propulsion applications in nano-satellites. In this work we discuss and demonstrate the integration of vacuum arc process as a post processing step after Fused Filament Fabrication (FFF) for additive manufacturing and functionalization of long polymer structures. Here we address the concept for technical realization, which integrates the vacuum arc into additive manufacturing process chain. More over we present a laboratory prototype, which implements this concept together with a use case, where a previously printed PEEK structure is coated with aluminum based coating suitable for UV radiation protection. Full article

Washing fresh produce using Plasma-activated water recently became a promising eco-friendly alternative to using chemical additives such as Chlorine. We discuss the produce-washing experiments that illustrate that addition of plasma to washing water is a multi-faced phenomena. Not only it increases the sterilization ability of water by killing pathogens, but it also has improved washibility: the ability to remove pathogens from the cleaning surface. We propose an explanation of these features based on the recently discoveries that many physical and chemical properties of water change their temperature dependence between about 35 and 60 degrees Celsius. In particular, heat conductance, light absorption, and surface tension all change their temperature dependence. These drastic changes were associated with water gradually changing its mesoscopic structure: while at the higher temperatures water is a uniform media (amorphous state), at the temperatures below transition it consists of many nano-to-micro-scale clusters (crystalline state). This transition is similar to the second order phase transition. In the present paper we propose that treating water with non-thermal plasma (adding plasma-created active compounds) can lower the temperature of the transition and thus cause a significant change in such physical quantities as surface tension, viscosity, freezing rate, and wettability and washability. Full article

The extensive application of biodegradable polymers in the food packaging industries was partially limited due to poor barrier performances. In the present work, we investigated the improvement of oxygen barrier performances by means of the deposition of a few nanometres of SiOx coatings on Poly(butylene succinate) (PBS) films. The coated samples produced by the plasma-enhanced chemical vapor deposition technique were tested in terms of morphology and composition of the surface and barrier properties. Barrier performances studied as a function of SiOx thickness were greatly improved and a reduction of at least 99% was achieved for oxygen transmission rate. In order to reduce the formation of residual stress between PBS substrate and SiOx coatings, a proper buffer layer (silicon organic SiO_xC_yH_z) was used. Full article

This paper describes the design and operation of a low-cost plasma applicator based on a patented, swirled-type dielectric barrier discharge configuration with a treatment width up to 300 mm. Differences from earlier plasma applicators include: blown cylindrical dielectric barrier discharge, combining the functional properties of the plasma jet systems, arc and corona discharge blown in a single type of universal applicator, and the possibility of treating large areas of samples with cold plasma generated in a certain type of specific process gas mixture chosen according to the type of desired effect. We tested the effect of the plasma on a few materials such as cotton and linen fabrics, glass wafers and printing cardboard, proving that the generated plasma can easily make hydrophilic or hydrophobic surfaces. We also tried the plasma’s sterilizing effect on Escherichia coli (E. coli) bacteria. The results suggest that our plasma system can be successfully applied to medical and biological fields as well, where the removal of bacteria and their fragments is required. Full article

The study compares how acetyl donor molecules tetraacetylethylenediamine (TAED) and pentaacetate glucose (PAG) improve the antibacterial efficacy of solutions activated with a low-temperature atmospheric-pressure argon plasma jet. Plasma activation of solubilised TAED and PAG produce solutions with different chemical compositions and oxidative potentials. Both acetyl donor molecules enhance the hydrogen peroxide (H₂O₂) concentration in solution with TAED being more effective compared to PAG. However, PAG is more effective at forming peracetic acid (PAA) from reaction of its acetyl donor groups with plasma generated H₂O₂. The enhanced oxidative potential of plasma activated TAED and PAG solutions were shown to significantly improve bactericidal activity against common wound pathogens Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus compared to plasma activated water produced without acetyl donors. Furthermore, the oxidative capacity of plasma activated PAG was least affected by the bacterial oxidative defence enzyme catalase, attributed to the high concentration of PAA produced in this formulation. Overall, the above data show that acetyl donors may help improve next generation of antimicrobial formulations produced by plasma, which might help combat increasing problems of antimicrobial resistance. Full article

This paper presents a detailed analysis of underwater electrical discharge parameters in the treatment of chromium (VI) used as a model pollutant to analyze the reduction process by plasma liquid interaction (PLI). Pin-to-pin microsecond discharges were performed in an aqueous Cr(VI) solution and the processes were characterized using electrical measurements, optical imaging and UV-Vis absorption measurements for [Cr(VI)] estimation. For the first time, the total reduction of Cr(VI) was successfully achieved by PLI process and a maximum energy yield of 4.7 × 10⁻⁴ g/kJ was obtained. Parametric studies on electrode geometry, applied voltage, electrodes gap and pulse duration are presented in detail. Finally, an analysis of the process is proposed by comparing our results of the energy yield calculation based on the injected energy with those of the literature and by providing an estimation of the global energy efficiency of the process. Full article

The modelling of ionization potential depression in warm and hot dense plasmas constitutes a real theoretical challenge due to ionic coupling and electron degeneracy effects. In this work, we present a quantum statistical model based on a multi-configuration description of the electronic structure in the framework of Density Functional Theory. We discuss different conceptual issues inherent to the definition of ionization potential depression and compare our results with the famous and widely-used Ecker-Kröll and Stewart-Pyatt models. Full article