Next Article in Journal
Health Text Messaging Intervention to Increase Fruit and Vegetable Intake
Previous Article in Journal
Exogenous Carbohydrate as an Ergogenic Aid: Recent Advances in Dose and Form and Format
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:

Animal Model of Antibiotic Induced Gut Microbiota Dysbiosis †

New Zealand Institute for Plant and Food Research Ltd., Palmerston North 4474, New Zealand
Plant and Food Research, Auckland 1025, New Zealand
Dairy Goat Co-operative (NZ) Ltd., Hamilton 3240, New Zealand
Author to whom correspondence should be addressed.
Presented at the 2018 Nutrition Society of New Zealand Annual Conference, Auckland, New Zealand, 28–30 November 2018.
Proceedings 2019, 8(1), 11;
Published: 5 March 2019
(This article belongs to the Proceedings of 2018 Annual Meeting of the Nutrition Society of New Zealand)
Background: The gut harbors a diverse ecosystem consisting predominantly of bacteria. The gut microbiota and host tissues closely interact and disruption of this relationship causes intestinal dysbiosis which has been linked to asthma, inflammatory bowel disease and metabolic disorders. Gastrointestinal dysbiosis has been induced experimentally using antibiotics but few studies have compared the effects of different antibiotics or length of exposure. This study compared different antibiotic treatments on gut microbiota in newly weaned rats to establish a small animal model of gastrointestinal dysbiosis.
Methods: Male Sprague-Dawley rats (3 weeks old) were randomly allocated to the experimental treatments (n = 6): control (no antibiotic), amoxicillin or antibiotic mixture (amoxicillin, gentamicin, metronidazole). The rats were given ad libitum access to food and water throughout the study. The antibiotics were administered in the drinking water for 7 and 14 days. The rats were euthanased at the end of the experimental period and the gut contents and tissues removed for analysis. These procedures were approved by the AgResearch Grasslands Animal Ethics Committee (Application 13948).
Results: Amoxicillin treatment resulted in significantly lower numbers of Bifidobacterium spp. in the caecum, colon and faeces, while higher numbers of Lactobacillus spp. were detected in the caecum of these rats. The antibiotic mixture significantly decreased the Bifidobacterium spp., Lactobacillus spp. and total bacteria in the caecum, colon and faeces. Changes induced by the antibiotics were greater when the rats were treated with the antibiotic mixture than with amoxicillin, and greater after 14 days than after 7 days of antibiotic treatment.
Conclusions: Intestinal dysbiosis was successfully established under these experimental conditions. Future studies will characterize the effect of foods and diet on gut microbiota dysbiosis.

Share and Cite

MDPI and ACS Style

Butts, C.A.; Paturi, G.; Stoklosinski, H.; Martell, S.; Hedderley, D.; Carpenter, E. Animal Model of Antibiotic Induced Gut Microbiota Dysbiosis. Proceedings 2019, 8, 11.

AMA Style

Butts CA, Paturi G, Stoklosinski H, Martell S, Hedderley D, Carpenter E. Animal Model of Antibiotic Induced Gut Microbiota Dysbiosis. Proceedings. 2019; 8(1):11.

Chicago/Turabian Style

Butts, Christine A., Gunaranjan Paturi, Halina Stoklosinski, Sheridan Martell, Duncan Hedderley, and Elizabeth Carpenter. 2019. "Animal Model of Antibiotic Induced Gut Microbiota Dysbiosis" Proceedings 8, no. 1: 11.

Article Metrics

Back to TopTop