Next Article in Journal
The Effect of High Pressure Processing on Antioxidant Activity of Irish Potato Cultivars
Previous Article in Journal
Oxidative Stress Applied in Diabetes Mellitus-A New Paradigm
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Proceeding Paper

Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study †

1
Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy
2
IRCCS INRCA, Geriatria, Accettazione Geriatrica e Centro di Ricerca per l’Invecchiamento, 60127 Ancona, Italy
3
Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UG, UK
4
Biomarkers and Nutrimetabolomics Laboratory, Department of Nutrition, Food Sciences and Gastronomy, XaRTA, INSA, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain
5
CIBER Fragilidad y Envejecimiento Saludable (CIBERfes), Instituto de Salud Carlos III, 28029 Madrid,Spain
*
Author to whom correspondence should be addressed.
Presented at Natural Products and the Hallmarks of Chronic Diseases—COST Action 16112, Luxemburg, 25–27 March 2019.
Proceedings 2019, 11(1), 8; https://doi.org/10.3390/proceedings2019011008
Published: 16 April 2019
(This article belongs to the Proceedings of CA16112 - Luxemburg 2019)

1. Introduction

The inevitable rise of the proportion of people aged >65 years worldwide is paralleled by an increased burden of chronic diseases often associated with low-grade systemic inflammation. Recent findings suggest a link between inflammation and intestinal permeability (IP), a condition characterized by an impairment of intestinal barrier function which enables the translocation of dietary and bacterial factors into the blood activating the host immune system [1,2]. Dietary components can be significant modulators of inflammation and IP, and can also affect the intestinal microbial ecosystem. In the context of a diet-microbiota-IP axis in older subjects, dietary bioactives such as polyphenols may play a significant protective role due to their widely reported antioxidant and immunomodulatory properties and potential to regulate IP [3,4,5,6].

2. Material and Methods

The MaPLE project involves a multidisciplinary approach developed to ascertain the impact of a polyphenol-rich dietary pattern on a large number of markers in a target group of older subjects living in a controlled setting (i.e., nursing home).
A controlled, randomized cross-over dietary intervention study (8-week polyphenol-rich diet versus 8-week control diet) was undertaken. Markers of IP, inflammation, oxidative stress and vascular function and assessments of gut microbiota structure and function were quantified in serum, urine and/or fecal samples. In addition, bacterial DNAemia, and serum/urine metabolomics were assessed. In vivo with a dietary mixture similar to the human study and in vitro studies with isolated polyphenols were carried out to investigate mechanisms of action.

3. Results & Discussion

The dietary intervention has been completed and as expected, IP was relatively high in this cohort of older participants, as assessed by serum levels of zonulin at baseline. Quantification of changes in various markers in response to the high polyphenol diet compared to the normal polyphenol diet are being completed and will provide evidence of the putative beneficial effect of increased polyphenol consumption in this target population.

Funding

This research was supported by a grant by Ministero delle Politiche Agricole, Alimentari, Forestali e del Turismo (Mipaaft), MINECO-Grant MAPLE-PCIN-2015-238, Biotechnology and Biological Sciences Research Council (UK) Grant BB/R012512/1 and the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI- HDHL: http://www.healthydietforhealthylife.eu/) MaPLE. CAL thanks 2017SGR1546 from AGAUR, CIBERFES (co-funded by the FEDER Program from EU) and ICREA Academia award 2018.

Acknowledgments

This publication is based upon work from COST Action NutRedOx-CA16112 supported by COST (European Cooperation in Science and Technology).

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. Thevaranjan, N.; Puchta, A.; Schulz, C.; Naidoo, A.; Szamosi, J.C.; Verschoor, C.P.; Loukov, D.; Schenck, L.P.; Jury, J.; Foley, K.P.; et al. Age-Associated microbial dysbiosis promotes intestinal permeability, systemic inflammation, and macrophage dysfunction. Cell Host Microbe. 2017, 21, 455–466. [Google Scholar] [CrossRef] [PubMed]
  2. Bischoff, S.C.; Barbara, G.; Buurman, W.; Ockhuizen, T.; Schulzke, J.D.; Serino, M.; Tilg, H.; Watson, A.; Wells, J.M. Intestinal permeability—A new target for disease prevention and therapy. BMC Gastroenterol. 2014, 14, 189. [Google Scholar] [CrossRef] [PubMed]
  3. Del Rio, D.; Rodriguez-Mateos, A.; Spencer, J.P.; Tognolini, M.; Borges, G.; Crozier, A. Dietary (poly)phenolics in human health: Structures, bioavailability, and evidence of protective effects against chronic diseases. Antiox. Redox Sig. 2013, 18, 1818–1892. [Google Scholar] [CrossRef] [PubMed]
  4. Taverniti, V.; Fracassetti, D.; Del Bo’, C.; Lanti, C.; Minuzzo, M.; Klimis-Zacas, D.; Riso, P.; Guglielmetti, S. Immunomodulatory effect of a wild blueberry anthocyanin-rich extract in human Caco-2 intestinal cells. J. Agric. Food Chem. 2014, 62, 8346–8351. [Google Scholar] [CrossRef] [PubMed]
  5. Suzuki, T. Regulation of intestinal epithelial permeability by tight junctions. Cell Mol. Life Sci. 2013, 70, 631–659. [Google Scholar] [CrossRef] [PubMed]
  6. Ruan, Z.; Liu, S.; Zhou, Y.; Mi, S.; Liu, G.; Wu, X.; Yao, K.; Assaad, H.; Deng, Z.; Hou, Y.; et al. Chlorogenic acid decreases intestinal permeability and increases expression of intestinal tight junction proteins in weaned rats challenged with LPS. PLoS ONE 2014, 9, e97815. [Google Scholar] [CrossRef] [PubMed]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Bernardi, S.; Del Bo’, C.; Guglielmetti, S.; Cherubini, A.; Kroon, P.; Kirkup, B.; Liberona, N.H.; Peron, G.; González-Domíinguez, R.; Andrés-Lacueva, C.; et al. Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study. Proceedings 2019, 11, 8. https://doi.org/10.3390/proceedings2019011008

AMA Style

Bernardi S, Del Bo’ C, Guglielmetti S, Cherubini A, Kroon P, Kirkup B, Liberona NH, Peron G, González-Domíinguez R, Andrés-Lacueva C, et al. Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study. Proceedings. 2019; 11(1):8. https://doi.org/10.3390/proceedings2019011008

Chicago/Turabian Style

Bernardi, Stefano, Cristian Del Bo’, Simone Guglielmetti, Antonio Cherubini, Paul Kroon, Benjamin Kirkup, Nicole Hidalgo Liberona, Gregorio Peron, Raúl González-Domíinguez, Cristina Andrés-Lacueva, and et al. 2019. "Role of a Polyphenol-Rich Dietary Pattern in the Modulation of Intestinal Permeability in Older Subjects: The MaPLE Study" Proceedings 11, no. 1: 8. https://doi.org/10.3390/proceedings2019011008

Article Metrics

Back to TopTop