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A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies

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Journal: Methods Protoc., 2018
Volume: 1
Number: 28

Article: Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors
Authors: by Gemma Moir-Meyer, Pak Leng Cheong, Aude-Anais Olijnik, Jill Brown, Samantha Knight, Andrew King, Ryo Kurita, Yukio Nakamura, Richard J. Gibbons, Douglas R. Higgs, Veronica J. Buckle and Christian Babbs

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