Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Identification of Strains
2.2. Metabolite Analysis
2.3. Genome Sequencing, Assembly and Annotation
2.4. Total RNA Extraction, Reverse Transcription and Real-Time PCR
3. Results and Discussion
3.1. Identification of Strains
3.2. Metabolites Analysis
3.3. Genome Assembly and Annotation
3.4. Acetoin Bioproduction Pathway of TYF-LIM-RU47
3.5. Transcriptional Level of Genes Related to Acetoin Metabolite
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Gene Description | Primer Sequence (5′-3′) Forward/Reverse | Product (bp) |
---|---|---|---|
16S rRNA | 16S rRNA | AGAGTTTGATCCTGGCTCAG GGTTACCTTGTTACGACTT | 1500 |
GAPDH | glyceraldehyde-3 phosphate dehydrogenase | GTCGTATCGGCCGTATGGTT CAAAGCTGCTTTGCTACGCT | 293 |
lccA | laccase | GGCTGGCAAGGGACGATTAA TGGTAATGTGAGCGCTTGGT | 280 |
axe | acetylxylan esterase | ACCAACGCTATTCTCGCTCC TGGCCATCTGCGTACACTTT | 206 |
alsS | α-acetolactate synthase | ACAGGTGCTGCTAATGCCAT GCTGTTCTCTTGGCAGGAGT | 299 |
butB | diacetyl reductase | GGCAGGCATTTGTGGAAGTG TCCACCGTCTCCATGCAATC | 271 |
acuABC | acetoin utilization protein acuABC | TGCTTCAGGTGTCGATGCTT TCCGTTGCAGCAACCTCTAG | 166 |
ytrABCDEF | operon related to acetoin transport ytrABCDEF | TAGGGGTCTTAGGGACGGTG TAGTTGCCTTTCGAGCTGGG | 225 |
Substrate | Keep Time (min) | Product Name | Relative Content % |
---|---|---|---|
Corn straw | 6.815 | Ethanol | 4.41 |
7.538 | Acetone | 10.88 | |
10.146 | 2,3-butanedione | 18.12 | |
10.45 | Butanone | 2.93 | |
12.341 | Valeraldehyde | 3.38 | |
12.627 | Ethyl allyl ether | 1.71 | |
15.524 | Acetoin | 53.88 | |
16.077 | Propane | 1.23 | |
22.071 | Propionaldehyde | 0.92 | |
25.19 | Benzaldehyde | 1.51 |
Attribute | Value |
---|---|
GC content (%) | 38.37 |
Scaffolds (>500 bp) | 14 |
Scaffold N50 (bp) | 2,231,473 |
Scaffold N90 (bp) | 188,997 |
Total sequence length (bp) | 3,298,449 |
Gene length (bp) | 2,834,418 |
Total number of genes | 3418 |
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Feng, G.; Fan, X.; Liang, Y.; Li, C.; Xing, J.; He, Y. Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose. Fermentation 2022, 8, 414. https://doi.org/10.3390/fermentation8080414
Feng G, Fan X, Liang Y, Li C, Xing J, He Y. Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose. Fermentation. 2022; 8(8):414. https://doi.org/10.3390/fermentation8080414
Chicago/Turabian StyleFeng, Guoyang, Xiaojun Fan, Yanna Liang, Chen Li, Junde Xing, and Yongji He. 2022. "Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose" Fermentation 8, no. 8: 414. https://doi.org/10.3390/fermentation8080414