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Article

Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract

by
Fatimah A. M. Al-Zahrani
1,*,
Salem S. Salem
2,*,
Huda A. Al-Ghamdi
3,
Laila M. Nhari
3,
Long Lin
4 and
Reda M. El-Shishtawy
5,6,*
1
Chemistry Department, Faculty of Science, King Khalid University, Abha 61413, Saudi Arabia
2
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt
3
Chemistry Department, Faculty of Science, University of Jeddah, Jeddah 21959, Saudi Arabia
4
Colour Science, School of Chemistry, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, UK
5
Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
6
Dyeing, Printing and Textile Auxiliaries Department, Institute of Textile Research and Technology, National Research Centre, 33 EL Buhouth St., Dokki, Giza 12622, Egypt
*
Authors to whom correspondence should be addressed.
Bioengineering 2022, 9(9), 452; https://doi.org/10.3390/bioengineering9090452
Submission received: 20 August 2022 / Revised: 4 September 2022 / Accepted: 5 September 2022 / Published: 8 September 2022
(This article belongs to the Special Issue Biosynthesis of Nanoparticle/Exosome/ECV/Microparticles)
Browse Figures
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Abstract

:
In the study reported in this manuscript, silver/iron oxide nanocomposites (Ag/Fe2O3) were phytosynthesized using the extract of Buddleja lindleyana via a green, economical and eco-friendly strategy. The biosynthesized Ag/Fe2O3 nanocomposites were characterized using UV-Vis spectrophotometry, FTIR, XRD, TEM, DLS and SEM-EDX analyses. The particulates showed a triangular and spherical morphology having sizes between 25 and 174 nm. FTIR studies on the nanoparticles showed functional groups corresponding to organic metabolites, which reduce and stabilize the Ag/Fe2O3 nanocomposite. The antimicrobial efficacy of the phytosynthesized Ag/Fe2O3 against bacterial pathogens was assessed. In addition, Ag/Fe2O3 exhibited broad spectrum activities against B. subtilis, S. aureus, E. coli, and P. aeruginosa with inhibition zones of 23.4 ± 0.75, 22.3 ± 0.57, 20.8 ± 1.6, and 19.5 ± 0.5 mm, respectively. The Ag/Fe2O3 composites obtained showed promising antibacterial action against human bacterial pathogens (S. aureus, E. coli, B. subtilis and P. aeruginosa), making them candidates for medical applications.

Graphical Abstract

1. Introduction

In recent years, leaf extract-mediated biosynthesis of nanomaterials has been extensively studied [1,2,3,4]. Leaf extract-mediated synthesis of nanomaterials is more eco-friendly and economical than other methods of synthesis such as chemical reduction and physical methods [5,6,7]. Due to the growing demand for ecologically friendly material synthesis techniques, the biosynthesis of nanomaterials has gained attention as an emerging feature of the interface of nanotechnology and biotechnology [8,9,10,11,12,13,14,15,16,17]. The biosynthesis of inorganic materials, particularly metal nanoparticles, utilizing microbes and plants has received a lot of attention [18,19,20,21,22,23,24]. Nanosilver has many important applications [25,26,27,28]. Antimicrobial agents have been utilized with it [29,30]. The study reported in the first paper on green leaf cell extract solution-mediated synthesis of Ag/Fe2O3 hybrid nanoparticles successfully employed a green method to synthesize Fe2O3 as well as Ag/Fe2O3 nanoparticles. The synthesized nanoparticles showed excellent antibacterial, antifungal and anticancer activities [31]. Utilizing various reducing agents as well as no reducing agents, it was successful to produce nano-hybrid Ag/Fe2O3NPs depending on carboxymethyl–chitosan. Their fairly reversible magnetization curves show that γ-Fe2O3 NPs transition to a superparamagnetic state [32]. Antibacterial testing on nanocomposites revealed good antimicrobial action against both Gram-positive and Gram-negative bacteria, as well as good activity against yeast and S. aureus resistant strain [33]. The Ag/Fe2O3-Graphene oxide (GO) nanocomposites have shown superior long-term antibacterial capabilities against bacteria (Gram-negative and Gram-positive bacteria), demonstrating their particular potential as a promising long-term biocide with minimal environmental hazard. These abilities were compared to plain Ag nano and Ag/Fe2O3 [34]. The creation of Ag on magnetic-Fe3O4 NPs surfaces modified using Stachys lavandulifolia extract as a reducing and stabilizing magnetic agent was proposed as an effective and environmentally friendly method. Furthermore, the 4-nitrophynol reduction using the nanocomposite and its bactericidal activity was evaluated [35]. Nanocomposites such as Fe3O4 or Ag/Fe2O3 are covered by porous silica oxides serving as magnetic cores to carry anticancer drugs [36]. Nanomaterials which have magnetic behavior, such as Fe3O4 or Ag/ Fe2O3, are good candidates for superparamagnetism [37,38]. Magnetic resonance imagining (MRI) investigations also utilize iron oxide nanoparticles [39]. The combination of Ag and Fe2O3 achieves the properties of both metals [40]. Due to these properties, researchers have been interested in making a hybrid composite of Ag/Fe2O3 offering better catalytic, bactericidal, and bio-imaging properties [41,42,43]. To prepare metal nanoparticles, different techniques such as physicochemical, chemical, and green synthesis by using plant, algae, fungi, and bacterial extracts can be employed [44,45,46,47,48,49,50,51,52,53,54,55]. Plant-based NPs are highly active against different epidemic microbial diseases and are nontoxic to humans [56,57]. The easiest method to synthesize Ag/Fe2O3 nanocomposite by utilizing Algaiia Monozyga extract as a natural reducing and capping agent was studied [41]. Numerous experimenters have utilized Rumix acetosa, Hordeum vulgar, and Azadiracta indica in the green fabrication of Fe2O3NPs [58,59]. Leaf extract-mediated synthesis of Fe2O3NPs is gaining the attention of researchers because of its lower toxicity compared to other chemical reduction methods [28]. Due to their potential utility in targeted drug administration and magnetic hyperthermia cancer treatment, extensive study has been completed on the anticancer properties of Fe2O3NPs [60,61,62,63]. There have been very few reports of investigations on the creation of Ag/Fe2O3 NPs. According to Chen et al., several heteromers of Ag/Fe2O3 NPs may be synthesized and exhibit bactericidal properties [43]. It is hypothesized that a key factor in the antibacterial action of Ag/Fe2O3 NPs is the release of Ag+ ions [41]. Pathogens including bacteria and fungus, which are multi-drug-resistant pathogens, are more easily defeated by Ag/Fe2O3 NPs than by Ag NPs alone. To the best of our knowledge, this is the first publication to examine the biosynthesis of the Ag/Fe2O3 by Buddleja lindleyana leaves. The present study aims to study the green synthesis of nanocomposite Ag/Fe2O3 NPs-based safe natural Buddleja lindleyana leaves as a capping and reducing agent. Additionally, the impact of Ag/Fe2O3 NPs on harmful bacteria was investigated.

2. Materials and methods

2.1. Materials

Buddleja lindleyana leaves were collected in the UK. Fe2 (SO4)3·6H2O and silver nitrate (AgNO3, >99.98%) were purchased from Sigma-Aldrich. All aqueous solutions were prepared using distilled deionized (DI) water.

2.2. Biosynthesis of Ag/Fe2O3

Firstly, Buddleja lindleyana leaves were carefully washed with DI water to remove impurity and then dried for 2 days at room temperature. The Buddleja lindleyana leaves were then weighed out at 10 g and added to 100 mL of DI water. After being heated for 30 min at 90 °C, the mixture was filtered to filter out the broth [31]. The final extract was collected for further usage. For the synthesis of Ag/Fe2O3, a suitable aqueous solution of Buddleja lindleyana extract was gradually added into the mixture containing 1 g Fe(SO4)3·6H2O and 0.1 g silver nitrate (AgNO3) in an Erlenmeyer flask while stirring at 300 rpm with a magnetic stirrer at 70 °C for 3 h.

2.3. Characterization of Ag/Fe2O3

Preliminary characterization of the Ag/Fe2O3 prepared was carried out using UV-Vis spectroscopy. The measurement was carried out using a Jasco dual-beam spectrophotometer (model V-530, Tokyo, Japan) having an operational wavelength range of 300 to 800 nm. Thereafter, the formed pellet was used to identify functional groups present using FTIR spectroscopy. In addition, FTIR was employed to identify the functional groups present in the aqueous leaf extract that led to the formation of nanocomposite. For that, the dry leaf powder of Buddleja lindleyana was pelletized, and their FTIR (Jasco 460 plus, Tokyo, Japan) spectra were recorded between 4000 and 400 cm−1. UV-Vis spectroscopy was used to perform preliminary characterization of the Ag/Fe2O3 produced. With an operating wavelength range of 300 to 800 nm, the measurement was performed using a Jasco dual-beam spectrophotometer (model V-530, Tokyo, Japan). The produced pellet was then utilized to analyze the functional groups using FTIR spectroscopy. Additionally, FTIR was used to pinpoint the functional groups in the aqueous leaf extract that contributed to the creation of the nanocomposite. In order to do that, pellets of Buddleja lindleyana’s dry leaf powder were made, and their FTIR (Jasco, 460-plus, Tokyo, Japan) spectra were taken between 4000 and 400 cm−1. The FTIR spectra for Buddleja lindleyana extract and the spectrum for the aqueous extract of Ag/Fe2O3 were assembled. The size and morphological characterization of the synthesized and lyophilized Ag/Fe2O3 was assessed using transmission electron microscope (TEM). TEM (JEOL-1010, Tokyo, Japan) was used to describe Ag/Fe2O3 in order to determine their sizes and morphologies. The outcome was obtained by injecting the carbon-coated copper grid with Ag/Fe2O3 solution and placing it on a sample holder. Furthermore, the X-ray thin film diffraction measurement for the bio-reduced Ag/Fe2O3 was also performed using a Goniometer = PW3050/60 using Cu kα radiation at 40 kV and 25 °C. Subsequently, relevant X-ray patterns were obtained in the range of 20–80 °C. DLS (dynamic light scattering) particle sizing was employed to study the dispersion of Ag/Fe2O3 in colloidal utilizing the Zetasizer Nanosizer (Malvern-Instruments, Worcestershire, UK). For the particle size analysis, the Ag/Fe2O3 sample was re-suspended in purified water at 25 ppm and vortex-mixed to obtain a homogenous solution. A field emission scanning electron microscope (FESEM) (Quanta, 250-FEG, Taipei, Taiwan) was connected to an energy-dispersive X-ray analyzer (EDX, Unit). EDX and mapping were used to determine the surfaces of the prepared Ag/Fe2O3.

2.4. Assessment of the Inhibitory Activity of Ag/Fe2O3 against Pathogenic Microbes

The inhibitory activity of the Ag/Fe2O3 against bacteria and fungi was assessed using a well-diffusion test. Four bacteria including B. subtilis, S. aureus, E. coli, and P. aeruginosa were employed for the tests. Using the streaking method, test bacteria were inoculated into sterile Petri plates containing 20 mL of Mueller–Hinton. Sterile cork borer (6 mm) was used to prepare wells, and then, 200 µL of Ag/Fe2O3 at a concentration of 5 mg/mL was added in the well. The inoculated plates were kept in a refrigerator for 45 min to achieve adequate diffusion of Ag/Fe2O3, which was followed by incubation at 37 °C for 24 h for the bacteria test. The inhibition zone around each well was measured.

3. Results and Discussion

3.1. Synthesis of Ag/Fe2O3 Nanoparticles

One of the goals of this study was to use Buddleja lindleyana plant sources for the preparation of nanomaterials. The Buddleja lindleyana plant, which belongs to the Loganiaceae family, was chosen and used in this study for Ag/Fe2O3 synthesis. The main chemical components of Buddleja lindleyana include flavonoids, triterpenoids, phenylethaoids, and iridoid glycosides [64,65,66]. The primary idea behind this procedure was to develop a clean, environmentally friendly method of creating nanomaterials using Buddleja lindleyana extract, which has the ability to function as both a reducing and a stabilizing agent in the production of a Ag/Fe2O3 nanocomposite (Figure 1).
The synthesis of Ag/Fe2O3 was conducted by adding Fe(SO4)3·6H2O and AgNO3 as a precursor to the Buddleja lindleyana leaf extract until a gradual change in the reaction color was observed. After 3 h of incubation at 70 °C, the color of the reaction mixture changed from pale green to dark brown (Figure 2). Such a change of color of the reaction mixture represents the formation of Ag/Fe2O3 by the Buddleja lindleyana extract. UV-Vis spectra of the Ag/Fe2O3 nanocomposite after 3 h were measured within the range of 300–800 nm and are presented in Figure 2. The strong peak formed at 320 nm, as visible in the UV-Vis spectra, was used to identify the formation of Ag/Fe2O3 nanocomposite. Similarly, UV-Vis studies of AgFeO2NPs by Berastegui et al. revealed high-level absorptions from 300 to 650 nm [67]. The presence of Ag/Fe2O3 nanocomposite caused the widening of absorbance at 320 nm (Figure 2).
FTIR spectroscopic analysis was used to investigate the structural features of the bioactive constituents in Buddleja lindleyana extract as well as the probable chemical alterations due to the formation of Ag/Fe2O3. As indicated in Figure 3, the bonding arrangements of Buddleja lindleyana were investigated using FTIR in the spectrum range of 400–4000 cm−1 (Figure 3). The H-bonded and -OH stretch vibrating of hydroxyl and phenolic groups of Buddleja lindleyana extract was found in a wide range 3367.9–3216.9 cm−1 [31]. The -CH group is responsible for the bands at 2918.5 cm−1 and 2850.6 cm−1. The presence of C-O of the ester group is indicated by the strong peak seen at 1728.7 cm−1. The existence of NH amine is shown by the peak at 1603.8 cm−1 [20]. The existence of functional groups such as carboxylic acid and ether is further confirmed by FTIR analysis, which provides a peak value of 1008 cm−1. The bioactive chemicals in Buddleja lindleyana extract are employed to convert ions into their appropriate metal forms. Furthermore, these phytochemicals have extremely reactive hydroxyl groups, which give hydrogen and so help to reduce the quantity of free radicals. The findings support the theory that these phytochemicals play a role in the bio-reduction process that leads to the production of nanomaterials [68,69]. According to the IR spectra of Ag/Fe2O3, the suppression of aliphatic molecules in Ag/Fe2O3 can be linked to redox changes of phytochemicals during Ag/Fe2O3 formation (Figure 3). Furthermore, IR studies revealed that chemical groups from the extract were bound to the Ag/Fe2O3 layer, indicating that Buddleja lindleyana extract worked as a stabilizer for the development of nanocomposite. The bending vibration of AgO and FeO interactions in Ag/Fe2O3 may explain the occurrence of peaks at 611.4 and 561.4 cm−1, respectively, in Figure 3.

3.2. Crystalline Structure of Ag/Fe2O3 Nanoparticles

The crystalline structure of the Ag/Fe2O3 NPs prepared was validated using XRD analysis, as shown in Figure 4. The primary strong angles in the diffractogram of the photosynthesized Ag/Fe2O3 were visible in the XRD patterns, showing that the Ag/Fe2O3 nanocomposite was crystallographic in nature. Bands at 31.5°, 35.4°, 38.4°, 42.4°, 44.4°, 52.6°, 57.1°, 64.6°, and 77.6° corresponded to Ag/Fe2O3 diffraction peaks. The values of silver appeared at the angles 38.4°, 44.4°, 64.6°, and 77.6° [20], while the peaks of the angles for iron oxide appeared at 31.5°, 35.4°, 42.4°, 52.6°, and 57.1° [31]. Therefore, the results clearly support the successful synthesis of nano Ag/Fe2O3. Diffraction patterns of the Ag/Fe2O3 nanocomposite did not show the presence of any contaminants, which verifies the purity of the Ag/Fe2O3 nanocomposite obtained.
The most widely used technique for determining the morphological features and sizes of nanostructures is the transmission electron microscope (TEM). Ag/Fe2O3 nanocomposites have been created in various forms such as triangular and spherical with an average size range of 25–174 nm, as seen in the TEM image (Figure 5A). These different forms are a representation of the majority of nanocomposites found in Ag and Fe2O3. The planes of the nanocomposite, as well as the degree of crystallinity of Buddleja lindleyana Ag/Fe2O3 particles, are shown by the bright circular areas in the SAED (selected area electron diffraction) pattern (Figure 5B). The SAED pattern’s ring patterns match the Fe2O3 NPs’ (104), (110), (113), (024), and (122) planes [70]. The SAED pattern further displays planes (100), (200), (220) and (311) that correspond to silver nanoparticles in addition to the aforementioned planes [71]. The average diameter of the Ag/Fe2O3 nanocomposite was determined using dynamic light scattering (DLS) analysis. The produced Ag/Fe2O3 nanocomposites were a poly-dispersed aggregate with an average diameter of 270.9 nm (Figure 5C). However, biomaterials deposited on the Ag/Fe2O3 surface by Buddleja lindleyana, such as organic compounds associated as stabilizers, as well as the metal core (Ag and Fe) of the Ag/Fe2O3 nanocomposite, alter the size determined by DLS [40,72].
SEM was used to analyze the morphology of Ag/Fe2O3 prepared using a simple and single-step process, as demonstrated in Figure 6A. The Ag/Fe2O3 seemed to have a mono-dispersed and aggregation-free micrograph. The EDX spectra revealed the existence of several well-defined peaks in the Ag/Fe2O3 nanocomposite that were due to silver, iron, oxygen, and carbon components (Figure 6B). Furthermore, EDX spectra revealed the production of a very pure Ag/Fe2O3 nanocomposite with no additional impurity-related peaks. The SEM image and the EDX spectra of the nanocomposite prepared revealed that the Ag/Fe2O3 nanostructures were well distributed in the Buddleja lindleyana extract [73]. In addition, elemental EDX mapping was used to determine the Ag and Fe elemental distribution (spatial/lateral) of the Ag/Fe2O3 nanocomposite prepared. The mapping of Fe, Ag, O, and C elements can be seen in the picture (Figure 6C). Both Fe and Ag were uniformly distributed throughout the Ag/Fe2O3 sample, according to the EDX elemental analysis shown in the Ag, Fe, O, and C element mapping images of Ag/Fe2O3 (Figure 6) [41].

3.3. Antimicrobial Activity

Recently, several pathogenic microorganisms have developed resistance to currently available commercial antibiotic agents and also caused adverse impact on human health. Thus, new active and safe antimicrobial agents are required. Because nanomaterials have antibacterial capabilities, they have recently received attention [74,75]. According to Figure 7, the well diffusion technique was used in this investigation to evaluate the Ag/Fe2O3 nanocomposite’s antibacterial efficacy against human bacterial pathogens (S aureus, E. coli, B subtilis, and P. aeruginosa). The maximal Ag/Fe2O3 dose (5 g/mL) showed a high level of inhibitory activity against the pathogens B. subtilis, S. aureus, E. coli, and P. aeruginosa with inhibition zones of 23.4 ± 0.75, 22.3 ± 0.57, 20.8 ± 1.6 and 19.5 ± 0.5 mm, respectively (Figure 7). A previous study showed that silver particles have a good antimicrobial effect on harmful bacteria, whether they are Gram-positive or Gram-negative bacteria [43]. The antibacterial activity of Ag/Fe2O3 nanocomposite was investigated in earlier investigations, and it showed good activity against Gram-negative and Gram-positive bacteria [34,40,76]. Ag/Fe2O3 has an antibacterial impact on bacteria because it damages cell walls, disrupts structural proteins, inactivates enzymes, inhibits electron transport chains, damages nucleic acids (DNA), and causes oxidative stress brought on by the generation of reactive oxygen species (ROS) [41,72,77]. As a result, Ag/Fe2O3 has emerged as a viable antibacterial treatment option and is useful in the medicinal field.

4. Conclusions

Buddleja lindleyana extract was used for the phytosynthesis of Ag/Fe2O3NP through an eco-friendly route. The fabricated Ag/Fe2O3 nanocomposites were characterized using UV-Vis, FTIR, XRD, TEM, DLS and SEM-EDX analyses. The Ag/Fe2O3 nanocomposites obtained are triangular–spheroidal and exhibit a high absorbance band around 320 nm. According to the observation made from the FTIR analysis, it is possible to conclude that the compounds present in Buddleja lindleyana’s extract play an important role in the reduction and stabilization of nanocomposites. The Ag/Fe2O3NPs obtained are stable in colloidal solution. Results also revealed that the Ag/Fe2O3NP nanocomposites fabricated have outstanding antimicrobial activity against pathogenic strains (S aureus, E. coli, B subtilis, and P. aeruginosa). It was found that the antimicrobial activity of the Ag/Fe2O3 nanocomposites is dependent on the structure of the nanocomposites, which have significant activity against all bacterial strains tested. Finally, the Ag/Fe2O3 nanocomposites biosynthesized using the extract of Buddleja lindleyana were also shown to have antibacterial properties, making them promising materials for usage in the medical field.

Author Contributions

Conceptualization, F.A.M.A.-Z. and R.M.E.-S.; methodology, F.A.M.A.-Z., S.S.S. and R.M.E.-S.; software, S.S.S.; validation, L.L.; formal analysis, S.S.S., H.A.A.-G., L.M.N. and L.L.; investigation, F.A.M.A.-Z., S.S.S., H.A.A.-G., L.M.N. and L.L.; resources, L.L.; data curation, H.A.A.-G., L.M.N. and L.L.; writing—original draft preparation, F.A.M.A.-Z. and S.S.S.; writing—review and editing, F.A.M.A.-Z., S.S.S., H.A.A.-G., L.M.N., L.L. and R.M.E.-S.; visualization, R.M.E.-S.; funding acquisition, F.A.M.A.-Z. All authors have read and agreed to the published version of the manuscript.

Funding

Small Groups Project under grant number (RGP.1/252/43) Deanship of Scientific Research at King Khalid University.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data used to support the findings of this study are available from the corresponding author upon request.

Acknowledgments

The authors extend their appreciation to the Scientific Research at King Khalid University for funding this work through Small Groups Project under grant number (RGP.1/252/43).

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. The mechanism of the reduction process of the Ag/Fe2O3 nanocomposite using Buddleja lindleyana extract.
Figure 1. The mechanism of the reduction process of the Ag/Fe2O3 nanocomposite using Buddleja lindleyana extract.
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Figure 2. Color change and UV-Vis absorption spectra of Ag/Fe2O3 nanocomposite synthesis by Buddleja lindleyana extract.
Figure 2. Color change and UV-Vis absorption spectra of Ag/Fe2O3 nanocomposite synthesis by Buddleja lindleyana extract.
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Figure 3. FTIR spectral analysis of Buddleja lindleyana extract and Ag/Fe2O3 nanocomposite.
Figure 3. FTIR spectral analysis of Buddleja lindleyana extract and Ag/Fe2O3 nanocomposite.
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Figure 4. X-ray diffraction (XRD) of the Ag/Fe2O3 synthesized by Buddleja lindleyana extract.
Figure 4. X-ray diffraction (XRD) of the Ag/Fe2O3 synthesized by Buddleja lindleyana extract.
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Figure 5. TEM image (A), SAED pattern (B), and DLS (C) of the synthesized Ag/Fe2O3 by Buddleja lindleyana extract.
Figure 5. TEM image (A), SAED pattern (B), and DLS (C) of the synthesized Ag/Fe2O3 by Buddleja lindleyana extract.
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Figure 6. (A) SEM image, (B) EDX spectrum, and (C) element mapping of Ag/Fe2O3 nanocomposites.
Figure 6. (A) SEM image, (B) EDX spectrum, and (C) element mapping of Ag/Fe2O3 nanocomposites.
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Figure 7. Antimicrobial activity of the synthesized Ag/Fe2O3 by Buddleja lindleyana extract.
Figure 7. Antimicrobial activity of the synthesized Ag/Fe2O3 by Buddleja lindleyana extract.
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Al-Zahrani, F.A.M.; Salem, S.S.; Al-Ghamdi, H.A.; Nhari, L.M.; Lin, L.; El-Shishtawy, R.M. Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract. Bioengineering 2022, 9, 452. https://doi.org/10.3390/bioengineering9090452

AMA Style

Al-Zahrani FAM, Salem SS, Al-Ghamdi HA, Nhari LM, Lin L, El-Shishtawy RM. Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract. Bioengineering. 2022; 9(9):452. https://doi.org/10.3390/bioengineering9090452

Chicago/Turabian Style

Al-Zahrani, Fatimah A. M., Salem S. Salem, Huda A. Al-Ghamdi, Laila M. Nhari, Long Lin, and Reda M. El-Shishtawy. 2022. "Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract" Bioengineering 9, no. 9: 452. https://doi.org/10.3390/bioengineering9090452

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