Engineering Smooth Muscle to Understand Extracellular Matrix Remodeling and Vascular Disease
Abstract
:1. Introduction
2. ECM Properties Affecting SMC Phenotype
2.1. Stiffness
2.2. Fibrillar Protein Composition
2.3. Non-Fibrillar Proteins and Matrix Modifiers
3. Engineering Complex In Vitro Models of Smooth Muscle
3.1. Mechanical Stimulation in Culture Systems
3.2. Organ-on-a-Chip and Multicellular Systems
3.3. Tissue Engineered Vascular Grafts and Pulsatile flow
4. Engineering of Smooth Muscle to Elucidate Mechanisms of Vascular Disease
4.1. Hypertension, Pulmonary Arterial Hypertension, and Atherosclerosis
4.2. Insult and Acute Injury
4.3. Genetic Mutations
5. Conclusions and Future Directions
Funding
Conflicts of Interest
References
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Extracellular Matrix (ECM) Property | Factor or Pathway of Interest | Experimental Model | Findings |
---|---|---|---|
Stiffness | Lysyl oxidase-like 2 (LOXL2) | Human smooth muscle cells (SMCs) in 2D culture; LOXL2 knockout mice |
|
Mineralocorticoid receptor (MR) | MR deleted male mice |
| |
Circulating molecules | Parabiosis with young and old mice |
| |
Focal adhesion kinases (FAKs) and N-cadherin | FAK knockout mice |
| |
FAK and N-cadherin knockout mice |
| ||
Transforming factor beta (TGF-β) signaling pathway | Human SMCs on collagen I (COL1)-coated polyacrylamide (PA) gels |
| |
Human SMCs on polymethylsiloxane (PDMS) |
| ||
Human SMCs on silk fibroin gels |
| ||
DNA methyltransferase I (DNMT1) | Human SMCs on fibronectin (FN)-coated PA gels; acute aortic injury and chronic kidney failure mouse models |
| |
Ascending thoracic aortic aneurysm | Healthy and aneurysmal human SMCs on compliant hydrogels |
| |
Fibrillar Protein Composition | Fibronectin | Porcine SMCs suspended in COL1-FN gels |
|
Elastin | Human smooth muscle cells on porous collagen-elastin scaffold sheets |
| |
Collagen 1 and fibronectin | Human smooth muscle cells on ECM-coated polyacrylamide gels |
| |
Non-fibrillar Protein Abundance and Structure | Advanced Glycation End products (AGEs) | Mice models |
|
Hyaluronic acid (HA) | Human SMCs cultured on micropatterned and HA/ECM-coated titanium |
| |
Rho-related BTB domain–containing protein 1 (RhoBTB1) | Angiotensin-II treated (hypertensive) mice |
| |
Elastin assembly proteins | Knockout mice models |
| |
Small leucine-rich repeat proteoglycans | Human coronary artery bypass patients |
| |
Lysyl hydroxylase I (PLOD1), lysyl oxidase (LOX) | Human and mouse SMCs cultured in osteogenic medium |
| |
Post-translationally modified (glycosylated) fibronectin (gFN) | Rat SMCs on fibronectin |
| |
Protein and lipid phosphatase (PTEN) | Mice with SMC-specific-PTEN knockout; Isolated human atherosclerotic arteries |
| |
Matrix metalloproteinase-12 (MMP12) | MMP12 knockout mice |
| |
Matrixmetalloproteinase-9 (MMP9) | Macrophage depleted mice |
|
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Yarbrough, D.; Gerecht, S. Engineering Smooth Muscle to Understand Extracellular Matrix Remodeling and Vascular Disease. Bioengineering 2022, 9, 449. https://doi.org/10.3390/bioengineering9090449
Yarbrough D, Gerecht S. Engineering Smooth Muscle to Understand Extracellular Matrix Remodeling and Vascular Disease. Bioengineering. 2022; 9(9):449. https://doi.org/10.3390/bioengineering9090449
Chicago/Turabian StyleYarbrough, Danielle, and Sharon Gerecht. 2022. "Engineering Smooth Muscle to Understand Extracellular Matrix Remodeling and Vascular Disease" Bioengineering 9, no. 9: 449. https://doi.org/10.3390/bioengineering9090449