Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Film Fabrication
2.3. ssDNA Immobilization and Hybridization
2.4. X-ray Photoelectron Spectroscopy
2.5. Electrochemistry
3. Results and Discussions
3.1. XPS
3.2. Electrochemical Studies
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Relative Capacitance | Charge Transfer Resistance |
---|---|---|
Au | 100% | 25 Ω |
Au/PEG | 67% | 41 Ω |
Au/PEG+A10 | 65% | 40 Ω |
Au/PEG+T10 | 65% | 43 Ω |
Au/PEG/NHS-T5 | 50% | 95 Ω |
Au/PEG/NHS-T5+T5 | 49% | 97 Ω |
Au/PEG/NHS-T5+A5 | 32% | 195 Ω |
Au/PEG/NHS-T10 | 34% | 158 Ω |
Au/PEG/NHS-T10+T10 | 33% | 155 Ω |
Au/PEG/NHS-T10+A10 | 19% | 330 Ω |
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Zhao, Z.; Das, S.; Zharnikov, M. Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization. Bioengineering 2022, 9, 414. https://doi.org/10.3390/bioengineering9090414
Zhao Z, Das S, Zharnikov M. Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization. Bioengineering. 2022; 9(9):414. https://doi.org/10.3390/bioengineering9090414
Chicago/Turabian StyleZhao, Zhiyong, Saunak Das, and Michael Zharnikov. 2022. "Rational Design of Porous Poly(ethylene glycol) Films as a Matrix for ssDNA Immobilization and Hybridization" Bioengineering 9, no. 9: 414. https://doi.org/10.3390/bioengineering9090414