1. Introduction
Bread is one of the most frequently consumed foods, being the main source of energy supplied for the body [
1], but refined wheat flour, which is the key ingredient for the manufacture of bread and bakery products, has a reduced nutritional value due to its lower contents of fiber, vitamins, minerals, and other compounds compared to whole wheat flour [
2]. Numerous studies in the specialized literature highlight the improvement of the nutritional characteristics of wheat flour through the incorporation of new functional ingredients and the development of safe and healthy nutritional products [
3,
4,
5]. In this sense, bakery products enriched with dietary fiber, amino acids, and bioactive compounds from flours obtained from whole grains or pseudo-cereals, which prevent the diseases associated with the metabolic syndrome, such as cardiovascular diseases, arteriosclerosis, and colon cancer, are an alternative [
6].
Interest in pseudocereals’ use in food technologies has increased in recent years, because they offer a valuable nutritional composition, having a high-quality protein content amino acid composition point of view [
7]. Amaranth is a pseudocereal recognized for its main functional components, namely dietary fiber (11.10%) [
8], proteins (10.18–29.35%) [
9], polyunsaturated fatty acids (3.23%) [
10], vitamins (riboflavin: 0.19–0.23%), ascorbic acid (4.50%), folic acid and vitamin E [
11], minerals (calcium: 178 mg/100 g; magnesium: 248 mg/100 g; phosphorus: 557 mg/100 g; potassium: 508 mg/100 g) [
10], and various bioactive compounds [
12]. The biggest limitation in the functional properties is that amaranth does not contain gluten and therefore has no dough-forming or baking properties. Due to the lack of gluten in amaranth, its addition to flour, especially in large quantities, in wheat flour dough results in a modification of the processing conditions and the quality of the final product [
11,
13,
14]. Up to a certain amount, amaranth flour can be added to wheat-based products, thereby improving the nutritional properties of the resulting product. Depending on the amount of wheat flour substituted (12–50%), several groups of researchers have reported an improvement of the final product compared to wheat flour bread in terms of protein (from 14.29% to 16.30%), lipids (from 0.67% to 1.75%), fiber, and minerals (in particular, Cu: from 2.25 to 4.21 µg/g, Mn: from 6.39 to 19.41 µg/g, Zn: from 11.65 to 24.95 µg/g, Fe: from 18.85 to 43.74 µg/g, Ca: from 0.31 to 0.99 mg/g, Mg: from 0.29 to 1.32 mg/g, and K: from 1.88 to 3.21 mg/g) [
13,
14,
15]. Improved bakery products can be formulated by adding amaranth flour to refined wheat flour, at different addition doses and particle sizes, with specific functional properties. The functionality and chemical composition of flours are influenced by their particle size distribution. Fractions of flour with different particle sizes sieved from the same flour result in different chemical compositions [
9], which will affect the wheat–amaranth flour’s composition [
16]. The incorporation of amaranth flour in bread-making may influence dough rheological properties and consequently the baking performance of bread [
17,
18]. Rosales-Juárez et al. [
19] demonstrated that specific bread volumes, bread colors, textural properties, and flavors are correlated with WF quality and the breadmaking processes. For example, the gluten in wheat flour is vital for the viscoelastic properties and carbon dioxide retention capacity of bread dough during fermentation and the initial stages of baking [
20,
21] and consequently determines bread volume [
22] and textural properties of breadcrumbs [
23].
The use of protein-rich and gluten-free amaranth flour mixed with cereal flour had a negative impact on the dough’s rheological properties. The addition of amaranth flour at different doses (10, 20, and 30%) in refined wheat flour or whole wheat flour determined an increase in dough tenacity and a decrease in its extensibility [
24]. In another study [
25], it was found that bread enriched with expanded amaranth in concentrations of 10, 15 and 20% had a higher content of minerals (Zn, Cu, Fe, Mn, Na, K, Mg, and Ca), proteins (13.90%), dietary fiber (1.11%), and squalene (43 mg/100 g), but the bread’s specific volume decreased by up to 33%. The addition of
Amaranthus cruentus whole meal in gradual doses up to 40% to wheat flour remarkably increased the protein, lipid, ash, and dietary fiber contents of white bread [
13]. An increase in minerals such as Cu, Mn, Zn, Fe, Ca, Mg, and P was also found, while the hardness of the crumb was influenced only by the sample containing 40% amaranth, and the structure of the crumb did not show significant changes. An improvement of the nutritional value of the product by increasing the content of dietary fiber, minerals, and proteins was reported when whole wheat flour was substituted with amaranth at 50% [
26]. Although, amaranth bread did not achieve higher acceptability than wheat bread, especially the one with a high percentage of substitution (40%), consumers concluded that the preference for amaranth bread is based on its nutritional value, even if the taste and flavor are different from wheat bread.
Enhancing nutritional aspects when using wheat–amaranth composite flour with different particle sizes of amaranth in bread-making requires an adapted formulation. A desirability function has previously been applied to find the optimal amount of AF added in WF, depending on the AF particle size, to enhance dough and bread properties [
27]. These optimal doses consider the baking characteristics of the flour, and bread quality parameters were established based on predictive models obtained for twenty-four parameters, as shown in a previous study [
27], by applying the analysis of multiple responses. It was highlighted that AF of large, medium, and small particle size added to WF at different doses is crucial for obtaining the desired rheological properties (determined by using empirical and dynamic rheological tests) and a suitable bread volume and textural firmness. However, there are no studies that report the effects of optimal wheat–amaranth composite flour depending on different amaranth flour particle sizes in terms of the physical properties, degree of liking (consumer acceptability), and nutritional profile of the resulting bread. Therefore, the aim of this study was to evaluate the chemical, physical, textural, and sensory properties of bread baked from optimal wheat–amaranth composite flour specific to large, medium, and small amaranth flour particle size.
3. Results
3.1. Nutritional Characteristics of Wheat Flour and Amaranth Flour Fractions
The physicochemical characterization of the composition of wheat flour and amaranth flour fractions was reported in a previous study [
27]. In addition, in this work, the mineral and amino-acid contents were determined, and the results were presented in the
Supplementary Materials (
Table S1 and
Figure S1).
3.2. The Baking Characteristics of the Optimal Wheat–Amaranth Composite Flour and the Quality of the Bread
The baking characteristics of the optimal wheat–amaranth formulated composite flours and the quality parameters of the bread compared to wheat dough and bread are shown in
Table 1.
According to the obtained results, the optimal wheat–amaranth composite flours with large fractions presented α-amylase activity, quantified by the falling number (FN) index, similar to that of the control sample, whereas the optimal composite flour with medium and small fraction presented a slightly lower α-amylase activity.
Dough stability (ST), protein thermal weakening (C1-2), and starch gelatinization (C3-2) also showed values very close to those of the control sample, while the dough development time (DT) and the stability of the hot gel (C3-4) were considerably higher than the control sample, whilst starch retrogradation (C5-4) presented significantly higher values for the optimal composite flour than for wheat flour.
Alveographic parameters’ values presented differences between samples, the highest values of dough tenacity being observed for the optimal dough with small AF particle size, while the lowest extensibilty value was obtained for the optimal composite dough with a large particle size.
The optimal dough samples with large, medium, and small particle sizes had a higher maximum height (H’m), volume of gas total (VT) and retained (VR), and gas retention capacity (CR) compared to wheat flour dough. The elasticity (G′) and viscosity (G″) modulus showed higher values than the control for all of the optimal dough samples, which indicated the viscoelastic nature of the dough, the results being expected. Remarkable differences were obtained between the optimal wheat–amaranth composite flour dough samples with large fraction sizes (O_AL) and those with medium and small sizes (O_AM and O_AS). The maximum gelatinization temperature (Tmax) decreased compared to the control sample. A lower resistance to deformation indicated by an increase in the creep compliance (Jcmax) value was obtained for the samples with the optimal addition of small sizes of amaranth flour compared to the control sample.
Regarding bread volume, an increase was observed in the optimal composite flour bread with medium particle size, being similar to that of the wheat flour bread, while the optimal composite bread with large and small particle sizes presented lower values for bread volume.
3.3. Advanced Characterization of the Bread Obtained from Optimal Wheat–Amaranth Composite Flour for Each Amaranth Flour Particle Size Studied
3.3.1. The Physical Characteristics of Optimal Bread
According to the experimental data, significant differences were obtained between the specific volume, porosity, and elasticity of bread samples with the optimal addition levels of amaranth flour fractions (
Table 2).
The optimized bread with the small fraction showed a lower specific volume than the control sample. Regarding the porosity and elasticity of the bread crumb with the optimal dose of amaranth flours, the best result was obtained for the large fraction.
Colour is an essential attribute of food products, and it influences consumer perception and food product acceptability. Flour was one of the factors that determined bread samples’ color. The colour parameters—the lightness (
L*), the intensity of the red (
a*) or green (−
a*), and the yellow (
b*) or blue (−
b*) shade—for the crust and the crumb of the breads made from the optimal composite flours varied according to the related optimal dose (
Table 3).
In the case of bread samples with the optimal dose of amaranth fractions, the crust lightness decreased in the following order: O_AL < O_AS < O_AM. The values of the crust bread a varied in the positive range for bread samples for optimal composite flour and in the negative range for the control sample, and the highest value was obtained for bread from the optimum flour composite with a medium particle size of the amaranth flour.
3.3.2. Texture Parameters of the Optimal Breads
The textural parameters of bread have a direct influence on the consumer’s perception and choice, and the physical parameters of the bread samples obtained with the optimal dose of AF addition corresponding to the large (L), medium (M), and small (S) particle sizes compared to bread from refined wheat flour are presented in
Table 4.
According to the experimental data obtained from the texture analysis of the bread samples, significant differences (p < 0.05) were observed between the firmness of bread crumb with AF fractions compared to the control. A gradual increase in firmness was observed in bread with particle size reduction, while the bread sample with large AF particles showed lower firmness values. The elasticity of the bread samples with the optimal dose of amaranth decreased in the following order: M < L < S, with significant differences between the optimal samples and the control bread, but no significant differences were recorded between the samples with medium and small particles.
3.3.3. Nutritional Composition and Energy Value of Breads with Amaranth Flour at the Optimal Addition Dose for Each Studied Particle Size
The physico-chemical properties of the bread samples made from the optimal composite flours for each AF compared to the control bread are shown in
Table 5.
The chemical composition of the optimized bread samples, corresponding to each amaranth flour particle size, showed significant differences (
p < 0.05) between the samples and compared to the control sample. The results indicated an improvement in the protein, lipid, and ash contents of bread obtained from the optimal composite flour, especially in the case of large and medium fractions of amaranth flour, while the carbohydrate content and energy values decreased (
Table 5).
3.3.4. Macro- and Micro-Elements Content of the Optimal Bread Samples
The composition in macro- and micro-elements of the bread samples obtained from the optimal composite flours was influenced by the particle size composition of the amaranth flour which partially replaced refined wheat flour (
Figure 1). An increase in the content of macro- and micro-elements was observed depending on the particle size as follows: O_AM > O_AS > O_AL. The optimal bread samples obtained from wheat–amaranth composite flour presented a significantly higher macro and mineral content. The higher values of K (356 mg/100 g), Ca (105 mg/100 g), and Mg (59.7 mg/100 g) were found in the optimal bread with a medium amaranth flour fraction, while no differences in sodium content were found in the optimal composite bread samples, in contrast to the wheat flour bread where identified K (126 mg/100 g), Ca (23 mg/100 g), and Mg (13.7 mg/100 g) were identified. The optimal composite wheat–amaranth medium fraction bread contains 2.20 mg/100 g Zn, 3.00 mg/100 g Fe, 1.13 mg/100 g Mn, and 0.623 mg/100 g Cu, values that are significantly higher than those present in wheat flour bread (0.94, 1.54, 0.62, and 0.55, respectively). The mineral content of the optimal bread samples increased significantly as a result of wheat flour replacement, as expected due to the amaranth flour particle size composition.
3.3.5. Determining the Amino Acid Content of Optimal Bread Samples
The variation of the essential and non-essential amino acid (AA) content in bread samples with the optimal dose (O) of amaranth flour addition corresponding to large (L), medium (M), and small (S) particle sizes and in comparison with bread made from refined wheat flour is presented in
Figure 2.
Six essential amino acids were identified in bread samples formulated with the optimal doses of amaranth flour, for each individual particle size, (
Figure 2a), representing 16.16% for the bread with the large fraction, 15.3% for the bread with the medium fraction, and 13.48% for the bread with the small fraction of amaranth flour, from the total AA profile. The amino acid content for the bread samples formulated with the optimal doses of AF fractions varied as follows: isoleucine (4.41–6.35%), leucine (4.33–6.39%), methionine (7.62–8.94%), phenylalanine (8.9–10.48%), threonine (9.30–9.25%), valine (4.89–10.30%), alanine (5.60–8.53%), aspartic acid (18.12–29.45%), glutamic acid (4.97–81.47%), glutamine (109.00–115.24%), glycine (3.20–12.03%), serine (7.32–8.06%), tyrosine (9.25–10.25%), asparagine (6.43–7.64%), proline (7.05–8.15%), thioproline (12.37–20.38%), and hydroxyproline (9.06–13.25%). An increase in AA content was observed in bread with the optimal dose of medium particles, followed by the bread with small-size particles and a lower content than in the control sample was identified in bread obtained with the optimal dose of AF large particles.
3.3.6. Sensory Analysis of Breads Obtained from Optimal Composite Flours
The results of the sensory analysis in terms of overall acceptability, appearance, crumb structure, taste, and smell for the optimal bread formulations compared to the control can be observed in
Figure 3. From the average scores of the sensory characteristics evaluated in this study, it can be observed that the optimal bread with medium fractions was preferred by the panel. The optimal bread with large and medium fractions obtained the highest scores for overall acceptability, appearance, crumb structure, taste, and smell. Significant differences were observed between these two optimal bread samples and wheat flour bread.
3.4. Evaluating Relationships between Variables
Relationships between the variables were highlighted by applying a multivariate technique, principal components analysis (PCA). The application of PCA provided information about the similarities and/or differences between the evaluated variables, in terms of chemical composition, dough dynamic rheological parameters, bread physical, texture, and sensory properties (
Figure 4).
From the analysis of Pearson correlation matrix, direct or indirect correlations were observed, significant at p < 0.05, between some of the determined characteristics.
4. Discussion
Considering the impact of the nutritional characteristics of wheat flour and amaranth flour fractions on the final product, the results of the mineral and amino-acids contents are discussed in relation to the nutritional properties of bread obtained from optimal wheat–amaranth composite flour specific to each particle size and wheat bread. Alencar et al. (2015) [
35] found that adding 20% amaranth flour to a mixture containing rice flour, potato starch, cassava starch, and sour tapioca starch enhanced the nutritional value of bread due to the high levels of protein, fat, and minerals and a bread specific volume and firmness similar to control bread.
The increase in the FN index, of the optimal formulations with medium and small AF particle sizes, leads to a decrease in α-amylase activity, which can be correlated with the presence of calcium ions in amaranth grains [
36]. Dough development time was correlated with dough strength and was significantly higher in the optimal composite flour, while dough stability didn’t present a difference compared with wheat flour. Starch retrogradation of the optimal composite flour showed significantly lower values than wheat flour, which can indicate that amaranth flour addition can limit starch retrogradation, increasing bread freshness and shelf-life. The biaxial extension of dough indicated a significant increase in the dough tenacity of the optimal composite sample, associated with lower extensibility. Fermentation properties were significantly improved for all of the optimal composite doughs, compared to the wheat flour dough. Dynamic rheological behavior indicated strong interactions between starch and gluten due to the high values of the G′ and G″ moduli. Moreover, the complex bonds that can be formed between starch granules and amaranth flour fibers can also determine a raise of the moduli. The variation of the viscosity factor (tan δ) for the optimal dough samples indicated a decrease e for the samples with medium and small particle sizes of amaranth flour (O_AL and O_AM), but all the optimal formulations showed values close to those of the control sample. This difference in viscosity factor values may be explained by the high protein content present in the amaranth flour fractions [
27]. The maximum gelatinization temperature (T
max) of optimal wheat–amaranth composite flour decreased in all of the optimal wheat–amaranth composite flours, a fact that may be due to the insoluble amylose–lipid complexes formation that occurred during the heating of starch slurries, which reduces and delays the swelling of starch granules [
24]. Creep-recovery compliance is affected by the protein, carbohydrate, or starch content of amaranth flours. The hydroxyl groups will interact with proteins groups and will lead to non-covalent or covalent bonding [
28]. The differences between the optimal formulations and the control samples in terms of empirical and dynamic rheological properties, as well as the volume and firmness of bread, can be explained by their contents of proteins, lipids, carbohydrates [
27], minerals (
Table S1), amino acids (
Figure S1), and other compounds present in the amaranth flour fractions.
Bread obtained from optimal composite flour determined a slight decrease in volume. This behavior can be explained by the intake of dietary fiber from the large fractions, which interact with wheat glutenin through disulfide bonds, not diluting the gluten network very much [
37]. The best results regarding bread porosity and elasticity were obtained when the large fraction was incorporated, this fact being possible due to the high content of starch and lipids from these fractions, which contribute as a stabilizing agent to the stabilization of the cells gases [
38].
Regarding bread textural properties, an increase of crumb firmness in bread formulations was observed when AF particle size decreased. This fact can be explained by the dietary fiber content of the large particle, which contains albumin that can interact with wheat glutenin through disulfide bonds and will act as a surface active agent and thus will maintain the gluten matrix [
37,
39]. Similar results were obtained for breadspringiness, this phenomenon can be related to the high lipid content of the medium fraction of amaranth flour [
27], as it is known that polar lipids can act as a gas stabilizer during the bread-making process, leading to an improvement in bread elasticity [
38].
Generally, both colour of the crust and of the bread crumb impact consumer acceptability. The colour of the optimal bread formulations varied depending on the fraction used in optimal bread, this fact being possible due to the intense Maillard-type reaction that occurs in the samples with the addition of protein-rich fractions. In addition, the decrease in colour lightness of the optimal bread may also be explained by the higher amount of phenolic and flavonoids compounds of the AF [
40,
41]
The increase in the nutritional value of bread may be due to the high content of amaranth flour fractions in these nutrients [
9], and the different proteins, lipids and ash localization in the seed. Data from the specialized literature show that amaranth seed proteins are located in the germ of the seed (65%) [
42]. This variation is due to the different chemical compositions of the central endosperm, aleurone layer, embryo, and cell wall tissues in amaranth seeds, as explained by Steadman et al. (2001) [
43].
The results obtained were consistent with those reported by Sanz-Penella et al. [
13] and Miranda-Ramos et al. [
44]. Bread with the addition of large, medium, and small particle sizes of AF at certain/optimal doses in wheat flour was enriched nutritionally, from a mineral point of view (
Figure 4), compared to wheat bread. In general, white bread is low in minerals and could be supplemented to meet the daily requirements for different mineral elements [
45,
46], amaranth flour presenting high potential in this sense. Potassium (K) and calcium (Ca) were found in higher amounts in medium particles, while magnesium (Mg) and sodium (Na) were found in higher amount in small particles. For the total content of macro and micro elements, medium particles presented the highest content. For the small size fraction of amaranth flour, which consists mainly of the endosperm part of the seeds, a large amount of nutritionally important elements was found (
Table S1). Minerals such as phosphorus, potassium, and magnesium are located in the embryo, while calcium is present in the pericarp and has been associated with those pectic compounds of the cell wall [
47]. The cellular material in the ground endosperm can contribute significantly to the ash content [
48]. Many of the minerals were identified in much higher concentrations than in other grains [
49]. Considerable differences were observed between the optimal bread formulations and also between them and the wheat bread in terms of the determined minerals, but a high content of K, Ca, Mg, Zn, and Fe was found in the optimal samples compared to the control. The optimal bread with AF medium particle size presented the highest mineral content being followed by the bread with small particle size, results which were related to the minerals content from these fractions.
Miranda-Ramos et al. [
44] replaced wheat flour with amaranth flour in doses of 25 and 50% and reported higher Ca, Fe, and Zn values for the formulated bread than those obtained in this research. Although Kumar et al. [
50] studied the mineral content of whole AF and three fractions, to our knowledge, no other study has been conducted on the mineral content of bread with different AF particle sizes.
The bread made from optimal wheat–amaranth composite flour was enhanced with essential and nonessential amino acids compared to wheat flour bread. Methionine, phenylalanine, and threonine were the essential amino acids found in higher amounts in the optimal bread for all amaranth fractions. The variation in the amino acids content can be explained due to the presence of the AA from the amaranth fractions (
Figure S1) that are present especially in the bran, which creates an intercellular skeleton that prevents the action of digestive enzymes and thus reduces the content of amino acids in the seed coat [
51]. Asparagine was not found, because it was completely converted to aspartic acid under acid hydrolysis conditions [
52]. Aspartic and glutamic acids were present in larger quantities in all of the optimal bread formulations, glutamic acid being present in larger quantities than in the control bread only in the optimal bread with small size fractions (O_AS).
One of the main challenges in wheat flour bread production replaced by non-gluten amaranth flour is the level of consumer acceptance. This is crucial considering that the appearance, aroma, taste, and texture of food play a key role in the acquisition process. Compared with the control sample, an improvement of the organoleptic characteristics of the optimal bread formulations, excepting optimal bread with AF small particle size, was found. The optimal bread with medium and large particle sizes presented similar or better sensorial characteristics than that of the control bread. The respondent’s acceptance can be explained by the soft crumbs due to the presence of natural emulsifiers in amaranth flours. Our results are in agreement with those found by other authors when they made bread from amaranth flour [
53].
The use of principal component analysis (PCA) highlighted a plot of the features and type of the sample on the two principal components (
Figure 4). The first two main components (PC1 and PC2) explained 86.05% of the total variance of the analyzed variables, where PC1 explained 61.70%. PC1 was associated with the viscoelastic moduli and the maximum creep and recovery compliance of dough samples as well as with the moisture, protein, lipid, and carbohydrate contents of optimal breads and the firmness, gumminess, porosity, appearance, crumb structure, taste, and smell of bread samples. PC2 was associated with the viscosity factor and the maximum gelatinization temperature of the dough, ash content, springiness, cohesiveness, and overall acceptability of bread. A strong association was observed between the optimal composite flour with large PS and the lipid, protein, and porosity of the bread. The optimal sample with medium particles of amaranth flour was associated with viscoelastic moduli and bread taste, while the optimal sample with small-PS amaranth flour was related to the maximum creep and recovery compliance of the dough and with the bread volume and firmness. By applying the Pearson correlation analysis between the evaluated characteristics, a series of very strong correlations were obtained (0.95 < r < 0.99), significant at a significance level of
p < 0.05. The protein content of bread was directly correlated with the viscosity modulus (r = 0.95) and inversely with the gumminess and chewiness of the bread (r = −0.97). Positive correlations were obtained between the carbohydrate content of bread and the gumminess (r = 0.99) and chewiness (r = 0.99), while these texture parameters are negatively associated with the viscosity modulus (r = −0.98; r = −0.99). Regarding bread firmness, an inverse correlation was found with the viscosity factor (r = −0.97), while bread elasticity was directly associated with the maximum gelatinization temperature (r = 0.98). Bread cohesiveness was negatively associated with bread crumb porosity (r = −0.99). The appearance of the bread was negatively correlated with the bread volume (r = −0.96), while the taste of the bread was positively correlated with the viscous modulus (r = 0.98). Some authors found a direct correlation between dough elasticity and bread firmness and chewiness [
54,
55].
5. Conclusions
The bread produced from optimal wheat–amaranth composite flour specific to each particle size presented an improvement in volume, porosity, and elasticity, while the crust and crumb lightness, firmness, resilience, and chewiness decreased in bread with small particle sizes compared to wheat-flour bread. From a nutritional point of view, bread made with amaranth flour at an established addition dose depending on particle size showed a significant increase in protein, lipid, and ash content, with higher content of potassium, calcium, magnesium, zinc, and iron compared to the wheat flour bread values, while the carbohydrate content and energy value decreased. The highest protein content was found in bread with medium and small particle sizes, an increase of 26.7%, and 15.8%, respectively, being observed. There was an increase in the mineral content by over 37% in the bread samples obtained with composite flour with medium and small particles, and an improvement in protein by more than 26% in the optimal bread with large particles compared to wheat flour bread. Phenylalanine, threonine, and valine were the essential amino acids, while aspartic and glutamic acids were the nonessential amino acids that were identified in higher amounts in the studied samples. An increase in the total amino acid content of 19.5% and 8.7% was observed in the optimal bread with small and medium amaranth flour, respectively. The results of the sensory analysis revealed an increase in the overall acceptability score, appearance, crumb structure, taste, and smell for the bread samples obtained from the optimal composite flours containing medium and large particles of amaranth flour. The bread made from composite flour with medium particles was very well accepted with a score higher than 8.5 for the general acceptability.
As a result, it can be concluded that the optimal composite bread with AF medium particle size presents the highest nutritional, physical, and sensorial values, followed by the optimal composite bread made with AF small particle size.