Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sarcopenic Obesity Induction and Experimental Design
2.2. Muscle Function Test
2.3. Cognitive Function Test
2.4. Body Composition Analysis
2.5. Histological Assays
2.6. Western Blot Analysis
2.7. Statistical Analysis
3. Results
3.1. Induction of Sarcopenic Obesity
3.2. Cognitive and Muscle Function and Their Correlation
3.3. Morphological Analysis of Hippocampus
3.4. Cognitive Function-Related Myokines and Energy Metabolism in Skeletal Muscle
3.5. Cognitive Function-Related Myokines, Energy Metabolism, and Oxidative stress and Inflammation in Hippocampus
3.6. Cognitive Function-Related Myokines in Plasma
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Batsis, J.A.; Villareal, D.T. Sarcopenic Obesity in Older Adults: Aetiology, Epidemiology and Treatment Strategies. Nat. Rev. Endocrinol. 2018, 14, 513–537. [Google Scholar] [CrossRef] [PubMed]
- Tolea, M.; Chrisphonte, S.; Galvin, J. Sarcopenic Obesity and Cognitive Performance. Clin. Interv. Aging 2018, 13, 1111–1119. [Google Scholar] [CrossRef] [Green Version]
- Gariballa, S.; Alessa, A. Association between Muscle Function, Cognitive State, Depression Symptoms and Quality of Life of Older People: Evidence from Clinical Practice. Aging Clin. Exp. Res. 2018, 30, 351–357. [Google Scholar] [CrossRef] [PubMed]
- Peng, T.-C.; Chen, W.-L.; Wu, L.-W.; Chang, Y.-W.; Kao, T.-W. Sarcopenia and Cognitive Impairment: A Systematic Review and Meta-Analysis. Clin. Nutr. 2020, 39, 2695–2701. [Google Scholar] [CrossRef] [PubMed]
- Dye, L.; Boyle, N.B.; Champ, C.; Lawton, C. The Relationship between Obesity and Cognitive Health and Decline. Proc. Nutr. Soc. 2017, 76, 443–454. [Google Scholar] [CrossRef] [Green Version]
- Tabassum, S.; Misrani, A.; Yang, L. Exploiting Common Aspects of Obesity and Alzheimer’s Disease. Front. Hum. Neurosci. 2020, 14, 602360. [Google Scholar] [CrossRef] [PubMed]
- Pratchayasakul, W.; Sa-nguanmoo, P.; Sivasinprasasn, S.; Pintana, H.; Tawinvisan, R.; Sripetchwandee, J.; Kumfu, S.; Chattipakorn, N.; Chattipakorn, S.C. Obesity Accelerates Cognitive Decline by Aggravating Mitochondrial Dysfunction, Insulin Resistance and Synaptic Dysfunction under Estrogen-Deprived Conditions. Horm Behav. 2015, 72, 68–77. [Google Scholar] [CrossRef]
- Lizarbe, B.; Cherix, A.; Duarte, J.M.N.; Cardinaux, J.-R.; Gruetter, R. High-Fat Diet Consumption Alters Energy Metabolism in the Mouse Hypothalamus. Int. J. Obes. 2019, 43, 1295–1304. [Google Scholar] [CrossRef]
- Tan, B.L.; Norhaizan, M.E. Effect of High-Fat Diets on Oxidative Stress, Cellular Inflammatory Response and Cognitive Function. Nutrients 2019, 11, 2579. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Scisciola, L.; Fontanella, R.A.; Surina; Cataldo, V.; Paolisso, G.; Barbieri, M. Sarcopenia and Cognitive Function: Role of Myokines in Muscle Brain Cross-Talk. Life 2021, 11, 173. [Google Scholar] [CrossRef]
- Filardi, M.; Barone, R.; Bramato, G.; Nigro, S.; Tafuri, B.; Frisullo, M.E.; Zecca, C.; Tortelli, R.; Logroscino, G. The Relationship Between Muscle Strength and Cognitive Performance Across Alzheimer’s Disease Clinical Continuum. Front. Neurol. 2022, 13, 833087. [Google Scholar] [CrossRef]
- Jodeiri Farshbaf, M.; Alviña, K. Multiple Roles in Neuroprotection for the Exercise Derived Myokine Irisin. Front. Aging Neurosci. 2021, 13, 649929. [Google Scholar] [CrossRef]
- Isaac, A.R.; Lima-Filho, R.A.S.; Lourenco, M.V. How Does the Skeletal Muscle Communicate with the Brain in Health and Disease? Neuropharmacology 2021, 197, 108744. [Google Scholar] [CrossRef]
- Severinsen, M.C.K.; Pedersen, B.K. Muscle–Organ Crosstalk: The Emerging Roles of Myokines. Endocr. Rev. 2020, 41, 594–609. [Google Scholar] [CrossRef]
- Barbalho, S.M.; Flato, U.A.P.; Tofano, R.J.; de Goulart, R.A.; Guiguer, E.L.; Detregiachi, C.R.P.; Buchaim, D.V.; Araújo, A.C.; Buchaim, R.L.; Reina, F.T.R.; et al. Physical Exercise and Myokines: Relationships with Sarcopenia and Cardiovascular Complications. Int. J. Mol. Sci. 2020, 21, 3607. [Google Scholar] [CrossRef]
- Peterson, M.D.; Rhea, M.R.; Sen, A.; Gordon, P.M. Resistance Exercise for Muscular Strength in Older Adults: A Meta-Analysis. Ageing Res. Rev. 2010, 9, 226–237. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Gadelha, A.B.; Paiva, F.M.L.; Gauche, R.; de Oliveira, R.J.; Lima, R.M. Effects of Resistance Training on Sarcopenic Obesity Index in Older Women: A Randomized Controlled Trial. Arch. Gerontol. Geriatr. 2016, 65, 168–173. [Google Scholar] [CrossRef] [PubMed]
- Chiu, S.-C.; Yang, R.-S.; Yang, R.-J.; Chang, S.-F. Effects of Resistance Training on Body Composition and Functional Capacity among Sarcopenic Obese Residents in Long-Term Care Facilities: A Preliminary Study. BMC Geriatr. 2018, 18, 21. [Google Scholar] [CrossRef] [Green Version]
- Yoon, D.H.; Lee, J.-Y.; Song, W. Effects of Resistance Exercise Training on Cognitive Function and Physical Performance in Cognitive Frailty: A Randomized Controlled Trial. J. Nutr. Health Aging 2018, 22, 944–951. [Google Scholar] [CrossRef] [PubMed]
- Babaei, P.; Azari, H.B. Exercise Training Improves Memory Performance in Older Adults: A Narrative Review of Evidence and Possible Mechanisms. Front. Hum. Neurosci. 2022, 15, 771553. [Google Scholar] [CrossRef]
- Sandrini, L.; di Minno, A.; Amadio, P.; Ieraci, A.; Tremoli, E.; Barbieri, S. Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis. Int. J. Mol. Sci. 2018, 19, 2281. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Miranda, M.; Morici, J.F.; Zanoni, M.B.; Bekinschtein, P. Brain-Derived Neurotrophic Factor: A Key Molecule for Memory in the Healthy and the Pathological Brain. Front. Cell Neurosci. 2019, 13, 363. [Google Scholar] [CrossRef]
- Tu, W.-J.; Qiu, H.-C.; Cao, J.-L.; Liu, Q.; Zeng, X.-W.; Zhao, J.-Z. Decreased Concentration of Irisin Is Associated with Poor Functional Outcome in Ischemic Stroke. Neurotherapeutics 2018, 15, 1158–1167. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chen, H.-T.; Chung, Y.-C.; Chen, Y.-J.; Ho, S.-Y.; Wu, H.-J. Effects of Different Types of Exercise on Body Composition, Muscle Strength, and IGF-1 in the Elderly with Sarcopenic Obesity. J. Am. Geriatr Soc. 2017, 65, 827–832. [Google Scholar] [CrossRef] [PubMed]
- Pedersen, B.K. Physical Activity and Muscle–Brain Crosstalk. Nat. Rev. Endocrinol. 2019, 15, 383–392. [Google Scholar] [CrossRef] [PubMed]
- Lee, H.; Lim, Y. The Potential Role of Myokines/Hepatokines in the Progression of Neuronal Damage in Streptozotocin and High-Fat Diet-Induced Type 2 Diabetes Mellitus Mice. Biomedicines 2022, 10, 1521. [Google Scholar] [CrossRef] [PubMed]
- Wheeler, M.J.; Green, D.J.; Ellis, K.A.; Cerin, E.; Heinonen, I.; Naylor, L.H.; Larsen, R.; Wennberg, P.; Boraxbekk, C.-J.; Lewis, J.; et al. Distinct Effects of Acute Exercise and Breaks in Sitting on Working Memory and Executive Function in Older Adults: A Three-Arm, Randomised Cross-over Trial to Evaluate the Effects of Exercise with and without Breaks in Sitting on Cognition. Br. J. Sports Med. 2020, 54, 776–781. [Google Scholar] [CrossRef] [PubMed]
- Guo, A.; Li, K.; Xiao, Q. Sarcopenic Obesity: Myokines as Potential Diagnostic Biomarkers and Therapeutic Targets? Exp. Gerontol. 2020, 139, 111022. [Google Scholar] [CrossRef]
- Alizadeh Pahlavani, H. Exercise Therapy for People With Sarcopenic Obesity: Myokines and Adipokines as Effective Actors. Front. Endocrinol. 2022, 13, 811751. [Google Scholar] [CrossRef] [PubMed]
- Shin, J.-E.; Jeon, S.-H.; Lee, S.-J.; Choung, S.-Y. The Administration of Panax Ginseng Berry Extract Attenuates High-Fat-Diet-Induced Sarcopenic Obesity in C57BL/6 Mice. Nutrients 2022, 14, 1747. [Google Scholar] [CrossRef]
- Jin, H.; Oh, H.-J.; Nah, S.-Y.; Lee, B.-Y. Gintonin-Enriched Fraction Protects against Sarcopenic Obesity by Promoting Energy Expenditure and Attenuating Skeletal Muscle Atrophy in High-Fat Diet-Fed Mice. J. Ginseng. Res. 2022, 46, 454–463. [Google Scholar] [CrossRef] [PubMed]
- Effting, P.S.; Brescianini, S.M.S.; Sorato, H.R.; Fernandes, B.B.; dos Fidelis, G.S.P.; da Silva, P.R.L.; Silveira, P.C.L.; Nesi, R.T.; Ceddia, R.B.; Pinho, R.A. Resistance Exercise Modulates Oxidative Stress Parameters and TNF-a Content in the Heart of Mice with Diet-Induced Obesity. Arq. Bras. Cardiol. 2019, 112, 545–552. [Google Scholar] [PubMed]
- Zhai, L.; Liu, Y.; Zhao, W.; Chen, Q.; Guo, T.; Wei, W.; Luo, Z.; Huang, Y.; Ma, C.; Huang, F.; et al. Aerobic and Resistance Training Enhances Endothelial Progenitor Cell Function via Upregulation of Caveolin-1 in Mice with Type 2 Diabetes. Stem Cell Res. Ther. 2020, 11, 10. [Google Scholar] [CrossRef] [Green Version]
- Ueno, H.; Takahashi, Y.; Murakami, S.; Wani, K.; Miyazaki, T.; Matsumoto, Y.; Okamoto, M.; Ishihara, T. The Prevention of Home-Cage Grid Climbing Affects Muscle Strength in Mice. Sci. Rep. 2022, 12, 15263. [Google Scholar] [CrossRef]
- Zeraati, M.; Najdi, N.; Mosaferi, B.; Salari, A.-A. Environmental Enrichment Alters Neurobehavioral Development Following Maternal Immune Activation in Mice Offspring with Epilepsy. Behav. Brain Res. 2021, 399, 112998. [Google Scholar] [CrossRef] [PubMed]
- van Praag, H. Exercise Enhances Learning and Hippocampal Neurogenesis in Aged Mice. J. Neurosci. 2005, 25, 8680–8685. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hamdan, A.M.; Al-Gayyar, M.M.; Shams, M.E.E.; Alshaman, U.S.; Prabahar, K.; Bagalagel, A.; Diri, R.; Noor, A.O.; Almasri, D. Thymoquinone Therapy Remediates Elevated Brain Tissue Inflammatory Mediators Induced by Chronic Administration of Food Preservatives. Sci. Rep. 2019, 9, 7026. [Google Scholar] [CrossRef] [Green Version]
- Laurens, C.; Bergouignan, A.; Moro, C. Exercise-Released Myokines in the Control of Energy Metabolism. Front. Physiol. 2020, 11, 91. [Google Scholar] [CrossRef]
- Kumar, S.; Hossain, J.; Inge, T.; Balagopal, P.B. Changes in Myokines in Youths With Severe Obesity Following Roux-En-Y Gastric Bypass Surgery. JAMA Surg. 2019, 154, 668. [Google Scholar] [CrossRef]
- Mancinelli, R.; Checcaglini, F.; Coscia, F.; Gigliotti, P.; Fulle, S.; Fanò-Illic, G. Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging. Int. J. Mol. Sci. 2021, 22, 8520. [Google Scholar] [CrossRef]
- Gaitán, J.M.; Moon, H.Y.; Stremlau, M.; Dubal, D.B.; Cook, D.B.; Okonkwo, O.C.; van Praag, H. Effects of Aerobic Exercise Training on Systemic Biomarkers and Cognition in Late Middle-Aged Adults at Risk for Alzheimer’s Disease. Front. Endocrinol. 2021, 12, 660181. [Google Scholar] [CrossRef]
- So, B.; Kim, H.-J.; Kim, J.; Song, W. Exercise-Induced Myokines in Health and Metabolic Diseases. Integr. Med. Res. 2014, 3, 172–179. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hsu, K.-J.; Liao, C.-D.; Tsai, M.-W.; Chen, C.-N. Effects of Exercise and Nutritional Intervention on Body Composition, Metabolic Health, and Physical Performance in Adults with Sarcopenic Obesity: A Meta-Analysis. Nutrients 2019, 11, 2163. [Google Scholar] [CrossRef] [Green Version]
- Kobilo, T.; Liu, Q.-R.; Gandhi, K.; Mughal, M.; Shaham, Y.; van Praag, H. Running Is the Neurogenic and Neurotrophic Stimulus in Environmental Enrichment. Learn. Mem. 2011, 18, 605–609. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wrann, C.D.; White, J.P.; Salogiannnis, J.; Laznik-Bogoslavski, D.; Wu, J.; Ma, D.; Lin, J.D.; Greenberg, M.E.; Spiegelman, B.M. Exercise Induces Hippocampal BDNF through a PGC-1α/FNDC5 Pathway. Cell Metab. 2013, 18, 649–659. [Google Scholar] [CrossRef] [Green Version]
- Lin, J.-Y.; Kuo, W.-W.; Baskaran, R.; Kuo, C.-H.; Chen, Y.-A.; Chen, W.S.-T.; Ho, T.-J.; Day, C.H.; Mahalakshmi, B.; Huang, C.-Y. Swimming Exercise Stimulates IGF1/ PI3K/Akt and AMPK/SIRT1/PGC1α Survival Signaling to Suppress Apoptosis and Inflammation in Aging Hippocampus. Aging 2020, 12, 6852–6864. [Google Scholar] [CrossRef]
- Liu, P.Z.; Nusslock, R. Exercise-Mediated Neurogenesis in the Hippocampus via BDNF. Front. Neurosci. 2018, 12, 52. [Google Scholar] [CrossRef] [Green Version]
- Numakawa, T.; Odaka, H. Brain-Derived Neurotrophic Factor and Neurogenesis. In Factors Affecting Neurodevelopment; Elsevier: Amsterdam, The Netherlands, 2021; pp. 121–131. [Google Scholar]
- Jin, Y.; Sun, L.H.; Yang, W.; Cui, R.J.; Xu, S.B. The Role of BDNF in the Neuroimmune Axis Regulation of Mood Disorders. Front. Neurol. 2019, 10, 515. [Google Scholar] [CrossRef] [Green Version]
- Whiteman, E.L.; Cho, H.; Birnbaum, M.J. Role of Akt/Protein Kinase B in Metabolism. Trends Endocrinol. Metab. 2002, 13, 444–451. [Google Scholar] [CrossRef]
- Jiao, P.; Feng, B.; Li, Y.; He, Q.; Xu, H. Hepatic ERK Activity Plays a Role in Energy Metabolism. Mol. Cell. Endocrinol. 2013, 375, 157–166. [Google Scholar] [CrossRef]
- Boyer, J.G.; Prasad, V.; Song, T.; Lee, D.; Fu, X.; Grimes, K.M.; Sargent, M.A.; Sadayappan, S.; Molkentin, J.D. ERK1/2 Signaling Induces Skeletal Muscle Slow Fiber-Type Switching and Reduces Muscular Dystrophy Disease Severity. JCI Insight 2019, 4, e127356. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Garcia, S.; Nissanka, N.; Mareco, E.A.; Rossi, S.; Peralta, S.; Diaz, F.; Rotundo, R.L.; Carvalho, R.F.; Moraes, C.T. Overexpression of PGC-1α in Aging Muscle Enhances a Subset of Young-like Molecular Patterns. Aging Cell 2018, 17, e12707. [Google Scholar] [CrossRef]
- Moon, H.Y.; Becke, A.; Berron, D.; Becker, B.; Sah, N.; Benoni, G.; Janke, E.; Lubejko, S.T.; Greig, N.H.; Mattison, J.A.; et al. Running-Induced Systemic Cathepsin B Secretion Is Associated with Memory Function. Cell Metab. 2016, 24, 332–340. [Google Scholar] [CrossRef] [Green Version]
- Roh, E.; Choi, K.M. Health Consequences of Sarcopenic Obesity: A Narrative Review. Front. Endocrinol. 2020, 11, 332. [Google Scholar] [CrossRef]
- Valcarcel-Ares, M.N.; Tucsek, Z.; Kiss, T.; Giles, C.B.; Tarantini, S.; Yabluchanskiy, A.; Balasubramanian, P.; Gautam, T.; Galvan, V.; Ballabh, P.; et al. Obesity in Aging Exacerbates Neuroinflammation, Dysregulating Synaptic Function-Related Genes and Altering Eicosanoid Synthesis in the Mouse Hippocampus: Potential Role in Impaired Synaptic Plasticity and Cognitive Decline. J. Gerontol. Ser. A 2019, 74, 290–298. [Google Scholar] [CrossRef]
- Liu, Y.; Chu, J.M.T.; Yan, T.; Zhang, Y.; Chen, Y.; Chang, R.C.C.; Wong, G.T.C. Short-Term Resistance Exercise Inhibits Neuroinflammation and Attenuates Neuropathological Changes in 3xTg Alzheimer’s Disease Mice. J. Neuroinflamm. 2020, 17, 4. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ge, X.; Xu, X.; Feng, C.; Wang, Y.; Li, Y.; Feng, B. Relationships among Serum C-Reactive Protein, Receptor for Advanced Glycation Products, Metabolic Dysfunction, and Cognitive Impairments. BMC Neurol. 2013, 13, 110. [Google Scholar] [CrossRef] [Green Version]
- Ekdahl, C.T.; Claasen, J.-H.; Bonde, S.; Kokaia, Z.; Lindvall, O. Inflammation Is Detrimental for Neurogenesis in Adult Brain. Proc. Natl. Acad. Sci. USA 2003, 100, 13632–13637. [Google Scholar] [CrossRef] [Green Version]
- Yin, F.; Sancheti, H.; Patil, I.; Cadenas, E. Energy Metabolism and Inflammation in Brain Aging and Alzheimer’s Disease. Free Radic. Biol. Med. 2016, 100, 108–122. [Google Scholar] [CrossRef] [Green Version]
- Junnila, R.K.; List, E.O.; Berryman, D.E.; Murrey, J.W.; Kopchick, J.J. The GH/IGF-1 Axis in Ageing and Longevity. Nat. Rev. Endocrinol. 2013, 9, 366–376. [Google Scholar] [CrossRef] [PubMed]
- Belviranlı, M.; Okudan, N. Exercise Training Increases Cardiac, Hepatic and Circulating Levels of Brain-Derived Neurotrophic Factor and Irisin in Young and Aged Rats. Horm Mol. Biol. Clin. Investig. 2018, 36. [Google Scholar] [CrossRef] [PubMed]
- Huh, J.Y.; Panagiotou, G.; Mougios, V.; Brinkoetter, M.; Vamvini, M.T.; Schneider, B.E.; Mantzoros, C.S. FNDC5 and Irisin in Humans: I. Predictors of Circulating Concentrations in Serum and Plasma and II. MRNA Expression and Circulating Concentrations in Response to Weight Loss and Exercise. Metabolism 2012, 61, 1725–1738. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jin Regulation of BDNF-TrkB Signaling and Potential Therapeutic Strategies for Parkinson’s Disease. J. Clin. Med. 2020, 9, 257. [CrossRef] [PubMed] [Green Version]
- Polyakova, M.; Schroeter, M.L.; Elzinga, B.M.; Holiga, S.; Schoenknecht, P.; de Kloet, E.R.; Molendijk, M.L. Brain-Derived Neurotrophic Factor and Antidepressive Effect of Electroconvulsive Therapy: Systematic Review and Meta-Analyses of the Preclinical and Clinical Literature. PLoS ONE 2015, 10, e0141564. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fahimi, A.; Baktir, M.A.; Moghadam, S.; Mojabi, F.S.; Sumanth, K.; McNerney, M.W.; Ponnusamy, R.; Salehi, A. Physical Exercise Induces Structural Alterations in the Hippocampal Astrocytes: Exploring the Role of BDNF-TrkB Signaling. Brain Struct. Funct. 2017, 222, 1797–1808. [Google Scholar] [CrossRef] [PubMed]
Group | CON | OB | EX | |
---|---|---|---|---|
Body Weight (g) | ||||
Before Exercise | 31.33 ± 2.39 a | 39.39 ± 3.93 b | 40.14 ± 4.63 b | |
After Exercise | 31.70 ± 2.63 a | 43.64 ± 5.27 b | 42.01 ± 5.41 b | |
Difference | 0.38 ± 1.08 a | 4.25 ± 2.03 c | 1.87 ± 1.63 b | |
Body Composition (% of Total Mass) | ||||
Before Exercise | Fat Mass | 22.54 ± 2.94 a | 35.26 ± 6.28 b | 36.58 ± 5.41 b |
Lean Mass | 74.93 ± 2.87 b | 62.60 ± 6.12 a | 61.35 ± 5.31 a | |
After Exercise | Fat Mass | 24.77 ± 3.55 a | 38.34 ± 7.43 b | 38.90 ± 5.54 b |
Lean Mass | 72.58 ± 3.47 b | 59.53 ± 7.24 a | 58.91 ± 5.45 a | |
Difference | Fat Mass | 2.26 ± 2.50 | 3.09 ± 2.80 | 2.32 ± 1.76 |
Lean Mass | −2.35 ± 2.46 | −3.08 ± 2.71 | −2.45 ± 1.77 |
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Lim, G.; Lee, H.; Lim, Y. Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice. Biomedicines 2022, 10, 2529. https://doi.org/10.3390/biomedicines10102529
Lim G, Lee H, Lim Y. Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice. Biomedicines. 2022; 10(10):2529. https://doi.org/10.3390/biomedicines10102529
Chicago/Turabian StyleLim, Gahyun, Heaji Lee, and Yunsook Lim. 2022. "Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice" Biomedicines 10, no. 10: 2529. https://doi.org/10.3390/biomedicines10102529