Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Standards and Reagents
2.2. Plant Extracts and Essential Oils
2.3. Fungal Organisms
2.4. Susceptibility Tests
2.5. Germ Tube and Dimorphic Transition Inhibition Assay
2.6. Statistical Analysis
3. Results
3.1. Antifungal Susceptibility Tests
3.2. Germ Tube and Dimorphic Transition Inhibition Assay
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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C. albicans | C. albicans H37 (FR) | C. glabrata | C. krusei | C. parapsilosis | C. tropicalis | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
MIC a | MLC b | MIC a | MLC b | MIC a | MLC b | MIC a | MLC b | MIC a | MLC b | MIC a | MLC b | |
C. chinensis (D) | 5.00 | 10.00 | 5.00 | 10.00 | 5.00 | >20.00 * | 0.63 | 0.63 | 2.5 | 2.5 | 0.63 | 1.25 |
C. chinensis (EO) | 5.00 | 10.00 | 5.00 | 10.00 | 5.00 | >20.00 * | 0.63 | 0.63 | 2.5 | 2.5 | 0.63 | 1.25 |
L.scoparium (EO) | 20.00 | >20.00 | 10.00 | 20.00 | 10.00 | >20.00 | 20.00 | ≥20.00 | ≥20.00 | >20.00 | >20.00 | >20.00 |
M. officinalis (D) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
M. officinalis (EO) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
A. indica (D) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
A. indica (EO) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
S. barbata (D) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
S. barbata (EO) | >20.00 | - | - | - | >20.00 | - | >20.00 | - | >20.00 | - | >20.00 | - |
Concentration µL/mL | C. chinensis Decoction | C. chinensis EO | L. scoparium EO |
---|---|---|---|
Control | 84.6 ± 8.5 | 84.6 ± 8.5 | 84.6 ± 8.5 |
10 | 64.1 ± 4.1 (2MIC) | 59.3 ± 5.7 (2MIC) | 0.0 ± 0.0 (MIC/2) |
5 | 88.2 ± 3.9 (MIC) | 89.4 ± 4.9 (MIC) | 5.1 ± 1.2 (MIC/4) |
2.5 | 89.4 ± 6.4 (MIC/2) | 93.5 ± 3.9 (MIC/2) | 8.3 ± 2.5 (MIC/8) |
1.25 | - | - | 10.0 ± 6.2 (MIC/16) |
0.63 | - | - | 12.0 ± 5.3 (MIC/32) |
0.31 | - | - | 40.7 ± 8.3 (MIC/64) |
0.16 | - | - | 76.0 ± 10.5 (MIC/128) |
0.08 | - | - | 92.3 ± 4.9 (MIC/256) |
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Noites, A.; Araújo, B.; Machado, J.; Pinto, E. Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species. Healthcare 2022, 10, 1820. https://doi.org/10.3390/healthcare10101820
Noites A, Araújo B, Machado J, Pinto E. Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species. Healthcare. 2022; 10(10):1820. https://doi.org/10.3390/healthcare10101820
Chicago/Turabian StyleNoites, Alexandra, Bruno Araújo, Jorge Machado, and Eugénia Pinto. 2022. "Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species" Healthcare 10, no. 10: 1820. https://doi.org/10.3390/healthcare10101820