FastQCFastQC Report
Wed 13 Feb 2019
CerA21_forward_paired.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCerA21_forward_paired.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21167042
Sequences flagged as poor quality0
Sequence length50-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG5550.024.3029161
CGCCCTT50400.014.648751
CGCGTAT13350.012.7622651
CTCGCTA57900.012.3833619
CGGGCAT60900.012.0065361
CGCCCAT59950.011.3678891
AGATCGG148850.011.096845140-144
GGAAAAT510950.011.0038471
GCCGGGT33600.010.9865631
CGGGCAC35450.010.613471
CGGGCCT27750.09.9769871
CGGGTAT41400.09.9454721
TCGCTAG42050.09.7916919
GCCCGAT41400.09.7739981
CCCGGCT30300.09.6059211
GCGGGCT19500.09.4653451
CGCGCAT18800.09.4401721
CGCGGTA18551.8189894E-129.1847031
CGCGGGC7753.9750384E-49.1600121
CGCGGAT20250.09.1147781
Produced by FastQC (version 0.11.5)