The Effect of Glycine Betaine on Nitrogen and Polyamine Metabolisms, Expression of Glycoside-Related Biosynthetic Enzymes, and K/Na Balance of Stevia under Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparing Samples and Applying Treatments
2.2. Growth Parameters and Photosynthetic Pigments
2.3. Free Polyamines
2.4. Methionine and Arginine
2.5. Hydrogen Peroxide and Malondialdehyde
2.6. Relative Water Content and Membrane Stability Index
2.7. Activity of Polyamine Catabolizing Enzymes
2.8. Activity of Antioxidant Enzymes
2.9. Nitrogen and Nitrate Content
2.10. Activity of Nitrogen Metabolism Enzymes
2.11. Sodium and Potassium Concentration
2.12. Diterpene Glycosides
2.13. Gene Expression
2.14. Statistical Analyses
3. Results
3.1. Growth and Photosynthesis Traits
3.2. Metabolism of Polyamines
3.3. Oxidative Stress
3.4. K/Na Homeostasis
3.5. Nitrogen Metabolism
3.6. Diterpene Glycosides
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments (mM) | N Node | N Root | TDW | Chl a | Chl b | Car |
---|---|---|---|---|---|---|
mg/gFW | ||||||
Control | 8.10 ± 0.26 a | 7.55 ± 0.20 b | 0.251 ± 0.022 a | 1.91 ± 0.15 a | 1.10 ± 0.08 a | 0.348 ± 0.023 a |
GB 25 | 8.39 ± 0.17 a | 8.08 ± 0.24 a | 0.275 ± 0.021 a | 2.03 ± 0.16 a | 1.08 ± 0.07 a | 0.354 ± 0.015 a |
GB 50 | 8.24 ± 0.13 a | 7.45 ± 0.21 bc | 0.251 ± 0.016 a | 2.05 ± 0.10 a | 1.04 ± 0.10 ab | 0.342 ± 0.024 a |
NaCl 50 | 3.66 ± 0.18 d | 4.88 ± 0.22 e | 0.121 ± 0.009 d | 1.02 ± 0.07 d | 0.64 ± 0.03 c | 0.207 ± 0.018 d |
NaCl 50 + GB 25 | 5.29 ± 0.15 c | 6.73 ± 0.23 d | 0.179 ± 0.011 c | 1.47 ± 0.08 c | 0.92 ± 0.04 b | 0.263 ± 0.016 c |
NaCl 50 + GB 50 | 6.24 ± 0.20 b | 7.10 ± 0.23 cd | 0.209 ± 0.009 b | 1.69 ± 0.09 b | 0.99 ± 0.04 ab | 0.299 ± 0.016 b |
Treatments (mM) | K | Na | K/Na | N (mg/gDW) | NO3 (µmol/gfw) |
---|---|---|---|---|---|
mg/gDW | |||||
Control | 4.81 ± 0.30 ab | 0.72 ± 0.04 d | 6.70 ± 0.16 a | 33.07 ± 0.60 a | 28.17 ± 0.72 a |
GB 25 | 4.81 ± 0.30 ab | 0.73 ± 0.03 d | 6.56 ± 0.12 a | 32.26 ± 0.55 a | 26.96 ± 1.20 a |
GB 50 | 4.90 ± 0.27 a | 0.72 ± 0.04 d | 6.82 ± 0.05 a | 32.44 ± 0.79 a | 27.39 ± 0.79 a |
NaCl 50 | 2.99 ± 0.15 d | 2.46 ± 0.10 a | 1.22 ± 0.02 d | 17.87 ± 0.60 d | 13.70 ± 0.44 d |
NaCl 50 + GB 25 | 3.96 ± 0.16 c | 1.30 ± 0.06 b | 3.06 ± 0.26 c | 25.33 ± 0.43 c | 20.91 ± 0.63 c |
NaCl 50 + GB 50 | 4.39 ± 0.14 b | 1.02 ± 0.10 c | 4.36 ± 0.53 b | 28.25 ± 0.43 b | 22.96 ± 0.64 b |
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Ghorbani, A.; Ghasemi-Omran, V.O.; Chen, M. The Effect of Glycine Betaine on Nitrogen and Polyamine Metabolisms, Expression of Glycoside-Related Biosynthetic Enzymes, and K/Na Balance of Stevia under Salt Stress. Plants 2023, 12, 1628. https://doi.org/10.3390/plants12081628
Ghorbani A, Ghasemi-Omran VO, Chen M. The Effect of Glycine Betaine on Nitrogen and Polyamine Metabolisms, Expression of Glycoside-Related Biosynthetic Enzymes, and K/Na Balance of Stevia under Salt Stress. Plants. 2023; 12(8):1628. https://doi.org/10.3390/plants12081628
Chicago/Turabian StyleGhorbani, Abazar, Vali Ollah Ghasemi-Omran, and Moxian Chen. 2023. "The Effect of Glycine Betaine on Nitrogen and Polyamine Metabolisms, Expression of Glycoside-Related Biosynthetic Enzymes, and K/Na Balance of Stevia under Salt Stress" Plants 12, no. 8: 1628. https://doi.org/10.3390/plants12081628