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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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G. Świerczek-Zięba
J. Lodowska
U. Mazurek
S. Kurkiewicz
T. Wilczok
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
Author to whom correspondence should be addressed.
Sci. Pharm. 2001, 69(2), 167-178;
Submission received: 2 May 2000 / Accepted: 16 January 2001 / Published: 30 June 2001


It is quite well known that β-blockers influence the stability of cell membranes, but the effect of metoprolol on the composition of cell membrane lipids is not established. On the other hand, synchronous culture of Chlorella vulgaris cells, which consists of cells brought to the same developmental stage by cycling lighting, provides a convenient biological model in unidirectional analyses aimed at assessment of effects of xenobiotics on cells. Advantages of the model include short life cycle and possibility to control metabolic processes of the cells across the broad range. We assessed the effect of metoprolol on fatty acids composition of cell membrane phospholipids in consecutive life cycle stages of Chlorella vulgaris. Lipids were extracted using a chlorofonn/methanol method. Phospholipids were precipitated from the chloroform phase with cold acetone and following centrifugation the pellet of phospholipids was hydrolyzed in alkaline solution. Fatty acids were extracted with petroleum ether and then methylated with BF3-methanol. This protocol produced methyl esters of fatty acids which were subjected to GC-MS analysis. Metoprolol had no effect on the number of progeny cells of Chlorella vulgaris throughout their life cycle at the concentration range tested (5x10-5 M, 5x10-6 M, 5x10-7 M). However, we observed stimulation of biological activity of Chlorclla cells as measured by spectrophotomctry at λ=680 nm. Metoprolol, at the highest concentration, increased phospholipids content in mother cells. Simultaneously, relative amount and saturation level of thc fatty acids remained constant.
Keywords: fatty acids; phospholipids; biotest; metoprolol; Chlorella vulgaris fatty acids; phospholipids; biotest; metoprolol; Chlorella vulgaris

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MDPI and ACS Style

Świerczek-Zięba, G.; Lodowska, J.; Mazurek, U.; Kurkiewicz, S.; Wilczok, T. METOPROLOL EFFECT ON FATTY ACIDS COMPOSITION OF CELL MEMBRANE PHOSPHOLIPIDS. Sci. Pharm. 2001, 69, 167-178.

AMA Style

Świerczek-Zięba G, Lodowska J, Mazurek U, Kurkiewicz S, Wilczok T. METOPROLOL EFFECT ON FATTY ACIDS COMPOSITION OF CELL MEMBRANE PHOSPHOLIPIDS. Scientia Pharmaceutica. 2001; 69(2):167-178.

Chicago/Turabian Style

Świerczek-Zięba, G., J. Lodowska, U. Mazurek, S. Kurkiewicz, and T. Wilczok. 2001. "METOPROLOL EFFECT ON FATTY ACIDS COMPOSITION OF CELL MEMBRANE PHOSPHOLIPIDS" Scientia Pharmaceutica 69, no. 2: 167-178.

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