Virulomic Analysis of Multidrug-Resistant Klebsiella pneumoniae Isolates and Experimental Virulence Model Using Danio rerio (Zebrafish)
Abstract
:1. Introduction
2. Results
2.1. WGS, Resistance and Virulence Genes
2.2. Zebrafish Virulence Model Preliminary Phase: Determination of Inoculum
2.3. Zebrafish Virulence Model
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Whole-Genome Sequencing, and Genetic Mapping
4.2. Selection of Strains for the In Vivo Experimental Model
4.3. Zebrafish: Inoculum Determination and Virulence Model
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | MLST | Siderophore | Regulation | Allantoin Metabolism | Auto Transporter | Efflux Pump AcrAB | Stress Adaptation |
---|---|---|---|---|---|---|---|
“A” | 23 | Aerobactin, Yersiniabactin | rcsA, rcsB | Yes | No | Yes | Yes |
“B” | 11 | Enterobactin, Yersiniabactin | rcsA, rcsB | No | cah | Yes | Yes |
“C” | 11 | Enterobactin, Yersiniabactin | rcsA, rcsB | No | cah | Yes | Yes |
“D” | 16 | None | rcsA, rcsB | No | No | Yes | No |
“E” | 16 | None | rcsA, rcsB | No | No | Yes | No |
“F” | 16 | Enterobactin, Yersiniabactin | rcsA, rcsB | No | No | Yes | No |
Group ID | ST | Injected Embryos | Alive 24 hpi | PMR | Alive 48 hpi | PMR | Alive 72 hpi | PMR | Alive 96 hpi | Endpoint Mortality |
---|---|---|---|---|---|---|---|---|---|---|
“A” | 23 | 31 | 22 | 29% | 22 | 29% | 22 | 29% | 20 | 35% |
“B” | 11 | 31 | 27 | 13% | 24 | 22% | 24 | 22% | 22 | 29% |
“C” | 11 | 33 | 28 | 15% | 24 | 27% | 24 | 27% | 23 | 30% |
“D” | 16 | 37 | 30 | 19% | 22 | 40% | 22 | 40% | 20 | 46% |
“E” | 16 | 35 | 27 | 23% | 20 | 43% | 18 | 48% | 18 | 48% |
“F” | 16 | 31 | 26 | 16% | 18 | 42% | 16 | 48% | 11 | 64% |
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Duarte, E.L.T.; Rizek, C.F.; Espinoza, E.S.; Marchi, A.P.; Noguera, S.V.; Côrtes, M.F.; Fernandes, B.H.V.; Guimarães, T.; de Maio Carrilho, C.M.D.; Neto, L.V.P.; et al. Virulomic Analysis of Multidrug-Resistant Klebsiella pneumoniae Isolates and Experimental Virulence Model Using Danio rerio (Zebrafish). Antibiotics 2022, 11, 1567. https://doi.org/10.3390/antibiotics11111567
Duarte ELT, Rizek CF, Espinoza ES, Marchi AP, Noguera SV, Côrtes MF, Fernandes BHV, Guimarães T, de Maio Carrilho CMD, Neto LVP, et al. Virulomic Analysis of Multidrug-Resistant Klebsiella pneumoniae Isolates and Experimental Virulence Model Using Danio rerio (Zebrafish). Antibiotics. 2022; 11(11):1567. https://doi.org/10.3390/antibiotics11111567
Chicago/Turabian StyleDuarte, Edson Luiz Tarsia, Camila Fonseca Rizek, Evelyn Sanchez Espinoza, Ana Paula Marchi, Saidy Vasconez Noguera, Marina Farrel Côrtes, Bianca H. Ventura Fernandes, Thais Guimarães, Claudia M. D. de Maio Carrilho, Lauro V. Perdigão Neto, and et al. 2022. "Virulomic Analysis of Multidrug-Resistant Klebsiella pneumoniae Isolates and Experimental Virulence Model Using Danio rerio (Zebrafish)" Antibiotics 11, no. 11: 1567. https://doi.org/10.3390/antibiotics11111567