Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals, Reagents, and Apparatus
2.2. Fabrication and Features of Different Paper Electrode Setups
2.3. Construction of Dengue Aptasensor to Check the Current Range of Both Electrode Setups
3. Results
3.1. Comparative Study of Current Amplification Based on Three- and Two-Electrode Setup, and Summarizing Their Differences in the Currents-
3.1.1. Current Comparison of Different Stages of Aptasensor
3.1.2. Current Comparison of Different Concentrations of Dengue Antigen
3.2. Justification for Using Three-Electrode System over Two-Electrode System
4. Conclusions and Future Perspective
5. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Santhiago, M.; Henry, C.S.; Kubota, L.T. Low cost simple three dimensional electrochemical paper-based analytical device for determination of p-nitrophenol. Electrochim. Acta 2017, 130, 771–777. [Google Scholar] [CrossRef]
- Anzar, N.; Hasan, R.; Tyagi, M.; Yadav, N.; Narang, J. Carbon nanotube—A review on Synthesis, Properties and plethora of applications in the field of biomedical science. Sens. Int. 2020, 1, 100003. [Google Scholar] [CrossRef]
- Kumanek, B.; Janas, D. Thermal conductivity of carbon nanotube networks: A review. J. Mater. Sci. 2019, 54, 7397–7427. [Google Scholar] [CrossRef] [Green Version]
- Hayat, A.; Marty, J.L. Disposable Screen-Printed Electrochemical Sensors: Tools for Environmental Monitoring. Sensors 2014, 14, 10432–10453. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Anzar, N.; Hasan, M.R.; Akram, M.; Yadav, N.; Narang, J. Systematic and validated techniques for the detection of ovarian cancer emphasizing the electro-analytical approach. Process Biochem. 2020, 94, 126–135. [Google Scholar] [CrossRef]
- Alam, A.; Hasan, M.R.; Anzar, N.; Suleman, S.; Narang, J. Diagnostic approaches for the rapid detection of Zika virus—A review. Process Biochem. 2020, 101, 156–168. [Google Scholar] [CrossRef]
- Ambaye, A.D.; Kefeni, K.K.; Mishra, S.B.; Nxumalo, E.N.; Ntsendwana, B. Recent developments in nanotechnology-based printing electrode systems for electrochemical sensors. Talanta 2020, 225, 121951. [Google Scholar] [CrossRef]
- Sengupt, A. Electrodes. Metal Oxide Glass Nanocomposites; Elsevier: Amsterdam, The Netherlands, 2020; pp. 249–257. [Google Scholar]
- Singhal, C.; Dubey, A.; Mathur, A.; Pundir, C.; Narang, J. Paper based DNA biosensor for detection of chikungunya virus using gold shells coated magnetic nanocubes. Process Biochem. 2018, 74, 35–42. [Google Scholar] [CrossRef]
- Vasantham, S.; Alhans, R.; Singhal, C.; Nagabooshanam, S.; Nissar, S.; Basu, T.; Ray, S.C.; Wadhwa, S.; Narang, J.; Mathur, A. Paper based point of care immunosensor for the impedimetric detection of cardiac troponin I biomarker. Biomed. Microdevices 2019, 22, 6. [Google Scholar] [CrossRef]
- Mishra, A.; Pilloton, R.; Jain, S.; Roy, S.; Khanuja, M.; Mathur, A.; Narang, J. Paper-Based Electrodes Conjugated with Tungsten Disulfide Nanostructure and Aptamer for Impedimetric Detection of Listeria monocytogenes. Biosensors 2022, 12, 88. [Google Scholar] [CrossRef]
- Gupta, R.; Valappil, M.O.; Sakthivel, A.; Mathur, A.; Pundir, C.S.; Murugavel, K.; Narang, J.; Alwarappan, S. Tungsten disulfide Quantum Dots Based Disposable Paper Based Lab on GenoChip for Specific Meningitis DNA Detection. J. Electrochem. Soc. 2020, 167, 107501. [Google Scholar] [CrossRef]
- Glasscott, M.W.; Verber, M.D.; Hall, J.R.; Pendergast, A.D.; McKinney, C.J.; Dick, J.E. SweepStat: A build-it-yourself, two-electrode potentiostat for macroelectrode and ultramicroelectrode studies. J. Chem. Educ. 2020, 97, 265–270. [Google Scholar] [CrossRef] [Green Version]
- Teng, Y.; Chen, C.; Zhou, C.; Zhao, H.; Lan, M. Disposable amperometric biosensors based on xanthine oxidase immobilized in the Prussian blue modified screen-printed three-electrode system. Sci. China Ser. B Chem. 2010, 53, 2581–2586. [Google Scholar] [CrossRef]
- Mehto, N.K.; Sharma, P.; Kumar, S.; Khanuja, M.; Rawal, R.; Narang, J. Towards papertronics based electrode decorated with zinc oxide nanoparticles for the detection of the yellow fever virus consensus sequence. Process Biochem. 2022, 123, 36–43. [Google Scholar] [CrossRef]
- Narang, J.; Singhal, C.; Khanuja, M.; Mathur, A.; Jain, A.; Pundir, C.S. Hydrothermally synthesized zinc oxide nanorods incorporated on lab-on-paper device for electrochemical detection of recreational drug. Artif. Cells Nanomed. Biotechnol. 2018, 46, 1586–1593. [Google Scholar] [CrossRef]
- Liu, J.; Morris, M.D.; Macazo, F.; Schoukroun-Barnes, L.R.; White, R.J. The Current and Future Role of Aptamers in Electroanalysis. J. Electrochem. Soc. 2014, 161, H301–H313. [Google Scholar] [CrossRef]
- Chen, H.-L.; Hsiao, W.-H.; Lee, H.-C.; Wu, S.-C.; Cheng, J.-W. Selection and Characterization of DNA Aptamers Targeting All Four Serotypes of Dengue Viruses. PLoS ONE 2015, 10, e0131240. [Google Scholar] [CrossRef]
- Singh, S.; Hasan, M.R.; Sharma, P.; Narang, J. Graphene nanomaterials: The wondering material from synthesis to applications. Sens. Int. 2022, 3, 100190. [Google Scholar] [CrossRef]
- Bishoyi, A.; Alam, M.A.; Hasan, M.R.; Khanuja, M.; Pilloton, R.; Narang, J. Cyclic voltammetric- Paper-Based Genosensor for detection of the target DNA of zika virus. Micromachines 2022, 13, 2037. [Google Scholar] [CrossRef]
- Nassi, A.; Guillon, F.-X.; Amar, A.; Hainque, B.; Amriche, S.; Maugé, D.; Markova, E.; Tsé, C.; Bigey, P.; Lazerges, M.; et al. Electrochemical DNA-biosensors based on long-range electron transfer: Optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode. Electrochim. Acta 2016, 209, 269–277. [Google Scholar] [CrossRef]
- Anzar, N.; Suleman, S.; Kumar, R.; Rawal, R.; Pundir, C.S.; Pilloton, R.; Narang, J. Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver Nanoparticles. Micromachines 2022, 13, 1845. [Google Scholar] [CrossRef] [PubMed]
S.No | Involvements | Two-Electrode System (a) | Three-Electrode System (b) |
---|---|---|---|
1. | Total electrodes | Two electrodes | Three electrodes |
2. | Name of electrodes | -Working electrode (WE) -Counter electrode (CE) | -Working electrode (WE) -Counter electrode (CE) -Reference electrode (RE) |
3. | Ink/paste | Black carbon conductive ink | -Black carbon conductive -Silver paste casting for reference electrode |
4. | Substrate | Paper (A-4-size sheets) | Paper (A-4-size sheets) |
5. | Fabrication method | Screen-printing method | Screen-printing method |
6. | Incubation time | Overnight | Overnight |
7. | Electrochemicalmeasurement for dengue detection | Cyclic voltammetry technique | Cyclic voltammetry technique |
8. | Current range | Low | Very high |
9. | Expected price per electrode (INR) | 2 rupees (approx.) | 5 rupees (approx.) |
10. | Preparation time | 3 min | 5 min |
11. | Usability | Prepare, use, and throw | Prepare, use, and throw |
S.No | Different Stages | Current (Two-Electrode Setup) | Current (Three-Electrode Setup) |
---|---|---|---|
1. | Nanocomposites | 4.54 µA | 322.21 µA |
2. | Aptamer | 3.07 µA | 223.04 µA |
3. | Antigen | 2.48 µA | 161.44 µA |
4. | Bare | 0.85 µA | 55.53 µA |
S.No | Dengue Antigen Concentrations | Current (Two-Electrode Setup) | Current (Three-Electrode Setup) |
1. | 0.1 µg/mL | 2.48 µA | 161.44 µA |
2. | 1 µg/mL | 2.10 µA | 145.57 µA |
3. | 10 µg/mL | 1.86 µA | 139.20 µA |
4. | 100 µg/mL | 1.52 µA | 130.19 µA |
Biosensor | Electrode System | Electrodes involved | Current Range (Approx.) | References |
---|---|---|---|---|
Electrochemical biosensor (zika virus) | Two-electrode system | Counter and working electrodes | 2 µA | [20] |
Electrochemical biosensor (yellow fever) | Three-electrode system | Counter, working, and reference electrodes | 180 µA | [15] |
Electrochemical biosensor (dengue virus) | Three-electrode system | Counter, working, and reference electrodes | 322.21 µA | This work |
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Hasan, M.R.; Sharma, P.; Shaikh, S.; Singh, S.; Pilloton, R.; Narang, J. Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination. Biosensors 2023, 13, 1. https://doi.org/10.3390/bios13010001
Hasan MR, Sharma P, Shaikh S, Singh S, Pilloton R, Narang J. Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination. Biosensors. 2023; 13(1):1. https://doi.org/10.3390/bios13010001
Chicago/Turabian StyleHasan, Mohd. Rahil, Pradakshina Sharma, Shifa Shaikh, Saumitra Singh, Roberto Pilloton, and Jagriti Narang. 2023. "Electrochemical Aptasensor Developed Using Two-Electrode Setup and Three-Electrode Setup: Comprising Their Current Range in Context of Dengue Virus Determination" Biosensors 13, no. 1: 1. https://doi.org/10.3390/bios13010001