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Article

Colorant Pigments, Nutrients, Bioactive Components, and Antiradical Potential of Danta Leaves (Amaranthus lividus)

1
Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
2
Laboratory of Field Science, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
3
Department of Horticulture, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey
4
Food Engineering Department, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania
*
Authors to whom correspondence should be addressed.
Antioxidants 2022, 11(6), 1206; https://doi.org/10.3390/antiox11061206
Submission received: 16 May 2022 / Revised: 15 June 2022 / Accepted: 15 June 2022 / Published: 20 June 2022
(This article belongs to the Special Issue New Insights into Phytochemical Antioxidants in Food)

Abstract

:
In the Indian subcontinent, danta (stems) of underutilized amaranth are used as vegetables in different culinary dishes. At the edible stage of the danta, leaves are discarded as waste in the dustbin because they are overaged. For the first time, we assessed the colorant pigments, bioactive components, nutrients, and antiradical potential (AP) of the leaves of danta to valorize the by-product (leaf) for antioxidant, nutritional, and pharmacological uses. Leaves of danta were analyzed for proximate and element compositions, colorant pigments, bioactive constituents, AP (DPPH), and AP (ABTS+). Danta leaves had satisfactory moisture, protein, carbohydrates, and dietary fiber. The chosen danta leaves contained satisfactory magnesium, iron, calcium, potassium, manganese, copper, and zinc; adequate bioactive pigments, such as betacyanins, carotenoids, betalains, β-carotene, chlorophylls, and betaxanthins; and copious bioactive ascorbic acid, polyphenols, flavonoids, and AP. The correlation coefficient indicated that bioactive phytochemicals and colorant pigments of the selected danta leaves had good AP as assessed via ABTS+ and DPPH assays. The selected danta leaves had good ROS-scavenging potential that could indicate massive possibilities for promoting the health of the nutraceutical- and antioxidant-deficit public. The findings showed that danta leaves are a beautiful by-product for contributing as an alternate origin of antioxidants, nutrients, and bioactive compounds with pharmacological use.

1. Introduction

Amaranth is a promising crop with a wide divergence [1,2,3,4,5,6,7] Amaranth contains 20 times more calcium, 13 times more extra ascorbic acid, 7 times more iron, and 18 times more vitamin A precursor than lettuce [8]. This rapidly growing crop has C4 photosynthesis and versatile uses, namely as vegetables, ornamentals, and grains. It has broad adaptation and is dispersed in the United States of America, Africa, Europe, Asia, and Australia. Amaranth is a low-priced vegetable. Its stems and leaves are edible and have copious ascorbic acid; protein, with lysine and methionine amino acids essential for human nutrition [9,10,11,12,13,14,15,16,17]; carotenoids; digestible fiber; and minerals, including calcium, copper, magnesium, zinc, potassium, iron, and manganese [18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45]. Amaranthus has been used as folk medicine, particularly in anthelminthic [46,47,48], antimicrobial [49,50,51,52,53,54,55,56,57,58], anti-inflammatory [59,60,61], anticancer [62,63,64,65], hepatoprotective [66,67,68,69], antilipidemic [70,71,72,73,74,75], antimalarial [76], antidiabetic [77], antiviral [78], neuroprotective [79], antiulcer [80], and snake antidote contexts, among others. [81,82,83,84,85]. It also has abundant pigments, including carotenoids, betacyanins, betalains, betaxanthins, and chlorophylls [86,87,88,89,90,91,92,93,94,95,96,97] with high AP [98,99,100,101,102,103,104,105,106,107,108,109,110,111], phytochemicals including ascorbic acids, flavonoids, phenolic acids [112,113,114,115,116,117] with high AP [118,119,120,121,122,123,124,125,126,127,128,129,130]. These natural-origin compounds can quench reactive oxygen species (ROS) [131,132,133,134,135,136,137,138,139,140,141,142,143,144,145,146,147,148,149] and predominantly influence the industry of foods [96,97]. Pigments of amaranth are able to efficiently quench radicals [103,104,105,106,107,108,109]. Amaranth is widely acclimated to the environmental stresses of drought [150,151,152,153,154,155,156,157,158] and salinity [159,160,161,162,163].
The scarcity of calories and food insecurity has resulted in malnourishment in 79.5 crore people worldwide [164]. Approximately two crore people are affected by hidden hunger due to deficiency of minerals or vitamins [165]. Staple foods are the principal source of energy but have deficits of iron, α-carotene, zinc, β-carotene, iodine, other carotenoids, ascorbic acid, and vitamin E [166]. Consuming staple foods regularly results in hidden hunger [165]. However, consuming staple foods alongside vegetables and fruits ensures a healthy diet with a balanced vitamin and mineral source. Phytochemical compounds, including ascorbic acid, pigments, and phenolics, have significant contributions to health promotion [167,168,169].
Currently, antioxidants from natural sources, especially vegetables, have attracted the attention of consumers and researchers. Amaranth bioactives comprise β-carotene, flavonoids, vitamin C, and phenolics, which have radical quenching potential [96,97]. These bioactive components defend against numerous diseases, such as cataracts, cardiovascular diseases, atherosclerosis, emphysema, arthritis, cancer, retinopathy, and degenerative diseases of the neuron [169,170,171,172]. Natural products capable of antioxidant properties have gained substantial interest.
Danta (Amaranthus lividus) is a wild and edible vegetable. It is found as wild vegetables throughout the world, including in its native habitat. However, in the Indian subcontinents, it has grown as a wild and cultivated form. The diversified germplasm of amaranth indicates its enormous variability and plasticity in phenotype [173,174,175,176,177,178,179,180,181,182,183,184,185,186,187,188,189,190,191,192,193,194,195], which has multipurpose uses. The attractive flavor, color, and taste make it a standard vegetable in different culinary dishes in the Indian subcontinent. It can be grown throughout the year on the Indian subcontinent in vegetable gap periods between winter and hot summer [9,10]. The succulent, juicy, and sizeable barreled stem becomes edible as a vegetable 50–60 days after sowing and can be consumed until seed maturity. This stem is popularly known as danta. Generally, flowering starts at around 2–3 months of age, though cultivars sensitive to the photoperiod flower at around 9 months to 1 year. The big and flashy stem is consumed year-round as a very famous vegetable in India and Bangladesh. The leaves of danta are not consumed, because at the time of harvesting of danta (the edible stage of the stem), the leaves are aged. They are thus left in the dustbin as waste materials. However, the by-product leaves have an attractive ROS scavenging potential. They could be used as an alternate origin of nutrients and bioactive compounds for the benefits of health promotion and nourishing the people’s scarcity in antioxidants and nutraceuticals. The literature has shown that amaranth leaves are a plentiful origin of pigments, nutrients, antioxidants, minerals, and phytochemicals compared with the stem [96,108]. For this reason, we herein investigated the possibility of exploiting danta leaves as a source of nutraceuticals and natural pigments because of their sufficient betacyanins, betaxanthins, betalains, and phytochemicals of interest in the food industry [93,94,95]. Therefore, for the first time, we investigated the nutraceuticals, phytochemicals, pigments, and antioxidant potential of the selected danta leaves to valorize the leaves as antioxidants, bioactive compounds, and nutrient sources.

2. Materials and Methods

2.1. Materials of the Study

The Department of Genetics and Plant Breeding of Bangabandhu Sheikh Mujibur Rahman Agricultural University preserved many amaranth germplasm accessions in the gene bank. In our previous studies, we evaluated many amaranth accessions for their agronomic and antioxidant potentials [9,10,11,12,95]. Finally, four advanced accessions (SA5, SA8, SA9, and SA17) were selected based on the yields and antioxidant potentials evaluated in our previous studies.

2.2. Layout and Design

The investigation was carried out following a randomized design with three blocks (3 replicates) at BSMRAU. Each experimental unit comprised a 1 m2 plot using row and plant spacing of 25 cm and 10 cm, respectively.

2.3. Intercultural Practices

Appropriate cultural practices and recommended doses of fertilization with inorganic fertilizer and organic compost were maintained. The spacing of plants was continued following appropriate thinning. Weeds were eradicated with hoeing at regular intervals. Regular irrigation was provided to uphold the adequate growth of plants. At 60 days of age, the leaf samples were collected from plants. The leaves were washed thoroughly in tap water. Water on the surface of washed leaves was removed by spreading the leaves on a clean board in a well-ventilated room at room temperature until the water was removed from the leaves through evaporation. Then, leaves were used for further phytochemical extraction from the fresh sample.

2.4. Reagents and Solvents

Reagents: H2SO4, cesium chloride, dithiothreitol (DTT), HClO4, ascorbic acid, HNO3, ABTS+, Trolox, Folin–Ciocâlteu reagent, rutin, DPPH, 2,2-dipyridyl, AlCl36H2O, gallic acid, CH3CO2K, potassium persulfate, and Na₂CO₃. Solvent: Methanol, hexane, and acetone. All solvents and reagents used in this study were high purity laboratory products obtained from Kanto Chemical Co. Inc. (Tokyo, Japan) and Merck (Darmstadt, Germany).

2.5. Estimation of Proximate Composition

Ash, fat, moisture, fiber, protein, and energy were determined using the AOAC method [196]. Nitrogen was calculated following the micro-Kjeldahl method (AOAC method 976.05). The protein was determined by multiplying the N value by 6.25. The total fat, ash, moisture, and protein (%) were deducted from one hundred (100) to estimate the carbohydrates (g 100 g−1 fresh weight (FW)).

2.6. Estimation of Mineral Composition

The leaves were dried for 24 h in an oven maintaining 70 °C temperature and ground in a mill. Magnesium, potassium, calcium, manganese, copper, zinc, and iron were determined from leaf powder. The samples (0.5 g) were digested with 400 mL HNO3 (65%), 40 mL HClO4 (70%), and 10 mL H2SO4 (96%) [197]. An atomic absorption spectrophotometry (AAS) device (Hitachi, Tokyo, Japan) was used to read the absorbance [61] at 285.2 (Mg), 213.9 (Zn), 766.5 (K), 248.3 (Fe), 279.5 (Mn), 422.7 (Ca), and 324.8 (Cu) nm wavelengths.

2.7. Carotenoids and Chlorophylls Determination

Chlorophylls (ab, b, and a) and carotenoids were determined by extracting the samples in C3H6O (80%) [198]. A Hitachi spectrophotometer (Tokyo, Japan) was used to estimate the absorbance at 646, 663, and 470 nm for carotenoids and chlorophyll b and a, respectively. Carotenoids were calculated as milligrams per 100 g, and chlorophyll was calculated as micrograms per gram of FW.

2.8. Determination of Betacyanins and Betaxanthins

We extracted the leaves in MeOH (80%) comprising ascorbic acid (50 mM) [199,200,201]. The betacyanins and betaxanthins were estimated using a spectrophotometer at 540 and 475 nm wavelengths. The results were calculated as nanograms of betanin and indicaxanthin equivalent per gram of FW for betacyanins and betaxanthins.

2.9. Estimation of β-Carotene

Exactly 500 mg of fresh leaves was thoroughly ground with 10 mL C3H6O (80%) in a mortar and pestle and centrifuged for 3–4 min at 10,000 × g to estimate β-carotene [150]. The supernatant was transferred to a volumetric flask and marked up to 20 mL. A Hitachi spectrophotometer (Tokyo, Japan) was used to take the absorbance at 510 and 480 nm, respectively. β-carotene was determined as mg of β-carotene/100 g FW.

2.10. Estimation of Ascorbic Acid

A spectrophotometer was set to determine DHA and AsA from fresh samples of the leaf. Dithiothreitol (DTT) was utilized to preincubate the sample. Dithiothreitol (DTT) reduced dehydroascorbic acid to ascorbic acid. As a result of the ascorbic acid reduction, a ferrous ion was formed from the ferric ion. Reduced ferrous ions react with 2,2-dipyridyl to form complexes [150]. To estimate ascorbic acid, the absorbance of Fe2+ complexes with 2,2-dipyridyl was read at 525 nm using a spectrophotometric (Hitachi, Tokyo, Japan). The ascorbic acid was calculated in mg/100 g FW.

2.11. Extraction of Samples and Estimation of TP, AP, and TF

The fresh and dried ground leaves (60 d) were used to produce extract in a mortar and pestle for estimation of AP, total flavonoids (TF), and total polyphenols (TP). Leaves (0.25 g) were added in 90% MeOH (10 mL) in a tightly capped bottle and placed at 60 °C in a water bath (Tokyo, Japan) for 1 h. We filtered the extract and kept it for the estimation of AP, TF, and TP. Folin–Ciocâlteu reagent and the aluminum chloride colorimetric method were used to estimate polyphenols [202] and flavonoids [203,204], respectively. A spectrophotometer (Hitachi, Tokyo, Japan) was used to take the absorbance at 760 and 415 nm for TP and TF, respectively. A standard gallic acid curve (Y = 0.009X + 0.019) and rutin curve (Y = 0.013X) were made, and TP and TF were estimated as μg GAE g−1 of FW and μg RE g−1 DW, respectively. The diphenyl-picrylhydrazyl (DPPH) radical degradation method was used to estimate AP (DPPH) [150]. The method of Khanam et al. [205] was used to perform the ABTS+ assay. DPPH and ABTS+ inhibition percentages corresponding to the control were followed to estimate AP using the equation:
AP (%) = (AB − ALS/AB) × 100
where AB is the optical density of the blank [as a substitute of leaf extract 10 µL and 150 μL MeOH for AP (DPPH and ABTS), respectively] and ALS is the optical density of the leaf samples. The data are expressed as Trolox equivalent (TE) μg g−1 DW.

2.12. Statistical Analysis

The averaged data from each replication constituted the replication mean. The Statistix 8 software was used to analyze the data for analysis of variance (ANOVA) [206,207,208]. The means data were compared using the Duncan multiple range test at a probability of 1%. The data are presented as the mean ± SD.

3. Results and Discussion

Analysis of variance indicated noteworthy variation among parameters for all characters. Widespread differences were also revealed in the biochemical traits of amaranth [197,198,199].

3.1. Composition of Proximate

Figure 1 shows the composition of moisture, fat, carbohydrates, protein, ash, fiber (g 100 g−1 FW), and energy (kcal 100 g−1 FW) of danta leaves. The moisture of danta leaves differed from 81.47 in SA8 to 82.75 in SA17. As lower moisture content confers higher leaf dry matter; hence, some accessions had considerable dry biomass (18–19% dry mass). The moisture of leaves is straightly interrelated to maturity. The results from these danta leaves were in agreement with those from A. tricolor [10] and sweet potato leaves [209].
As for vegetables, danta leaves had a high protein that prominently differed among accessions (3.56 to 6.21). As leafy vegetables, greater protein content was observed in SA8, SA5, and SA9. Vegetarians and poor people in underdeveloped countries mostly trust danta leaves as a source of protein. Danta leaves displayed a much greater protein content than A. tricolor (1.26%) [10]. Danta leaves displayed low fat content owing to being a vegetable and can be consumed as a fat-free food. Danta genotypes varied significantly for leaf fat content (0.15–0.25), which results were corroborated by those for A. tricolor [10] and sweet potato [209]. The authors of [10,209] noted that fat upholds the temperature of the body, covers organs, and influences cell function. Fats are sufficient sources of Ω-6 and Ω-3 fatty acids and give a noteworthy contribution to the transport, digestion, and absorption of the lipid-soluble vitamins K, E, D, and A.
Danta leaves had good carbohydrate content, with ample variation regarding accessions (6.49 to 9.00). SA8 displayed the highest carbohydrate content (9.00), and high carbohydrate content was recorded in SA5 and SA9, while SA17 exhibited the minimum carbohydrates (6.49). The leaves of danta genotypes also had diverse energy content (49.78 to 55.35). Danta leaves of SA8 had the maximum energy (55.35), and high energy was recorded in SA5 and SA9. On the other hand, danta leaves of SA17 had the minimum energy (49.78). SA8 had the highest ash content (5.66); conversely, SA17 had the minimum ash content (4.54).
The dietary fiber also differed among the danta leaves (6.74 to 9.21). Danta leaves of SA17 had the maximum dietary fiber (9.21), followed by SA9. Conversely, SA8 had the minimum dietary fiber (6.74). Dietary fiber remarkably augments digestibility, constipation, and palatability [12]. Danta leaves were rich in protein, dietary fiber, carbohydrates, and moisture. Our earlier study was in agreement with the current findings [10]. The carbohydrate content of the advanced line of danta leaves of SA17 and the protein content of the danta leaves of SA5, SA8, and SA9 were superior to the protein and carbohydrate contents of red amaranth [210], green amaranth [211], weedy amaranth [212], and danta [213]. The dry matter obtained from the advanced line of danta leaves was superior to the dry matter of red amaranth [210], green amaranth [211], A. spinosus [212], and danta [213], whereas it was surpassed by the dry matter of A. viridis weedy amaranth [212]. The digestible fiber content of the danta leaves of SA17 was higher than that of red, green, and danta [210,211,212,213] but lower than that of weedy amaranth (A. spinosus) and comparable to that of weedy amaranth (A. viridis) [212].

3.2. Mineral Elements

Figure 2 shows the mineral elements, both macro- (mg g−1 FW) and microelements (µg g−1 FW), of the studied danta leaves. The danta leaves had good content of potassium. Danta leaves of SA5 had the maximum potassium (4.22), which was statistically parallel to that of SA9. The minimum potassium was recorded in SA17 (3.45). The calcium greatly varied among accessions (1.72 to 3.12). Danta leaves of SA9 showed the maximum calcium (3.12 mg g−1). In contrast, SA17 displayed the minimum calcium (1.72). The danta leaves had good magnesium content, and variations were not prominent regarding accessions (2.73 to 3.45). SA5 had the highest magnesium (3.45). In contrast, SA8 had the minimum magnesium (2.73). We documented sufficient K (4.22), Mg (3.45), and Ca (3.12) in the danta leaves. Several species of amaranth had ample Mg, Ca, and K [202]. These findings also showed that the calcium, potassium, and magnesium in amaranth were much more noticeable than those in spinach, nightshade, black kale, and spider flower. The potassium content of danta leaves was comparable to that of green amaranth [211] but less than that of weedy amaranth [212]. The Ca detected in danta leaves of SA9 was superior to that in weedy amaranth [212], green morph amaranth [211], and A. blitum [214]. The magnesium noticed in the danta leaves was superior that of green amaranth [211] and comparable to that of weedy amaranth [212].
Danta leaves displayed wide variations among accessions for Fe content (10.96 to 17.28). SA5 had the maximum Fe (17.28), and SA17, the minimum (10.96). In our study, great differences were observed in the manganese of danta leaves (3.07 and 5.72). SA17 exhibited the highest manganese (5.72), and SA9, the lowest (3.07). The copper had noteworthy differences among the selected danta leaves (1.29 to 2.62). SA9 had the highest Cu (2.62), and SA8 had the lowest Cu (1.29). Zinc content diverged among accessions (6.23 in SA8 to 8.96 in SA5). The iron and zinc content of the selected danta leaves were superior to those of cassava leaves [215] and beach pea [216]. We noted sufficient Fe (17.28), Mn (5.72), and Zn (8.96) and noteworthy Cu (2.62) in the selected danta leaves. In the literature on several species of amaranth, sufficient Mn, Fe, Zn, and Cu were noted [202]. The authors of [202] also showed that leaves of amaranth species had more noticeable Mn, Zn, Fe, and Cu than kale, spinach, spider flower, and black nightshade. In the current investigation, the iron content of danta leaves of SA5 were much superior to that of A. spinosus and green amaranth [211,212], even though the iron content of this line was less than that of A. viridis [212]. The manganese content of danta leaves was less than that of green and weedy amaranth [211,212]. The copper content of danta leaves was much superior to that of green amaranth [211] but less than that of weedy amaranth [212]. The zinc content of danta leaves was less than that of weedy and green amaranth [211,212].

3.3. Bioactive Pigments

Figure 3 shows the bioactive pigments, such as carotenoids (mg 100 g−1 FW), chlorophylls (μg g−1 FW), and betalains (ng g−1 FW), of danta leaves. Danta leaves exhibited great variation in chlorophyll a (cha) content (131.56 to 474.51). SA8 had the highest cha (474.51), followed by SA9. In contrast, SA5 had the lowest cha (131.56). Danta leaves exhibited great variation in chlorophyll b (chb) content (62.42 to 278.11). SA8 displayed the highest chb (278.11), followed by SA9. In contrast, SA5 had the lowest chb (62.42). Significant and considerable differences in chlorophyll ab (chab) were observed in danta leaves (194.99 to 753.73). SA8 displayed the highest chab (753.73), followed by SA9. In contrast, SA5 had the lowest chab (194.99). Notable contents of cha (474.51), chab (753.73), and chb (278.11), which was superior to those of green and red amaranth [217], were observed in the selected danta leaves. The observed cha, chb, and chab levels were much superior to those of red, green, weedy, and danta leaves [210,211,212,213] of our earlier studies.
Danta leaves had good content of betacyanins, with significant variability regarding accessions (185.52 to 338.51). SA9 had the highest betacyanins (338.51). On the other hand, SA5 had the minimum betacyanins (185.52). Danta leaves had good betaxanthins, with noteworthy variability regarding accessions (181.90 to 354.31). SA9 had the highest betaxanthins (354.31). Conversely, SA5 had the minimum betaxanthins (181.90). Danta leaves had good betalain content, with prominent variability regarding accessions (367.35 to 692.74). The betalains were the highest in SA9 (692.74) and the lowest in SA5 (367.35). The carotenoids exhibited significant variation among the accessions (49.39 to 125.17). The highest carotenoids were noted in SA17 (125.17), and the lowest were noted in SA9 (49.39). Notable levels of cha (474.51), carotenoids (125.17), betacyanins (338.51), betalains (692.74), betaxanthins (354.31), and chb (278.11) were recorded in the selected danta leaves, which were comparable to those in green and red amaranth [217]. The betacyanins of danta leaves were much more noticeable than those of green weedy and danta leaves [211,212,213] and comparable to those of red morph amaranth [210]. The betaxanthins and betalains of danta leaves were much more noticeable than those of green morph amaranth [211]. The betaxanthins and betalains in SA9 were much more noticeable than those in red, weedy, and danta leaves [210,211,212,213]. The carotenoid content of SA17 was greater than that of red, green, weedy, and danta leaves [210,211,212,213].

3.4. Bioactive Components and AP

The total polyphenols (TP, µg g−1 FW), β-carotene (mg 100 g−1 FW), total flavonoids (TF, μg g−1 DW), ascorbic acid (mg 100 g−1 FW), and AP (μg g−1 DW) of the selected danta leaves are presented in Figure 4. Considerable variation was documented in the β-carotene of the danta leaves (35.54 in SA9 to 57.83 in SA5). The highest β-carotene was observed in SA17 (56.42). The danta leaves also had considerable variation in ascorbic acid (61.63 to 128.68). SA8 displayed the highest ascorbic acid (128.68), and SA9, the lowest (61.63). Noticeable and significant variations were noted in the TP of the selected danta leaves (15.66 to 25.35). SA8 had the highest TP (25.35), followed by SA17; SA5 had the lowest TP (15.66). The selected danta leaves had high TF, with noteworthy differences among genotypes (142.35 to 153.48). SA17 had the highest TF (153.48), which had a statistical similarity to SA5. SA9 had a high TF (151.36), whereas SA8 had the lowest TF (142.35). High DPPH and ABTS+ AP were recorded in the danta leaves, with low variability regarding accessions. SA17 had the highest DPPH and ABTS+ AP (27.96, 52.64), followed by SA8 (25.06, 46.14) and SA5 (24.92, 45.57).
The minimum ABTS+ and DPPH AP were recorded in SA9 (23.26, 42.95). The similar tendencies of AP under the ABTS+ and DPPH methods authenticated the antioxidant capacities measured via the two methods. In the current investigation, the selected danta leaves displayed notable ascorbic acid and β-carotene contents (128.68 and 57.83), which were greater than those of red amaranth [10]. The TP (25.35), AP in DPPH (27.96), TF (153.48), and AP in ABTS+ (52.64) obtained were substantiated with green and red amaranth [199]. The β-carotene of the danta leaves was comparable to that of weedy amaranth [212] but lower than that previously measured in danta leaves [213] and that in red morph amaranth [210] in our earlier studies. The ascorbic acid obtained from the SA8 danta leaves was superior to that of red, weedy, stem, and green amaranth [210,211,212,213]. The TP of danta leaves was superior to that of green morph amaranth [210] and comparable to that of weedy amaranth (A. spinosus) [212]. The TF and AP (ABTS+ and DPPH) of the danta leaves were superior to those of green, red, and danta leaves [210,211,214] and comparable to those of weedy amaranth [212]. The selected danta leaves had high levels of antioxidants, phenolics, and flavonoids, along with substantial nutrients, photopigments, and vitamins. These accessions can be used as preferable high-yielding cultivars containing sufficient antioxidants and appropriate for extracting colorful juice. The investigation exposed that danta leaves were a great source of nutrients and phytochemicals with antioxidant activities and presented enormous potential as food for people deficient in minerals, vitamins, and antioxidants.

3.5. Association Studies

The relationships of the bioactive pigments, TF, β-carotene, AP (ABTS+), TP, AP (DPPH), and ascorbic acid of danta leaves are shown in Table 1. The associations of the bioactive colorant pigments, ascorbic acid, AP (DPPH), TP, β-carotene, TF, and AP (ABTS+) of danta leaves had stimulating outcomes. All bioactive colorant pigments were significantly and positively associated with TP, AP (DPPH), TF, and AP (ABTS+). This indicated that increases in TP, AP (ABTS+), TF, and AP (DPPH) were unswervingly associated with the augmentation of betaxanthins, carotenoids, chlorophylls, betalains, and betacyanins or vice versa. It also confirmed that all bioactive colorant pigments had strong AP.
Likewise, ascorbic acid had significantly positive associations with TP, AP (DPPH), TF, and AP (ABTS+), although it displayed negative and insignificant relationships with all bioactive colorant pigments. Sarker and Oba [150,161] detected a parallel tendency. Positive and significant correlations were observed with ascorbic acid, TP, AP (DPPH), TF, β-carotene, and AP (ABTS+), which was corroborative to past results on salt-induced amaranth [218,219,220,221,222]. The positive and noteworthy correlations of ascorbic acid, AP (DPPH), TP, β-carotene, TF, and AP (ABTS+) suggested that β-carotene, TF, ascorbic acid, and TP had strong AP. The authentication of the AP of the danta leaves by two methods of AP was established, with positive and noteworthy correlations between AP (DPPH) and AP (ABTS+). Bioactive pigments and phytochemicals such as β-carotene, TF, TP, and ascorbic acid had strong AP, as confirmed by significant relationships with AP (DPPH) and AP (ABTS+). All bioactive pigments, TP, ascorbic acid, TF, and β-carotene displayed vital contributions to the AP of danta leaves, because the compounds had strong AP.

4. Conclusions

The leaves of danta leaves had ample sources of Mg, K, carbohydrates, Ca, Fe, dietary fiber, Cu, protein, Zn, and Mn. They were an admirable origin of bioactive pigments such as betacyanins, β-carotene, betalains, ascorbic acid, betaxanthins, carotenoids, TP, chlorophylls, TF, and antioxidants. The correlation coefficient revealed that the bioactive pigments and phytochemicals of danta leaves had good AP (ABTS+) and AP (DPPH). Danta leaves are an underutilized but promising vegetable. Danta leaves had enormous bioactive phytochemicals and antioxidants, which could be cultivated in preferable cultivars. The leaves could be utilized as boiled food, fresh salads, leafy vegetables for daily diet, and other culinary dishes. Considering the status of their nutrients, they could be equivalent to spinach. They could also be grown year-round, including in summer, a gap in vegetable growth. The leaves could be used for the extraction of colorful juice as a possible origin of nutritional value, bioactive pigments, phenolics, ascorbic acid, flavonoids, β-carotene, and antioxidants in a regular diet to accomplish antioxidant and nutritional sufficiency. The selected danta leaves contained good ROS-scavenging potential that offered enormous prospects for health promotion in the antioxidant- and nutraceutical-deficit community. We concluded that danta leaves are an attractive by-product to contribute as an alternative source of nutrients, bioactive compounds, and antioxidants.

Author Contributions

Conceptualization, U.S.; writing—original draft preparation, U.S., S.O., M.A.I., M.N.H., S.E., C.C.M. and R.A.M.; data curation, U.S., validation, U.S., M.A.I., M.N.H., S.O., S.E., C.C.M. and R.A.M.; visualization, U.S.; writing—review and editing, U.S., S.O., S.E., C.C.M. and R.A.M.; investigation, U.S.; methodology, U.S. and S.O.; supervision, U.S.; resources, U.S.; software, U.S. and S.O; formal analysis, U.S. and S.O. All authors have read and agreed to the published version of the manuscript.

Funding

The current work was funded by the Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh and the National Research Development Projects to finance excellence (PFE)-14/2022-2024 granted by the Romanian Ministry of Research and Innovation.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data is contained within the article.

Acknowledgments

For supporting the research work, the authors acknowledge the Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh. The authors would like to extend their sincere appreciation to the National Research Development Projects to finance excellence (PFE)-14/2022-2024 granted by the Romanian Ministry of Research and Innovation.

Conflicts of Interest

All the authors have no conflict of interest.

References

  1. Rastogi, A.; Shukla, S. Amaranth: A New Millennium Crop of Nutraceutical Values. Crit. Rev. Food Sci. Nutr. 2013, 53, 109–125. [Google Scholar] [CrossRef] [PubMed]
  2. Das, S. Amaranths: The Crop of Great Prospect. In Amaranthus: A Promising Crop of Future; Springer: Singapore, 2016; pp. 13–48. [Google Scholar]
  3. Sreelathakumary, I.; Peter, K.V. Amaranth: Amaranthus spp. In Genetic Improvement of Vegetable Crops; Elsevier: Amsterdam, The Netherlands, 1993; pp. 315–323. [Google Scholar]
  4. Sauer, J.D. The Grain Amaranths and Their Relatives: A Revised Taxonomic and Geographic Survey. Ann. Missouri Bot. Gard. 1967, 54, 103. [Google Scholar] [CrossRef]
  5. Anu, R.; Mishra, B.K.; Mrinalini, S.; Ameena, S.; Rawli, P.; Nidhi, V.; Sudhir, S. Identification of Heterotic Crosses Based on Combining Ability in Vegetable Amaranthus (Amaranthus tricolor L.). Asian J. Agric. Res. 2015, 9, 84–94. [Google Scholar]
  6. Nguyen, D.C.; Tran, D.S.; Tran, T.T.H.; Ohsawa, R.; Yoshioka, Y. Genetic Diversity of Leafy Amaranth (Amaranthus tricolor L.) Resources in Vietnam. Breed. Sci. 2019, 69, 640–650. [Google Scholar] [CrossRef] [Green Version]
  7. Shukla, S.; Bhargava, A.; Chatterjee, A.; Srivastava, A.; Singh, S.P. Genotypic Variability in Vegetable Amaranth (Amaranthus tricolor L for Foliage Yield and Its Contributing Traits over Successive Cuttings and Years. Euphytica 2006, 151, 103–110. [Google Scholar] [CrossRef]
  8. Guillet, D. Grain Amaranthus, History, and Nutrition. Kokopelli Seed Foundation. 2004. Available online: http://www.kokopelli-seed-foundation.com/amaranths.htm (accessed on 12 October 2021).
  9. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Genotypic Variability for Nutrient, Antioxidant, Yield and Yield Contributing Traits in Vegetable Amaranth. J. Food Agric. Environ. 2014, 12, 168–174. Available online: https://www.wflpublisher.com/Abstract/5378 (accessed on 15 June 2022).
  10. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Variability, heritability and genetic association in vegetable amaranth. Span. J. Agril. Res. 2015, 13, 0702. [Google Scholar] [CrossRef] [Green Version]
  11. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Variability in Composition of Vitamins and Mineral Antioxidants in Vegetable Amaranth. Genetika 2015, 47, 85–96. [Google Scholar] [CrossRef]
  12. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Genetic Variation and Interrelationships among Antioxidant, Quality, and Agronomic Traits in Vegetable Amaranth. Turk. J. Agric. For. 2016, 40, 526–535. [Google Scholar] [CrossRef]
  13. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Genotypic Diversity in Vegetable Amaranth for Antioxidant, Nutrient and Agronomic Traits. Indian J. Genet. Plant Breed. 2017, 77, 173–176. [Google Scholar] [CrossRef]
  14. Andini, R.; Yoshida, S.; Ohsawa, R. Variation in Protein Content and Amino Acids in the Leaves of Grain, Vegetable and Weedy Types of Amaranths. Agronomy 2013, 3, 391–403. [Google Scholar] [CrossRef] [Green Version]
  15. Manólio Soares, R.A.; Mendonça, S.; de Castro, L.I.A.; Menezes, A.C.C.C.C.; Gomes Arêas, J.A. Major Peptides from Amaranth (Amaranthus cruentus) Protein Inhibit HMG-CoA Reductase Activity. Int. J. Mol. Sci. 2015, 16, 4150. [Google Scholar] [CrossRef] [Green Version]
  16. Písaríková, B.; Krácmar, S.; Herzig, I. Amino acid Contents and Biological Value of Protein Amaranth. Czech J. Anim. Sci. 2005, 50, 169–174. [Google Scholar] [CrossRef] [Green Version]
  17. López, D.N.; Galante, M.; Raimundo, G.; Spelzini, D.; Boeris, V. Functional Properties of Amaranth, Quinoa and Chia Proteins and the Biological Activities of Their Hydrolyzates. Food Res. Int. 2019, 116, 419–429. [Google Scholar] [CrossRef] [Green Version]
  18. Shukla, S.; Bhargava, A.; Chatterjee, A.; Srivastava, J.; Singh, N.; Singh, S.P. Mineral Profile and Variability in Vegetable Amaranth (Amaranthus tricolor). Plant Foods Hum. Nutr. 2006, 61, 23–28. [Google Scholar] [CrossRef]
  19. Chakrabarty, T.; Sarker, U.; Hasan, M.; Rahman, M.M. Variability in Mineral Compositions, Yield and Yield Contributing Traits of stem amaranth (Amaranthus lividus). Genetika 2018, 50, 995–1010. [Google Scholar] [CrossRef] [Green Version]
  20. Alvarez-Jubete, L.; Arendt, E.K.; Gallagher, E. Nutritive Value of Pseudocereals and Their Increasing Use as Functional Gluten-Free Ingredients. Trends Food Sci. Technol. 2010, 21, 106–113. [Google Scholar] [CrossRef]
  21. Achigan-Dako, E.G.; Sogbohossou, O.E.D.; Maundu, P. Current Knowledge on Amaranthus spp.: Research Avenues for Improved Nutritional Value and Yield in Leafy Amaranths in Sub-Saharan Africa. Euphytica 2014, 197, 303–317. [Google Scholar] [CrossRef]
  22. Akin-Idowu, P.E.; Odunola, O.A.; Gbadegesin, M.A.; Ademoyegun, O.T.; Aduloju, A.O.; Olagunju, Y.O. Nutritional Evaluation of Five Species of Grain Amaranth—An Underutilized Crop. Int. J. Sci. 2017, 3, 18–27. [Google Scholar] [CrossRef] [Green Version]
  23. Alegbejo, J. Nutritional Value and Utilization of Amaranthus (Amaranthus spp.)—A Review. Bayero J. Pure Appl. Sci. 2014, 6, 136. [Google Scholar] [CrossRef] [Green Version]
  24. Shukla, S.; Bhargava, A.; Chatterjee, A.; Pandey, A.C.; Mishra, B.K. Diversity in Phenotypic and Nutritional Traits in Vegetable Amaranth (Amaranthus tricolor), A Nutritionally Underutilised Crop. J. Sci. Food Agric. 2010, 90, 139–144. [Google Scholar] [CrossRef] [PubMed]
  25. Soriano-García, M.; Ilnamiqui Arias-Olguín, I.; Carrillo Montes, J.P.; Genaro Rosas Ramírez, D.; Mendoza Figueroa, J.S.; Flores-Valverde, E.; Rita Valladares-Rodríguez, M. Nutritional Functional Value and Therapeutic Utilization of Amaranth. J. Anal. Pharm. Res. 2018, 7, 596–600. [Google Scholar] [CrossRef] [Green Version]
  26. Akubugwo, I.E.; Obasi, N.A.; Chinyere, G.C.; Ugbogu, A.E. Nutritional and Chemical Value of Amaranthus hybridus L. Leaves from Afikpo, Nigeria. Afr. J. Biotechnol. 2007, 6, 2833–2839. [Google Scholar] [CrossRef] [Green Version]
  27. Ezenwa, M.I.; Ogbadoyi, E.O. Effect of Heading on Some Micronutrients, Anti-Nutrients and Toxic Substances in Amaranthus cruentus Grown in Minna, Niger State, Nigeria. J. Food Nutr. Res. 2011, 1, 147–154. [Google Scholar]
  28. Lobo, M.; Samman, N.; Castanheira, I. Characterisation of Nutrient Profile of Quinoa (Chenopodium quinoa), Amaranth (Amaranthus caudatus), and Purple Corn (Zea mays L.) Consumed in the North of Argentina: Proximates, Minerals and Trace Elements. Food Chem. 2014, 148, 420–426. [Google Scholar]
  29. Nyonje, W.A.; Schafleitner, R.; Abukutsa-Onyango, M.; Yang, R.-Y.; Makokha, A.; Owino, W. Precision phenotyping and association between morphological traits and nutritional content in Vegetable Amaranth (Amaranthus spp.). J. Agric. Food Res. 2021, 5, 100165. [Google Scholar] [CrossRef]
  30. Schafleitner, R.; Lin, Y.P.; Dinssa, F.; N’Danikou, S.; Finkers, R.; Minja, R.; Abukutsa-Onyango, M.; Nyonje, W.; Lin, C.Y.; Wu, T.H.; et al. The world vegetable center Amaranthus germplasm collection: Core collection development and evaluation of agronomic and nutritional traits. Crop Sci. 2022, 62, 1173–1187. [Google Scholar] [CrossRef]
  31. Srivastava, R. Nutritional Quality of Some Cultivated and Wild Species of Amaranthus L. Int. J. Pharm. Sci. Res. 2011, 2, 3152. [Google Scholar]
  32. Wesche-Ebeling, P.; Maiti, R.; García-Díaz, G.; González, D.I.; Sosa-Alvarado, F. Contributions to the Botany and Nutritional Value of Some Wild Amaranthus species (Amaranthaceae) of Nuevo Leon, Mexico. Econ. Bot. 1995, 49, 423–430. [Google Scholar] [CrossRef]
  33. Mekonnen, G.; Woldesenbet, M.; Teshale, T.; Biru, T. Amaranthus caudatus Production and Nutrition Contents for Food Security and Healthy Living in Menit Shasha, Menit Goldya and Maji Districts of Bench Maji Zone, South Western Ethiopia. Nutr. Food Sci. Int. J. 2018, 7, 10–19080. [Google Scholar]
  34. Mlakar, S.G.; Turinek, M.; Jakop, M.; Bavec, M.; Bavec, F. Nutrition Value and Use of Grain Amaranth: Potential Future Application in Bread Making. Agriculturae 2009, 6, 43–53. [Google Scholar]
  35. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Suitability of Amaranthus species for Alleviating Human Dietary Deficiencies. S. Afr. J. Bot. 2018, 115, 65–73. [Google Scholar] [CrossRef]
  36. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Nutrients and Antinutrient Constituents of Amaranthus caudatus L. Cultivated on Different Soils. Saudi J. Biol. Sci. 2020, 27, 3570–3580. [Google Scholar] [CrossRef]
  37. Sokolova, D.; Shelenga, T.; Zvereva, O.; Solovieva, A. Comparative Characteristics of the Amino Acid Composition in Amaranth Accessions from the VIR Collection. Turk. J. Agric. For. 2021, 45, 6. [Google Scholar] [CrossRef]
  38. Sena, L.P.; Vanderjagt, D.J.; Rivera, C.; Tsin, A.T.; Muhamadu, I.; Mahamadou, O.; Glew, R.H. Analysis of Nutritional Components of Eight Famine Foods of the Republic of Niger. Plant Foods Hum. Nutr. 1998, 52, 17–30. [Google Scholar] [CrossRef]
  39. Mohil, P.; Jain, U. Quantitative Analysis of Minerals in Certain Species of Amaranthus. Indian J. Plant Sci. 2012, 1, 213–216. [Google Scholar]
  40. Mohammed, M.I.; Sharif, N. Mineral Consumption of Some Leafy Vegetables Consumed in Kano, Nigeria. J. Basic Appl. Sci. 2011, 19, 208–212. [Google Scholar]
  41. Mnkeni, A.P.; Masika, P.; Maphaha, M. Nutritional Quality of Vegetable and Seed from Different Accessions of Amaranthus in South Africa. Water Afr. 2007, 33, 377–380. [Google Scholar] [CrossRef] [Green Version]
  42. Makobo, N.D.; Shoko, M.D.; Mtaita, T.A. Nutrient Content of Vegetable Amaranth (Amaranthus cruentus L.) at Different Harvesting Stages. World J. Agric. Sci. 2010, 6, 285–289. [Google Scholar]
  43. Amagloh, F.K.; Nyarko, E.S. Mineral Nutrient Content of Commonly Consumed Leafy Vegetables in Northern Ghana. Afr. J. Food Agric. Nutri. Dev. 2012, 12, 6397–6408. [Google Scholar] [CrossRef]
  44. Agea, J.G.; Kimondo, J.M.; Woiso, D.A.; Obaa, B.B.; Isubikalu, P.; Okullo, J.B.L.; Teklehaimanot, Z. Nutritionally Essential Macro and Micro Minerals Contents of Fifteen Selected Leafy Wild and Semi-Wild Food Plants (WSWFPs) from Bunyoro-Kitara Kingdom, Uganda. J. Nat. Prod. Plant Resour. 2014, 4, 35–42. [Google Scholar]
  45. Abe, S.G.; Willem, S.; Patrick, O.A. Genetic Diversity of Amaranthus spp. in South Africa. S. Afr. J. Sci. 2015, 32, 39–46. [Google Scholar]
  46. Kumar, A.; Lakshman, K.; Jayaveera, K.N.; Velmurugan, C.; Manoj, B.; Sridhar, S.M. Anthelmintic Activity of Methanol Extract of Amaranthus caudatus L. Internet J. Food Saf. 2010, 12, 127–129. [Google Scholar]
  47. Kumar, A.; Lakshman, K.; Jayaveera, K.N.; Nandeesh, R.; Manoj, B.; Ranganayakulu, D. Comparative in vitro Antihelminthic Activity of Three Plants from Amaranthaceae Family. Arch. Biol. Sci. 2010, 62, 185–189. [Google Scholar] [CrossRef]
  48. Reyad-ul-Ferdous, M.; Shahjahan, D.S.; Tanvir, S.; Mukti, M. Present Biological Status of Potential Medicinal Plant of Amaranthus viridis: A comprehensive review. Am. J. Clin. Exp. Med. 2015, 3, 12–17. [Google Scholar] [CrossRef] [Green Version]
  49. De Vita, D.; Messore, A.; Toniolo, C.; Frezza, C.; Scipione, L.; Bertea, C.M.; Micera, M.; Di Sarno, V.; Madia, V.N.; Pindinello, I.; et al. Towards A New Application of Amaranth Seed Oil as an Agent Against Candida albicans. Nat. Prod. Res. 2021, 35, 4621–4626. [Google Scholar] [CrossRef]
  50. Al-Mamun, M.A.; Husna, J.; Khatun, M.; Hasan, R.; Kamruzzaman, M.; Hoque, K.M.F.; Reza, M.A.; Ferdousi, Z. Assessment of Antioxidant, Anticancer and Antimicrobial Activity of Two Vegetable Species of Amaranthus in Bangladesh. BMC Complement. Altern. Med. 2016, 16, 157. [Google Scholar] [CrossRef] [Green Version]
  51. Moyer, T.B.; Heil, L.R.; Kirkpatrick, C.L.; Goldfarb, D.; Lefever, W.A.; Parsley, N.C.; Wommack, A.J.; Hicks, L.M. PepSAVI-MS Reveals a Proline-Rich Antimicrobial Peptide in Amaranthus tricolor. J. Nat. Prod. 2019, 82, 2744–2753. [Google Scholar] [CrossRef]
  52. Lipkin, A.; Anisimova, V.; Nikonorova, A.; Babakov, A.; Krause, E.; Bienert, M.; Grishin, E.; Egorov, T. An Antimicrobial Peptide Ar-Amp from Amaranth (Amaranthus retroflexus L.) Seeds. Phytochemistry 2005, 66, 2426–2431. [Google Scholar] [CrossRef]
  53. Ahmed, S.A.; Hanif, S.; Iftkhar, T. Phytochemical Profiling with Antioxidant and Antimicrobial Screening of Amaranthus viridis L. Leaf and Seed Extracts. Open J. Med. Microbiol. 2013, 3, 16–171. [Google Scholar] [CrossRef] [Green Version]
  54. Guo, L.; Wang, Y.; Bi, X.; Duo, K.; Sun, Q.; Yun, X.; Zhang, Y.; Fei, P.; Han, J. Antimicrobial Activity and Mechanism of Action of the Amaranthus tricolor Crude Extract Against Staphylococcus aureus and Potential Application in Cooked Meat. Foods 2020, 9, 359. [Google Scholar] [CrossRef] [Green Version]
  55. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Toxicity and Antimicrobial Activities of Amaranthus caudatus L. (Amaranthaceae) Harvested from Formulated Soils at Different Growth Stages. J. Evid. Based Complement. Altern. Med. 2020, 25, 2515690X20971578. [Google Scholar] [CrossRef]
  56. Maiyo, Z.C.; Ngure, R.N.; Matasyoh, J.C.; Chepkorir, R. Phytochemical Constituents and Antimicrobial Activity of Leaf Extract of Three Amaranthus Plant Species. Afr. J. Biotechnol. 2010, 9, 3178–3182. [Google Scholar]
  57. Terzieva, S.; Velichkova, K.; Grozeva, N.; Valcheva, N.; Dinev, T. Antimicrobial Activity of Amaranthus spp. Extracts Against Some Mycotoxigenic Fungi. Bulg. J. Agric. Sci. 2019, 25, 120–123. [Google Scholar]
  58. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Micromorphological Assessment of Leaves of Amaranthus caudatus L. Cultivated on Formulated Soil Types. Appl. Ecol. Environ. Res. 2019, 17, 13593–13605. [Google Scholar] [CrossRef]
  59. Antiinflamatory Lin, B.F.; Chiang, B.L.; Lin, J.Y. Amaranthus spinosus Water Extract Directly Stimulates Proliferation of B Lymphocytes in vitro. Int. Immunopharmacol. 2005, 5, 711–722. [Google Scholar]
  60. Baral, M.; Chakraborty, S.; Chakraborty, P. Evaluation of Anthelmintic and Anti-Inflammatory Activity of Amaranthus spinosus L. Int. J. Curr. Pharm. Res. 2010, 2, 2–5. [Google Scholar]
  61. Olajide, O.; Ogunleye, B.; Erinle, T. Anti-inflammatory Properties of Amaranthus spinosus Leaf Extract. Pharm. Biol. 2004, 42, 521–525. [Google Scholar] [CrossRef] [Green Version]
  62. Amornrit, W.; Santiyanont, R. Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells. Molecules 2015, 20, 17288. [Google Scholar] [CrossRef] [Green Version]
  63. House, N.C.; Puthenparampil, D.; Malayil, D.; Narayanankutty, A. Variation in The Polyphenol Composition, Antioxidant, and Anticancer Activity Among Different Amaranthus Species. S. Afr. J. Bot. 2020, 135, 408–412. [Google Scholar] [CrossRef]
  64. Jin, Y.; Xuan, Y.; Chen, M.; Chen, J.; Jin, Y.; Piao, J.; Tao, J. Antioxidant, Anti-inflammatory and Anticancer Activities of Amaranthus viridis L. Extracts. Asian J. Chem. 2013, 25, 8901–8904. [Google Scholar] [CrossRef]
  65. Sani, H.A.; Rahmat, A.; Ismail, M.; Rosli, R.; Endrini, S. Potential Anticancer Effect of Red Spinach (Amaranthus gangeticus) Extract. Asia Pac. J. Clin. Nutr. 2004, 13, 396–400. [Google Scholar] [PubMed]
  66. Zeashan, H.; Amresh, G.; Singh, S.; Rao, C.V. Hepatoprotective Activity of Amaranthus spinosus in Experimental Animals. Food Chem. Toxicol. 2008, 46, 3417–3421. [Google Scholar] [CrossRef] [PubMed]
  67. Aneja, S.; Vats, M.; Aggarwal, S.; Sardana, S. Phytochemistry and Hepatoprotective Activity of Aqueous Extract of Amaranthus tricolor L. Roots. J. Ayurveda Integr. Med. 2013, 4, 211–215. [Google Scholar] [CrossRef] [Green Version]
  68. Zeashan, H.; Amresh, G.; Singh, S.; Rao, C.V. Protective Effect of Amaranthus spinosus Against D-Galactosamine/Lipopolysaccharide-Induced Hepatic Failure. Pharm. Biol. 2010, 48, 1157–1163. [Google Scholar] [CrossRef]
  69. Zeashan, H.; Amresh, G.; Singh, S.; Rao, C.V. Hepatoprotective and Antioxidant Activity of Amaranthus spinosus Against CCl4 Induced Toxicity. J. Ethnopharmacol. 2009, 125, 364–366. [Google Scholar] [CrossRef]
  70. Allegra, M.; Tesoriere, L.; Livrea, M.A. Betanin Inhibits the Myeloperoxidase/Nitrite-Induced Oxidation of Human Low-Density Lipoproteins. Free Radic. Res. 2007, 41, 335–341. [Google Scholar] [CrossRef]
  71. Balakrishnan, S.; Pandhare, R. Antihyperglycemic and Antihyperlipidaemic Activities of Amaranthus spinosus L. Extract on Alloxan Induced Diabetic Rats. Malays. J. Pharm. Sci. 2010, 8, 13–22. [Google Scholar]
  72. Clemente, A.; Desai, P. Evaluation of the Hematological, Hypoglycemic, Hypolipidemic and Antioxidant Properties of Amaranthus tricolor Leaf Extract in Rat. Trop. J. Pharm. Res. 2011, 10, 595–602. [Google Scholar] [CrossRef] [Green Version]
  73. Yang, Y.; Fukui, R.; Jia, H.; Kato, H. Amaranth supplementation improves hepatic lipid dysmetabolism and modulates gut microbiota in mice fed a high-fat diet. Foods 2021, 10, 1259. [Google Scholar] [CrossRef]
  74. Krishnamurthy, G.; Lakshman, K.; Pruthvi, N.; Chandrika, P.U. Antihyperglicemic and Hypolipidemic Activity of Methanolic Extract of Amaranthus viridis Leaves in Experimental Diabetes. Indian J. Pharmacol. 2011, 43, 450–454. [Google Scholar]
  75. Girija, K.; Lakshman, K.; Udaya, C.; Sabhya Sachi, G.; Divya, T. Antidiabetic and Anti–cholesterolemic Activity of Methanol Extracts of Three Species of Amaranthus. Asian Pac. J. Trop. Biomed. 2011, 1, 133–138. [Google Scholar] [CrossRef] [Green Version]
  76. Hilou, A.; Nacoulma, O.G.; Guiguemde, T.R. In vivo Antimalarial Activities of Extracts from Amaranthus spinosus L. and Boerhaavia erecta L. in Mice. J. Ethnopharmacol. 2006, 103, 236–240. [Google Scholar] [CrossRef]
  77. Hsiao, L.W.; Tsay, G.J.; Mong, M.C.; Liu, W.H.; Yin, M.C. Aqueous Extract Prepared from Steamed Red Amaranth (Amaranthus gangeticus L.) Leaves Protected Human Lens Cells Against High Glucose Induced Glycative and Oxidative Stress. J. Food Sci. 2021, 86, 3686–3697. [Google Scholar] [CrossRef]
  78. Chang, Y.J.; Pong, L.Y.; Hassan, S.S.; Choo, W.S. Antiviral Activity of Betacyanins from Red Pitahaya (Hylocereus polyrhizus) and Red Spinach (Amaranthus dubius) Against Dengue Virus Type 2 (GenBank accession no. MH488959). Access Microbiol. 2020, 2, 1–6. [Google Scholar] [CrossRef]
  79. Amornrit, W.; Santiyanont, R. Neuroprotective Effect of Amaranthus lividus and Amaranthus tricolor and Their Effects on Gene Expression of RAGE During Oxidative Stress in SH-SY5Y Cells. Genet. Mol. Res. 2016, 15, gmr15027562. [Google Scholar] [CrossRef]
  80. Hussain, Z.; Amresh, G.; Singh, S.; Rao, C.V. Antidiarrheal and Antiulcer Activity of Amaranthus spinosus in Experimental Animals. Pharm. Biol. 2009, 47, 932–939. [Google Scholar] [CrossRef] [Green Version]
  81. Prajitha, V.; Thoppil, J.E. Cytotoxic and Apoptotic Activities of Extract of Amaranthus spinosus L. in Allium cepa and Human Erythrocytes. Cytotechnology 2017, 69, 123–133. [Google Scholar] [CrossRef] [Green Version]
  82. Kusumaningtyas, R.; Kobayashi, S.; Takeda, S. Mixed Species Gardens in Java and the Transmigration Areas of Sumatra, Indonesia: A Comparison. J. Tropical Agric. 2006, 44, 15–22. [Google Scholar]
  83. Vardhana, H. In vitro Antibacterial Activity of Amaranthus spinosus Root Extracts. Pharmacophore 2011, 2, 266–270. [Google Scholar]
  84. Kumar, A.; Lakshman, K.; Velmurugan, C.; Sridhar, S.M.; Gopisetty, S. Antidepressant Activity of Methanolic Extract of Amaranthus spinosus. Basic Clin. Neurosci. 2014, 5, 11–17. [Google Scholar]
  85. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Therapeutic uses of Amaranthus caudatus L. Trop. Biomed. 2019, 36, 1038–1053. [Google Scholar]
  86. Cai, Y.; Corke, H. Amaranthus Betacyanin Pigments Applied in Model Food Systems. J. Food Sci. 1999, 64, 869–873. [Google Scholar] [CrossRef]
  87. Cai, Y.; Sun, M.; Corke, H. Identification and Distribution of Simple and Acylated Betacyanins in the Amaranthaceae. J. Agric. Food Chem. 2001, 49, 1971–1978. [Google Scholar] [CrossRef]
  88. Cai, Y.; Sun, M.; Corke, H. HPLC Characterization of Betalains from Plants in the Amaranthaceae. J. Chromatogr. Sci. 2005, 43, 454–460. [Google Scholar] [CrossRef] [Green Version]
  89. Stintzing, F.C.; Carle, R. Betalains-Emerging Prospects for Food Scientists. Trends Food Sci. Technol. 2007, 18, 514–525. [Google Scholar] [CrossRef]
  90. Cai, Y.; Sun, M.; Wu, H.; Huang, R.; Corke, H. Characterization and Quantification of Betacyanin Pigments from Diverse Amaranthus Species. J. Agric. Food Chem. 1998, 46, 2063–2070. [Google Scholar] [CrossRef]
  91. Cai, Y.; Sun, M.; Corke, H. Characterization and Application of Betalain Pigments from Plants of the Amaranthaceae. Trends Food Sci. Technol. 2005, 16, 370–376. [Google Scholar] [CrossRef]
  92. Miguel, M.G. Betalains in Some Species of The Amaranthaceae Family: A Review. Antioxidants 2018, 7, 53. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  93. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Variability in Total Antioxidant Capacity, Antioxidant Leaf Pigments and Foliage Yield of Vegetable Amaranth. J. Integr. Agric. 2018, 17, 1145–1153. [Google Scholar] [CrossRef] [Green Version]
  94. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Antioxidant Leaf Pigments and Variability in Vegetable Amaranth. Genetika 2018, 50, 209–220. [Google Scholar] [CrossRef] [Green Version]
  95. Sarker, U.; Islam, M.T.; Rabbani, M.G.; Oba, S. Phenotypic Divergence in Vegetable Amaranth for Total Antioxidant Capacity, Antioxidant Profile, Dietary Fiber, Nutritional and Agronomic Traits. Acta Agric. Scand. Sect. B Soil Plant Sci. 2018, 68, 67–76. [Google Scholar] [CrossRef]
  96. Venskutonis, P.R.; Kraujalis, P. Nutritional Components of Amaranth Seeds and Vegetables: A Review on Composition, Properties, and Uses. Compr. Rev. Food Sci. Food Saf. 2013, 12, 381–412. [Google Scholar] [CrossRef]
  97. Repo-Carrasco-Valencia, R.; Hellstrom, J.K.; Philava, J.M.; Mattila, P.H. Flavonoids and Other Phenolic Compounds in Andean Indigenous Grains: Quinoa (Chenopodium quinoa), Kaniwa (Chenopodium pallidicaule) and Kiwicha (Amaranthus caudatus). Food Chem. 2010, 120, 128–133. [Google Scholar] [CrossRef]
  98. Belhadj Slimen, I.; Najar, T.; Abderrabba, M. Chemical and Antioxidant Properties of Betalains. J. Agric. Food Chem. 2017, 65, 675–689. [Google Scholar] [CrossRef]
  99. Esatbeyoglu, T.; Wagner, A.E.; Motafakkerazad, R.; Nakajima, Y.; Matsugo, S.; Rimbach, G. Free Radical Scavenging and Antioxidant Activity of Betanin: Electron Spin Resonance Spectroscopy Studies and Studies in Cultured Cells. Food Chem. Toxicol. 2014, 73, 119–126. [Google Scholar] [CrossRef]
  100. Gandía-Herrero, F.; Escribano, J.; García-Carmona, F. The Role of Phenolic Hydroxy Groups in the Free Radical Scavenging Activity of Betalains. J. Nat. Prod. 2009, 72, 1142–1146. [Google Scholar] [CrossRef]
  101. Gandía-Herrero, F.; Escribano, J.; García-Carmona, F. Structural Implications on Color, Fluorescence, and Antiradical Activity in Betalains. Planta 2010, 232, 449–460. [Google Scholar] [CrossRef]
  102. Gandía-Herrero, F.; Escribano, J.; García-Carmona, F. Purification and Antiradical Properties of the Structural Unit of Betalains. J. Nat. Prod. 2012, 75, 1030–1036. [Google Scholar] [CrossRef]
  103. Gandía-Herrero, F.; Escribano, J.; García-Carmona, F. Biological Activities of Plant Pigments Betalains. Crit. Rev. Food Sci. Nutr. 2016, 56, 937–945. [Google Scholar] [CrossRef]
  104. Khan, M.I. Plant Betalains: Safety, Antioxidant Activity, Clinical Efficacy, and Bioavailability. Compr. Rev. Food Sci. Food Saf. 2016, 15, 316–330. [Google Scholar] [CrossRef] [Green Version]
  105. Khan, M.I.; Giridhar, P. Plant Betalains: Chemistry and Biochemistry. Phytochemistry 2015, 117, 267–295. [Google Scholar] [CrossRef]
  106. Stintzing, F.C.; Carle, R. Functional Properties of Anthocyanins and Betalains in Plants, Food, and in Human Nutrition. Trends Food Sci. Technol. 2004, 15, 19–38. [Google Scholar] [CrossRef]
  107. Taira, J.; Tsuchida, E.; Katoh, M.C.; Uehara, M.; Ogi, T. Antioxidant Capacity of Betacyanins as Radical Scavengers for Peroxyl Radical and Nitric Oxide. Food Chem. 2015, 166, 531–536. [Google Scholar] [CrossRef]
  108. Li, H.; Deng, Z.; Liu, R.; Zhu, H.; Draves, J.; Marcone, M.; Sun, Y.; Tsao, R. Characterization of Phenolics, Betacyanins and Antioxidant Activities of The Seed, Leaf, Sprout, Flower and Stalk Extracts of Three Amaranthus species. J. Food Compos. Anal. 2015, 37, 75–81. [Google Scholar] [CrossRef]
  109. Raju, M.; Varakumar, S.; Lakshminarayana, R.; Krishnakantha, P.T.; Baskaran, V. Carotenoid Composition and Vitamin A Activity of Medicinally Important Green Leafy Vegetables. Food Chem. 2007, 101, 1598–1605. [Google Scholar] [CrossRef]
  110. Cai, Y.; Sun, M.; Corke, H. Antioxidant Activity of Betalains from Plants of The Amaranthaceae. J. Agric. Food Chem. 2003, 51, 2288–2294. [Google Scholar] [CrossRef]
  111. Sarker, U.; Lin, Y.P.; Oba, S.; Yoshioka, Y.; Ken, H. Prospects and potentials of underutilized leafy Amaranths as vegetable use for health-promotion. Plant Physiol. Biochem. 2022, 182, 104–123. [Google Scholar] [CrossRef] [PubMed]
  112. Pasko, P.; Bartoń, H.; Zagrodzki, P.; Gorinstein, S.; Fołta, M.; Zachwieja, Z. Anthocyanins, Total Polyphenols and Antioxidant Activity in Amaranth and Quinoa Seeds and Sprouts During Their Growth. Food Chem. 2009, 115, 994–998. [Google Scholar] [CrossRef]
  113. Asao, M.; Watanabe, K. Functional and Bioactive Properties of Quinoa and Amaranth. Food Sci. Technol. Res. 2010, 16, 163–168. [Google Scholar] [CrossRef] [Green Version]
  114. Barba de la Rosa, A.P.; Fomsgaard, I.S.; Laursen, B.; Mortensen, A.G.; Olvera-Martínez, L.; Silva-Sánchez, C.; Mendoza-Herrera, A.; González-Castañeda, J.; De León-Rodrígueza, A. Amaranth (Amaranthus hypochondriacus) as an Alternative Crop for Sustainable Food Production: Phenolic Acids and Flavonoids with Potential Impact on Its Nutraceutical Quality. J. Cereal Sci. 2009, 49, 117–121. [Google Scholar] [CrossRef]
  115. Peiretti, P.G.; Meineri, G.; Gai, F.; Longato, E.; Amarowicz, R. Antioxidative Activity and Phenolic Compounds of Pumpkin (Cucurbita Pepo) Seeds and Amaranth (Amaranthus caudatus) Grain Extracts. Nat. Prod. Res. 2017, 31, 2178–2182. [Google Scholar] [CrossRef]
  116. Stintzing, F.C.; Kammerer, D.; Schieber, A.; Adama, H.; Nacoulma, O.G.; Carle, R. Betacyanins and Phenolic Compounds from Amaranthus spinosus L. and Boerhavia erecta L. Z. Naturforsch. C 2004, 59, 1–8. [Google Scholar] [CrossRef]
  117. Kalinova, J.; Dadakova, E. Rutin and Total Quercetin Content in Amaranth (Amaranthus spp.). Plant Foods Hum. Nutr. 2009, 64, 68–74. [Google Scholar] [CrossRef]
  118. Tang, Y.; Xiao, Y.; Tang, Z.; Jin, W.; Wang, Y.; Chen, H.; Yao, H.; Shan, Z.; Bu, T.; Wang, X. Extraction of Polysaccharides from Amaranthus hybridus L. by Hot Water and Analysis of Their Antioxidant Activity. Peer J. 2019, 7, e7149. [Google Scholar] [CrossRef] [Green Version]
  119. Ozsoy, N.; Yilmaz, T.; Kurt, O.; Can, A.; Yanardag, R. In vitro Antioxidant Activity of Amaranthus lividus L. Food Chem. 2009, 116, 867–872. [Google Scholar] [CrossRef]
  120. Okunlola, G.O.; Jimoh, M.A.; Olatunji, O.A.; Olowolaju, E.D. Comparative Study of The Phytochemical Contents of Cochorus olitorius and Amaranthus hybridus at Different Stages of Growth Comparative Study of The Phytochemical Contents. Ann. West Univ. Timis. Ser. Biol. 2017, 20, 43–48. [Google Scholar]
  121. Sarikurkcu, C.; Sahinler, S.S.; Tepe, B. Astragalus gymnolobus, A. leporinus var. hirsutus, and A. onobrychis: Phytochemical Analysis and Biological Activity. Ind. Crops Prod. 2020, 150, 112366. [Google Scholar] [CrossRef]
  122. Tatiya, A.U.; Surana, S.J.; Khope, S.D.; Gokhale, S.B.; Sutar, M.P. Phytochemical Investigation and Immunomodulatory Activity of Amaranthus spinosus L. Indian J. Pharm. Educ. Res. 2007, 444, 337–341. [Google Scholar]
  123. Pamela, E.A.I.; Olufemi, T.A.; Yemisi, O.O.; Aduloju, O.A.; Usifo, G.A. Phytochemical Content and Antioxidant Activity of Five Grain Amaranth Species. Am. J. Food Sci. Technol. 2017, 5, 249–255. [Google Scholar]
  124. Pasko, P.; Barton, H.; Fołta, M.; Gwiżdż, J. Evaluation of Antioxidant Activity of Amaranth Amaranthus cruentus Grain and by-Products Flour, Popping, Cereal. Rocz. Pa’nstwowego Zakładu Hig. 2007, 581, 35–40. [Google Scholar]
  125. Nsimba, R.Y.; Kikuzaki, H.; Konishi, Y. Antioxidant Activity of Various Extracts and Fractions of Chenopodium quinoa and Amaranthus spp. Seeds. Food Chem. 2008, 106, 760–766. [Google Scholar] [CrossRef]
  126. Tang, Y.; Tsao, R. Phytochemicals in Quinoa and Amaranth Grains and Their Antioxidant, Anti-Inflammatory, and Potential Health Beneficial Effects: A Review. Mol. Nutr. Food Res. 2017, 61, 1600767. [Google Scholar] [CrossRef]
  127. Barku, V.Y.A.; Opoku-Boahen, Y.; Owusu-Ansah, E.; Mensah, E.F.; Barku, V.Y.A.; Opoku-Boahen, Y.; Owusu-Ansah, E.; Mensah, E.F. Antioxidant Activity and The Estimation of Total Phenolic and Flavonoid Contents of The Root Extract of Amaranthus spinosus. Asian J. Plant Sci. Res. 2013, 3, 69–74. [Google Scholar]
  128. Bulbul, I.J.; Nahar, L.; Ripa, F.A.; Haque, O. Antibacterial, Cytotoxic and Antioxidant Activity of Chloroform, N-Hexane and Ethyl Acetate Extract of Plant Amaranthus spinosus. Int. J. PharmTech Res. 2011, 33, 1675–1680. [Google Scholar]
  129. Kumar, B.S.A.; Lakshman, K.; Jayaveera, K.N.; Shekar, D.S.; Kumar, A.A.; Manoj, B. Antioxidant and Antipyretic Properties of Methanolic Extract of Amaranthus spinosus Leaves. Asian Pac. J. Trop. Med. 2010, 3, 702–706. [Google Scholar] [CrossRef] [Green Version]
  130. Ishtiaq, S.; Ahmad, M.; Hanif, U.; Akbar, S.; Kamran, S.H. Phytochemical and in-vitro Antioxidant Evaluation of Different Fractions of Amaranthus graecizan subsp. Silvestris Vill. Brenan. Asian Pac. J. Trop. Biomed. 2014, 412, 965–971. [Google Scholar] [CrossRef] [Green Version]
  131. Jimoh, M.O.; Afolayan, A.J.; Lewu, F.B. Antioxidant and Phytochemical Activities of Amaranthus caudatus L. Harvested from Different Soils at Various Growth Stages. Sci. Rep. 2019, 9, 12965. [Google Scholar] [CrossRef]
  132. Karamac, M.; Gai, F.; Longato, E.; Meineri, G.; Janiak, M.A.; Amarowicz, R.; Peiretti, P.G. Antioxidant Activity and Phenolic Composition of Amaranth (Amaranthus caudatus) During Plant Growth. Antioxidants 2019, 8, 173. [Google Scholar] [CrossRef] [Green Version]
  133. Kraujalis, P.; Venskutonis, P.R.; Kraujalienė, V.; Pukalskas, A. Antioxidant properties and preliminary evaluation of phytochemical composition of different anatomical parts of amaranth. Plant Foods Hum. Nutr. 2013, 68, 322–328. [Google Scholar] [CrossRef]
  134. Kumari, S.; Elancheran, R.; Devi, R. Phytochemical Screening, Antioxidant, Antityrosinase, And Antigenotoxic Potential of Amaranthus viridis Extract. Indian J. Pharmacol. 2018, 50, 130–138. [Google Scholar]
  135. Lopez-Mejía, O.A.; Lopez-Malo, A.; Palou, E. Antioxidant Capacity of Extracts from Amaranth Amaranthus hypochondriacus L. Seeds or Leaves. Ind. Crops Prod. 2014, 53, 55–59. [Google Scholar] [CrossRef]
  136. Lucero-Lopez, V.R.; Razzeto, G.S.; Gimenez, M.S.; Escudero, N.L. Antioxidant Properties of Amaranthus hypochondriacus Seeds and Their Effect on The Liver of Alcohol-Treated Rats. Plant Foods Hum. Nutr. 2011, 66, 157–162. [Google Scholar] [CrossRef]
  137. Salvamani, S.; Gunasekaran, B.; Shukor, M.Y.; Shaharuddin, N.A.; Sabullah, M.K.; Ahmad, S.A. Anti-HMG-CoA Reductase, Antioxidant, and Anti-Inflammatory Activities of Amaranthus viridis Leaf Extract as A Potential Treatment for Hypercholesterolemia. Evid. Based Complement. Altern. Med. 2016, 2016, 8090841. [Google Scholar] [CrossRef] [Green Version]
  138. Sandoval-Sicairos, E.S.; Milán-Noris, A.K.; Luna-Vital, D.A.; Milán-Carrillo, J.; Montoya-Rodríguez, A. Anti-Inflammatory and Antioxidant Effects of Peptides Released from Germinated Amaranth During In Vitro Simulated Gastrointestinal Digestion. Food Chem. 2021, 1, 128394. [Google Scholar] [CrossRef]
  139. Medoua, G.N.; Oldewage-Theron, W.H. Effect of Drying and Cooking on Nutritional Value and Antioxidant Capacity of Morogo (Amaranthus hybridus) A Traditional Leafy Vegetable Grown in South Africa. J. Food Sci. Technol. 2014, 51, 736–742. [Google Scholar] [CrossRef] [Green Version]
  140. Tesoriere, L.; Allegra, M.; Gentile, C.; Livrea, M.A. Betacyanins as Phenol Antioxidants. Chemistry and Mechanistic Aspects of the Lipoperoxyl Radical-Scavenging Activity in Solution and Liposomes. Free Radic. Res. 2009, 43, 706–717. [Google Scholar] [CrossRef]
  141. Repo-Carrasco-Valencia, R.; Peña, J.; Kallio, H.; Salminen, S. Dietary Fiber and Other Functional Components in Two Varieties of Crude and Extruded Kiwicha (Amaranthus caudatus). J. Cereal Sci. 2009, 49, 219–224. [Google Scholar] [CrossRef]
  142. Jo, H.J.; Chung, K.H.; Yoon, J.A.; Lee, K.J.; Song, B.C.; An, J.H. Radical Scavenging Activities of Tannin Extracted from Amaranth (Amaranthus caudatus L.). J. Microbiol. Biotechnol. 2015, 25, 795–802. [Google Scholar] [CrossRef]
  143. Subhasree, B.; Baskar, R.; Laxmi Keerthana, R.; Lijina Susan, R.; Rajasekaran, P. Evaluation of Antioxidant Potential in Selected Green Leafy Vegetables. Food Chem. 2009, 115, 1213–1220. [Google Scholar] [CrossRef]
  144. Lacatusu, I.; Arsenie, K.L.V.; Badea, G.; Popa, O.; Oprea, O.; Badea, N. New Cosmetic Formulations with Broad Photoprotective and Antioxidative Activities Designed by Amaranth and Pumpkin Seed Oils Nanocarriers. Ind. Crops Prod. 2018, 123, 424–433. [Google Scholar] [CrossRef]
  145. Steffensen, S.K.; Pedersen, H.A.; Labouriau, R.; Mortensen, A.G.; Laursen, B.; de Troiani, R.M.; Noellemeyer, E.J.; Janovska, D.; Stavelikova, H.; Taberner, A.; et al. Variation of Polyphenols and Betaines in Aerial Parts of Young, Field-Grown Amaranthus genotypes. J. Agric. Food Chem. 2011, 59, 12073–12082. [Google Scholar] [CrossRef]
  146. Niveyro, S.L.; Mortensen, A.G.; Fomsgaard, I.S.; Salvo, A. Differences Among Five Amaranth Varieties (Amaranthus spp.) Regarding Secondary Metabolites and Foliar Herbivory by Chewing Insects in The Field. Arthropod-Plant Interact. 2013, 7, 235–245. [Google Scholar] [CrossRef]
  147. Amin, I.; Norazaidah, Y.; Hainida, K.I.E. Antioxidant Activity and Phenolic Content of Raw and Blanched Amaranthus species. Food Chem. 2006, 94, 47–52. [Google Scholar] [CrossRef]
  148. Bao, X.; Han, X.; Du, G.; Wei, C.; Zhu, X.; Ren, W.; Zeng, L.; Zhang, Y. Antioxidant Activities and Immunomodulatory Effects in Mice of Betalain In Vivo. Food Sci. 2019, 40, 196–201. [Google Scholar]
  149. Conforti, F.; Statti, G.; Loizzo, M.R.; Sacchetti, G.; Poli, F.; Menichini, F. In Vitro Antioxidant Effect and Inhibition of Alpha-Amylase of Two Varieties of Amaranthus caudatus Seeds. Biol. Pharm. Bull. 2005, 28, 1098–1102. [Google Scholar] [CrossRef] [Green Version]
  150. Sarker, U.; Oba, S. Response of Nutrients, Minerals, Antioxidant Leaf Pigments, Vitamins, Polyphenol, Flavonoid and Antioxidant Activity in Selected Vegetable Amaranth under Four Soil Water Content. Food Chem. 2018, 252, 72–83. [Google Scholar] [CrossRef]
  151. Sarker, U.; Oba, S. Drought Stress Enhances Nutritional and Bioactive Compounds, Phenolic Acids and Antioxidant Capacity of Amaranthus Leafy Vegetable. BMC Plant Biol. 2018, 18, 258. [Google Scholar] [CrossRef] [Green Version]
  152. Sarker, U.; Oba, S. Drought Stress Effects on Growth, ROS Markers, Compatible Solutes, Phenolics, Flavonoids, and Antioxidant Activity in Amaranthus tricolor. Appl. Biochem. Biotechnol. 2018, 186, 999–1016. [Google Scholar] [CrossRef]
  153. Sarker, U.; Oba, S. Catalase, Superoxide Dismutase and Ascorbate-Glutathione Cycle Enzymes Confer Drought Tolerance of A. tricolor. Sci. Rep. 2018, 8, 16496. [Google Scholar] [CrossRef] [Green Version]
  154. Jamalluddin, N.; Massawe, F.J.; Mayes, S.; Ho, W.K.; Singh, A.; Symonds, R.C. Physiological Screening for Drought Tolerance Traits in Vegetable Amaranth (Amaranthus tricolor) Germplasm. Agriculture 2021, 11, 994. [Google Scholar] [CrossRef]
  155. Liu, F.; Stützel, H. Biomass Partitioning, Specific Leaf Area, and Water Use Efficiency of Vegetable Amaranth (Amaranthus spp.) in Response to Drought Stress. Sci. Hortic. 2004, 102, 15–27. [Google Scholar] [CrossRef]
  156. Bello, Z.A.; Walker, S. Evaluating AquaCrop Model for Simulating Production of Amaranthus (Amaranthus cruentus) a Leafy Vegetable, Under Irrigation and Rainfed Conditions. Agric. For. Meteorol. 2017, 247, 300–310. [Google Scholar] [CrossRef]
  157. Sedibe, M.M.; Combrink, N.J.J.; Reinten, E.Y. Leaf Yield of Amaranthus hypochondriatus L. (Imbuya), Affected by Irrigation Systems and Water Quality. S. Afr. J. Plant Soil 2013, 22, 171–174. [Google Scholar] [CrossRef] [Green Version]
  158. Sosnoskie, L.M.; Kichler, J.M.; Wallace, R.D.; Culpepper, A.S. Multiple Resistance in Palmer Amaranth to Glyphosate and Pyrithiobac Confirmed in Georgia. Weed Sci. 2011, 59, 321–325. [Google Scholar] [CrossRef] [Green Version]
  159. Sarker, U.; Oba, S. Salinity Stress Enhances Color Parameters, Bioactive Leaf Pigments, Vitamins, Polyphenols, Flavonoids and Antioxidant Activity in Selected Amaranthus Leafy Vegetables. J. Sci. Food Agric. 2019, 99, 2275–2284. [Google Scholar] [CrossRef]
  160. Sarker, U.; Oba, S. Augmentation of Leaf Color Parameters, Pigments, Vitamins, Phenolic Acids, Flavonoids and Antioxidant Activity in Selected Amaranthus tricolor under Salinity Stress. Sci. Rep. 2018, 8, 12349. [Google Scholar] [CrossRef] [Green Version]
  161. Sarker, U.; Islam, M.T.; Oba, S. Salinity Stress Accelerates Nutrients, Dietary Fiber, Minerals, Phytochemicals and Antioxidant Activity in Amaranthus tricolor Leaves. PLoS ONE 2018, 13, 0206388. [Google Scholar] [CrossRef] [Green Version]
  162. Sarker, U.; Oba, S. The Response of Salinity Stress-Induced A. tricolor to Growth, Anatomy, Physiology, Non-Enzymatic and Enzymatic Antioxidants. Front. Plant Sci. 2020, 11, 559876. [Google Scholar] [CrossRef]
  163. Omamt, E.N.; Hammes, P.S.; Robbertse, P.J. Differences in Salinity Tolerance for Growth and Water-Use Efficiency in Some Amaranth (Amaranthus spp.) Genotypes. N. Z. J. Crop Hortic. Sci. 2006, 34, 11–22. [Google Scholar] [CrossRef]
  164. FAO; IFAD; WFP. The State of Food Security in The World 2015. Meeting the 2015 International Hunger Targets: Taking Stock of Uneven Progress. 2015. Available online: http://www.fao.org/3/a-i4646e.pdf (accessed on 3 March 2020).
  165. Von Grebmer, K.; Saltzman, A.; Birol, E.; Wiesmann, D.; Prasai, N.; Yin, S.; Yohannes, Y.; Menon, P.; Thompson, J.; Sonntag, A. 2014 Global Hunger Index: The Challenge of Hidden Hunger; Welthungerhilfe: Bonn, Germany; International Food Policy Research Institute, and Concern Worldwide: Washington, DC, USA; Dublin, Ireland, 2014. [Google Scholar]
  166. Afari-Sefa, V.; Tenkouano, A.; Ojiewo, C.O.; Keatinge, J.D.H.; Hughes, J.D.A. Vegetable Breeding in Africa: Constraints, Complexity, and Contributions Toward Achieving Food and Nutritional Security. Food Secur. 2011, 4, 115–127. [Google Scholar] [CrossRef]
  167. Grosso, G.; Bei, R.; Mistretta, A.; Marventano, S.; Calabrese, G.; Masuelli, L.; Giganti, M.; Modesti, A.; Galvano, F.; Gazzolo, D. Effects of Vitamin C on Health: A Review of Evidence. Front. Biosci. 2013, 18, 1017–1029. [Google Scholar]
  168. Isabelle, M.; Lee, B.L.; Lim, M.T.; Koh, W.P.; Huang, D.; Ong, C.N. Antioxidant Activity and Profiles of Common Fruits in Singapore. Food Chem. 2010, 123, 77–84. [Google Scholar] [CrossRef]
  169. Randhawa, M.A.; Khan, A.A.; Javed, M.S.; Sajid, M.W. Green Leafy Vegetables: A health-promoting source. In Handbook of Fertility; Watson, R.R., Ed.; Academic Press: San Diego, CA, USA, 2015; pp. 205–220. [Google Scholar]
  170. Dusgupta, N.; De, B. Antioxidant Activity of Some Leafy Vegetables of India: A Comparative Study. Food Chem. 2007, 101, 471–474. [Google Scholar] [CrossRef]
  171. Steffensen, S.K.; Rinnan, A.; Mortensen, A.G.; Laursen, B.; Troiani, R.M.; Noellemeyer, E.J.; Janovská, D.; Dusekd, K.; Délano-Frier, J.P.; Tabernerf, A.; et al. Variations in the Polyphenol Content of Seeds of Field Grown Amaranthus Accessions. Food Chem. 2011, 129, 131–138. [Google Scholar] [CrossRef]
  172. Rice-Evans, C.A.; Miller, N.J.; Papanga, G. Antioxidant Properties of Phenolic Compounds. Trends Plant Sci. 1997, 2, 152–159. [Google Scholar] [CrossRef]
  173. Diversity Rajan, S.; Markose, B.L. Horticultural Science Series-6. In Propagation of Horticultural Crops; Peter, K.M.V., Ed.; New India Publishing Agency: New Delhi, India, 2007; Volume 6, pp. 110–113. [Google Scholar]
  174. Shukla, S.; Bhargava, A.; Chatterjee, A.; Singh, S.P. Selection Response in Vegetable Amaranth (A. tricolor) for Different Foliage Cuttings. J. Appl. Hortic. 2004, 6, 43–44. [Google Scholar]
  175. Shukla, S.; Singh, S.P. A Study on Genetic Variability and Selection Parameters of Amaranth. Farm Sci. J. 2003, 12, 164–166. [Google Scholar]
  176. Shukla, S.; Bhargava, A.; Chatterjee, A.; Pandey, A.C.; Kumar, A.R.A. Genetic Interrelationship among Nutritional and Quantitative Traits in the Vegetable Amaranth. Crop Breed. Appl. Biotechnol. 2010, 10, 16–22. [Google Scholar] [CrossRef] [Green Version]
  177. Srivastava, R. Assessment of Morphological Diversity of Selected Amaranthus Species. J. Glob. Biosci. 2015, 4, 3044–3048. [Google Scholar]
  178. Vyas, V.P.I.; Prajapati, B.H.; Patel, M.P.; Prajapati, R.R. Genetic Variability Study in Amaranthus (Amaranthus paniculatus L.). J. Farm. Sci. 2006, 15, 47–49. [Google Scholar]
  179. Wu, H.; Sun, M.; Yue, S.; Sun, H.; Cai, Y.; Huang, R.; Corke, H. Field Evaluation of an Amaranthus Genetic Resource Collection in China. Genet. Resour. Crop Evol. 2000, 47, 43–53. [Google Scholar] [CrossRef]
  180. Yadav, R.; Rana, J.C.; Ranjan, J.K. Analysis of Variability Parameters for Morphological and Agronomic Traits in Grain Amaranth (Amaranthus sp.) Genotypes. J. Veg. Sci. 2008, 35, 81–83. [Google Scholar]
  181. Rana, J.C.; Yadav, S.K.; Mandal, S.; Yadav, S. Genetic Divergence and Interrelationship Analysis in Grain Amaranth (Amaranthus hypochondriacus) Germplasm. Indian J. Genet. Plant Breed. 2005, 65, 99–102. [Google Scholar]
  182. Patial, M.; Chauhan, A.; Singh, K.P.; Sharma, D. Character Association and path Coefficient Analysis in Grain Amaranth (Amaranthus spp.). Int. J. Agric. Environ. Biotechnol. 2014, 7, 101–106. [Google Scholar] [CrossRef]
  183. Pamela, E.A.I.; Gbadegesin, M.A.; Orkpeh, U.; Ibitoye, D.O.; Odunola, O.A. Characterization of Grain Amaranth (Amaranthus spp.) Germplasm in South West Nigeria Using Morphological, Nutritional, and Random Amplified Polymorphic DNA (RAPD) Analysis. Resources 2016, 5, 6. [Google Scholar]
  184. Mandal, J.; Dhangrah, V.K. Screening Vegetable Amaranth under Summer Condition in Red and Lateritic Belt of West Bengal. Environ. Ecol. 2012, 30, 1430–1433. [Google Scholar]
  185. Mandal, J.; Dhangrah, V.K.; Chakravorty, S. Evaluation of Vegetable Amaranth under Hot Summer Growing Condition. HortFlora Res. Spectr. 2013, 2, 352–355. [Google Scholar]
  186. Khurana, D.S.; Singh, J.; Kaur, B. Genetic Variability, Correlation and Path Coefficient Analysis in Amaranthus. Veg. Sci. 2013, 36, 382–385. [Google Scholar]
  187. Hailu, A.F.; Lal, S.; Alameraw, S. Estimation of Association Characters in Amaranths Germplasm Accessions (Amaranthus spp.) under Mizan and Tepi Condtions, South West Ethiopia. Int. J. Res. 2015, 2, 1–25. [Google Scholar]
  188. Gerrano, A.S.; Rensburg, W.S.J.; Adebola, P.O. Agro-morphological Variability of Amaranthus Genotypes in South Africa. Acta Hort. 2014, 11, 183–187. [Google Scholar] [CrossRef]
  189. Anuja, S. Evaluation of Amaranthus Germplasm for Green Leaf Yield (Amaranthus spp.). Adv. Plant Sci. 2012, 25, 463–466. [Google Scholar]
  190. Anuja, S.; Mohideen, M.K. Genetic Diversity for Green Yield Characteristics in Vegetable Amaranthus (Amaranthus sp.). Plant Arch. 2006, 6, 615–617. [Google Scholar]
  191. Andini, R.; Yoshida, S.; Yoshida, Y.; Ohsawa, R. Amaranthus Genetic Resources in Indonesia: Morphological and Protein Content Assessment in Comparison with Worldwide Amaranths. Genet. Resour. Crop Evol. 2013, 60, 2115–2128. [Google Scholar] [CrossRef] [Green Version]
  192. Akaneme, F.I.; Ani, G.O. Morphological Assessment of Genetic Variability among Accessions of Amaranthus hybridus. World Appl. Sci. J. 2013, 28, 568–577. [Google Scholar]
  193. Shukla, S.; Bhargava, A.; Chatterjee, A.; Srivastava, A.; Singh, S.P. Estimates of Genetic Variability in Vegetable Amaranth (A. tricolor) over Different Cuttings. Hortic. Sci. 2005, 32, 60–67. [Google Scholar] [CrossRef] [Green Version]
  194. Ahammed, A.U.; Rahman, M.M.; Mian, M.A.K. Genetic variability, heritability and correlation study in stem amaranth (Amaranthus tricolor). Bangladesh J. Plant Breed. Genet. 2012, 25, 25–32. [Google Scholar] [CrossRef] [Green Version]
  195. Shukla, S.; Singh, S.P. Studies on Genetic Parameters in Vegetable Amaranth. J. Genet. Plant Breed. 2000, 54, 133–135. [Google Scholar]
  196. Sarker, U.; Oba, S. Nutritional and Bioactive Constituents and Scavenging Capacity of Radicals in Amaranthus hypochondriacus. Sci. Rep. 2020, 10, 19962. [Google Scholar] [CrossRef]
  197. Sarker, U.; Oba, S. Nutraceuticals, Phytochemicals, and Radical Quenching Ability of Selected Drought-Tolerant Advance Lines of Vegetable Amaranth. BMC Plant Biol. 2020, 20, 564. [Google Scholar] [CrossRef]
  198. Sarker, U.; Hossain, M.N.; Iqbal, M.A.; Oba, S. Bioactive Components and Radical Scavenging Activity in Selected Advance Lines of Salt-Tolerant Vegetable Amaranth. Front. Nutr. 2020, 7, 587257. [Google Scholar] [CrossRef] [PubMed]
  199. Sarker, U.; Oba, S. Antioxidant Constituents of Three Selected Red and Green Color Amaranthus Leafy Vegetable. Sci. Rep. 2019, 9, 18233. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  200. Sarker, U.; Oba, S. Leaf Pigmentation, Its Profiles and Radical Scavenging Activity in Selected Amaranthus tricolor Leafy Vegetables. Sci. Rep. 2020, 10, 18617. [Google Scholar] [CrossRef]
  201. Sarker, U.; Oba, S. Color Attributes, Betacyanin, and Carotenoid Profiles, Bioactive Components, and Radical Quenching Capacity in Selected Amaranthus gangeticus Leafy Vegetables. Sci. Rep. 2021, 11, 11559. [Google Scholar] [CrossRef] [PubMed]
  202. Jimenez-Aguilar, D.M.; Grusak, M.A. Minerals, Vitamin C, Phenolics, Flavonoids and Antioxidant Activity of Amaranthus Leafy Vegetables. J. Food Compos. Anal. 2017, 58, 33–39. [Google Scholar] [CrossRef] [Green Version]
  203. Sarker, U.; Oba, S. Phenolic Profiles and Antioxidant Activities in Selected Drought-Tolerant Leafy Vegetable Amaranth. Sci. Rep. 2020, 10, 18287. [Google Scholar] [CrossRef] [PubMed]
  204. Sarker, U.; Oba, S. Polyphenol and Flavonoid Profiles and Radical Scavenging Activity in Selected Leafy Vegetable Amaranthus gangeticus. BMC Plant Biol. 2020, 20, 499. [Google Scholar] [CrossRef]
  205. Khanam, U.K.S.; Oba, S.; Yanase, E.; Murakami, Y. Phenolic Acids, flavonoids and Total Antioxidant Capacity of Selected Leafy Vegetables. J. Funct. Foods 2012, 4, 979–987. [Google Scholar] [CrossRef]
  206. Azad, A.K.; Sarker, U.; Ercisli, S.; Assouguem, A.; Ullah, R.; Almeer, R.; Sayed, A.A.; Peluso, I. Evaluation of Combining Ability and Heterosis of Popular Restorer and Male Sterile Lines for the Development of Superior Rice Hybrids. Agronomy 2022, 12, 965. [Google Scholar] [CrossRef]
  207. Prodhan, M.M.; Sarker, U.; Hoque, M.A.; Biswas, M.S.; Ercisli, S.; Assouguem, A.; Ullah, R.; Almutairi, M.H.; Mohamed, H.R.H.; Najda, A. Foliar Application of GA3 Stimulates Seed Production in Cauliflower. Agronomy 2022, 12, 1394. [Google Scholar] [CrossRef]
  208. Sarker, U.; Azam, M.G.; Talukder, M.Z.A. Genetic Variation in Mineral Profiles, Yield Contributing Agronomic Traits, and Foliage Yield of Stem Amaranth. Genetika 2022, 54, 91–108. [Google Scholar] [CrossRef]
  209. Sun, H.; Mu, T.; Xi, L.; Zhang, M.; Chen, J. Sweet Potato (Ipomoea batatas L.) Leaves as Nutritional and Functional Foods. Food Chem. 2014, 156, 380–389. [Google Scholar] [CrossRef]
  210. Sarker, U.; Oba, S. Protein, Dietary Fiber, Minerals, Antioxidant Pigments and Phytochemicals, and Antioxidant Activity in Selected Red Morph Amaranthus Leafy Vegetable. PLoS ONE 2019, 14, 0222517. [Google Scholar] [CrossRef] [Green Version]
  211. Sarker, U.; Hossain, M.M.; Oba, S. Nutritional and Antioxidant Components and Antioxidant Capacity in Green Morph Amaranthus Leafy Vegetable. Sci. Rep. 2020, 10, 1336. [Google Scholar] [CrossRef]
  212. Sarker, U.; Oba, S. Nutraceuticals, Antioxidant Pigments, and Phytochemicals in the Leaves of Amaranthus spinosus and Amaranthus viridis Weedy Species. Sci Rep. 2019, 9, 20413. [Google Scholar] [CrossRef] [Green Version]
  213. Sarker, U.; Oba, S.; Daramy, M.A. Nutrients, Minerals, Antioxidant Pigments and Phytochemicals, and Antioxidant Capacity of the Leaves of stem amaranth. Sci. Rep. 2020, 10, 3892. [Google Scholar] [CrossRef] [Green Version]
  214. Sarker, U.; Oba, S. Nutrients, Minerals, Pigments, Phytochemical, and Radical Scavenging Activity in Amaranthus blitum Leafy Vegetable. Sci. Rep. 2020, 10, 3868. [Google Scholar] [CrossRef] [Green Version]
  215. Madruga, M.S.; Camara, F.S. The Chemical Composition of “Multimistura” as A Food Supplement. Food Chem. 2000, 68, 41–44. [Google Scholar] [CrossRef]
  216. Shahidi, F.; Chavan, U.D.; Bal, A.K.; McKenzie, D.B. Chemical Composition of Beach Pea (Lathyrus maritimus L.) Plant Parts. Food Chem. 1999, 64, 39–44. [Google Scholar] [CrossRef]
  217. Khanam, U.K.S.; Oba, S. Bioactive Substances in Leaves of Two Amaranth Species, Amaranthus lividus, and A. hypochondriacus. Can. J. Plant. Sci. 2013, 93, 47–58. [Google Scholar] [CrossRef]
  218. Alam, M.A.; Juraimi, A.S.; Rafii, M.Y.; Hamid, A.A.; Aslani, F.; Alam, M.Z. Effects of Salinity and Salinity-Induced Augmented Bioactive Compounds in Purslane (Portulaca oleracea L.) for Possible Economical Use. Food Chem. 2015, 169, 439–447. [Google Scholar] [CrossRef] [PubMed]
  219. Hossain, M.N.; Sarker, U.; Raihan, M.S.; Al-Huqail, A.A.; Siddiqui, M.H.; Oba, S. Influence of Salinity Stress on Color Parameters, Leaf Pigmentation, Polyphenol and Flavonoid Contents, and Antioxidant Activity of Amaranthus lividus Leafy Vegetables. Molecules 2022, 27, 1821. [Google Scholar] [CrossRef] [PubMed]
  220. Sarker, U.; Oba, S.; Ercisli, S.; Assouguem, A.; Alotaibi, A.; Ullah, R. Bioactive Phytochemicals and Quenching Activity of Radicals in Selected Drought-Resistant Amaranthus tricolor Vegetable Amaranth. Antioxidants 2022, 11, 578. [Google Scholar] [CrossRef] [PubMed]
  221. Sarker, U.; Rabbani, M.G.; Oba, S.; Eldehna, W.M.; Al-Rashood, S.T.; Mostafa, N.M.; Eldahshan, O.A. Phytonutrients, Colorant Pigments, Phytochemicals, and Antioxidant Potential of Orphan Leafy Amaranthus Species. Molecules 2022, 27, 2899. [Google Scholar] [CrossRef]
  222. Sarker, U.; Oba, S.; Alsanie, W.F.; Gaber, A. Characterization of Phytochemicals, Nutrients, and Antiradical Potential in Slim Amaranth. Antioxidants 2022, 11, 1089. [Google Scholar] [CrossRef]
Figure 1. Proximate composition (per 100 g−1 FW, energy kcal 100 g−1 FW) in danta leaves. (A) Moisture and energy, (B) Protein, fat, carbohydrates, ash and digestive fiber. Dissimilar letters over the bars indicate that the corresponding data significantly differed by Duncan multiple range test (DMRT) (p < 0.01, n = 6).
Figure 1. Proximate composition (per 100 g−1 FW, energy kcal 100 g−1 FW) in danta leaves. (A) Moisture and energy, (B) Protein, fat, carbohydrates, ash and digestive fiber. Dissimilar letters over the bars indicate that the corresponding data significantly differed by Duncan multiple range test (DMRT) (p < 0.01, n = 6).
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Figure 2. Mineral elements (macroelements mg g−1 FW, microelements µg g−1 FW) in danta leaves. (A) Iron, zinc and manganese, (B) Potassium, calcium, magnesium, and copper. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01, n = 6).
Figure 2. Mineral elements (macroelements mg g−1 FW, microelements µg g−1 FW) in danta leaves. (A) Iron, zinc and manganese, (B) Potassium, calcium, magnesium, and copper. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01, n = 6).
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Figure 3. Bioactive pigments in danta leaves. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01, n = 6).
Figure 3. Bioactive pigments in danta leaves. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01, n = 6).
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Figure 4. Bioactive components and AP in danta leaves. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01); AP, antiradical potential; TF, total flavonoids; TP, total polyphenols (n = 6).
Figure 4. Bioactive components and AP in danta leaves. Dissimilar letters over the bars indicate that the corresponding data significantly differed by DMRT (p < 0.01); AP, antiradical potential; TF, total flavonoids; TP, total polyphenols (n = 6).
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Table 1. The correlation coefficient for ascorbic acid, pigments, TP, β-carotene, AP (DPPH), TF, and AP (ABTS+) in danta leaves.
Table 1. The correlation coefficient for ascorbic acid, pigments, TP, β-carotene, AP (DPPH), TF, and AP (ABTS+) in danta leaves.
CharactersChbChabBBnBlβCAsATPTFAPAP
Cha0.92 **0.98 **0.94 **0.95 **0.92 **−0.82 *−0.0240.98 **0.87 *0.84 *0.83 *
Chb 0.95 **0.93 **0.92 **0.96 **−0.71−0.0230.81 *0.85 *0.85 *0.87 *
Chab 0.82 *0.84 *0.93 **−0.85 *−0.0220.86 *0.88 *0.86 *0.86 *
B 0.97 **0.98 **−0.81 *−0.1240.85 *0.82 *0.98 **0.98 **
Bn 0.97 **−0.87 **−0.1350.83 *0.81 *0.85 *0.92 **
Bl −0.94 **−0.1180.95 **0.86 *0.97 **0.95 **
βC 0.77 *0.96 **0.94 **0.92 **0.97 **
AsA 0.86 *0.95 **0.97 **0.82 *
TP 0.95 **0.98 **0.98 **
TF 0.87 *0.99 **
AP (DPPH) 0.96 **
Chb, chlorophyll b; Cha, chlorophyll a; B, betacyanins; Chab, chlorophyll ab; Bl, betalains; Bn, betaxanthins; βC, β-carotene; AsA, ascorbic acid; TF, total flavonoids; AP, antiradical potential; TP, total polyphenols; *, ** significant at the 5% and 1% levels, respectively.
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Sarker, U.; Iqbal, M.A.; Hossain, M.N.; Oba, S.; Ercisli, S.; Muresan, C.C.; Marc, R.A. Colorant Pigments, Nutrients, Bioactive Components, and Antiradical Potential of Danta Leaves (Amaranthus lividus). Antioxidants 2022, 11, 1206. https://doi.org/10.3390/antiox11061206

AMA Style

Sarker U, Iqbal MA, Hossain MN, Oba S, Ercisli S, Muresan CC, Marc RA. Colorant Pigments, Nutrients, Bioactive Components, and Antiradical Potential of Danta Leaves (Amaranthus lividus). Antioxidants. 2022; 11(6):1206. https://doi.org/10.3390/antiox11061206

Chicago/Turabian Style

Sarker, Umakanta, Md. Asif Iqbal, Md. Nazmul Hossain, Shinya Oba, Sezai Ercisli, Crina Carmen Muresan, and Romina Alina Marc. 2022. "Colorant Pigments, Nutrients, Bioactive Components, and Antiradical Potential of Danta Leaves (Amaranthus lividus)" Antioxidants 11, no. 6: 1206. https://doi.org/10.3390/antiox11061206

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