Surveys on Exposure to Reptile-Associated Salmonellosis (RAS) in the Piedmont Region—Italy
2. Materials and Methods
2.1. Prevalence of RAS among Salmonellosis Cases
2.2. Evaluation of Exposure to RAS and of People’s Awareness
2.2.1. Evaluation of the Human Exposure in Public Places Displaying Reptiles
2.2.2. Evaluation of People’s Awareness about RAS
2.2.3. Statistical Analysis
3.1. Prevalence of RAS among Salmonellosis Cases
- Case 1: the patient did not agree to participate in the more extensive questionnaire;
- Case 2: the animal’s swabs resulted negative, and the patient was exposed to other risk factors;
- Case 3: the patient was exposed to other risk factors;
- Case 4: the samples both from the animal and the patient tested positive during analysis, but PFGE showed that different strains were involved (S. enterica ser. Lome for the animal and another not identified variant for the boy).
- Case 1: a 14-year-old child had contact with a marsh turtle hosted at home. The aquarium was washed and cleaned directly in the bathtub. S. enterica ser. Typhimurium monophasic variant (4, , 12:i:-) was isolated from environmental swabs, and PFGE confirmed the identity of the strain isolated in the patient;
- Case 2: a 2-year-old child had contact with a marsh turtle hosted at home. The family used to throw the water and clean the aquaterrarium in the sink of the kitchen. No other risk factors were present. S. enterica ser. Pomona was isolated from swabs obtained from the animal and the water of the aquaterrarium. The isolate from the patient could not be retrieved for further investigation, and the serovar was not determined at the hospital where the diagnosis was made;
- Case 3 and 4: a 7-year-old boy and a 10-year-old girl, both had contact with pet marsh turtles. The aquariums were cleaned in the garden and the kitchen sink, respectively. All samplings were negative, but cases were recognized as RAS considering the possible intermittent elimination of Salmonella by reptiles and the absence of other risk factors;
- Case 5: a 16-year-old boy had contact with a marsh turtle hosted at home, but he did not agree to answer the more extensive questionnaire. The SIAN doctor declared the absence of other risk factors; therefore, the episode was considered RAS.
3.2. Evaluation of Exposure to RAS and of People’s Awareness
3.2.1. Evaluation of the Human Exposure in Public Places Displaying Reptiles
3.2.2. Evaluation of People’s Awareness about RAS
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Meletiadis, A.; Biolatti, C.; Mugetti, D.; Zaccaria, T.; Cipriani, R.; Pitti, M.; Decastelli, L.; Cimino, F.; Dondo, A.; Maurella, C.; et al. Surveys on Exposure to Reptile-Associated Salmonellosis (RAS) in the Piedmont Region—Italy. Animals 2022, 12, 906. https://doi.org/10.3390/ani12070906
Meletiadis A, Biolatti C, Mugetti D, Zaccaria T, Cipriani R, Pitti M, Decastelli L, Cimino F, Dondo A, Maurella C, et al. Surveys on Exposure to Reptile-Associated Salmonellosis (RAS) in the Piedmont Region—Italy. Animals. 2022; 12(7):906. https://doi.org/10.3390/ani12070906Chicago/Turabian Style
Meletiadis, Arianna, Cristina Biolatti, Davide Mugetti, Teresa Zaccaria, Raffaella Cipriani, Monica Pitti, Lucia Decastelli, Francesca Cimino, Alessandro Dondo, Cristiana Maurella, and et al. 2022. "Surveys on Exposure to Reptile-Associated Salmonellosis (RAS) in the Piedmont Region—Italy" Animals 12, no. 7: 906. https://doi.org/10.3390/ani12070906