Animals

7 pages, 254 KiB

Open AccessArticle

Effect of the In Ovo Injection Site of Electrolytes on Some Biochemical Blood Parameters and Quality of Layer Chicks

by Joanna Pawłowska, Ewa Sosnówka-Czajka and Iwona Skomorucha

Animals 2022, 12(4), 532; https://doi.org/10.3390/ani12040532 - 21 Feb 2022

Cited by 1 | Viewed by 1778

The effects of the in ovo injection site of electrolytes on selected biochemical blood parameters and the quality of layer chicks were investigated. A total of 120 fertile eggs from Rhode Island Red breeders were randomly distributed into 4 groups, with each group [...] Read more.

The effects of the in ovo injection site of electrolytes on selected biochemical blood parameters and the quality of layer chicks were investigated. A total of 120 fertile eggs from Rhode Island Red breeders were randomly distributed into 4 groups, with each group including 30 birds. The groups were as follows: untreated control and groups with different injection sites/locations of 500 µL of 0.9% saline (NaCl) on day 18 of incubation, i.e., into the air cell (AC), through the air cell into the amniotic fluid (AFA), and directly into the amniotic fluid (AF). Measurement at 1 day of age showed that regardless of the injection site, embryos injected with 500 µL of saline had significantly higher Tona score (95/100 points) compared to the control group (90/100 points). Chick length was similar among the injected groups (mean 14.7 cm) and shorter in the control group (13.9 cm). There was no significant effect of in ovo injection on the biochemical blood parameters: total protein, cholesterol, high-density lipoprotein, low-density lipoprotein, glucose, urea, and uric acid. The highest concentration of sodium was noted in the control group (141.59 mmol/L). Regardless of the injection site/location, chicks treated with 500 µL of NaCl were characterized by a significantly lower blood sodium concentration (by 7.45% (AC), 7.90% (AFA), and 4.84% (AF) compared with birds from the control group (p ≤ 0.01)). The influence of saline solution administration in ovo on the blood potassium content of chicks was demonstrated. The concentration of potassium in the control group was significantly higher (by 11.36%) than in the AC group (p ≤ 0.01). In conclusion, the injection of 500 µL of saline solution into the developing chick embryo during the last days of incubation may have a positive effect on the quality of day-old chicks. Full article

(This article belongs to the Section Poultry)

11 pages, 896 KiB

Open AccessArticle

Temperature and Diet Acclimation Modify the Acute Thermal Performance of the Largest Extant Amphibian

by Chun-Lin Zhao, Tian Zhao, Jian-Yi Feng, Li-Ming Chang, Pu-Yang Zheng, Shi-Jian Fu, Xiu-Ming Li, Bi-Song Yue, Jian-Ping Jiang and Wei Zhu

Animals 2022, 12(4), 531; https://doi.org/10.3390/ani12040531 - 21 Feb 2022

Cited by 4 | Viewed by 2636

Abstract

The Chinese giant salamander (Andrias davidianus), one of the largest extant amphibian species, has dramatically declined in the wild. As an ectotherm, it may be further threatened by climate change. Therefore, understanding the thermal physiology of this species should be the [...] Read more.

The Chinese giant salamander (Andrias davidianus), one of the largest extant amphibian species, has dramatically declined in the wild. As an ectotherm, it may be further threatened by climate change. Therefore, understanding the thermal physiology of this species should be the priority to formulate related conservation strategies. In this study, the plasticity in metabolic rate and thermal tolerance limits of A. davidianus larvae were studied. Specifically, the larvae were acclimated to three temperature levels (7 °C, cold stress; 15 °C, optimum; and 25 °C, heat stress) and two diet items (red worm or fish fray) for 20 days. Our results indicated that cold-acclimated larvae showed increased metabolic capacity, while warm-acclimated larvae showed a decrease in metabolic capacity. These results suggested the existence of thermal compensation. Moreover, the thermal tolerance windows of cold-acclimated and warm-acclimated larvae shifted to cooler and hotter ranges, respectively. Metabolic capacity is not affected by diet but fish-fed larvae showed superiority in both cold and heat tolerance, potentially due to the input of greater nutrient loads. Overall, our results suggested a plastic thermal tolerance of A. davidianus in response to temperature and diet variations. These results are meaningful in guiding the conservation of this species. Full article

(This article belongs to the Special Issue Salamanders: Distribution, Diversity, and Conservation)

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16 pages, 2327 KiB

Open AccessArticle

The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes

by Tania García-Martínez, Iris Martínez-Rodero, Joan Roncero-Carol, Meritxell Vendrell-Flotats, Jaume Gardela, Alfonso Gutiérrez-Adán, Priscila Ramos-Ibeas, Adam Z. Higgins and Teresa Mogas

Animals 2022, 12(4), 530; https://doi.org/10.3390/ani12040530 - 21 Feb 2022

Cited by 1 | Viewed by 2385

Abstract

Aquaglyceroporins are known as channel proteins, and are able to transport water and small neutral solutes. In this study, we evaluate the effect of exposure of in vitro matured bovine oocytes to hyperosmotic solutions containing ethylene glycol (EG), dimethyl sulfoxide (Me₂SO) [...] Read more.

Aquaglyceroporins are known as channel proteins, and are able to transport water and small neutral solutes. In this study, we evaluate the effect of exposure of in vitro matured bovine oocytes to hyperosmotic solutions containing ethylene glycol (EG), dimethyl sulfoxide (Me₂SO) or sucrose on the expression levels of AQP3, AQP7 and AQP9. Moreover, we studied whether artificial protein expression of AQP7 in bovine oocytes increases their permeability to water and cryoprotectants. Exposure to hyperosmotic solutions stimulated AQP3 and AQP7 but not AQP9 expression. Oocytes exposed to hyperosmotic Me₂SO solution exhibited upregulated AQP3 expression, while AQP7 expression was upregulated by EG hyperosmotic exposure. Microinjection of oocytes at the germinal vesicle stage with enhanced green fluorescent protein (EGFP) or EGFP+AQP7 cRNAs resulted in the expression of the corresponding proteins in ≈86% of the metaphase-II stage oocytes. AQP7 facilitated water diffusion when bovine MII oocytes were in presence of Me₂SO solution but not EG or sucrose solution. However, the overexpression of this aquaporin did not increase membrane permeability to Me₂SO or EG. In summary, cryoprotectant-induced increase of AQP3 and AQP7 expression could be one of the mechanisms underlying oocyte tolerance to hyperosmotic stress. Water diffusion appears to be improved when AQP7 overexpressed oocytes are exposed to Me₂SO, shortening the time required for oocytes to achieve osmotic balance with cryoprotectant solutions. Full article

(This article belongs to the Special Issue New Challenges in Cryopreservation)

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10 pages, 1410 KiB

Open AccessArticle

Logistic Regression Model for Determination of the Age of Brown Hare (Lepus europaeus Pall.) Based on Body Weight

by Marian Flis, Piotr Czyżowski, Sławomir Beeger, Bogusław Rataj and Mirosław Karpiński

Animals 2022, 12(4), 529; https://doi.org/10.3390/ani12040529 - 21 Feb 2022

Cited by 3 | Viewed by 1597

Abstract

We developed an algorithm to classify brown hares into two age classes, juveniles (up to 1 year old) and adults (over 1 year old), based on body weight, which can be determined by both the examination of live animals and postmortem analysis. Considering [...] Read more.

We developed an algorithm to classify brown hares into two age classes, juveniles (up to 1 year old) and adults (over 1 year old), based on body weight, which can be determined by both the examination of live animals and postmortem analysis. Considering the strong correlation between lens weight and carcass weight, we assumed that hares could be classified into one of the two age groups based only on carcass weight, using a logistic regression model. Using logistic regression, a model was constructed to assess the age of hares based on their body weight. For comparison with the current age-assessment method based on the dry lens weight, a logistic regression classifying the hares based on the dry lens weight was performed as well. The results of the study facilitated the development of a method to classify hares into age groups based on body weight. The proposed approach is innovative, as it allows for the determination of the age of not only culled (postmortem) but also live hares. The method is easy and does not require laboratory tests; hence, the results can be used immediately following evaluation. This method allows hares to be categorized into two age groups (juveniles and adults). With an accuracy of 97.52% and 95.45% in the case of juvenile and adult hares, respectively, the proposed approach can be widely used both in population management and scientific research. Full article

(This article belongs to the Section Wildlife)

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10 pages, 895 KiB

Open AccessArticle

Limiting Dietary Lysine Increases Body Weight Variability by Restricting Growth Potential of the Lightest Growing Pigs

by Pau Aymerich, Carme Soldevila, Jordi Bonet, Josep Gasa, Jaume Coma and David Solà-Oriol

Animals 2022, 12(4), 528; https://doi.org/10.3390/ani12040528 - 21 Feb 2022

Cited by 1 | Viewed by 1600

Abstract

The goal of this experiment was to determine the implications of dietary standardized ileal digestible lysine (SID Lys:NE) on body weight variability of growing pigs grouped in three initial body weight categories (BWCAT). Animals (N = 1170) were individually weighed and classified [...] Read more.

The goal of this experiment was to determine the implications of dietary standardized ileal digestible lysine (SID Lys:NE) on body weight variability of growing pigs grouped in three initial body weight categories (BWCAT). Animals (N = 1170) were individually weighed and classified in 3 BWCAT (Lp: 32.1 ± 2.8 kg, Mp: 27.5 ± 2.3 kg, and Sp: 23.4 ± 2.9 kg). Afterward, pens were randomly allocated to five dietary SID Lys:NE treatments (3.25 to 4.88 g/Mcal) that were fed over 47 days. Pen coefficient of variation of Sp at day 47 was linearly increased when reducing SID Lys:NE (p < 0.01), from 9.8% to 15.4% between the two extreme dietary levels. The linear effect was different in Sp compared to Lp (p < 0.05) as no effect of SID Lys:NE was reported in the latter BWCAT (p = 0.992). Further analysis showed that this effect was explained by a growth restriction that was more severe the lightest the pigs were at the start of the trial. To summarize, swine body weight variability can be negatively affected when SID Lys requirements are not fulfilled. Full article

(This article belongs to the Special Issue Impact of Body Weight Variability in the Swine Industry: A Holistic Approach)

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12 pages, 1947 KiB

Open AccessCommunication

A Virtual Necropsy: Applications of 3D Scanning for Marine Mammal Pathology and Education

by Ellen M. Chenoweth, Josh Houston, Kathy Burek Huntington and Jan M. Straley

Animals 2022, 12(4), 527; https://doi.org/10.3390/ani12040527 - 21 Feb 2022

Cited by 5 | Viewed by 3311

Abstract

Stranded large whales represent an opportunity to learn about the anatomy and health of these cryptic free-ranging animals. However, where time and access is frequently limited, law enforcement and management priorities often take precedence over research, outreach, and educational uses. On 14 March [...] Read more.

Stranded large whales represent an opportunity to learn about the anatomy and health of these cryptic free-ranging animals. However, where time and access is frequently limited, law enforcement and management priorities often take precedence over research, outreach, and educational uses. On 14 March 2021, a dead female adult humpback whale was reported stranded on an uninhabited island 15 miles west of Sitka, Alaska. The whale was three-dimensionally scanned using light detection and ranging (LiDAR) and photogrammetry before, during, and at multiple time points after a necropsy, including full decomposition 17 days later (NOAA Fisheries permit 18786-01). These scans were organized and displayed on the site Sketchfab with annotations and made publically available as a “4D virtual necropsy” (the fourth dimension is time). After one month, our user survey indicated widespread interest in the platform by both the local community and worldwide by stranding professionals, researchers, and educators. We are unaware of another 3D scan involving a large whale with soft tissue for teaching, research, or public display, despite the ease of 3D scanning with current technologies and the wide-ranging applications. Full article

(This article belongs to the Special Issue Pathology of Marine Mammals)

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14 pages, 1359 KiB

Open AccessArticle

Comparison of Flunixin Meglumine, Meloxicam and Ketoprofen on Mild Visceral Post-Operative Pain in Horses

by Louise C. Lemonnier, Chantal Thorin, Antoine Meurice, Alice Dubus, Gwenola Touzot-Jourde, Anne Couroucé and Aurélia A. Leroux

Animals 2022, 12(4), 526; https://doi.org/10.3390/ani12040526 - 21 Feb 2022

Cited by 6 | Viewed by 3571

Abstract

The analgesic efficacy of meloxicam and ketoprofen against equine visceral pain is unclear. The aim of this study was to compare the analgesic efficacy of meloxicam (M) and ketoprofen (K) to flunixin meglumine (F) following inguinal castration. Horses undergoing inguinal castration under general [...] Read more.

The analgesic efficacy of meloxicam and ketoprofen against equine visceral pain is unclear. The aim of this study was to compare the analgesic efficacy of meloxicam (M) and ketoprofen (K) to flunixin meglumine (F) following inguinal castration. Horses undergoing inguinal castration under general anesthesia were randomly assigned F (1.1 mg/kg), M (0.6 mg/kg) or K (2.2 mg/kg) intravenously two hours pre-operatively and 24 h later. A pain score (out of 31) was recorded blindly by a senior clinician and veterinary student before NSAIDs administration (T₀), and after the first (T₁) and second (T₂) administrations, using a modified post-abdominal surgery pain assessment scale (PASPAS). Pain was classified as mild (score

\leq

7), moderate (score = 8–14) or severe (score

>

14). Thirty horses (12 F, 10 M, 8 K) aged 6.2

\pm

4.9 years, mostly warmbloods, were included. Horse welfare was not compromised regardless of the drug assigned. There was no statistically significant effect of NSAIDs on pain score. Mean pain scores were significantly higher at T₁ than T₀ for each NSAID (F: 5.08 ± 2.50 vs. 1.58 ± 1.38 (p < 0.001); M: 4.60 ± 2.32 vs. 1.10 ± 1.20 (p < 0.001); K: 5.25 ± 1.39 vs. 1.50 ± 1.51 (p < 0.0001)) and lower at T₂ than T₁ for F (2.92 ± 2.423 vs. 5.08 ± 2.50 (p < 0.001)) and M (2.90 ± 1.37 vs. 4.60 ± 2.32 (p < 0.0325)). At T₁, senior pain scores were significantly different than for junior (5.56 ± 0.54 vs. 3.22 ± 0.62, p = 0.005). This study indicates that meloxicam and ketoprofen provide a similar level of analgesia to flunixin meglumine for the management of mild visceral pain in horses. PASPAS is not reliable for junior evaluators. Full article

(This article belongs to the Section Equids)

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11 pages, 449 KiB

Open AccessArticle

Effect of Different Water Cooling Treatments on Changes in Rectal and Surface Body Temperature in Leisure Horses after Medium-Intensity Effort

by Iwona Janczarek, Anna Wiśniewska, Ewelina Tkaczyk, Elżbieta Wnuk-Pawlak, Beata Kaczmarek, Marta Liss-Szczepanek and Witold Kędzierski

Animals 2022, 12(4), 525; https://doi.org/10.3390/ani12040525 - 21 Feb 2022

Cited by 6 | Viewed by 3044

Abstract

Cooling a horse after intensive exercise under hot conditions is commonly recommended. The study aimed to analyze changes in the rectal and surface temperature of the horses subjected to various water cooling treatments. This followed medium-intensity exercise performed by leisure horses under moderate [...] Read more.

Cooling a horse after intensive exercise under hot conditions is commonly recommended. The study aimed to analyze changes in the rectal and surface temperature of the horses subjected to various water cooling treatments. This followed medium-intensity exercise performed by leisure horses under moderate air temperature. The experiment involved a control group without water application, and three variants of water cooling applied to 19 warmblood geldings after medium-intensity effort. Cooling of lower, upper, and lower and upper body parts was performed. In each variant, the rectal and body surface temperatures were measured five times: before; immediately after; and 10, 20, and 30 min after effort. Using water cooling under the studied conditions did not influence a post-exercise decrease in the rectal temperature. The decrease in body surface temperature depended on the used variant of cooling down the horse. Cooling the limbs by pouring water several times changed the surface body temperature from 34.2 ± 0.37 °C to 32.0 ± 0.32 °C and was more efficient than the repeated application of cool water on both the upper and lower body parts, leading to a temperature change from 34.6 ± 0.26 °C to 33.2 ± 0.36 °C. Thus, the application of cold water on the limbs only is sufficient for cooling the horse after medium-intensity exercise under moderate air temperature (about 24 °C). Full article

(This article belongs to the Special Issue Thermal stress—Thermoregulatory and Adaptive Responses of Livestock)

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15 pages, 1208 KiB

Open AccessArticle

A Descriptive Study of the Clinical Presentation, Management, and Outcome of Horses with Acute Soft Tissue Trauma of the Tarsus and the Association with Synovial Involvement

by Taja Vajs, Omid Nekouei and Nora M. Biermann

Animals 2022, 12(4), 524; https://doi.org/10.3390/ani12040524 - 21 Feb 2022

Cited by 3 | Viewed by 4326

Abstract

The tarsus is one of the most common areas of traumatic injury with associated synovial involvement (SI) in horses. The aim of this retrospective study was to describe the clinical presentation, diagnostic procedures, management (emphasizing the type, duration, and route of antimicrobial administration), [...] Read more.

The tarsus is one of the most common areas of traumatic injury with associated synovial involvement (SI) in horses. The aim of this retrospective study was to describe the clinical presentation, diagnostic procedures, management (emphasizing the type, duration, and route of antimicrobial administration), and outcome of cases with acute soft tissue trauma to the tarsal region. The presenting clinical features, the results of diagnostic modalities, and the initial response to therapy were assessed for their usefulness to predict SI. Medical records of 72 cases were included and SI was diagnosed in 34 cases (47.2%). Increased synovial effusion, lameness on admission (OR = 4.1; 95%CI 1.0–16.4), persistent lameness (OR = 5.7; 95%CI 1.8–17.9), increased blood SAA values (≥200 mg/L) from initial to second measurement (OR = 4.3; 95%CI 1.2–15.5), and wound location on the plantar/plantarolateral/plantaromedial compared to the lateral aspect of the tarsus (OR = 7.0; 95%CI 1.6–30.9) were associated with SI. Radiographs, ultrasonography, and the use of pressure testing when a wound was present proved to be useful in correctly diagnosing SI. The median duration of systemic antimicrobial administration was 8 (IQR: 5 to 9) days and most horses received local antimicrobial therapy. This study highlights several relevant clinical features and their association with SI and emphasizes the usefulness of local antimicrobial therapy in these cases. Full article

(This article belongs to the Special Issue Horse Surgery)

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17 pages, 1956 KiB

Open AccessArticle

Feeding System Effects on Dairy Cow Rumen Function and Milk Production

by Stephen McAuliffe, John F. Mee, Eva Lewis, Norann Galvin and Deirdre Hennessy

Animals 2022, 12(4), 523; https://doi.org/10.3390/ani12040523 - 21 Feb 2022

Cited by 3 | Viewed by 2773

Abstract

Good rumen function, which is largely influenced by the diet of the cow, is essential to optimise animal performance. This study, conducted over the course of a full lactation in a spring-calving milk production system, compared the rumen function and milk production of [...] Read more.

Good rumen function, which is largely influenced by the diet of the cow, is essential to optimise animal performance. This study, conducted over the course of a full lactation in a spring-calving milk production system, compared the rumen function and milk production of cows offered one of three dietary treatments: (1) Cows grazing grass-only swards receiving 250 kg nitrogen (N)/ha/year (Grass), (2) Cows grazing grass-white clover swards receiving 250 kg N/ha/year (Grass-Clover), and (3) Cows offered a total mixed ration diet and housed indoors (TMR). Treatment significantly affected milk production; milk yield and milk solids yield were generally highest on the TMR treatment. There was no effect of treatment on rumen pH. However, treatment significantly altered the rumen volatile fatty acid (VFA), and ammonia and lactic acid profiles. Clover inclusion in the sward led to higher (p < 0.05) total VFA and ammonia concentrations compared to the Grass and TMR treatments. The increased rumen ammonia concentration was associated with a significantly greater milk urea nitrogen (MUN) content in the milk from cows fed on Grass-Clover, indicating a greater excess of dietary protein in that treatment. It was concluded that a clover-based dairy cow feeding system could significantly alter rumen function, milk composition and milk yield. Full article

(This article belongs to the Special Issue Microbiology and Physiology in Ruminant Nutrition)

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16 pages, 721 KiB

Open AccessArticle

Salivary Cortisol Reaction Norms in Zoo-Housed Great Apes: Diurnal Slopes and Intercepts as Indicators of Stress Response Quality

by Verena Behringer, Jeroen M. G. Stevens and Ruth Sonnweber

Animals 2022, 12(4), 522; https://doi.org/10.3390/ani12040522 - 21 Feb 2022

Cited by 4 | Viewed by 2811

Abstract

Monitoring changes in cortisol levels is a widespread tool for measuring individuals’ stress responses. However, an acute increase in cortisol levels does not necessarily denote an individual in distress, as increases in cortisol can be elicited by all factors that signal the need [...] Read more.

Monitoring changes in cortisol levels is a widespread tool for measuring individuals’ stress responses. However, an acute increase in cortisol levels does not necessarily denote an individual in distress, as increases in cortisol can be elicited by all factors that signal the need to mobilize energy. Nor are low levels of cortisol indicative for a relaxed, healthy individual. Therefore, a more fine-grained description of cortisol patterns is warranted in order to distinguish between cortisol fluctuations associated with different stress response qualities. In most species, cortisol shows a distinct diurnal pattern. Using a reaction norm approach, cortisol levels across the day can be described by the two regression coefficients: the intercept and the slope of the curve. We measured immunoreactive salivary cortisol in three zoo-housed ape species under three conditions (routine days, enrichment days, and after the move to a new house). We examined salivary cortisol intercepts (SCI) and salivary cortisol slopes (SCS) of the diurnal curves. SCI and SCS were independent from each other. SCI was highest on enrichment days and lowest on routine days. SCS was steep on routine days and blunted after the move. Only SCI was species-specific. Our study provides evidence that combining SCI and SCS measures allows us to differentiate between types of stress responses, thereby constituting a useful tool for welfare assessment. Full article

(This article belongs to the Special Issue Non-invasive Methods of Stress Monitoring in Animals under Human Care)

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9 pages, 561 KiB

Open AccessArticle

The Concentration of ProAKAP4 and Other Indicators of Cryopotential of Spermatozoa Cryopreserved in Extender with Holothuroidea Extract Addition

by Alicja Kowalczyk, Elżbieta Gałęska and Anna Bubel

Animals 2022, 12(4), 521; https://doi.org/10.3390/ani12040521 - 20 Feb 2022

Cited by 7 | Viewed by 1887

Abstract

The aim of this study was to determine the concentration of proAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with Holothuroidea extract addition. Nine Holstein Friesian bulls, 3.5 years old, of known health status, were used for the study. The [...] Read more.

The aim of this study was to determine the concentration of proAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with Holothuroidea extract addition. Nine Holstein Friesian bulls, 3.5 years old, of known health status, were used for the study. The animals were kept and fed equally. Semen was collected once a week using an artificial vagina. The commercially available Holothuroidea extract was used as a supplement to the commercial extender (0, 2, 4 and 6 µL/mL) before the freezing/thawing process. The viability, motility, motion parameters, and acrosome integrity of the sperm were analyzed with (test) or without (control) extract samples. Furthermore, the concentration of the proAKAP4 biomarker in frozen sperm was assessed. It was shown that the addition of 4 and 6 µL of the extract may have a positive effect on the quality parameters of the sperm after thawing. The results indicate that extender supplementation with the above extract modulates (increases) the concentration of proAKAP4 in sperm at all tested levels. Additionally, this indicator has become helpful in identifying sperm of poor biological quality. Moreover, it has been proven that the proAKAP4 biomarker can be successfully used to evaluate the effectiveness of the use of various extenders for semen cryopreservation. Full article

(This article belongs to the Special Issue Further Approaches on Sperm Cryopreservation)

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14 pages, 2600 KiB

Open AccessArticle

Estimation of the Maternal Investment of Sea Turtles by Automatic Identification of Nesting Behavior and Number of Eggs Laid from a Tri-Axial Accelerometer

by Lorène Jeantet, Vadym Hadetskyi, Vincent Vigon, François Korysko, Nicolas Paranthoen and Damien Chevallier

Animals 2022, 12(4), 520; https://doi.org/10.3390/ani12040520 - 20 Feb 2022

Cited by 2 | Viewed by 2945

Abstract

Monitoring reproductive outputs of sea turtles is difficult, as it requires a large number of observers patrolling extended beaches every night throughout the breeding season with the risk of missing nesting individuals. We introduce the first automatic method to remotely record the reproductive [...] Read more.

Monitoring reproductive outputs of sea turtles is difficult, as it requires a large number of observers patrolling extended beaches every night throughout the breeding season with the risk of missing nesting individuals. We introduce the first automatic method to remotely record the reproductive outputs of green turtles (Chelonia mydas) using accelerometers. First, we trained a fully convolutional neural network, the V-net, to automatically identify the six behaviors shown during nesting. With an accuracy of 0.95, the V-net succeeded in detecting the Egg laying process with a precision of 0.97. Then, we estimated the number of laid eggs from the predicted Egg laying sequence and obtained the outputs with a mean relative error of 7% compared to the observed numbers in the field. Based on deployment of non-invasive and miniature loggers, the proposed method should help researchers monitor nesting sea turtle populations. Furthermore, its use can be coupled with the deployment of accelerometers at sea during the intra-nesting period, from which behaviors can also be estimated. The knowledge of the behavior of sea turtle on land and at sea during the entire reproduction period is essential to improve our knowledge of this threatened species. Full article

(This article belongs to the Special Issue Applications of Accelerometers and Other Bio-Logging Devices in Captive and Wild Animals)

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13 pages, 1245 KiB

Open AccessArticle

Effect of Steroid Hormones, Prostaglandins (E2 and F2α), Oxytocin, and Tumor Necrosis Factor Alpha on Membrane Progesterone (P4) Receptors Gene Expression in Bovine Myometrial Cells

by Magdalena K. Kowalik, Karolina Dobrzyn, Jaroslaw Mlynarczuk and Robert Rekawiecki

Animals 2022, 12(4), 519; https://doi.org/10.3390/ani12040519 - 19 Feb 2022

Cited by 5 | Viewed by 2189

Abstract

Myometrium tissue shows the expression of non-genomic membrane progesterone (P4) receptors, such as progesterone receptor membrane components (PGRMC) 1 and 2 and membrane progestin receptors (mPR) alpha (mPRα), beta (mPRβ), and gamma (mPRγ). Their variable expression in the bovine uterus during the estrous [...] Read more.

Myometrium tissue shows the expression of non-genomic membrane progesterone (P4) receptors, such as progesterone receptor membrane components (PGRMC) 1 and 2 and membrane progestin receptors (mPR) alpha (mPRα), beta (mPRβ), and gamma (mPRγ). Their variable expression in the bovine uterus during the estrous cycle and early pregnancy suggests that ovarian steroids and luteotropic and/or luteolytic factors may regulate the expression of these receptors in the myometrium. Therefore, this study aimed to examine the effect of P4, estradiol (E2), P4 with E2, prostaglandins (PG) E2 and F2α, oxytocin (OT), and tumor necrosis factor α (TNFα) on the gene expression of PGRMC1, PGRMC2, serpine-1 mRNA-binding protein (SERBP1), and mPRα, mPRβ, and mPRγ in bovine myometrial cells from days 6 to 10 and 11 to 16 of the estrous cycle. The PGE2 concentration and mRNA expression were determined by EIA and real-time PCR, respectively. The data indicated that P4 and E2 can affect the mRNA expression of all studied receptors and SERPB1. However, PGE2, OT, and TNFα could only modulate the expression of PGRMC1, PGRMC2, and SERPB1, respectively. Steroids/factors changed the expression of PGRMC and mPR genes depending on the dose, the stage of the estrous cycle, and the types of receptors. This suggests that the local hormonal milieu may influence the activity of these receptors and P4 action in myometrial cells during the estrous cycle. Full article

(This article belongs to the Section Animal Reproduction)

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11 pages, 545 KiB

Open AccessArticle

Effect of Myostatin Gene Mutation on Slaughtering Performance and Meat Quality in Marchigiana Bulls

by Simone Ceccobelli, Francesco Perini, Maria Federica Trombetta, Stefano Tavoletti, Emiliano Lasagna and Marina Pasquini

Animals 2022, 12(4), 518; https://doi.org/10.3390/ani12040518 - 19 Feb 2022

Cited by 9 | Viewed by 2859

Abstract

The myostatin gene also called Growth Differentiation Factor 8 gene (GDF8) is one of the most investigated loci that can be responsible for several quantitative and qualitative carcass and meat traits in double-muscled beef cattle. The objective of the study was [...] Read more.

The myostatin gene also called Growth Differentiation Factor 8 gene (GDF8) is one of the most investigated loci that can be responsible for several quantitative and qualitative carcass and meat traits in double-muscled beef cattle. The objective of the study was to bring to light the effect of the myostatin polymorphism on slaughtering performance and meat quality in Marchigiana beef cattle. The experiment was carried out on 78 bulls reared according to the “cow-calf” extensive managing system. At the end of the fattening period, in vivo and carcass data were recorded. From each carcass, a steak of Longissimus thoracis was taken and used to determine the meat’s analytical composition and colorimetric properties. Finally, from each steak a sample of Longissimus thoracis was collected, then used for DNA extraction and genotyping at the myostatin locus. The heterozygous bulls showed slight superiority in the carcass data (e.g., hot carcass weight: 426.09 kg—heterozygotes vs. 405.32 kg—normal) and meat quality parameters, although not always with statistical significance. Only fat and ashes content were significantly affected by the myostatin genotype (heterozygotes: 2.01%, 1.26%; normal: 3.04%, 1.15%). The greater muscularity of heterozygous animals compared to normal ones could be a starting point to improving productive efficiency in Marchigiana beef cattle. Full article

(This article belongs to the Special Issue The Myostatin Gene: Future Challenges in Animal Science)

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18 pages, 2606 KiB

Open AccessReview

The Shape of the Nasal Cavity and Adaptations to Sniffing in the Dog (Canis familiaris) Compared to Other Domesticated Mammals: A Review Article

by Anna Buzek, Katarzyna Serwańska-Leja, Anita Zaworska-Zakrzewska and Małgorzata Kasprowicz-Potocka

Animals 2022, 12(4), 517; https://doi.org/10.3390/ani12040517 - 19 Feb 2022

Cited by 9 | Viewed by 29146

Abstract

Dogs are a good starting point for the description and anatomical analysis of turbinates of the nose. This work aimed at summing up the state of knowledge on the shape of the nasal cavity and airflow in these domestic animals and dealt with [...] Read more.

Dogs are a good starting point for the description and anatomical analysis of turbinates of the nose. This work aimed at summing up the state of knowledge on the shape of the nasal cavity and airflow in these domestic animals and dealt with the brachycephalic syndrome (BOAS) and anatomical changes in the initial airway area in dogs with a short and widened skull. As a result of artificial selection and breeding concepts, the dog population grew very quickly. Modern dog breeds are characterized by a great variety of their anatomical shape. Craniological changes also had a significant impact on the structure and physiology of the respiratory system in mammals. The shape of the nasal cavity is particularly distinctive in dogs. Numerous studies have established that dogs and their olfactory ability are of great importance in searching for lost people, detecting explosives or drugs as well as signaling disease in the human body. The manuscript describes the structure of the initial part of the respiratory system, including the nasal turbinates, and compares representatives of various animal species. It provides information on the anatomy of brachycephalic dogs and BOAS. The studies suggest that further characterization and studies of nasal turbinates and their hypertrophy are important. Full article

(This article belongs to the Special Issue From the Nose to the Brain: Animals' Olfactory and Vomeronasal Systems)

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9 pages, 484 KiB

Open AccessReview

Characteristics and Applications of Canine In Vitro Models of Bladder Cancer in Veterinary Medicine: An Up-to-Date Mini Review

by Łukasz Nowak, Wojciech Krajewski, Bartosz Małkiewicz, Tomasz Szydełko and Aleksandra Pawlak

Animals 2022, 12(4), 516; https://doi.org/10.3390/ani12040516 - 19 Feb 2022

Cited by 1 | Viewed by 2277

Abstract

Bladder cancer (BC) constitutes approximately 2% of all spontaneously occurring cancers in dogs. It is characterized by a devastating clinical course in most cases, which emphasizes a constant need for the development of novel methods of disease characterization and treatment. Over the past [...] Read more.

Bladder cancer (BC) constitutes approximately 2% of all spontaneously occurring cancers in dogs. It is characterized by a devastating clinical course in most cases, which emphasizes a constant need for the development of novel methods of disease characterization and treatment. Over the past years, advances in cell engineering have resulted in the development of various canine in vitro models of BC, emerging as complements for in vivo research. In this article, we aimed to review the available data on existing in vitro models of canine BC, focusing primarily on their characteristics, applications in veterinary medicine, as well as advantages and disadvantages. The most commonly used in vitro models of canine BC comprise immortalized cell lines grown as adherent monolayers. They provide an unlimited supply of research material, however, they do not faithfully reflect the conditions prevailing in vivo, since the spatial cellular interactions are lost. The importance of the three-dimensional (3D) features of solid tumors in relation to carcinogenesis or drug response process has resulted in the development of the first canine 3D models of BC available for in vitro research. So far, results obtained with in vitro and in vivo research should be interpreted together. With the constantly growing complexity of in vitro models of BC cancer, animal-based research might be reduced in the future. Full article

(This article belongs to the Special Issue Tumours and Leukocyte Research in Livestock and Companion Animals)

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16 pages, 475 KiB

Open AccessArticle

Characterization of Fat Quality in Cow Milk from Alpine Farms as Influenced by Seasonal Variations of Diets

by Annalaura Lopez, Federica Bellagamba, Giovanni Savoini, Vittorio Maria Moretti and Donata Cattaneo

Animals 2022, 12(4), 515; https://doi.org/10.3390/ani12040515 - 19 Feb 2022

Cited by 3 | Viewed by 2213

Abstract

The production systems linked to mountain animal husbandry have had an environmental, social and cultural role in recent years. Zootechnical systems based on feeding strategies, such as pasture grazing and grass-fed strategies, contribute to a significant increase in the relative amounts of favorable [...] Read more.

The production systems linked to mountain animal husbandry have had an environmental, social and cultural role in recent years. Zootechnical systems based on feeding strategies, such as pasture grazing and grass-fed strategies, contribute to a significant increase in the relative amounts of favorable fatty acids (FAs) in animal products, indicating their ability to improve the long-term health of consumers. In this study, we compared different feeding strategies in two small mountain farms in the Piedmont Alpine region, Italy. Particularly, during the summer season, the two farms were distinguished by the exclusive employment of Alpine pasture (farm A), assumed as the best way to improve the quality of the FA profile in milk vs. the supply of daily fresh cut mountain grass plus a reduced implementation with hay and concentrates directly in the barn (farm B). The milk fatty acid profile was analyzed using gas chromatography. The results showed the high quality of alpine milk collected in the two farms. Even with some differences, particularly evidenced when comparing the summer diets, the milk FA profiles in farm A and farm B were favorable from a nutritional point of view in both seasons. Milk samples obtained using the exclusive employment of alpine grazing during summer were represented by an FA profile of higher quality (lower saturated FAs, higher branched FAs and monounsaturated FA, favorable n6/n3 ratio). However, milk obtained using the integrated strategy (fresh grass plus concentrates in the barn farm B) resulted in a more homogenous composition during the summer season, with a higher concentration of polyunsaturated FAs. These outcomes suggested that the integrated strategy, even if related to a lower ability in improving milk FA profile, could represent a valid and cost-effective alternative for mountain farmers to obtain an overall superior quality of milk, which was not strictly linked to the grazing practice. The multivariate analysis showed that information contained in the milk FA profile may provide a valuable tool that can distinguish mountain-grass-based diet. Full article

(This article belongs to the Special Issue Frontiers in Livestock Production, Health and Environmental Effects)

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12 pages, 17995 KiB

Open AccessArticle

The Effect of Environmental Enrichment on Laboratory Rare Minnows (Gobiocypris rarus): Growth, Physiology, and Behavior

by Chunsen Xu, Miaomiao Hou, Liangxia Su, Ning Qiu, Fandong Yu, Xinhua Zou, Chunling Wang, Jianwei Wang and Yongfeng He

Animals 2022, 12(4), 514; https://doi.org/10.3390/ani12040514 - 18 Feb 2022

Cited by 4 | Viewed by 1857

Abstract

Environmental enrichment is a method to increase environmental heterogeneity, which may reduce stress and improve animal welfare. Previous studies have shown that environmental enrichment can increase the growth rate, decrease aggressive and anxiety-like behaviors, improve learning ability and agility, and reduce cortisol levels [...] Read more.

Environmental enrichment is a method to increase environmental heterogeneity, which may reduce stress and improve animal welfare. Previous studies have shown that environmental enrichment can increase the growth rate, decrease aggressive and anxiety-like behaviors, improve learning ability and agility, and reduce cortisol levels in animals. These effects usually differ between species. Unfortunately, habitat enrichment on laboratory fish is poorly studied and seldom adopted in care guidance. Rare minnows (Gobiocypris rarus) have been cultured as a native laboratory fish in China in barren banks without environmental enrichment since 1990; they have been widely used in studies on ecotoxicology, environmental science, and other topics. The purpose of this study was to investigate the effect of environment enrichment on the growth, physiological status, and anxiety-like behavior of laboratory rare minnows. We observed and analyzed SGR, cortisol levels, DA, DOPAC, 5-HT and 5-HIAA, and anxiety-like behavior indexes after one month of treatment in barren (control) and enrichment tanks. We found that there were no significant differences in SGR, anxiety-like behavior, DA, DOPAC, or 5-HIAA levels between the two treatments. However, higher cortisol and 5-HT levels were observed in the enrichment tanks. This study suggests that rare minnows might be influenced by their living environment, and future related studies should consider their environmental enrichment. Full article

(This article belongs to the Special Issue Animal Welfare Assessment: Novel Approaches and Technologies)

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14 pages, 619 KiB

Open AccessArticle

Lower Reproductive Rate and Lamb Survival Contribute to Lower Lamb Marking Rate in Maiden Ewes Compared to Multiparous Ewes

by Dayna Hutchison, Bronwyn E. Clarke, Serina Hancock, Andrew N. Thompson, Elise Bowen and Caroline Jacobson

Animals 2022, 12(4), 513; https://doi.org/10.3390/ani12040513 - 18 Feb 2022

Cited by 7 | Viewed by 1952

Abstract

Suboptimal reproductive performance of maiden (primiparous) ewes remains a source of inefficiency for the Australian sheep industry. However, the extent and causes of the poorer reproductive performance of maiden ewes on Australian sheep farms are not well understood. Here, we show the reproductive [...] Read more.

Suboptimal reproductive performance of maiden (primiparous) ewes remains a source of inefficiency for the Australian sheep industry. However, the extent and causes of the poorer reproductive performance of maiden ewes on Australian sheep farms are not well understood. Here, we show the reproductive performance of maiden ewes relative to their multiparous counterparts on the same farms across Australia using a cohort survey. The difference in marking rate for non-Merino maiden ewe lambs compared to multiparous ewes was 58% (74 vs. 132%; p < 0.001), and this was attributable to a 50% difference in reproductive rate (109 vs. 159%; p < 0.001) and 16% difference in lamb survival to marking (67 vs. 83%; p < 0.001). The difference in marking rate for maiden Merino two-tooth ewes lambing at approximately 2 years-of-age compared to mature multiparous ewes was 22% (80 vs. 102%; p < 0.001) and this was attributable to a 24% difference in reproductive rate (108 vs. 132%; p < 0.001) and 3% difference for lamb survival (75 vs. 78%; p < 0.05). Positive correlations for reproduction traits (reproductive rate, lamb survival and marking rate) between maidens and multiparous ewes were observed for maiden Merino two-tooth ewes (p < 0.001), but these correlations were weak or non-existent for non-Merino ewe lambs. Strategies to improve both reproductive rate and lamb survival can address the poorer and more variable reproductive performance of maiden ewes. Full article

(This article belongs to the Special Issue Hogget Production and Longevity)

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17 pages, 1175 KiB

Open AccessArticle

Dairy Cow Behavior Is Affected by Period, Time of Day and Housing

by Lisette M. C. Leliveld, Elisabetta Riva, Gabriele Mattachini, Alberto Finzi, Daniela Lovarelli and Giorgio Provolo

Animals 2022, 12(4), 512; https://doi.org/10.3390/ani12040512 - 18 Feb 2022

Cited by 8 | Viewed by 2708

Abstract

Dairy cow behavior is affected by external and endogenous factors, including time of year, barn microclimate, time of day and housing. However, little is known about the combined effects of these factors. Data were collected on eight farms in Northern Italy during summer, [...] Read more.

Dairy cow behavior is affected by external and endogenous factors, including time of year, barn microclimate, time of day and housing. However, little is known about the combined effects of these factors. Data were collected on eight farms in Northern Italy during summer, winter and a temperate season. The temperature-humidity index (THI) was recorded using environmental sensors, whereas cow behavior was monitored using leg accelerometers and cameras. Period, time of day and their interaction all significantly affected lying, standing and feeding behavior. However, although THI had a significant negative effect on lying and a positive effect on standing during daytime (all p < 0.001), during nighttime, it only had a significant negative effect on lying duration and mean lying bout duration (p < 0.001 for both). There was also significant variation between farms in all behavioral parameters, as well as interactions with period and time of day. For instance, farm differences in lying duration were more pronounced during daytime than during nighttime. These findings show how housing can interact with other factors, such as period of the year and time of day, and illustrate the influence of barn structure and farm management on cow behavior and, consequently, their welfare. Full article

(This article belongs to the Special Issue Housing Systems in Dairy Production)

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12 pages, 696 KiB

Open AccessArticle

Antibacterial Potential of Caesalpinia coriaria (Jacq) Willd Fruit against Aeromonas spp. of Aquaculture Importance

by Lenin Rangel-López, Nallely Rivero-Perez, Benjamín Valladares-Carranza, Agustín Olmedo-Juárez, Lucía Delgadillo-Ruiz, Vicente Vega-Sánchez, Sawako Hori-Oshima, Mohamed A. Nassan, Gaber El-Saber Batiha and Adrian Zaragoza-Bastida

Animals 2022, 12(4), 511; https://doi.org/10.3390/ani12040511 - 18 Feb 2022

Cited by 8 | Viewed by 2250

Abstract

Aquaculture is an important source of food and livelihood for hundreds of millions of people around the world, however, aquaculture systems are affected by different factors, among them the appearance of resistant or multiresistant bacteria to antimicrobials. The secondary metabolites of plants have [...] Read more.

Aquaculture is an important source of food and livelihood for hundreds of millions of people around the world, however, aquaculture systems are affected by different factors, among them the appearance of resistant or multiresistant bacteria to antimicrobials. The secondary metabolites of plants have been proposed as alternatives for the treatment of these bacteria. The aim of the present study was to determine the antibacterial activity of Caesalpinia coriaria fruit hydroalcoholic extract and gallic acid over Aeromonas hydrophila, Aeromonas veronii, and Aeromonas dhakensis to identify new molecules for the treatment of diseases caused by Aeromonas spp. The C. coriaria fruit hydroalcoholic extract (HECc) was obtained by hydroalcoholic maceration and subjected to bipartition with ethyl acetate and water to obtain an aqueous fraction (Ac-FrCc) and an organic fraction (Ac-FrEtCc); gallic acid was purchased commercially. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), MBC/MIC ratio, and cytotoxicity of HECc, its fractions, and gallic acid were determined. The results indicate that HECc fractions (Ac-FrCc and Ac-FrEtCc) and gallic acid have bactericidal activity against A. hydrophila and A. dhakensis, but only gallic acid showed bactericidal activity against A. veronii. The HECc and Ac-FrCc showed no toxicity, Ac-FrEtCc showed low toxicity, and gallic acid showed medium toxicity. The HECc, Ac-FrCc, and Ac-FrEtCc may be alternatives for the treatment of diseases caused by the genus Aeromonas, however, in vivo assays are necessary to corroborate these results. Full article

(This article belongs to the Special Issue Advances on Alternatives to Conventional Antimicrobials: Implications on Livestock, Aquaculture, and Poultry Production)

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12 pages, 295 KiB

Open AccessArticle

Milk Supplemented with Organic Iron Improves Performance, Blood Hematology, Iron Metabolism Parameters, Biochemical and Immunological Parameters in Suckling Dalagh Lambs

by Mohammad Asadi, Abdolhakim Toghdory, Maryam Hatami and Jalil Ghassemi Nejad

Animals 2022, 12(4), 510; https://doi.org/10.3390/ani12040510 - 18 Feb 2022

Cited by 7 | Viewed by 2875

Abstract

This study was conducted to investigate the effect of milk supplemented with organic iron on performance, blood hematology, iron metabolism parameters, biochemical and immunological parameters in suckling lambs. Thirty-six newborn Dalagh lambs were randomly divided into three groups with 12 replications. The control [...] Read more.

This study was conducted to investigate the effect of milk supplemented with organic iron on performance, blood hematology, iron metabolism parameters, biochemical and immunological parameters in suckling lambs. Thirty-six newborn Dalagh lambs were randomly divided into three groups with 12 replications. The control group was fed with milk without organic iron. The other two groups were fed milk supplemented with 25 and 50 mg/d organic iron, respectively. During the experiment, increased daily weight gain and total body weight were observed in the iron-supplemented groups. An increase in the levels of red blood cell, hemoglobin, hematocrit, mean corpuscular hemoglobin, and mean corpuscular concentration in iron supplemented groups was indicated. Consumption of organic iron caused a significant decrease in plasma copper concentration. Total antioxidant status level was lower, but levels of glutathione peroxidase, superoxide dismutase, and catalase were higher in iron supplemented groups. In organic iron supplemented groups, insulin and thyroid hormones levels were significantly increased, and glucose level was significantly decreased. In organic iron supplemented groups, alkaline phosphatase level significantly increased, and aminotransferase level significantly decreased. Overall, the use of organic iron in the milk improved the performance and health in suckling lambs, and since a lower level of supplementation is naturally preferable, supplementation of milk with 25 mg/d organic iron is recommended. Full article

(This article belongs to the Section Small Ruminants)

13 pages, 1722 KiB

Open AccessArticle

Immediate Postoperative Analgesia of Nalbuphine-Ketamine Combination Compared with Ketamine Alone in Xylazine-Sedated Goats Undergoing Left Flank Laparotomy

by Mahmoud M. Abouelfetouh, Eman Salah, Lingling Liu, Ahmed H. Khalil, Qiulin Zhang, Mingxing Ding and Yi Ding

Animals 2022, 12(4), 509; https://doi.org/10.3390/ani12040509 - 18 Feb 2022

Cited by 1 | Viewed by 2027

Abstract

Goats have been used as animal models in research, and the need for achieving safer anesthesia for research or surgical intervention is gaining much attention. The objective of this study was to evaluate intraoperative effects and the immediate postoperative analgesia of nalbuphine–ketamine regimen [...] Read more.

Goats have been used as animal models in research, and the need for achieving safer anesthesia for research or surgical intervention is gaining much attention. The objective of this study was to evaluate intraoperative effects and the immediate postoperative analgesia of nalbuphine–ketamine regimen in goats. Twenty clinically healthy adult female crossbred goats weighing 14 ± 2 kg were allocated randomly into each of two equally sized groups. All animals were sedated with intramuscular (IM) xylazine (0.07 mg/kg), then anesthesia was intravenously (IV) induced with ketamine alone (10 mg/kg) (XK group), or a combination of nalbuphine (0.5 mg/kg) and ketamine (5 mg/kg) (XNK group). Following induction, left flank laparotomy was performed and then sutured. The quality of anesthesia and immediate postoperative analgesia was evaluated. Immediate postoperative analgesia was assessed up to 5 h after standing, using a modified Unesp–Botucatu acute composite pain scale (USAPS). Serum cortisol, glucose, insulin, and C-reactive protein (CRP) were measured at ½, 1, 2, 4, 6, 12, and 24 h, postoperatively (PO). The USAPS pain scores were significantly lower in the XNK compared to the XK group (p < 0.05). The XNK group exhibited a statistically significant difference in the level of serum cortisol at ½ and 1 h PO (p = 0.018 and 0.045, respectively) compared to the XK group. At 2, 4, 6 h PO, CRP significantly decreased (p = 0.023, 0.040 and 0.005, respectively) in the XNK compared to the XK group. Nalbuphine–ketamine produced an acceptable induction of anesthesia and recovery compared to ketamine. Recovery with nalbuphine–ketamine was faster and better quality. The USAPS pain scores were lower in nalbuphine–ketamine, indicating that this novel combination produces better postoperative pain control than ketamine alone. Full article

(This article belongs to the Section Small Ruminants)

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9 pages, 3993 KiB

Open AccessCase Report

Pulmonary and Systemic Skeletal Muscle Embolism in a Beaked Whale with a Massive Trauma of Unknown Aetiology

by Eva Sierra, Tania Ramírez, Yara Bernaldo de Quirós, Marina Arregui, Blanca Mompeó, Miguel A. Rivero and Antonio Fernández

Animals 2022, 12(4), 508; https://doi.org/10.3390/ani12040508 - 17 Feb 2022

Cited by 2 | Viewed by 1992

Abstract

An adult female Sowerby’s beaked whale was found floating dead in Hermigua (La Gomera, Canary Islands, Spain) on 7 December 2016. Severe traumas of unknown aetiology were attributed, and the gross and microscopic findings are consistent with catastrophic trauma as a cause of [...] Read more.

An adult female Sowerby’s beaked whale was found floating dead in Hermigua (La Gomera, Canary Islands, Spain) on 7 December 2016. Severe traumas of unknown aetiology were attributed, and the gross and microscopic findings are consistent with catastrophic trauma as a cause of death. Rib fractures affected the intercostals, transverse thoracis skeletal muscles, and thoracic rete mirabile. Degenerated muscle fibres were extruded to flow into vascular and lymphatic vessels travelling to several anatomic locations into the thoracic cavity, including the lungs, where they occluded the small lumen of pulmonary microvasculature. A pulmonary and systemic skeletal muscle embolism was diagnosed, constituting the first description of this kind of embolism in an animal. The only previous description has been reported in a woman after peritoneal dialysis. Skeletal pulmonary embolism should be considered a valuable diagnostic for different types of trauma in vivo in wild animals. This is especially valuable when working with decomposed carcasses, as in those cases, it is not always feasible to assess other traumatic evidence. Full article

(This article belongs to the Special Issue Pathology of Marine Mammals)

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11 pages, 2149 KiB

Open AccessArticle

Detection and Genomic Characterization of Canine Circovirus in Iran

by Farzad Beikpour, Linda Amarachi Ndiana, Alireza Sazmand, Paolo Capozza, Farzad Nemati, Francesco Pellegrini, Salman Zafari, Seyed Masoud Zolhavarieh, Roberta Cardone, Reza Faraji, Gianvito Lanave, Vito Martella and Nicola Decaro

Animals 2022, 12(4), 507; https://doi.org/10.3390/ani12040507 - 17 Feb 2022

Cited by 11 | Viewed by 2242

Abstract

Canine circovirus (CaCV) is a single-stranded DNA virus that globally circulates in dogs and wild carnivores. Although the pathogenic potential of the virus has not been fully understood yet, CaCV has been suggested to exacerbate the clinical course of other canine viral infections [...] Read more.

Canine circovirus (CaCV) is a single-stranded DNA virus that globally circulates in dogs and wild carnivores. Although the pathogenic potential of the virus has not been fully understood yet, CaCV has been suggested to exacerbate the clinical course of other canine viral infections but also to circulate in dogs without clinical signs. In this study, we carried out real-time PCR assays to detect enteric pathogens from 156 canine rectal swabs collected from dogs without enteritis in 3 different regions in Iran. A total of 14 samples tested positive for CaCV and full-length genome sequences were obtained from 6 of the detected strains. Sequence and phylogenetic analyses showed that, despite the distance between the different sample collection sites, all Iranian CaCV strains were closely related and formed a separate clade from extant CaCVs. The present study shows that CaCV is circulating in non-diarrheic dogs in Iran, thus highlighting the need for further epidemiological investigations in Iranian domestic and wild carnivores. Full article

(This article belongs to the Section Companion Animals)

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19 pages, 3507 KiB

Open AccessArticle

A Field-Deployable Insulated Isothermal PCR (iiPCR) for the Global Surveillance of Toxoplasma gondii Infection in Cetaceans

by Meng-Jung Hsieh and Wei-Cheng Yang

Animals 2022, 12(4), 506; https://doi.org/10.3390/ani12040506 - 17 Feb 2022

Cited by 5 | Viewed by 3435

Abstract

Toxoplasmosis is a zoonotic disease with veterinary and public health importance worldwide. Toxoplasma gondii infection in cetaceans is an indicator of land-to-sea oocyst pollution. However, there is a critical knowledge gap within the distribution of the T. gondii infection in cetaceans. To facilitate [...] Read more.

Toxoplasmosis is a zoonotic disease with veterinary and public health importance worldwide. Toxoplasma gondii infection in cetaceans is an indicator of land-to-sea oocyst pollution. However, there is a critical knowledge gap within the distribution of the T. gondii infection in cetaceans. To facilitate the global surveillance of this important zoonotic pathogen, we developed a field-deployable duplex insulated isothermal PCR (iiPCR) with automated magnetic bead-based DNA extraction for the on-site detection of T. gondii in stranded cetaceans. It targets the B1 gene of T. gondii combined with β2-microglobulin (B2M) gene of cetaceans as an internal control. Compared with the conventional qPCR assay, B1/B2M duplex iiPCR assay showed comparable sensitivity (21~86 bradyzoites in 25 mg of tissue) to detect spike-in standard of T. gondii DNA in cerebrum, cerebellum, skeletal muscle and myocardium tissues. Moreover, the overall agreement between the duplex iiPCR and qPCR was in almost perfect agreement (92%; 95% CI: 0.78–0.90; κ = 0.84) in detecting a synthetic spike-in standards. The B1/B2M iiPCR assay coupled with a field-deployable system provides a prompt (~1.5 h), feasible, highly sensitive and specific on-site diagnostic tool for T. gondii in stranded cetaceans. This platform provides one approach to evaluating aquatic ecosystem health and developing early warnings about negative impacts on humans and marine animals. Full article

(This article belongs to the Section Aquatic Animals)

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15 pages, 6717 KiB

Open AccessArticle

MiR-24-3p Conservatively Regulates Muscle Cell Proliferation and Apoptosis by Targeting Common Gene CAMK2B in Rat and Cattle

by Ge Yang, Mingli Wu, Xinqi Liu, Fuwen Wang, Mei Li, Xiaoya An, Fuxia Bai, Chuzhao Lei and Ruihua Dang

Animals 2022, 12(4), 505; https://doi.org/10.3390/ani12040505 - 17 Feb 2022

Cited by 4 | Viewed by 2390

Abstract

Skeletal muscle plays an important role in the growth and development of meat animals. MicroRNAs (miRNAs) can participate in the regulation of muscle development-related functions; however, there have been few reports on whether there are related miRNAs that conservatively regulate muscle development among [...] Read more.

Skeletal muscle plays an important role in the growth and development of meat animals. MicroRNAs (miRNAs) can participate in the regulation of muscle development-related functions; however, there have been few reports on whether there are related miRNAs that conservatively regulate muscle development among different species. In this study, the miRNA transcriptome sequencing data of the muscle tissue of cattle, rat, goat, and pig showed that miR-24-3p may conservatively regulate muscle development in these species. Furthermore, mmu-miR-24-3p can positively regulate C2C12 cell proliferation and apoptosis by regulating key proliferation and apoptosis genes in muscle development, which was verified by CCK-8 and RT-qPCR. Bta-miR-24-3p can also positively regulate the proliferation and apoptosis of bovine muscle primary cells by regulating key proliferation and apoptosis genes in the process of muscle development, as verified by CCK-8 and RT-qPCR. The target genes of miR-24-3p in cattle, rat, goat, and pig, which include a large proportion of target genes shared among the four species, are enriched in multiple cell functions and signal pathways that are closely related to muscle development, as revealed by GO and KEGG enrichment analysis. A double luciferase test showed that the shared target genes WNT4, CAMK2B, and TCF7 were targeted by mmu-miR-24-3p in rat and bta-miR-24-3p in cattle. These three shared target genes WNT4, CAMK2B, and TCF7 are involved in the Wnt signaling pathway, which showed that miR-24-3p plays an important role in rat and cattle. The shared target gene (CAMK2B) in rat and cattle increased significantly after the inhibition of miR-24-3p by RT-qPCR. The findings of this study contribute to a better understanding of the role of miR-24-3p in the regulation of muscle development. Full article

(This article belongs to the Special Issue RNA-Sequencing Technique in Animals Research)

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12 pages, 4598 KiB

Open AccessArticle

The High Expression of Legumain in Canine Neoplasms: A Retrospective Analysis of 100 Cases

by Chiao-Hsu Ke, Ka-Mei Sio, Shang-Lin Wang, Ying Kuo, Wei-Hsiang Huang and Chen-Si Lin

Animals 2022, 12(4), 504; https://doi.org/10.3390/ani12040504 - 17 Feb 2022

Cited by 3 | Viewed by 1932

Abstract

Legumain, a novel asparaginyl endopeptidase, has been observed to be overexpressed in several types of human solid tumors. Elevated levels of legumain are found in human cancers, and this oncoprotein may facilitate tumor invasion and metastasis when overexpressed. These findings suggest that legumain [...] Read more.

Legumain, a novel asparaginyl endopeptidase, has been observed to be overexpressed in several types of human solid tumors. Elevated levels of legumain are found in human cancers, and this oncoprotein may facilitate tumor invasion and metastasis when overexpressed. These findings suggest that legumain plays a malignant role in cancer biology. However, currently, no publications have identified the role of legumain in the development of canine cancers. The present study first compared the expression patterns of legumain in paraffin-embedded canine tumor tissues, with those of normal tissues, by immunohistochemistry. A total of 100 canine tumor samples, including mast cell tumors, soft tissue sarcoma, hemangiosarcoma, lymphoma, mammary gland carcinoma, hepatoid gland tumor, squamous cell carcinoma, trichoblastoma, and melanoma were evaluated. Compared with the normal tissues, all tumor samples displayed high intensities of legumain expression. Mesenchymal-type tumors displayed immunoreactivity for legumain, with an average expression of 40.07% ± 1.70%, which was significantly lower than those of epithelial tumors and other types of tumors, which had median expressions of 49.12% ± 1.75% and 47.35% ± 2.71%, respectively (p < 0.05). These findings indicate that legumain has a high potential to be a candidate for distinguishing tumors from normal tissues. Although further studies on a larger number of cases are necessary to clarify the clinical application of legumain, the overexpression patterns of legumain in canine tumor tissues are reported, for the first time, in this study. Full article

(This article belongs to the Special Issue Canine and Feline Tumors: Research Advances and Diagnostic Innovations)

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11 pages, 721 KiB

Open AccessArticle

Distribution of CRISPR in Escherichia coli Isolated from Bulk Tank Milk and Its Potential Relationship with Virulence

by Hyo-Jung Kang and Young-Ju Lee

Animals 2022, 12(4), 503; https://doi.org/10.3390/ani12040503 - 17 Feb 2022

Cited by 2 | Viewed by 1840

Abstract

Escherichia coli is one of the most common causes of mastitis on dairy farms around the world, but its clinical severity is determined by a combination of virulence factors. Recently, clustered regularly interspaced short palindromic repeat (CRISPR) arrays have been reported as a [...] Read more.

Escherichia coli is one of the most common causes of mastitis on dairy farms around the world, but its clinical severity is determined by a combination of virulence factors. Recently, clustered regularly interspaced short palindromic repeat (CRISPR) arrays have been reported as a novel typing method because of their usefulness in discriminating pathogenic bacterial isolates. Therefore, this study aimed to investigate the virulence potential of E. coli isolated from bulk tank milk, not from mastitis, and to analyze its pathogenic characterization using the CRISPR typing method. In total, 164 (89.6%) out of 183 E. coli isolated from the bulk tank milk of 290 farms carried one or more of eighteen virulence genes. The most prevalent virulence gene was fimH (80.9%), followed by iss (38.3%), traT (26.8%), ompT (25.7%), afa/draBC (24.0%), and univcnf (21.9%). Moreover, the phylogenetic group with the highest prevalence was B1 (64.0%), followed by A (20.1%), D (8.5%), and C (7.3%) (p < 0.05). Among the four CRISPR loci, only two, CRISPR 1 and CRISPR 2, were found. Interestingly, the distribution of CRISPR 1 was significantly higher in groups A and B1 compared to that of CRISPR 2 (p < 0.05), but there were no significant differences in groups C and D. The prevalence of CRISPR 1 by virulence gene ranged from 91.8% to 100%, whereas that of CRISPR 2 ranged from 57.5% to 93.9%. The distribution of CRISPR 1 was significantly higher in fimH, ompT, afa/draBC, and univcnf genes than that of CRISPR 2 (p < 0.05). The most prevalent E. coli sequence types (EST) among 26 ESTs was EST 22 (45.1%), followed by EST 4 (23.2%), EST 16 (20.1%), EST 25 (19.5%), and EST 24 (18.3%). Interestingly, four genes, fimH, ompT, afa/draBC, and univcnf, had a significantly higher prevalence in both EST 4 and EST 22 (p < 0.05). Among the seven protospacers derived from CRISPR 1, protospacer 163 had the highest prevalence (20.4%), and it only existed in EST 4 and EST 22. This study suggests that the CRISPR sequence-typing approach can help to clarify and trace virulence potential, although the E. coli isolates were from normal bulk tank milk and not from mastitis. Full article

(This article belongs to the Section Cattle)

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Animals, Volume 12, Issue 4 (February-2 2022) – 126 articles

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