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Animals
Volume 12
Issue 24
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Animals, Volume 12, Issue 24 (December-2 2022) – 148 articles

Cover Story (view full-size image): Nutrient requirements of animals are estimated by measuring the nutrients captured in animal products—changes in body composition of growing cattle. Proximate chemical analysis of whole animals is expensive, and thus, other methods such as urea space and carcass specific gravity have been developed; however, these methods only measure one chemical component, requiring estimation of other components through established relationships. Equations relating chemical components were developed over 40 years ago using different cattle genetics and production systems than those used currently. This analysis of published equations indicates that estimation of changes in body weight and chemical composition could be improved by including other factors such as sex and breed type, and that prediction equations may need to be re-evaluated periodically as cattle genetics and production systems change. View this paper
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12 pages, 1439 KiB  
Article
Genome-Wide Identification and Phylogenetic Analysis of TRP Gene Family Members in Saurian
by Lin Zhang, Ning Li, Buddhi Dayananda, Lihu Wang, Huimin Chen and Yunpeng Cao
Animals 2022, 12(24), 3593; https://doi.org/10.3390/ani12243593 - 19 Dec 2022
Cited by 1 | Viewed by 1522
Abstract
The transient receptor potential plays a critical role in the sensory nervous systems of vertebrates in response to various mechanisms and stimuli, such as environmental temperature. We studied the physiological adaptive evolution of the TRP gene in the saurian family and performed a [...] Read more.
The transient receptor potential plays a critical role in the sensory nervous systems of vertebrates in response to various mechanisms and stimuli, such as environmental temperature. We studied the physiological adaptive evolution of the TRP gene in the saurian family and performed a comprehensive analysis to identify the evolution of the thermo-TRPs channels. All 251 putative TRPs were divided into 6 subfamilies, except TRPN, from the 8 saurian genomes. Multiple characteristics of these genes were analyzed. The results showed that the most conserved proteins of TRP box 1 were located in motif 1, and those of TRP box 2 were located in motif 10. The TRPA and TRPV in saurian tend to be one cluster, as a sister cluster with TRPC, and the TRPM is the root of group I. The TRPM, TRPV, and TRPP were clustered into two clades, and TRPP were organized into TRP PKD1-like and PKD2-like. Segmental duplications mainly occurred in the TRPM subfamily, and tandem duplications only occurred in the TRPV subfamily. There were 15 sites to be under positive selection for TRPA1 and TRPV2 genes. In summary, gene structure, chromosomal location, gene duplication, synteny analysis, and selective pressure at the molecular level provided some new evidence for genetic adaptation to the environment. This result provides a basis for identifying and classifying TRP genes and contributes to further elucidating their potential function in thermal sensors. Full article
(This article belongs to the Section Herpetology)
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23 pages, 11601 KiB  
Article
Intra- and Interspecies RNA-Seq Based Variants in the Lactation Process of Ruminants
by Mohammad Farhadian, Seyed Abbas Rafat, Christopher Mayack and Mehdi Bohlouli
Animals 2022, 12(24), 3592; https://doi.org/10.3390/ani12243592 - 19 Dec 2022
Viewed by 1329
Abstract
The RNA-Seq data provides new opportunities for the detection of transcriptome variants’ single nucleotide polymorphisms (SNPs) in various species and tissues. Herein, milk samples from two sheep breeds and two cow breeds were utilized to characterize the genetic variation in the coding regions [...] Read more.
The RNA-Seq data provides new opportunities for the detection of transcriptome variants’ single nucleotide polymorphisms (SNPs) in various species and tissues. Herein, milk samples from two sheep breeds and two cow breeds were utilized to characterize the genetic variation in the coding regions in three stages (before-peak (BP), peak (P), and after-peak (AP)) of the lactation process. In sheep breeds Assaf and Churra, 100,462 and 97,768, 65,996 and 62,161, and 78,656 and 39,245 variants were observed for BP, P, and AP lactation stages, respectively. The number of specific variants was 59,798 and 76,419, 11,483 and 49,210, and 104,033 and 320,817 in cow breeds Jersy and Kashmiri, respectively, for BP, P, and AP stages. Via the transcriptome analysis of variation in regions containing QTL for fat, protein percentages, and milk yield, we detected a number of pathways and genes harboring mutations that could influence milk production attributes. Many SNPs detected here can be regarded as appropriate markers for custom SNP arrays or genotyping platforms to conduct association analyses among commercial populations. The results of this study offer new insights into milk production genetic mechanisms in cow and sheep breeds, which can contribute to designing suitable breeding systems for optimal milk production. Full article
(This article belongs to the Special Issue Effect of Genetic and Nongenetic Factors on Functional and Milk Production Traits in Livestock)
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14 pages, 269 KiB  
Article
Fur, Fin, and Feather: Management of Animal Interactions in Australian Residential Aged Care Facilities
by Wendy Newton, Tania Signal and Jenni A. Judd
Animals 2022, 12(24), 3591; https://doi.org/10.3390/ani12243591 - 19 Dec 2022
Cited by 1 | Viewed by 1714
Abstract
Animal-assisted interventions (AAI) have been occurring in Australian Residential Aged Care Facilities (RACF) for more than 40 years and may relieve loneliness and improve quality of life. The presence of animals in RACF poses an inherent risk to residents and the animals involved. [...] Read more.
Animal-assisted interventions (AAI) have been occurring in Australian Residential Aged Care Facilities (RACF) for more than 40 years and may relieve loneliness and improve quality of life. The presence of animals in RACF poses an inherent risk to residents and the animals involved. Little is known about the policies and guidelines for including animals in the Australian RACF. We anticipated that most RACFs would have some policies, but they may lack the detail necessary to keep humans and animals safe. Using an adapted survey, we surveyed and interviewed a small but representative sample of Australian RACF managers. The results demonstrated that RACF did have animal policies; however, the content regarding the need for hand washing, infection prevention, and animal welfare was lacking. Including unregulated family pets in RACF was an unexpected additional risk factor identified during data analysis. There is a need for national guidelines tied to the national aged care policy, which includes training and educational resources for RACF and AAI providers. Full article
(This article belongs to the Special Issue The Health Benefits of Human–Animal Interactions)
12 pages, 316 KiB  
Article
Environmental Effects and Genetic Parameters for Growth Traits of Lohi Sheep
by Numan Sharif, Asad Ali, Muhammad Dawood, Muhammad Irfan-ur-Rehman Khan and Duy Ngoc Do
Animals 2022, 12(24), 3590; https://doi.org/10.3390/ani12243590 - 19 Dec 2022
Cited by 2 | Viewed by 1763
Abstract
Estimating genetic parameters for growth traits is crucial to plan breeding strategies for improving meat production in indigenous sheep breeds. The study first tested the effects of environmental and maternal effects on five growth traits, including birth weight (BWT), weight at 120 days [...] Read more.
Estimating genetic parameters for growth traits is crucial to plan breeding strategies for improving meat production in indigenous sheep breeds. The study first tested the effects of environmental and maternal effects on five growth traits, including birth weight (BWT), weight at 120 days (WT120), weight at 180 days (WT180), weight at 270 days (WT270) and weight at 365 days of age (WT365) and then estimated genetic parameters for these traits using data obtained in 1215 Lohi sheep. The effects of factors, including year (YOB), season (SOB) and type of birth (TOB), age of dam (AOD) and sex on growth traits of Lohi sheep, were examined using analysis of variance (ANOVA) in R software. Sex, TOB and YOB significantly affected all studied traits. The estimates of direct and maternal heritability for BWT and WT120 were 0.15 ± 0.08 and 0.20 ± 0.06, and 0.45 ± 0.16, 0.21 ± 0.08, respectively. The direct heritability estimates for WT180, WT270 and WT365 were 0.20 ± 0.07, 0.21 ± 0.07 and 0.19 ± 0.08, respectively. Due to the high heritability estimate obtained for WT120 compared to other studied traits, and its strong genetic correlation (>0.9) with post-weaning growth traits, it is recommended that selection must be practiced on WT120 to improve the growth performance of Lohi sheep. The results could be used for the development of genetic/genomic selection programs aiming to improve the production performance of the Lohi sheep. Full article
(This article belongs to the Section Animal Genetics and Genomics)
16 pages, 822 KiB  
Review
Neurobiology of Maternal Behavior in Nonhuman Mammals: Acceptance, Recognition, Motivation, and Rejection
by Genaro A. Coria-Avila, Deissy Herrera-Covarrubias, Luis I. García, Rebeca Toledo, María Elena Hernández, Pedro Paredes-Ramos, Aleph A. Corona-Morales and Jorge Manzo
Animals 2022, 12(24), 3589; https://doi.org/10.3390/ani12243589 - 19 Dec 2022
Cited by 6 | Viewed by 3214
Abstract
Among the different species of mammals, the expression of maternal behavior varies considerably, although the end points of nurturance and protection are the same. Females may display passive or active responses of acceptance, recognition, rejection/fear, or motivation to care for the offspring. Each [...] Read more.
Among the different species of mammals, the expression of maternal behavior varies considerably, although the end points of nurturance and protection are the same. Females may display passive or active responses of acceptance, recognition, rejection/fear, or motivation to care for the offspring. Each type of response may indicate different levels of neural activation. Different natural stimuli can trigger the expression of maternal and paternal behavior in both pregnant or virgin females and males, such as hormone priming during pregnancy, vagino-cervical stimulation during parturition, mating, exposure to pups, previous experience, or environmental enrichment. Herein, we discuss how the olfactory pathways and the interconnections of the medial preoptic area (mPOA) with structures such as nucleus accumbens, ventral tegmental area, amygdala, and bed nucleus of stria terminalis mediate maternal behavior. We also discuss how the triggering stimuli activate oxytocin, vasopressin, dopamine, galanin, and opioids in neurocircuitries that mediate acceptance, recognition, maternal motivation, and rejection/fear. Full article
(This article belongs to the Special Issue Animal Perinatology: Behavior and Health of the Dam and Her Offspring)
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13 pages, 843 KiB  
Article
Application of QBA to Assess the Emotional State of Horses during the Loading Phase of Transport
by Francesca Dai, Maria Giorgia Riva, Emanuela Dalla Costa, Riccardo Pascuzzo, Alana Chapman and Michela Minero
Animals 2022, 12(24), 3588; https://doi.org/10.3390/ani12243588 - 19 Dec 2022
Viewed by 1438
Abstract
To identify feasible indicators to evaluate animals’ emotional states as a parameter to assess animal welfare, the present study aimed at investigating the accuracy of free choice profiling (FCP) and fixed list (FL) approach of Qualitative Behaviour Assessment (QBA) in horses during the [...] Read more.
To identify feasible indicators to evaluate animals’ emotional states as a parameter to assess animal welfare, the present study aimed at investigating the accuracy of free choice profiling (FCP) and fixed list (FL) approach of Qualitative Behaviour Assessment (QBA) in horses during the loading phase of transport. A total of 13 stakeholders were trained to score 2 different sets of videos of mixed breed horses loaded for road transport, using both FCP and FL, in 2 sessions. Generalized Procustes Analysis (GPA) consensus profile explained a higher percentage of variation (80.8%) than the mean of 1000 randomized profiles (41.2 ± 1.6%; p = 0.001) for the FCP method, showing an excellent inter-observer agreement. GPA identified two main factors, explaining 65.1% and 3.7% of the total variation. Factor 1 ranging from ‘anxious/ to ‘calm/relaxed’, described the valence of the horses’ emotional states. Factor 2, ranging from ‘bright’ to ‘assessing/withdrawn’, described the arousal. As for FL, Principal Component Analysis (PCA) first and second components (PC1 and PC2, respectively), explaining on average 59.8% and 12.6% of the data variability, had significant agreement between observers. PC1 ranges from relaxed/confident to anxious/frightened, while PC2 from alert/inquisitive to calm. Our study highlighted the need for the use of descriptors specifically selected, throughout a prior FCP process for the situation we want to evaluate to get a good QBA accuracy level. Full article
(This article belongs to the Special Issue Horse Transport)
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11 pages, 548 KiB  
Article
Alternative Forages as Roughage for Ruminant: Nutritional Characteristics and Digestibility of Six Exotic Plants in Azores Archipelago
by Helder P. B. Nunes, Sofia Teixeira, Cristiana S. A. M. Maduro Dias and Alfredo E. S. Borba
Animals 2022, 12(24), 3587; https://doi.org/10.3390/ani12243587 - 19 Dec 2022
Cited by 3 | Viewed by 1988
Abstract
This study aimed to evaluate the nutritional potential of unconventional plants: Pittosporum undulatum, Cryptomeria japonica, Acacia melanoxylon, Hedychium gardnerianum, Eucalyptus globulus, and Arundo donax, as an alternative roughage for ruminants. Chemical composition, gross energy, in vitro gas [...] Read more.
This study aimed to evaluate the nutritional potential of unconventional plants: Pittosporum undulatum, Cryptomeria japonica, Acacia melanoxylon, Hedychium gardnerianum, Eucalyptus globulus, and Arundo donax, as an alternative roughage for ruminants. Chemical composition, gross energy, in vitro gas production, kinetics, and digestibility of dry matter and organic matter in vitro were determined for each species. The obtained results showed variations between the studied forages concerning crude protein, and the different fiber fractions: NDF, ADF, and ADL The P. undulatum with a relative food value of 92.12%, showed a significant difference compared to the other species under study. After 96 h of incubation, the plants that produced, on average, less in vitro gas were A. melanoxylon and E. globulus. Among the studied species, A. donax stands out as the species that presented the highest gas production, with 31.53 mL. 200 mg−1 DM, observing a significant difference compared to the other plants. This is a reflection of it having the highest DMD (60.44 ± 1.22%) as well. P. undulatum was the species with the longest colonization time (4.8 h). Among the plants studied, we highlight P. undulatum as presenting a good quality in the RFV index and A. donax as having good digestibility. Both can be used as roughage in periods of greater shortage of pastures. Full article
(This article belongs to the Collection Novel Animal Feeds, Forage Crops, and Cultivation Strategies for Enhancing Livestock Nutrition and Ecosystem Services)
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17 pages, 2775 KiB  
Article
Genomic Characterization of hox Genes in Senegalese Sole (Solea senegalensis, Kaup 1858): Clues to Evolutionary Path in Pleuronectiformes
by Marco Mendizábal-Castillero, Manuel Alejandro Merlo, Ismael Cross, María Esther Rodríguez and Laureana Rebordinos
Animals 2022, 12(24), 3586; https://doi.org/10.3390/ani12243586 - 19 Dec 2022
Cited by 1 | Viewed by 1592
Abstract
The Senegalese sole (Solea senegalensis, Kaup 1858), a marine flatfish, belongs to the Pleuronectiformes order. It is a commercially important species for fisheries and aquaculture. However, in aquaculture, several production bottlenecks have still to be resolved, including skeletal deformities and high [...] Read more.
The Senegalese sole (Solea senegalensis, Kaup 1858), a marine flatfish, belongs to the Pleuronectiformes order. It is a commercially important species for fisheries and aquaculture. However, in aquaculture, several production bottlenecks have still to be resolved, including skeletal deformities and high mortality during the larval and juvenile phase. The study aims to characterize the hox gene clusters in S. senegalensis to understand better the developmental and metamorphosis process in this species. Using a BAC library, the clones that contain hox genes were isolated, sequenced by NGS and used as BAC-FISH probes. Subsequently the hox clusters were studied by sequence analysis, comparative genomics, and cytogenetic and phylogenetic analysis. Cytogenetic analysis demonstrated the localization of four BAC clones on chromosome pairs 4, 12, 13, and 16 of the Senegalese sole cytogenomic map. Comparative and phylogenetic analysis showed a highly conserved organization in each cluster and different phylogenetic clustering in each hox cluster. Analysis of structural and repetitive sequences revealed accumulations of polymorphisms mediated by repetitive elements in the hoxba cluster, mainly retroelements. Therefore, a possible loss of the hoxb7a gene can be established in the Pleuronectiformes lineage. This work allows the organization and regulation of hox clusters to be understood, and is a good base for further studies of expression patterns. Full article
(This article belongs to the Special Issue Genetic and Genomic Advances in Fisheries and Aquaculture)
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12 pages, 1924 KiB  
Article
Estimation of Genetic Parameters of Heat Tolerance for Production Traits in Canadian Holsteins Cattle
by Ivan L. Campos, Tatiane C. S. Chud, Gerson A. Oliveira Junior, Christine F. Baes, Ángela Cánovas and Flavio S. Schenkel
Animals 2022, 12(24), 3585; https://doi.org/10.3390/ani12243585 - 19 Dec 2022
Cited by 3 | Viewed by 2039
Abstract
Understanding how cows respond to heat stress has helped to provide effective herd management practices to tackle this environmental challenge. The possibility of selecting animals that are genetically more heat tolerant may provide additional means to maintain or even improve the productivity of [...] Read more.
Understanding how cows respond to heat stress has helped to provide effective herd management practices to tackle this environmental challenge. The possibility of selecting animals that are genetically more heat tolerant may provide additional means to maintain or even improve the productivity of the Canadian dairy industry, which is facing a shifting environment due to climate changes. The objective of this study was to estimate the genetic parameters for heat tolerance of milk, fat, and protein yields in Canadian Holstein cows. A total of 1.3 million test-day records from 195,448 first-parity cows were available. A repeatability test-day model fitting a reaction norm on the temperature-humidity index (THI) was used to estimate the genetic parameters. The estimated genetic correlations between additive genetic effect for production and for heat tolerance ranged from −0.13 to −0.21, indicating an antagonistic relationship between the level of production and heat tolerance. Heritability increased marginally as THI increased above its threshold for milk yield (0.20 to 0.23) and protein yield (0.14 to 0.16) and remained constant for fat yield (0.17). A Spearman rank correlation between the estimated breeding values under thermal comfort and under heat stress showed a potential genotype by environmental interaction. The existence of a genetic variability for heat tolerance allows for the selection of more heat tolerant cows. Full article
(This article belongs to the Special Issue Effects of Heat Stress on Livestock and Adaptation Methods)
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13 pages, 2198 KiB  
Article
Comprehensive Analyses of the Bacterial Population in Non-Healing Claw Lesions of Dairy Cattle
by Kaoru Hori, Takako Taniguchi, Trigan Elpita, Rathanon Khemgaew, Satomi Sasaki, Yasuhiro Gotoh, Ichiro Yasutomi and Naoaki Misawa
Animals 2022, 12(24), 3584; https://doi.org/10.3390/ani12243584 - 18 Dec 2022
Viewed by 1704
Abstract
Non-healing claw lesions (NHCLs) are a newly characterized disorder affecting the deep dermis of the hoof in dairy cattle. Although NHCLs are thought to be associated with bovine digital dermatitis (BDD), their precise etiology is not yet understood. To investigate the bacterial populations [...] Read more.
Non-healing claw lesions (NHCLs) are a newly characterized disorder affecting the deep dermis of the hoof in dairy cattle. Although NHCLs are thought to be associated with bovine digital dermatitis (BDD), their precise etiology is not yet understood. To investigate the bacterial populations present in each type of NHCL (toe necrosis: TN, non-healing white line disease: nhWLD, and a non-healing sole ulcer: nhSU), and the newly added entity non-healing verrucous-like lesions (nhVLL), 16S rRNA-based metagenomic analysis with next-generation sequencing (NGS) was employed. Twelve cases of NHCLs (3 TN, 3 nhWLD, 4 nhSU, and 2 nhVLL) were collected from five dairy farms in two prefectures in Japan. Three samples of healthy hoof dermis collected from two farms and a slaughterhouse were used as controls. Furthermore, culture-dependent and -independent approaches were conducted for detecting Treponema species and Fusobacterium necrophorum. As reported in BDD, Treponema species and F. necrophorum were detected frequently from NHCLs by PCR and immunohistochemistry, but NGS showed that these bacterial genera were not predominant in NHCLs. The predominant bacterial genera in NHCLs differed among the lesions examined, suggesting that Treponema species present predominantly in BDD were not predominant in NHCLs and that the bacterial population in NHCLs may vary among individual cattle and/or farms. Full article
(This article belongs to the Special Issue Bovine Infectious Disease and Immunity)
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16 pages, 1293 KiB  
Article
Establishment of Hematological and Plasma Biochemical Reference Values and Analysis of Risk Factors for Pet Sugar Gliders (Petaurus breviceps) in Taiwan
by Vivian C. Y. Lin, Ning-Ya Yang, Wen-Chi Lin, Jo-Wen Chen, Ching-Yi Yen and Yi-Lun Tsai
Animals 2022, 12(24), 3583; https://doi.org/10.3390/ani12243583 - 18 Dec 2022
Cited by 1 | Viewed by 3367
Abstract
Hematological and plasma biochemical examination are crucial in the veterinary care of sugar gliders, which are increasingly popular in Taiwan and the United States. However, published research of the species’ reference interval and related influencing factors were rare. The objectives of this study [...] Read more.
Hematological and plasma biochemical examination are crucial in the veterinary care of sugar gliders, which are increasingly popular in Taiwan and the United States. However, published research of the species’ reference interval and related influencing factors were rare. The objectives of this study were to establish the hematological and plasma biochemical reference values for captive sugar gliders in Taiwan and to evaluate the influence of factors including age, gender, neuter status, location, season, diet, caging arrangement, and other pets in the household. A total of 42 clinically healthy pet sugar gliders were recruited. Morphometrical measurements and physiological data were collected, and hematological and plasma biochemical examinations were performed. The reference value of each index was calculated using Reference Value Advisor (RVA) software, following the American Society for Veterinary Clinical Pathology (ASVCP) guidelines. Normality of data distribution was tested, and data transformation was conducted. The parametric method and robust method were used to determine reference limits. Univariate analysis was performed, and multiple regression models were built for each hematological and plasma biochemical parameter. Red blood cell, hematocrit, and hemoglobin levels were higher in males, compared to females, while they were lower in the neutered group, compared to the intact group. Relative neutrophil counts were higher in elder sugar gliders, while relative lymphocyte counts were lower. Aspartate transaminase levels were higher in elder sugar gliders, while albumin levels were lower. Blood urea nitrogen levels were highest in spring. The blood profile and related effects presented in this study can provide useful information for veterinary care in pet sugar gliders. Full article
(This article belongs to the Special Issue Hematology and Transfusion Medicine in Animals)
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17 pages, 2198 KiB  
Review
Approach to Small Animal Neurorehabilitation by Locomotor Training: An Update
by Débora Gouveia, Ana Cardoso, Carla Carvalho, António Almeida, Óscar Gamboa, António Ferreira and Ângela Martins
Animals 2022, 12(24), 3582; https://doi.org/10.3390/ani12243582 - 18 Dec 2022
Cited by 3 | Viewed by 2562
Abstract
Neurorehabilitation has a wide range of therapies to achieve neural regeneration, reorganization, and repair (e.g., axon regeneration, remyelination, and restoration of spinal circuits and networks) to achieve ambulation for dogs and cats, especially for grade 1 (modified Frankel scale) with signs of spinal [...] Read more.
Neurorehabilitation has a wide range of therapies to achieve neural regeneration, reorganization, and repair (e.g., axon regeneration, remyelination, and restoration of spinal circuits and networks) to achieve ambulation for dogs and cats, especially for grade 1 (modified Frankel scale) with signs of spinal shock or grade 0 (deep pain negative), similar to humans classified with ASIA A lesions. This review aims to explain what locomotor training is, its importance, its feasibility within a clinical setting, and some possible protocols for motor recovery, achieving ambulation with coordinated and modulated movements. In addition, it cites some of the primary key points that must be present in the daily lives of veterinarians or rehabilitation nurses. These can be the guidelines to improve this exciting exercise necessary to achieve ambulation with quality of life. However, more research is essential in the future years. Full article
(This article belongs to the Special Issue Clinical Research Advances in Intensive Neurorehabilitation in Companion Animals)
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14 pages, 2195 KiB  
Review
Tattoo Skin Disease in Cetacea: A Review, with New Cases for the Northeast Pacific
by Marie-Françoise Van Bressem, Koen Van Waerebeek and Pádraig J. Duignan
Animals 2022, 12(24), 3581; https://doi.org/10.3390/ani12243581 - 18 Dec 2022
Cited by 4 | Viewed by 2253
Abstract
Tattoo skin disease (TSD) is a poxviral dermatopathy diagnosed in cetaceans. We review the literature on TSD aetiology, clinical characteristics, pathology and epidemiology and evaluate immune responses against the virus. In addition, necropsy reports for fifty-five harbour porpoises (Phocoena phocoena), twenty-two [...] Read more.
Tattoo skin disease (TSD) is a poxviral dermatopathy diagnosed in cetaceans. We review the literature on TSD aetiology, clinical characteristics, pathology and epidemiology and evaluate immune responses against the virus. In addition, necropsy reports for fifty-five harbour porpoises (Phocoena phocoena), twenty-two Delphinidae and four Kogiidae stranded in northern California in 2018–2021 were checked for diagnostic tattoo lesions. TSD occurs in the Mediterranean, North and Barents Seas, as well as in the Atlantic, eastern Pacific and Indian Oceans in at least 21 cetacean species, with varying prevalence. Two cetacean poxvirus (CePV) clades are recognised: CePV-1 in odontocetes and CePV-2 in mysticetes. CePV-1 isolates were recovered from six Delphinidae and one Phocoenidae in the Americas, Europe and Hong Kong. Strains from Delphinidae are closely related. Among Phocoenidae, poxviruses were sampled only in harbour porpoises around the British Isles. CePV-2 isolates were obtained from southern right whales (Eubalaena australis) and a bowhead whale (Balaena mysticetus). In healthy animals, an immune response develops over time, with young calves protected by maternal immunity. Salinity and sea surface temperature do not seem to influence TSD prevalence in free-ranging cetaceans. High concentrations of immunotoxic halogenated organochlorines may cause a more severe clinical disease. Substitution and loss of genes involved in anti-viral immunity may favour CePV entry, replication and persistence in the epidermis. Off California, Delphinidae were less often (26.3%) affected by TSD than harbour porpoises (43.6%). Male porpoises were significantly more prone (58.1%) to show clinical disease than females (25%). Among males, TSD affected a high proportion of juveniles and subadults. TSD was not detected in the Kogiidae. Full article
(This article belongs to the Special Issue Frontiers in Marine Mammal Health and Immunity)
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12 pages, 279 KiB  
Article
Plasma Amino Acid Concentration in Obese Horses with/without Insulin Dysregulation and Laminitis
by Sabita Diana Stoeckle, Detlef Timmermann, Roswitha Merle and Heidrun Gehlen
Animals 2022, 12(24), 3580; https://doi.org/10.3390/ani12243580 - 18 Dec 2022
Viewed by 1519
Abstract
Laminitic horses commonly suffer from an endocrine disease such as equine metabolic syndrome. Hyperinsulinemia is considered a key factor in the pathogenesis of laminitis. Since insulin also affects protein turnover in the body, the resting plasma amino acid concentrations of obese horses that [...] Read more.
Laminitic horses commonly suffer from an endocrine disease such as equine metabolic syndrome. Hyperinsulinemia is considered a key factor in the pathogenesis of laminitis. Since insulin also affects protein turnover in the body, the resting plasma amino acid concentrations of obese horses that were presented for a combined glucose insulin test (CGIT) were determined. In total, 25 obese horses and two lean horses with recurrent laminitis underwent a CGIT. Of these, five were not insulin dysregulated (obese), 14 were insulin dysregulated (ID), and eight were insulin-dysregulated and laminitic (IDL). Significant differences in the resting concentrations between obese and insulin dysregulated and laminitic (citrulline p = 0.038, obese: 73.001 ± 12.661 nmol/mL, IDL: 49.194 ± 15.486 nmol/mL; GABA p = 0.02, obese: 28.234 ± 3.885 nmol/mL, IDL: 16.697 ± 1.679 nmol/mL; methionine p = 0.018, obese: 28.691 ± 5.913 nmol/mL, IDL: 20.143 ± 3.09 nmol/mL) as well as between insulin dysregulated individuals with and without laminitis (GABA p < 0.001, ID: 28.169 ± 6.739 nmol/mL) regarding three amino acids were determined. This may be an interesting approach, especially for diagnostic testing and possibly also for the feed supplements of horses at risk of developing laminitis. However, further research, including a higher number of cases, is required. Full article
(This article belongs to the Special Issue Advances in Equine Metabolomics)
16 pages, 1351 KiB  
Article
Effect of Rice Protein Meal Replacement of Fish Meal on Growth, Anti-Oxidation Capacity, and Non-Specific Immunity for Juvenile Shrimp Litopenaeus vannamei
by Huaxing Lin, Shuqing He, Beiping Tan, Xiaomin Zhang, Yi Lin and Qihui Yang
Animals 2022, 12(24), 3579; https://doi.org/10.3390/ani12243579 - 17 Dec 2022
Cited by 3 | Viewed by 2541
Abstract
This study assessed the effect of rice protein meal replacement for fish meal on the growth, nonspecific immunity, and disease resistance on juvenile shrimp Litopenaeus vannamei. Six groups of iso-nitrogenous and iso-lipid feeds named FM, R10, R20, R40, R60, and R80 were [...] Read more.
This study assessed the effect of rice protein meal replacement for fish meal on the growth, nonspecific immunity, and disease resistance on juvenile shrimp Litopenaeus vannamei. Six groups of iso-nitrogenous and iso-lipid feeds named FM, R10, R20, R40, R60, and R80 were prepared by replacing 0%, 10%, 20%, 40%, 60%, and 80% in FM protein with RPM, respectively, and then fed to the shrimps (0.54 ± 0.01 g). An amount of 720 healthy and evenly sized shrimps were allocated to six groups (three replicates per group) and fed four times a day (7:00, 11:00, 17:00 and 21:00) for eight weeks. Results revealed no significant differences in WG, FCR, and SGR of shrimps after replacing FM with 10% RPM (p > 0.05). In the R10 and R20 groups, SOD and T-AOC activities were significantly higher than those in the FM group, whereas the opposite was observed for MDA content (p < 0.05). CAT, ACP, and LZM were all significantly higher in the R10, R20, and R40 groups than in the FM group (p < 0.05). GSH-Px activity in the R10 group was significantly higher than the activity in the FM group (p < 0.05). AKP, PO, TYS, GPT, and GOT activities were significantly higher in the R10 group than in the FM group (p < 0.05). Compared to the FM group, the eukaryotic translation initiation factor 3K (eif3k) gene was significantly up-regulated in the R10 group, whereas the penaiedin 3a (pen 3a) and anti-lipopolysaccharide factor (alf) genes were significantly up-regulated in the R10 and R20 groups (p < 0.05). The crustin a (cru a), immune deficiency (imd), and lysozyme (lzm) mRNA levels were significantly higher in the R10, R20, and R40 groups than in the other groups (p < 0.05). The prophenoloxidase (PO) mRNA levels in the R20 group were significantly higher than those in the FM group (p < 0.05). The replacement of 10–40% of FM with RPM improved the gut flora composition of shrimps, increasing beneficial bacteria (Bacteroidetes) abundance and reducing harmful bacteria (Aspergillus and Vibrio) abundance. After the challenge test of Vibrio parahaemolyticus (7 days), the cumulative mortality in the R10 group significantly decreased (p < 0.05). In conclusion, replacement of 10% FM by RPM significantly improved digestibility, protein synthesis, antioxidant capacity, and disease resistance in L. vannamei. Full article
(This article belongs to the Special Issue Alternative Feedstuffs, Functional Foods and Feed Additives for Aquatic Organisms)
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11 pages, 891 KiB  
Article
Differences in Weight, Hierarchy, and Incidence of Lameness between Two Groups of Adult Pigs Derived from Assisted Reproductive Technologies
by Jon Romero-Aguirregomezcorta, Lisette L. Ramírez, Alba Ortín, Guillermo Ramis, Raquel Romar and Pilar Coy
Animals 2022, 12(24), 3578; https://doi.org/10.3390/ani12243578 - 17 Dec 2022
Cited by 1 | Viewed by 1561
Abstract
The in vitro production (IVP) and subsequent transfer of embryos (ET) to recipient mothers is not yet an established reproductive technology in the pig industry, as it is in cattle. However, that the trade of IVP-cryopreserved pig embryos is expected to start in [...] Read more.
The in vitro production (IVP) and subsequent transfer of embryos (ET) to recipient mothers is not yet an established reproductive technology in the pig industry, as it is in cattle. However, that the trade of IVP-cryopreserved pig embryos is expected to start in the next decades. Society and governments are increasingly aware of the repercussions that IVP could have for animal health, welfare, behavior, or food safety, but proven scientific information for this type of animal does not exist, since no colonies of pigs have been created to this end. We created a small one and studied the differences between 16 IVP-derived pigs and 14 pigs derived from artificial insemination (AI), at 3.5 years of age, conceived from the same boar, and housed and fed under the same conditions since they were born. Incidence of lameness, position in the herd hierarchy, weight, adenosine deaminase activity, and hematological and biochemical analytes were compared between the two groups of animals. The results showed that the IVP animals weighed more, occupied higher positions in the herd hierarchy, and had a lower incidence of lameness. Although genetic differences from the maternal line could explain some of these results, it is also possible that the IVP animals developed better adaptative abilities, but more studies with a higher number of animals are necessary to reach consistent conclusions. Full article
(This article belongs to the Section Animal Reproduction)
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10 pages, 539 KiB  
Article
Flock Factors Correlated with Elevated Mortality in Non-Beak Trimmed Aviary-Housed Layers
by Käthe Elise Kittelsen, Fernanda Tahamtani, Randi Oppermann Moe, Pall Gretarsson and Guro Vasdal
Animals 2022, 12(24), 3577; https://doi.org/10.3390/ani12243577 - 17 Dec 2022
Cited by 2 | Viewed by 1432
Abstract
The use of non-cage housing systems for layers is increasing in Europe and elsewhere. Knowledge of factors that may affect mortality in these systems is important to be able to improve animal welfare, reduce mortality and enhance sustainability. The aim of this study [...] Read more.
The use of non-cage housing systems for layers is increasing in Europe and elsewhere. Knowledge of factors that may affect mortality in these systems is important to be able to improve animal welfare, reduce mortality and enhance sustainability. The aim of this study was to investigate factors that may contribute to increased mortality in non-beak trimmed aviary-housed laying hens in Norway. A total of 39 non-beak trimmed commercial flocks (Lohmann LSL (n = 25) and Dekalb White (n = 14)) were visited between week 70 to 76 of life, and factors related to health, behaviour and management were recorded. Mean mortality in the flocks was 3% (range: 0.5–9%) and increased flock mortality was correlated with total feather loss (p < 0.05); feather loss on the breast (p < 0.02) and feather loss on the head (p < 0.003). There was an association between layer hybrid line and mortality (p = 0.055). Furthermore, a low positive correlation between mortality and dust level inside the barn was found (p < 0.04), showing that mortality was higher when dust level was also high. No correlation between mortality and the provision of environmental enrichment was found. In conclusion, this study found an association between flocks with elevated mortality (>3.0%) and increased feather loss which may indicate feather pecking. The results underline the importance of regularly assessment of plumage condition in commercial layer farms, as a tool to detect early signs of feather pecking in commercial aviary-housed layer flocks. This may help to target feather pecking before cannibalism breaks out. Full article
(This article belongs to the Section Poultry)
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13 pages, 2053 KiB  
Article
Immunodetection of P2X2 Receptor in Enteric Nervous System Neurons of the Small Intestine of Pigs
by Sylwia Mozel and Marcin B. Arciszewski
Animals 2022, 12(24), 3576; https://doi.org/10.3390/ani12243576 - 17 Dec 2022
Viewed by 1334
Abstract
Extracellular adenosine 5′-triphosphate (ATP) is one of the best-known and frequently studied neurotransmitters. Its broad spectrum of biological activity is conditioned by the activation of purinergic receptors, including the P2X2 receptor. The P2X2 receptor is present in the central and peripheral nervous system [...] Read more.
Extracellular adenosine 5′-triphosphate (ATP) is one of the best-known and frequently studied neurotransmitters. Its broad spectrum of biological activity is conditioned by the activation of purinergic receptors, including the P2X2 receptor. The P2X2 receptor is present in the central and peripheral nervous system of many species, including laboratory animals, domestic animals, and primates. However, the distribution of the P2X2 receptor in the nervous system of the domestic pig, a species increasingly used as an experimental model, is as yet unknown. Therefore, this study aimed to determine the presence of the P2X2 receptor in the neurons of the enteric nervous system (ENS) of the pig small intestine (duodenum, jejunum, and ileum) by the immunofluorescence method. In addition, the chemical code of P2X2-immunoreactive (IR) ENS neurons of the porcine small intestine was analysed by determining the coexistence of selected neuropeptides, i.e., vasoactive intestinal polypeptide (VIP), substance P (sP), and galanin. P2X2-IR neurons were present in the myenteric plexus (MP), outer submucosal plexus (OSP), and inner submucosal plexus (ISP) of all sections of the small intestine (duodenum, jejunum, and ileum). From 44.78 ± 2.24% (duodenum) to 63.74 ± 2.67% (ileum) of MP neurons were P2X2-IR. The corresponding ranges in the OSP ranged from 44.84 ± 1.43% (in the duodenum) to 53.53 ± 1.21% (in the jejunum), and in the ISP, from 53.10 ± 0.97% (duodenum) to 60.57 ± 2.24% (ileum). Immunofluorescence staining revealed the presence of P2X2-IR/galanin-IR and P2X2-IR/VIP-IR neurons in the MP, OSP, and ISP of the sections of the small intestine. The presence of sP was not detected in the P2X2-IR neurons of any ganglia tested in the ENS. Our results indicate for the first time that the P2X2 receptor is present in the MP, ISP, and OSP neurons of all small intestinal segments in pigs, which may suggest that its activation influences the action of the small intestine. Moreover, there is a likely functional interaction between P2X2 receptors and galanin or VIP, but not sP, in the ENS of the porcine small intestine. Full article
(This article belongs to the Special Issue Microscopic Structure Research in Animals)
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12 pages, 1314 KiB  
Article
From Extermination to Conservation: Historical Records of Shark Presence during the Early and Development Phase of the Greek Fishery
by Dimitrios K. Moutopoulos, Evridiki Lazari, George Katselis and Ioannis Giovos
Animals 2022, 12(24), 3575; https://doi.org/10.3390/ani12243575 - 17 Dec 2022
Cited by 1 | Viewed by 3160
Abstract
The lack of historical data on shark presence, distribution, and status in the Eastern Mediterranean undermines efforts to manage and protect their populations. An exhaustive review of anecdotal references related to shark presence during the early and development phase of Greek fisheries (1883–1983) [...] Read more.
The lack of historical data on shark presence, distribution, and status in the Eastern Mediterranean undermines efforts to manage and protect their populations. An exhaustive review of anecdotal references related to shark presence during the early and development phase of Greek fisheries (1883–1983) was conducted. In the early-20th century (1912), the first sighting of the presence of a dead shark was reported in the Ionian Sea. Later on, the presence of sharks gradually increased up to 1969, with most records being more frequent for the Aegean Sea, whereas the number of sharks being sighted declined leading up to the middle of 1980s. The increase in shark attacks during the mid-20th century led to a calling for culling of sharks in co-operation with the competent authorities promoting the permission to hunt sharks with firearms and offering rewards for killed individuals. A high number of these observations potentially resulted from shark attacks on people, whereas this is not currently evident. This is an indicator of the lower abundance of sharks in modern times and subsequently an alteration in the way that our current modern society is approaching the protection of such vulnerable species. Full article
(This article belongs to the Special Issue Sharks and Skates: Ecology, Distribution and Conservation)
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16 pages, 3402 KiB  
Article
Feed Supplemented with Aronia melanocarpa (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors
by Bo Jing, Huanwei Xiao, Haixu Yin, Yingbo Wei, Haoyuan Wu, Dongliang Zhang, Ivan Stève Nguepi Tsopmejio, Hongmei Shang, Zhouyu Jin and Hui Song
Animals 2022, 12(24), 3574; https://doi.org/10.3390/ani12243574 - 17 Dec 2022
Cited by 1 | Viewed by 1808
Abstract
The continuous ovulation of laying hens during the peak period is likely to cause oxidative stress, resulting in a reduction in the laying cycle over time. The aim of this study was to evaluate the antioxidant effects of Aronia melanocarpa (AM) in the [...] Read more.
The continuous ovulation of laying hens during the peak period is likely to cause oxidative stress, resulting in a reduction in the laying cycle over time. The aim of this study was to evaluate the antioxidant effects of Aronia melanocarpa (AM) in the diet and its effect on the yolk precursor content caused by ovulation in laying hens during the peak period. A total of 300 25-week-old Roman brown laying hens were randomly divided into five groups with six replicates in each group, 10 in each replicate. The control group was fed a basal diet, the positive control group was fed a Vitamin C (VC) plus basal diet, and the experimental group was fed 1%, 4%, and 7% doses of AM plus diet according to the principle of energy and nitrogen requirements, which lasted eight weeks. At the end of the study, the egg quality, biochemical, and antioxidant markers, as well as mRNA and protein expressions, were evaluated to determine the potential signaling pathways involved. Results showed that the addition of AM to the feed increased the weight of laying hens at the peak of egg production and improved egg quality. The biochemical markers, as well as the antioxidant parameters in the serum, liver, and ovarian tissues, were ameliorated. The gene and protein expression of recombinant kelch-like ECH-associated protein 1 (Keap1) in the liver and ovarian tissues was decreased, while nuclear factor erythroid-2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression was increased. The feed supplemented with AM also increased the estrogen contents and lipid parameters, as well as the gene and protein expressions related to the yolk precursor. Feed supplemented with AM could improve the egg quality and the oxidative stress caused by the ovulation process of laying hens during the peak egg production period by activating the Keap1/Nrf2 signaling pathway. These results suggest that the feed supplemented with 1% AM and 4% AM can improve egg production in peak laying hens. Full article
(This article belongs to the Special Issue The Use of Phytogenic Feed Additives to Enhance Productivity and Health in Animals)
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12 pages, 6326 KiB  
Article
Clinical Presentation, MRI Characteristics, and Outcome of Conservative or Surgical Management of Spinal Epidural Empyema in 30 Dogs
by Carlos Blanco, Meritxell Moral, Juan José Minguez and Valentina Lorenzo
Animals 2022, 12(24), 3573; https://doi.org/10.3390/ani12243573 - 17 Dec 2022
Viewed by 1699
Abstract
Spinal epidural empyema (SEE) represents a neurological emergency in veterinary medicine, but information on this condition is limited to date. This retrospective case series study describes the clinical and magnetic resonance imaging (MRI) features, and the outcome of conservative or surgical management of [...] Read more.
Spinal epidural empyema (SEE) represents a neurological emergency in veterinary medicine, but information on this condition is limited to date. This retrospective case series study describes the clinical and magnetic resonance imaging (MRI) features, and the outcome of conservative or surgical management of SEE in 30 dogs diagnosed with SEE from September 2015 to March 2020 at one referral neurology centre. The most frequent clinical sign was pain 28/30 (93%), and 22/30 (73%) showed neurological signs with ambulatory paraparesis/tetraparesis 15/30 (50%), monoparesis 1/30 (3.3%), non-ambulatory paraparesis 3/30 (10%), or paraplegia 3/30 (10%). MRI was valuable for the diagnosis and in the follow-up. In this group of dogs, 24/30 (80%) were conservatively treated and 6/30 (20%) were surgically treated. The outcome was considered favourable in all dogs: 20/30 (66.6%) achieved full recovery (3 surgically treated and 17 medically treated) and 10/30 (33.3%) dogs had an improvement in the neurological signs with residual ambulatory paresis (3 surgically treated and 7 medically treated). Surgical treatment showed better short-term (7 days) outcomes than medical treatment in non-ambulatory paraparetic or paraplegic dogs (33%). Nevertheless, this study suggests that a good recovery may be achieved with conservative treatment even for non-ambulatory or paraplegic dogs. Further prospective studies, with a standardised protocol of diagnostic tests and a homogeneous distribution of conservatively and surgically treated dogs, are needed to establish treatment guidelines. Full article
(This article belongs to the Special Issue Advances in Veterinary Neurology: From Diagnosis to Treatment)
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15 pages, 4270 KiB  
Article
Convergent Genomic Signatures of High-Altitude Adaptation among Six Independently Evolved Mammals
by Tianshu Lyu, Shengyang Zhou, Jiaohui Fang, Lidong Wang, Lupeng Shi, Yuehuan Dong and Honghai Zhang
Animals 2022, 12(24), 3572; https://doi.org/10.3390/ani12243572 - 16 Dec 2022
Cited by 1 | Viewed by 1914
Abstract
The species living in the Qinghai-Tibet Plateau provide an excellent model system for studying the relationship between molecular convergent evolution and adaptation. Distant species experiencing the same selection pressure (i.e., hypoxia, low temperature and strong ultraviolet radiation) are likely to evolve similar genetic [...] Read more.
The species living in the Qinghai-Tibet Plateau provide an excellent model system for studying the relationship between molecular convergent evolution and adaptation. Distant species experiencing the same selection pressure (i.e., hypoxia, low temperature and strong ultraviolet radiation) are likely to evolve similar genetic adaptations independently. Here, we performed comparative genomics studies on six independently evolved high-altitude species. The results also showed that the convergent evolution of the six species was mainly reflected at the level of rapidly evolving genes, and the functions of these rapidly evolving genes were mainly related to hypoxia response and DNA damage repair. In addition, we found that high-altitude species had more gene family changes than their low-altitude relatives, except for the order Lagomorpha. The results also show that the convergence of the gene family contraction of high-altitude species is much greater than that of expansion, revealing a possible pattern of species in adapting to high-altitude. Furthermore, we detected a positive selection signature in four genes related to hypoxia response and ultraviolet radiation damage in these six species (FYCO1, ERBIN, SCAMP1 and CXCL10). Our study reveals that hypoxia response might play an important role in the adaptation of independently evolved species to a high-altitude environment, providing a basic perspective for further exploring the high-altitude adaptation mechanism of different related species in the future. Full article
(This article belongs to the Section Mammals)
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14 pages, 3650 KiB  
Article
Rubbing Salt in the Wound: Molecular Evolutionary Analysis of Pain-Related Genes Reveals the Pain Adaptation of Cetaceans in Seawater
by Xiaoyue Ding, Fangfang Yu, Xiaofang He, Shixia Xu, Guang Yang and Wenhua Ren
Animals 2022, 12(24), 3571; https://doi.org/10.3390/ani12243571 - 16 Dec 2022
Viewed by 6702
Abstract
Pain, usually caused by a strong or disruptive stimulus, is an unpleasant sensation that serves as a warning to organisms. To adapt to extreme environments, some terrestrial animals have evolved to be inherently insensitive to pain. Cetaceans are known as supposedly indifferent to [...] Read more.
Pain, usually caused by a strong or disruptive stimulus, is an unpleasant sensation that serves as a warning to organisms. To adapt to extreme environments, some terrestrial animals have evolved to be inherently insensitive to pain. Cetaceans are known as supposedly indifferent to pain from soft tissue injury representatives of marine mammals. However, the molecular mechanisms that explain how cetaceans are adapted to pain in response to seawater environment remain unclear. Here, we performed a molecular evolutionary analysis of pain-related genes in selected representatives of cetaceans. ASIC4 gene was identified to be pseudogenized in all odontocetes (toothed whales) except from Physeter macrocephalus (sperm whales), and relaxed selection of this gene was detected in toothed whales with pseudogenized ASIC4. In addition, positive selection was detected in pain perception (i.e., ASIC3, ANO1, CCK, and SCN9A) and analgesia (i.e., ASIC3, ANO1, CCK, and SCN9A) genes among the examined cetaceans. In this study, potential convergent amino acid substitutions within predicted proteins were found among the examined cetaceans and other terrestrial mammals, inhabiting extreme environments (e.g., V441I of TRPV1 in cetaceans and naked mole rats). Moreover, specific amino acid substitutions within predicted sequences of several proteins were found in the studied representatives of cetaceans (e.g., F56L and D163A of ASIC3, E88G of GRK2, and F159L of OPRD1). Most of the substitutions were located within important functional domains of proteins, affecting their protein functions. The above evidence suggests that cetaceans might have undergone adaptive molecular evolution in pain-related genes through different evolutionary patterns to adapt to pain, resulting in greater sensitivity to pain and more effective analgesia. This study could have implications for diagnosis and treatment of human pain. Full article
(This article belongs to the Special Issue Adaptive Evolution and Trait Formation of Animals)
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20 pages, 6381 KiB  
Article
The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus
by Yan Qi, Hui-Liang Xue, Yun-Jiao Zheng, Yun-Fei Yin, Wen-Lei Xu, Jin-Hui Xu, Ming Wu, Lei Chen and Lai-Xiang Xu
Animals 2022, 12(24), 3570; https://doi.org/10.3390/ani12243570 - 16 Dec 2022
Viewed by 1482
Abstract
The photoperiod regulates the seasonal reproduction of mammals by affecting the follicle development, for which the granulosa cells provide nutrition. However, the underlying mechanism remains unclear. Here, Djungarian hamsters (Phodopus sungorus) were raised under different photoperiods to study the ovarian status [...] Read more.
The photoperiod regulates the seasonal reproduction of mammals by affecting the follicle development, for which the granulosa cells provide nutrition. However, the underlying mechanism remains unclear. Here, Djungarian hamsters (Phodopus sungorus) were raised under different photoperiods to study the ovarian status and explore the potential mechanism of the follicle development mediated by the FSH-Nodal/ALK7 signaling pathway. Compared with the moderate daylight (MD) group, the short daylight (SD) group exhibited a significant decrease in the ovarian weight and increase in the atretic follicle number and granulosa cell apoptosis, whereas the long daylight (LD) group showed an increase in the ovarian weight, the growing follicle number, and the antral follicle number, but a decrease in the granulosa cell apoptosis. Based on these findings, the key genes of the Nodal/ALK7 signaling pathway controlling the granulosa cell apoptosis were studied using the quantitative real-time polymerase chain reaction and western blotting. In the SD group, the follicle-stimulating hormone (FSH) concentration significantly decreased and the Nodal/ALK7/Smad signaling pathways were activated, while the phosphatidylinositol 3-kinase (PIK3)/Akt signaling pathway was inhibited. The BAX expression was significantly increased, while the Bcl-xL expression was significantly decreased, leading to an increase in the caspase-3 activity, the granulosa cell apoptosis, and ovarian degeneration. However, in the LD group, the FSH concentration significantly increased, the Nodal/ALK7/Smad signaling pathway was inhibited, and the PIK3/Akt signaling pathway was activated. Taken together, our results indicate that the photoperiod can regulate the apoptosis of the granulosa cells by regulating the concentration of FSH, activating or inhibiting the Nodal/ALK7 signaling pathway, thereby affecting the ovarian function. Our research provides an important theoretical basis for understanding the photoperiod-regulated mechanisms of the mammalian seasonal reproduction. Full article
(This article belongs to the Special Issue Advances of Endocrinology in Animal Reproduction)
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16 pages, 412 KiB  
Article
Informing Implementation: Perspectives from the Australian University Community Regarding an Animal Assisted Intervention
by Emily Cooke, Claire Henderson-Wilson, Elyse Warner and Anthony D. LaMontagne
Animals 2022, 12(24), 3569; https://doi.org/10.3390/ani12243569 - 16 Dec 2022
Viewed by 1107
Abstract
Animal Assisted Interventions (AAIs) have become increasingly popular in the university setting; however, there is limited research exploring their potential on an Australian university campus and participants’ views prior to implementation. Therefore, this study aimed to explore university staff members’ and students’ interest [...] Read more.
Animal Assisted Interventions (AAIs) have become increasingly popular in the university setting; however, there is limited research exploring their potential on an Australian university campus and participants’ views prior to implementation. Therefore, this study aimed to explore university staff members’ and students’ interest in participating in an AAI and their perspectives on intervention characteristics. This was a mixed methods study, using an online survey and semi-structured interviews. The survey had 344 responses, and 45 interviews were conducted. A large majority of participants (86%) were interested in participating in an AAI. In the survey, participants indicated their preferred intervention characteristics for the location of the intervention, frequency of participating, and ways of hearing about the intervention. Participants also expressed concerns regarding therapy animal welfare. In interviews, participants discussed various considerations which may impact the intervention (such as the accessibility of the location, the impact of workload on participating, the effectiveness of promotion strategies, and factors that may assist therapy animal welfare, such as the therapy animals’ handlers). Gaining an insight into the university community’s views prior to implementation may ensure the intervention is feasible to implement and can be beneficial to both humans and therapy animals. Full article
(This article belongs to the Section Human-Animal Interactions, Animal Behaviour and Emotion)
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8 pages, 872 KiB  
Case Report
A Case Study of Photosensitivity Associated with Anaplasma spp. Infection in Cattle
by Alina Anton and Gheorghe Solcan
Animals 2022, 12(24), 3568; https://doi.org/10.3390/ani12243568 - 16 Dec 2022
Cited by 2 | Viewed by 3373
Abstract
Anaplasma infection has not previously been reported as a cause of photosensitization. This case presents a five-year-old Holstein cow in her fifth month of gestation with skin injury of nonpigmented areas clearly delimited from adjacent unaffected pigmented skin. Specific lesions included alopecia, localized [...] Read more.
Anaplasma infection has not previously been reported as a cause of photosensitization. This case presents a five-year-old Holstein cow in her fifth month of gestation with skin injury of nonpigmented areas clearly delimited from adjacent unaffected pigmented skin. Specific lesions included alopecia, localized oedema, desquamation erythema, serous exudation, thick detached skin, fissures, crusting, and necrosis, indicating photodermatitis. Hematological abnormalities were leukocytosis with neutrophilia and normocytic hypochromic anemia. Based on a hemoparasitic examination of blood smears, the organism Anaplasma spp. was observed within the red blood cells. Biochemical analyses revealed increased serum liver enzyme activity associated with hepatocellular damage and cholestasis. This cow was RT-PCR positive for Anaplasma spp. infection. After treatment with oxytetracycline started, its condition improved within 24 h, and it recovered completely within 30 days. In this case, the underlying pathogenic mechanism of Anaplasma spp. infection facilitated the impairment of bile flow and liver function, resulting in hepatogenous photosensitization. Full article
(This article belongs to the Special Issue Advances in Ruminants Disease Prevention and Control)
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17 pages, 1241 KiB  
Article
Protein Supplementation during Mid-Gestation Alters the Amino Acid Patterns, Hepatic Metabolism, and Maternal Skeletal Muscle Turnover of Pregnant Zebu Beef Cows
by Javier Andrés Moreno Meneses, Karolina Batista Nascimento, Matheus Castilho Galvão, German Darío Ramírez-Zamudio, Tathyane Ramalho Santos Gionbelli, Marcio Machado Ladeira, Marcio de Souza Duarte, Daniel Rume Casagrande and Mateus Pies Gionbelli
Animals 2022, 12(24), 3567; https://doi.org/10.3390/ani12243567 - 16 Dec 2022
Cited by 4 | Viewed by 1514
Abstract
From 100 to 200 days of gestation, 52 cows carrying male (n = 30) or female (n = 22) fetuses were assigned to CON (basal diet—5.5% of CP, n = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 [...] Read more.
From 100 to 200 days of gestation, 52 cows carrying male (n = 30) or female (n = 22) fetuses were assigned to CON (basal diet—5.5% of CP, n = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 g/kg BW]—12% of CP, n = 26) treatments. Glucose concentrations decreased at 200 (p ≤ 0.01; CON = 46.9 and SUP = 54.7 mg/dL) and 270 days (p ≤ 0.05; CON = 48.4 and SUP = 53.3 mg/dL) for CON compared to SUP. The same pattern occurred for insulin (p ≤ 0.01). At parturition, the NEFA concentration was greater (p = 0.01, 0.10 vs. 0.08 mmol/L) for CON than for SUP. Total AA increased in SUP (p ≤ 0.03) at mid- and late-gestation compared to CON. At 200 days, CON dams carrying females had less essential AA (p = 0.01) than cows carrying males. The SUP dams had greater expressions of protein synthesis markers, namely eIf4E and GSK3β (p ≤ 0.04), at day 200 and of MuFR1 (protein degradation marker, p ≤ 0.04) at parturition. Supplemented cows had higher hepatic pyruvate carboxylase expressions (p = 0.02). Therefore, PS alleviates the restriction overload on maternal metabolism. Full article
(This article belongs to the Special Issue Improving Livestock Efficiency through Optimizing Maternal Nutrition and Management)
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12 pages, 1233 KiB  
Article
Changes in the Populations of Two Lymnaeidae and Their Infection by Fasciola hepatica and/or Calicophoron daubneyi over the Past 30 Years in Central France
by Daniel Rondelaud, Philippe Vignoles and Gilles Dreyfuss
Animals 2022, 12(24), 3566; https://doi.org/10.3390/ani12243566 - 16 Dec 2022
Cited by 1 | Viewed by 1045
Abstract
Field investigations were carried out during three periods (from 1976 to 1997, in 2013–2014, and in 2020–2021) on 39 cattle-raising farms on acidic soils to track changes in the populations of two Lymnaeidae (Galba truncatula and Omphiscola glabra) and their infection [...] Read more.
Field investigations were carried out during three periods (from 1976 to 1997, in 2013–2014, and in 2020–2021) on 39 cattle-raising farms on acidic soils to track changes in the populations of two Lymnaeidae (Galba truncatula and Omphiscola glabra) and their infection with Fasciola hepatica and/or Calicophoron daubneyi. Compared to the survey between 1976 and 1997 on these farms, there was a significant decrease in the number of the two lymnaeid populations and the size of the G. truncatula populations in both 2013–2014 and 2020–2021. This decline was significantly faster in the last nine years than it was before 2013. The area of habitats colonized by G. truncatula showed no significant variation over the years, while that of habitats with O. glabra significantly decreased in the period covered by the three surveys. The prevalence of F. hepatica infection in snails significantly decreased over the years, while C. daubneyi infection increased over time in both lymnaeid species. These changes are due to the use of triclabendazole to treat fasciolosis in ruminants since the 1990s, and are probably a consequence of the successive heatwaves that have occurred since 2018 in the region. Full article
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8 pages, 2325 KiB  
Commentary
Revisiting the Timing of Insemination at Spontaneous Estrus in Dairy Cattle
by Fernando López-Gatius
Animals 2022, 12(24), 3565; https://doi.org/10.3390/ani12243565 - 16 Dec 2022
Cited by 6 | Viewed by 2387
Abstract
Cows show spontaneous estrus over 8–20 h but become refractory to the bull about 10–12 h before ovulation. This indicates that ovulation occurs 10–12 h after the end of estrus behavior, yet spermatozoa from the bull ejaculate need to undergo maturation and capacitation [...] Read more.
Cows show spontaneous estrus over 8–20 h but become refractory to the bull about 10–12 h before ovulation. This indicates that ovulation occurs 10–12 h after the end of estrus behavior, yet spermatozoa from the bull ejaculate need to undergo maturation and capacitation for 6 to 8 h in the female reproductive tract before they are capable of fertilization. Traditionally, the onset of estrus has been considered the best timing for artificial insemination (AI) in cattle, that is, 6 to 24 h from the first signs of estrus. However, recent findings suggest this interval should be reduced to 16 to 6 h before ovulation, bringing it closer to the end of estrus. In this review, the end of estrus rather than its onset is proposed as the best guide for AI timing in dairy cattle, and physiological indicators of late estrus are discussed such as relaxation of the intravaginal part of the uterus, a lower cervical mucus viscosity and a softer pre-ovulatory follicular consistency as simple cues indicating a cow is ready for service. Full article
(This article belongs to the Special Issue Advances in Dairy Cattle Reproduction)
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12 pages, 2918 KiB  
Article
Are Tourists Facilitators of the Movement of Free-Ranging Dogs?
by Elke Schüttler and Jaime E. Jiménez
Animals 2022, 12(24), 3564; https://doi.org/10.3390/ani12243564 - 16 Dec 2022
Viewed by 1810
Abstract
Dogs are the most abundant carnivores on earth and, as such, negatively impact wildlife. Free-ranging dogs roam in many protected areas, which in turn are often tourist destinations. Whether tourists influence their roaming is largely unexplored but highly relevant to wildlife conservation. To [...] Read more.
Dogs are the most abundant carnivores on earth and, as such, negatively impact wildlife. Free-ranging dogs roam in many protected areas, which in turn are often tourist destinations. Whether tourists influence their roaming is largely unexplored but highly relevant to wildlife conservation. To address this question, we obtained (i) 81 completed questionnaires from tourists on their experience with free-ranging dogs in the remote Cape Horn Biosphere Reserve, Chile, and (ii) photographs of three camera-traps placed next to trekking trails (n = 87 trap days). A third of the participants were followed by dogs for up to four days, and 39% saw free-ranging dogs on their hikes, but neither feeding dogs nor fear of them had any influence on whether tourists were followed by dogs. Camera-traps yielded 53 independent dog sequences, recorded 32 individuals plus 14 unidentified dogs, of which only one was leashed, with a frequency of one dog every 28th person. In 17% of 53 sequences, dogs were photographed together with hikers carrying large backpacks for several-day trips. We conclude that tourists are facilitators for the movement of dogs and highlight the importance of the engagement of the tourism sector in wildlife conservation in and close to protected areas. Full article
(This article belongs to the Special Issue Ecological Impact of Feral Animals)
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