Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Bacterial Strains and Culture Conditions
2.3. C. elegans Strains, Culture Conditions, and Longevity Assays
2.4. Cell Lines, Cell Culture, and Epithelial Cell Invasion Assays
2.5. Microscopy
2.6. Statistical Analysis
3. Results
3.1. Strain-Specific E. coli Infection Impacts the Survival of C. elegans
3.2. AIEC Strains Differ in Their Cell Invasion Properties In Vitro
3.3. Assessment of the AIEC’s Pathogenicity in C. elegans
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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de Sousa Figueiredo, M.B.; Pradel, E.; George, F.; Mahieux, S.; Houcke, I.; Pottier, M.; Fradin, C.; Neut, C.; Daniel, C.; Bongiovanni, A.; et al. Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan. Microorganisms 2021, 9, 1823. https://doi.org/10.3390/microorganisms9091823
de Sousa Figueiredo MB, Pradel E, George F, Mahieux S, Houcke I, Pottier M, Fradin C, Neut C, Daniel C, Bongiovanni A, et al. Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan. Microorganisms. 2021; 9(9):1823. https://doi.org/10.3390/microorganisms9091823
Chicago/Turabian Stylede Sousa Figueiredo, Maria Beatriz, Elizabeth Pradel, Fanny George, Séverine Mahieux, Isabelle Houcke, Muriel Pottier, Chantal Fradin, Christel Neut, Catherine Daniel, Antonino Bongiovanni, and et al. 2021. "Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans’ Lifespan" Microorganisms 9, no. 9: 1823. https://doi.org/10.3390/microorganisms9091823