Seasonal Effect on Bacterial Communities Associated with the Rhizospheres of Polhillia, Wiborgia and Wiborgiella Species in the Cape Fynbos, South Africa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description and Soil Sampling
2.2. Total Bacterial Genomic DNA Extraction from Rhizosphere Soils
2.3. Library Preparation
2.4. Sequencing and Data Assembling
3. Results
3.1. Site Elevation and Physical Properties of Rhizosphere Soils
3.2. Chemical Properties of Rhizosphere Soils
3.3. Quantity and Quality of Raw Sequences Generated
3.4. Rhizosphere Bacterial Communities
3.4.1. Polhillia Brevicalyx
3.4.2. Polhillia Pallens
3.4.3. Wiborgia Sericea
3.4.4. Wiborgia Obcordata
3.4.5. Wiborgiella Sessilifolia
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Collecting Site | Geographic Co-Ordinates | District |
---|---|---|---|
Polhillia brevicalyx | Ghwarriekop farm | 34°33′53″S 19°59′43″E | Overberg |
Polhillia pallens | Witkoppies farm | 34°33′53″S 19°59′43″E | Overberg |
Wiborgia obcordata | Bushmans Kloof | 32°07′14″S 19°06′28″E | Cederberg |
Wiborgia sericea | Traveller’s Rest farm | 32°04′15″S 19°04′32″E | Cederberg |
Wiborgiella sessilifolia | Bredasdorp/Elim Pass | 34°37′58″S 19°49′399″E | Overberg |
Dry Season | Wet Season | |||
---|---|---|---|---|
Plant Species | Soil Type | Elevation (m) | θm Water Content (%) | |
Polhillia brevicalyx | Clay | 310 | 8.21 ± 0.00 | 25.47 ± 0.31 |
Polhillia pallens | Clay | 152 | 9.26 ± 0.01 | 26.55 ± 0.06 |
Wiborgia sericea | Sandy | 120 | 6.37 ± 0.01 | 20.00 ± 0.00 |
Wiborgia obcordata | Sandy | 441 | 3.93 ± 0.05 | 11.90 ± 0.03 |
Wiborgiella sessilifolia | Loam | 311 | 5.42 ± 0.03 |
pH and Minerals (mg/kg) | Polhillia brevicalyx | Polhilliapallens | Wiborgiella sessilifolia | Wiborgia obcordata | Wiborgiasericea |
---|---|---|---|---|---|
Concentrations | |||||
pH(H2O) | 5.53 ± 0.03 | 4.45 ± 0.05 | 8.20 ± 0.00 | 4.33 ± 0.09 | 4.47 ± 0.09 |
Magnesium | 4.55 ± 0.02 | 2.67 ± 0.01 | 3.08 ± 0.01 | 0.25 ± 0.01 | 0.27 ± 0.01 |
Potassium | 170.18 ± 4.83 | 234.00 ± 1.00 | 53.67 ± 1.76 | 27.33 ± 1.76 | 37.00 ± 0.58 |
Sodium | 110.22 ± 3.42 | 130.00 ± 0.00 | 64.33 ± 0.88 | 4.33 ± 0.33 | 9.00 ± 0.00 |
Phosphorus | 22.00 ± 0.00 | 43.50 ± 0.50 | 15.67 ± 0.33 | 6.00 ± 0.58 | 8.33 ± 0.33 |
Copper | 0.84 ± 0.00 | 0.46 ± 0.02 | 0.12 ± 0.01 | 0,14 ± 0.00 | 0.14 ± 0.1 |
Zinc | 7.28 ± 0.05 | 2.84 ± 0.17 | 0.41 ± 0.01 | 0.18 ± 0.01 | 0.66 ± 0.03 |
Boron | 10.67 ± 8.92 | 0.61 ± 0.01 | 0.80 ± 0.05 | 0.07 ± 0.00 | 0.12 ± 0.1 |
Carbon (%) | 4.22 ± 0.13 | 7.76 ± 0.04 | 2.82 ± 0.15 | 0.39 ± 0.02 | 0.38 ± 0.02 |
Iron | 105.85 ± 0.45 | 174.60 ± 6.20 | 46.98 ± 0.05 | 66.19 ± 2.88 | 129.2 ± 5.45 |
NH4+ (%) | 0.40 ± 0.01 | 0.60 ± 0.00 | 0.67 ± 0.48 | 0.04 ± 0.01 | 0.03 ± 0.00 |
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Mpai, T.; Jaiswal, S.K.; Cupido, C.N.; Dakora, F.D. Seasonal Effect on Bacterial Communities Associated with the Rhizospheres of Polhillia, Wiborgia and Wiborgiella Species in the Cape Fynbos, South Africa. Microorganisms 2022, 10, 1992. https://doi.org/10.3390/microorganisms10101992
Mpai T, Jaiswal SK, Cupido CN, Dakora FD. Seasonal Effect on Bacterial Communities Associated with the Rhizospheres of Polhillia, Wiborgia and Wiborgiella Species in the Cape Fynbos, South Africa. Microorganisms. 2022; 10(10):1992. https://doi.org/10.3390/microorganisms10101992
Chicago/Turabian StyleMpai, Tiisetso, Sanjay K. Jaiswal, Christopher N. Cupido, and Felix D. Dakora. 2022. "Seasonal Effect on Bacterial Communities Associated with the Rhizospheres of Polhillia, Wiborgia and Wiborgiella Species in the Cape Fynbos, South Africa" Microorganisms 10, no. 10: 1992. https://doi.org/10.3390/microorganisms10101992