The phytoecdysteroid 20-hydroxyecdysone (20E) is widely used for resistance training (RT). Little is known about its potential ergogenic value and detraining effects post-RT. This study aimed to examine the effects of 20E extracted from Asparagus officinalis
) on muscle strength and mass, as well as anabolic and catabolic hormones following RT and detraining. Twenty males, aged 20.1 ± 1.1 years, were matched and randomly assigned to consume double-blind supplements containing either a placebo (PLA) or 30 mg/day of 20E for 12 weeks of RT and detraining. Before and after RT and detraining, muscle strength and mass and anabolic and catabolic hormones were measured. This study found that 20E reduced cortisol levels significantly (p
< 0.05) compared to the PLA, yet no effect was observed on muscle mass, strength, or anabolic hormones after RT. Subsequent to 6 weeks of detraining, the 20E demonstrated a lower percentage change in 1RM bench press/FFM than the PLA (p
< 0.05). Compared to the PLA, detraining throughout the 12 weeks resulted in a lower percentage change in thigh (p
< 0.05) and chest (p
< 0.01) circumferences, as well as reduced cortisol levels (p
< 0.01), with 20E. Our findings demonstrate that 20E supplementation is a promising way to maintain muscle mass and strength during detraining. Accordingly, 20E may prevent muscle mass and strength loss due to detraining by lowering catabolic hormone levels.