Next Article in Journal
Mechanics of the Prey Capture Technique of the South African Grassland Bolas Spider, Cladomelea akermani
Previous Article in Journal
Control of Aculops lycopersici with the Predatory Mite Transeius montdorensis
Previous Article in Special Issue
Fireflies in Art: Emphasis on Japanese Woodblock Prints from the Edo, Meiji, and Taishō Periods
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Insects
Volume 13
Issue 12
10.3390/insects13121117
insects-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Unraveling the Role of Lac Insects in Providing Natural Industrial Products

by
Nawaz Haider Bashir
1,†,
Huanhuan Chen
1,2,†,
Shahzad Munir
3,
Weiwei Wang
4,
Hang Chen
4,
Yong-Kang Sima
5,* and
Jiandong An
2,*
1
College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China
2
Key Laboratory of Insect-Pollinator Biology of Ministry of Agriculture and Rural Affairs, Institute of Agricultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China
3
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China
4
Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, China
5
Yunnan Academy of Forestry and Grassland Science, Kunming 650201, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Insects 2022, 13(12), 1117; https://doi.org/10.3390/insects13121117
Submission received: 16 November 2022 / Revised: 30 November 2022 / Accepted: 30 November 2022 / Published: 5 December 2022
(This article belongs to the Special Issue Insects and Art)
Browse Figures
Versions Notes

Abstract

:

Simple Summary

Since ancient times, people have used insects, such as scale insects (lac dye, resin, and wax), honey bees (honey and wax), and silkworms (silk), to make natural products in many ways. Lac is the only natural raw material derived from insects, being obtained from several species of the genus Kerria. Lac is commercially cultivated in southeastern Asian countries, including China (Kerria yunnanensis), India (K. lacca), Thailand (K. chinensis), and Myanmar (K. nepalensis). It is non-toxic, insulating, moisture-resistant, adhesive, tasteless, and has a smooth texture. Lac has been historically employed to finish a small number of products, but it is currently used in various fields. In this review, we mainly focus on the importance of lac genes that are responsible for lac secretion. In addition, information gaps, potential future research directions, and areas that require further research are highlighted.

Abstract

In the current era, products made from organic materials enjoy a privileged position because of their inherent safety. The eco-friendly properties of natural lac resins have increased their demand in many industries. It is secreted by sucking insects (Hemiptera, Kerriidae) and comprises three major components, viz., resin, dye, and wax. Lac insects are generally bivoltine in nature and are distributed in tropical and sub-tropical regions with complex multi-trophic habitats. Because of their sedentary habits, lac insects are more vulnerable to predators, parasitoids, squirrels, and rats, leading to a more than 50% reduction in production yield. To increase lac production, advanced-level molecular research is required to figure out the mechanism behind lac synthesis and secretion to improve lac yield and quality. The present review highlights metamorphosis, sexual dimorphism, multi-trophic habitat, host plants, and natural enemies of lac insects, lac composition, and applications, emphasizing the role of microbes, potential lac genes, and lac synthesis mechanisms in enhancing lac quality and production. The information provided here might be useful for lac researchers and for stakeholders aiming to make their products more eco-friendly.

1. Introduction

Lac is a combination of natural resins produced by scale insects [1]. At first, the use of lac was limited to applications such as in decorating wood and as an adhesive for ceramics [2]. It is currently used in a wide range of applications, such as coating pharmaceutical pills, food, musical instruments, material conservation, painting, and electronics insulation, as well as in the military industry [3,4]. Lac has insulation properties, adhesiveness, tastelessness, moisture resistance, and a smooth film texture, and is non-toxic [5,6,7]. Due to these properties, lac is anticipated to be widely used in the future for finishing many industrial products [1,8,9]. Lac production is limited to south, east, and southeast Asian countries such as China, India, Pakistan, Bangladesh, Indonesia, Vietnam, Laos, and Myanmar [10,11].
Lac insects are members of the family Kerriidae (Hemiptera), also known as scale insects, which produce a resinous material that forms a hard scale test over their bodies [12,13]. This family is described based on the sclerotized characteristics of an adult female [14]. These are sucking insects that live and feed on various plants [15]. These insects prefer warm climates and are distributed in tropical and sub-tropical regions [10]. Lac insects have sexual dimorphism; females and males have different life cycles and morphology (Table 1) [4]. The male is holometabolous, while the female shows paurometabolous metamorphosis. The adult male is mobile and has legs, well-developed antennae, simple eyes, and no mouthparts [16]. The adult female is immobile and bound in resinous secretion, with a sac-like body appearance, vestigial antennae, and no body segmentation, legs, and eyes [17]. Generally, lac insects are bivoltine, having winter and summer generations every year, from July to December and January to June, respectively (Figure 1) [18]. Female larvae and males can produce a small amount of lac. The female can produce and secrete reasonable amounts of lac at the adult stage [2].
The family Kerriidae comprises 9 known genera and 101 species [13,15]. The members of the genus Kerria can produce true lac [18]. Presently, the genus Kerria contains 29 species worldwide [13], among which the highest number of species is distributed in India (24), followed by China (11) and Thailand (7), with a few species also being reported in Pakistan, Myanmar, and Vietnam [15]. Some species in this genus, such as Kerria chinensis, K. yunnanesis, K. lacca, K. ruralis, K. sindica, K. nepalensis, and K. pusana, can produce industrial lac [4].
Lac insects would not exist without the support of host plants [20], and up to now, more than 400 lac host plants have been recorded [21]. These insects fulfill their nourishment by sucking the phloem sap of host plants [22]. Lac insects are very particular in their selection of host trees. They remain attached to the host tree throughout their life, except the crawler and male adult [10].
Polyterpene esters and hydroxyl fatty acids are both parts of lactones and lactides, which are major components of lac resin [5]. The biochemical pathways and molecular mechanisms for lac production and secretion are not yet clear. However, Wang et al. [4] identified 28 candidate genes involved in lac production and secretion, including genes involved in fatty acid synthesis (which may produce hydroxyl fatty acids), terpenoid biosynthesis (which may be involved in the production of sesquiterpene acids), and UDP-driving glycosylation (which indirectly or directly takes part in the accumulation of activated sugars). Another study identified more than ten genes that may be involved in the biosynthesis pathway of the lac dye [23].
Based on overall studies reported about lac insects and their industrial use, the present review is intended to highlight the available studies on the trophic levels, host plants, natural enemies, molecular investigations, and native microbial diversity of lac, which could open new doors for future advancements in lac production. We focus on highlighting the lac genes that are responsible for lac secretion and production. Moreover, knowledge gaps, future research directions, and areas that need in-depth research are underlined.

2. Lac Insect Interaction

The habitat of the lac insect is intricate, with a multi-trophic web of fauna and flora. It includes a rich biodiversity of lac host plants, pests of host plants, lac insects, lac insect predators, microbes, harmful parasites, and beneficial parasites [21]. Lac insects are associated with more than 30 primary parasitoids, 20 lac predators, and 40 secondary parasitoids [24]. Lac host plants represent the first tropic level, while lac insects and various pests of host plants comprise the second tropic level. Predators with primary parasitoids constitute the third tropic level, and secondary parasitoids (parasitoids of lac insect predators) make up the fourth tropic level (Figure 2) [25].

3. Lac Composition and Their Industrial Application

Lac is an insect-secreted resinous substance of animal origin [28]. The composition of lac depends on the insect’s species, environmental conditions, and host tree [4]. Generally, lac has three main components, viz., dyes, resins, and shellac wax [29]. Lac resins are mixtures of lactides and lactones, which are formed from polyterpene esters and hydroxyl fatty acids (Figure 3) [5,7]. Lac dye is composed of an anthraquinone derivative, while shellac wax is a mixture of higher alcohol esters, hydrocarbons, and acids. In addition, it has some other chemicals that are used for industrial purposes. Nowadays, lac is considered more valuable as it is biodegradable and provides an opportunity for landless farmers to earn money [30].
Lac resins are used in the following industries: the food industry (food-grade ink, fruit coating, non-toxic food packaging printing, chocolates, aluminum foils, and coffee beans); the pharmaceutical industry (coating of tablets and medicines, microencapsulation of vitamins, and marking ink for capsules and tablets); the cosmetic industry (microencapsulated perfumes, bangles, hair spray, lacquer, eye shadows, mascara, lipstick, and nail polish); the electrical industry (green electronics, coating of isolators, PCBs and spark plugs, coating of electric motors and transformers, electrical lamps, transistors, shellac bond powder, air-drying, baking-type, and anti-tracking insulating varnishes); the leather industry (topdressing material, dying and lacquering, leather shoe polishing cream, adhering and coating of leather with plastic and metallic foils); the adhesive industry (optical and gasket cement, sealing wax, fixing steam and water pipes, adhesives for chips and solar cells, and hot melt and polymer adhesives); the varnish, printing, and agriculture industries (floor and furniture polish, sealers, paints for wood, water-soluble lac for earthenware, agent for printing ink (flexible and fast-drying), metal and dry lacquering of wood, heat and waterproof varnish, spray-able varnish, lac-coated urea, and weedicide, nematicidal activity, synthesis of sex pheromones and PGRS, for mosquito control, slow-release fertilizer, and pesticide); and miscellaneous uses (polishing stone, protective lacquer for silvering mirrors, preparation of buttons and toys, high-efficiency igniter-cum-fuel, jewelry, diamond and crystal cutting, proofing, and stiffening in hat making) (Figure 4A) [1,9,31].
In addition to these, byproducts of lac wax and dye are widely used. Lac wax is used in fruit coating, bottle sealer, chalk, electrical potting compounds, lipstick, shoe automobiles, and floor polishes. Lac dye is used in pill coatings, food coatings, soft drink formulations, confectionery, chocolate coating, and the dyeing of wool and silk [32]. In recent years, most lac-related patents have been registered in the food industry, followed by the pharmaceutical, electrical, adhesive, varnish, and ink industries [9,33,34,35,36,37,38,39,40,41,42,43] (Figure 4B).

4. Important Lac Genes Involved in Production

Lac resins are mixtures of lactides and lactones, which are formed from polyterpene esters and hydroxyl fatty acids [5]. In the cytoplasm, polyterpene esters are formed through the MVA (mevalonate pathway) [44,45], in which acetyl-CoA is transformed into mevalonic acids by hydroxymethylglutaryl-CoA synthase and reductase. Finally, mevalonic acids are converted to sesquiterpene acids by terpene synthases (TPSs), and sesquiterpene acids are converted into sesquiterpene acid esters [46]. The fatty acid synthesis pathway is responsible for the production of hydroxyl fatty acids by fatty acid synthetases (FASs), elongases of very-long-chain fatty acids (ELO), and fatty acid desaturases (FAD) (Figure 3).
Wang et al. [4] identified 28 candidate genes based on the correlation between gene expression levels and lac accumulation, of which 19 genes are involved in fatty acid synthesis, 4 genes are related to terpenoid biosynthesis, and 5 genes are found in UDP-driving glycosylation. By comparing the expression levels of lac synthesis active and non-active stages in Kerria lacca, it was revealed that two genes, acyl-CoA delta desaturase and decaprenyl diphosphate synthase, have an important role in the biosynthesis of lac resin [47]. Shamim et al. [23] selected a few genes that may be involved in the biosynthesis of lac dye, including acyltransferase, enoyl reductase, PKS enzyme partial sequences, 3-oxoacyl-(ACP) synthase II, acetyl CoA acetyl transferase, oxido-reductase, [ACP] S-malonyl transferase, methyl transferase, and oxido-reductase, β subunit. According to Shamim et al. [48], there are two genes (prenyltransferase and cytochrome P450) involved in the multi-step lac resin production pathway.
Bashir and co-workers [19] studied variation in molecular markers during lac secretion minimum and maximum stages of K. lacca and found 47 candidate lac-related genes, of which 30 were associated with 114 molecular markers (40 InDels and 74 SNPs). Among these genes, 20 are associated with both InDels and SNPs, with 7 and 13 variants being specifically related to InDels and SNPs, respectively. Finally, based on molecular markers, 23 candidate genes were found to be potentially involved in lac production and secretion, including terpenoid backbone biosynthesis, fatty acid biosynthesis, and fatty acid elongation and metabolism.
Recent research on the genes involved in lac production and secretion found that one gene (decaprenyl-diphosphate synthase) is common in all three studies (ABC), one gene (acyl-CoA delta desaturase) is common in A and B, and three genes (acyl-CoA dehydrogenase, fatty acid synthase, and acyl-CoA synthetase) are common in A and C (Figure 5). Decaprenyl diphosphate synthase is a long-chain polyprenyl diphosphate synthase of the family of prenyltransferases that has important physiological roles in organisms [49,50,51]. Aphids possess decaprenyl diphosphate synthase, a type of isopentenyl diphosphate synthase [52]. Isopentenyl diphosphate synthases convert these precursors to farnesyl diphosphate, and through terpene synthases, farnesyl diphosphates are converted to sesquiterpene [47,53]. Acyl-CoA desaturase, the second most common gene found in both investigations (A and B), is present in animals, bacteria, and fungi [54]. Insect acyl-CoA desaturase has been extensively researched in Drosophila melanogaster [55], Tribolium castaneum [56], Acheta domesticus [57], Adelphocoris suturalis [58], and Nilaparvata lugens [59] and has been shown to be important in fatty acid biosynthesis [60], cold tolerance [61], eating behavior [62], perception of semiochemical sensing [63], and larval development [64].

5. Deciphering the Transcriptomic of Lac Secretion Mechanism

Transcriptomic studies have provided the knowledge needed to understand genome expression [65] and help us recognize the structure of genes, gene regulation, gene expression, and genome dynamics in an organism [66]. Many studies have dealt with transcriptomic analysis and revealed various mechanisms, such as induced defense responses against whitefly (Bemisia tabaci) and aphid (Aphis gossypii) [67], embryogenesis in brown planthopper (Nilaparvata lugens) [68], mechanisms involved in the maternal effect on egg diapause against locusts (Locusta migratoria) [69] and silkworm (Bombyx mori) [70], mechanisms of stress responses against cutworm (Spodoptera litura) [71], and mechanisms of heat tolerance in snout moth (Glyphodes pyloalis) [72].
Previous studies showed that fatty acid synthesis, terpenoid biosynthesis, UDP-driving glycosylation, and polyketide pathways are involved in the synthesis of shellac. An annotation of Kerria chinensis elucidated almost 150 genes found in terpenoid backbone biosynthesis, about 15 of which are involved in sesquiterpenoid biosynthesis, and more than 700 genes are known to be fatty-acid-related pathways [4]. Several studies have also provided supposed pathways and candidate genes that may be involved in the synthesis of lac resin and lac dye [23,48]. Nevertheless, the evidence-based molecular mechanisms and metabolic pathways of lac synthesis are still unknown in lac insects. Omics investigations are required to elucidate the mechanisms and pathways by comparison between lac secretion active (early, mid, and late female adult) and non-active (larval and adult male) life stages of lac insects (Figure 6).

6. Role of Native Microbial Diversity of Lac Insects

The native microbial diversity of any host plant plays a significant role in its interaction with the environment and helps in its resistance against invading enemies in the form of other insects or pathogens. We suggested above that most of the lac genes and expression of lac genes in insects are important for production of lac; however, the role of microbes present in lac insects should not be denied a role in lac production. The roles of many microbes have not yet been explored, but these beneficial microbes have hidden arsenals that could be studied in more detail in future research. Bacterial study revealed that early stages of Kerria lacca contained Mucilaginibacter and Wolbachia, whereas adult female contributed genera Pantoea and Wolbachia [73]. Other studies also reported a few bacterial genera from lac insects, such as Bacillus, Micrococcus, Wolbachia, Arthrobacter, Curtobacterium, and Clostridium [74,75,76]. Yeast-like fungal symbionts are also present in lac insects [77].
Many types of symbiosis have been identified in different insect orders, such as Hemiptera, Diptera, Lepidoptera, and Isoptera. Insects harbor microbes in the digestive tract, on the integuments, and in some unique structures within their bodies [78,79]. The roles of microbes in insects include protection, resistance, digestion, nutrition, production, and communication [73,80]. Symbiosis with bacterial communities is obligatory in insects [81] whose diet is imbalanced, such as sap-sucking insects (phloem sap), termites (wood), and mosquitoes (blood feeder) [77]. Since lac insects are sap feeders [82], it is observed that indigenous microbiota must contribute to the provision of nutrients (amino acids and vitamins that are not obtained from phloem) and other physiological activities [73,75]. As mentioned earlier, a few bacteria that may be beneficial for lac production have been reported in lac insects [77]. However, the significance of the presence of these candidate isolates is still unknown [75].

7. Important Host Plants for Lac

Lac insects depend on plants as hosts for their food requirements. Firstly, 4 species are known as host plants of lac insects [83]; recently, more than 400 plant species have been reported worldwide [11,84]. These plants are divided into three types, common, occasional, and rare, as per their suitability and distribution [85]. Occasional and rare host plants do not sustain lac crops; so, these plants are important for preserving some rare lac insect species only [27]. Three species of common host plants, Schleichera oleosa (nagoli or kusumr) (Figure 7), Butea monosperma (dhak or palas), and Ziziphus mauritiana (ber), are considered important for lac production. The quick-growing lac hosts are Cajanus cajan (Figure 7), Flemingia semialata, F. macrophylla, Acacia nilotica, A. catechu, A. auriculiformis, and Ziziphus mauritiana, and they are capable of sustaining a lac crop over one generation (Table 2) [27].
Indian wild ber serves as a host plant for lac insects in different agro-climatic zones of Punjab, India [84]. In India, peepal (Ficus religiosa), ber (Ziziphus mauritiana), litchi (Litchi chinensis), kikar (Acacia nilotica), and sirin (Albizia spp.) are known as major host plants of lac insects [84,88,89], whereas Acacia nilotica, A. catechu, Samanea saman, Butea monosperma, and Ziziphus mauritiana are common lac host plants in Bangladesh [90]. The various lac host plants in China are Dalbergia assamica, D. szemaoensis, D. obtusifolia, Ficus altissima, F. racemosa, and Pueraria tonkinensis [3,91].
Seven species, including K. chinensis, K. fici, K. lacca, K. pusana, K. maduraiensis, K. destructor, and K. canalis, are known to feed on the rain tree host plant Samanea saman with slight to severe levels of lac encrustation [13]. Nevertheless, K. destructor has been reported to kill large rain trees [28] via repeated generations, aggressive multiplication, and improper management (pruning, watering, fertilizing, weed prevention, disease control, and pest management). Other lac insects form severe encrustation on the branches and twigs without showing any symptoms of dieback. These species might cause chronic damage to the host plant if it is not properly managed for a long time.

8. Natural Enemies of Lac Insects and Their Management

Lac crop losses are higher than agricultural crop losses [92]. Lac pests destroy around two-thirds of the feasible lac crop, leaving behind only one-third for the cultivation to harvest. Lac insects live on host plants; being of a sedentary nature, they are more susceptible to being attacked by a number of predators and parasitoids [27,93]. Primary (35 species) and secondary (45 species) parasitoids have been reported to belong to the superfamily Chalcidoidea [24]. Among these, more harmful parasitoids of lac insects are Aprostocetus (Tetrastichus) purpureus (Cameron), Tachardiaephagus tachardiae (Howard), and Parechthrodryinus clavicornis (Cameron) [94]. However, 20 predators of lac insects have been recorded, including the major predators Eublemma amabilis Moore (white moth), Pseudohypatopa pulverea Meyr (black moth), and Chrysopa sp. (green lace wing) [1,16], which are responsible for 35–40% of lac crop losses as reported by earlier work [94,95]. In the winter season, the sporadic pests Chrysopa lacciperda and C. madestes are most prevalent and cause significant mortality in lac insects [20,92]. During the rainy season, Sharma, et al. [96] observed 26% and 18% parasitization in rangeeni and kusmi strains, respectively. They observed up to nine and six parasitoids in a single cell of rangeeni and kusmi strains, respectively. Both squirrels and rats can cause serious damage, decreasing the amount of brood sticks to up to 50%. Fungi and bacteria cause damage to the lac crop by affecting lac insects at different stages [94].
The best way to protect crops from pests is to follow preventive measures such as (a) selecting mature, healthy, and brood lac free of parasitoids and predators for crop inoculation (b) after 2–3 weeks of inoculation, removing sticks with no brood lac [94] (c) scrapping excessive stick lac, and (d) processing as early as possible after harvesting the crop [27]. Early inoculation (10–15 days), trap cropping, and intercropping (okra and cotton) techniques can be used as cultural control. In mechanical control, synthetic net container bags (sixty mesh) may be used for inoculation of brood lac [20]. Nymphs will crawl out from minute pores and settle on the twigs of plants, while emerging adult predator moths became entrapped within the net. Effective control of Pseudohypatopa pulverea and Eublemma amabilis can be achieved by the use of the biopesticide Bacillus thuringiensis Berliner in the field. Two ant predators (Solenopsis geminata and Camponotus compressus) have been evaluated for control of lac predators via attacking the pupae and larvae of P. pulverea and E. amabilis. Several egg parasitoids have been used for the management of lac predators such as Telenomus remus, Trichogrammatoidea bactrae, Trichogramma brasiliensis, T. pretiosum, and T. chilonis. Pests can be controlled by applying systemic insecticides that are safe for lac nymphs and effective against predators. A combination of the above components may reduce the pest population and increase lac yield [16,20,27].

9. Conclusions and Future Research Directions

In view of the biosafety of natural products, this review highlights detailed information, including the life stages, distribution, and interaction of lac insects; their applications and composition and the lac genes involved in lac synthesis; and microbial diversity, host plants, and natural enemies of lac insects. The factors contributing to yield reduction during lac crop cultivation are highlighted, as well as how they might be improved. Lac insects are attacked by vertebrate and invertebrate pests, resulting in yield losses of up to 50%. This loss can be reduced through timely and effective management. Earlier investigations identified a few genes that may be responsible for lac production, and at least four to five common genes that need to be verified through gene knockout or gene silencing techniques. Until now, there has been no information about indigenous associations involved in lac production. Further investigations are required to determine the role of native microbes in the female lac insect, as this is one of the most important factors in lac yield because commercial lac is obtained solely from female lac insects. In addition, the response of native microbes already present in lac insects and the lac resins on trees during the introduction of identified candidate microbes from different parts of lac insects could be investigated. Advanced omics research (transcriptomics, genomics, metabolomics, and proteomics) could help us to identify the mechanisms and pathways of lac synthesis, which would enhance lac yield.

Author Contributions

Conceptualization, N.H.B. and S.M.; methodology, H.C. (Huanhuan Chen), W.W. and H.C. (Hang Chen); software, S.M. and W.W.; validation, N.H.B. and S.M.; formal analysis, H.C. (Huanhuan Chen) and H.C. (Hang Chen); investigation, W.W. and J.A.; resources, W.W. and H.C. (Hang Chen); writing—original draft preparation, N.H.B.; writing—review and editing, N.H.B. and S.M.; visualization, H.C. (Hang Chen); supervision, H.C. (Huanhuan Chen), Y.-K.S. and J.A.; project administration, Y.-K.S. and J.A.; funding acquisition, H.C. (Huanhuan Chen), Y.-K.S. and J.A. All authors listed have made a substantial, direct, and intellectual contribution to the work. All authors contributed to the article and approved the submitted version. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by The Identification of International Science and Technology Commissioners (Yong-Kang Sima) of Yunnan Province in 2022 (project no. 202203AK140005), National Natural Science Foundation of China (grant no. 31860620), Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2015-IAR), and the National College Students Innovation and Entrepreneurship (project no. 202110684026, 202110684052).

Data Availability Statement

All data presented in this study are available in the article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Mohanasundaram, A.; Kaprakkaden, A.; Nebapure, S.M.; Sharma, K.K.; Naaz, N.; Mishra, R.; Shree, U. Electroantennography and behavioral studies of Eublemma amabilis [Moore] and Pseudohypatopa pulverea [Meyr] in relation to volatiles of lac insect (Kerria lacca Kerr.) and its associated products. Int. J. Trop. Insect Sci. 2022, 42, 2313–2324. [Google Scholar] [CrossRef]
  2. Chen, X.M.; Chen, Y.Q.; Zhang, H.; Shi, L. Lac Insects Breeding and Lac Processing; Chinese Forestry Press: Beijing, China, 2008. [Google Scholar]
  3. Chen, X.M.; Chen, H.; Ying, F.; Rui, H.; Yang, Z.X. Status of two species of lac insects in the genus Kerria from China based on morphological, cellular, and molecular evidence. J. Insect Sci. 2011, 11, 106. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Wang, W.W.; Liu, P.F.; Lu, Q.; Ling, X.F.; Zhang, J.W.; Chen, M.S.; Chen, H.; Chen, X.M. Potential Pathways and Genes Involved in Lac Synthesis and Secretion in Kerria chinensis (Hemiptera: Kerriidae) Based on Transcriptomic Analyses. Insects 2019, 10, 430. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Chen, X.M. Biodiversity of Lac Insects; Yunnan Science and Technology Press: Kunming, China, 2005; p. 96. [Google Scholar]
  6. Chen, Y.Q.; Li, Q.; Chen, Y.L.; Lu, Z.X.; Zhou, X.Y. Ant diversity and bio-indicators in land management of lac insect agroecosystem in Southwestern China. Biodivers. Conserv. 2011, 20, 3017–3038. [Google Scholar] [CrossRef]
  7. Wang, W.W.; Ling, X.F.; Bashir, N.H.; Lu, Q.; Zhang, J.W.; Li, T.C.; Chen, H. Selection and evaluation of reference genes for quantitative real-time PCR analysis in lac insect (Kerria lacca). Entomol. Res. 2022, 52, 57–67. [Google Scholar] [CrossRef]
  8. Chen, X.M.; Feng, Y. Chapter 1—Lac insects. In An Introduction to Resource Entomology; Science Press: Beijing, China, 2009; pp. 12–28. [Google Scholar]
  9. Thombare, N.; Kumar, S.; Kumari, U.; Sakare, P.; Yogi, R.K.; Prasad, N.; Sharma, K.K. Shellac as a multifunctional biopolymer: A review on properties, applications and future potential. Int. J. Biol. Macromol. 2022, 215, 203–223. [Google Scholar] [CrossRef]
  10. Sharma, K.K. Understanding the Diversity of Lac Insects of Kerria spp. in India and the Nature of Insect-Host Plant Interaction. In Natural Resource Management: Ecological Perspectives; Peshin, R., Dhawan, A.K., Eds.; Springer Nature: Cham, Switzerland, 2018; pp. 219–233. [Google Scholar] [CrossRef]
  11. Borah, N.; Garkoti, S.C. Indigenous lac culture and local livelihood: A case study of Karbi community of Assam, North-Eastern India. Indian J. Tradit. Knowl. 2020, 19, 197–207. [Google Scholar]
  12. Gullan, P.J.; Kondo, T. The morphology of lac insects (Hemiptera: Coccoidea: Kerriidae). In Proceedings of the XI International Symposium of Scale Insect Studies, Oeiras, Portugal, 24–27 September 2007; pp. 63–70. [Google Scholar]
  13. Rajgopal, N.N.; Mohanasundaram, A.; Sharma, K.K. A new species of lac insect in the genus Kerria Targioni Tozzetti (Hemiptera: Coccomorpha: Tachardiidae) on Samanea saman (Fabaceae) from India. Zootaxa 2021, 4938, 60–68. [Google Scholar] [CrossRef]
  14. Rajgopal, N.N. Taxonomic aspects of Lac Insect. In Training Manual: ICAR-NAHEP-CAAST Short Training on Natural Resins and Gums: Production, Processing, Value Addition and Marketing; Prasad, N., Thombare, N., Singh, R., Sakare, P., Sharma, K.K., Eds.; ICAR-IINRG: Ranchi, India, 2020; pp. 34–39. [Google Scholar]
  15. Varshney, R.K.; Sharma, K.K. Lac Insects of the World—An Updated Catalogue and Bibliography; Indian Council of Agricultural Research, Indian Institute of Natural Resins and Gums: Ranchi, India, 2020; p. 84. [Google Scholar]
  16. Mohanasundaram, A.; Sharma, K.K.; Naaz, N. Lac insect, natural enemies and their management. In Commercial Entomology; Kumar, A., Kumar, N., Chand, H., Eds.; New India Publishing Agency: New Delhi, India, 2019; pp. 111–128. [Google Scholar]
  17. Mishra, Y.D.; Yadav, S.K.; Singh, R.K. Lac host plants. In Beneficial Insect Farming-Benefits Livelihood Generation; Sharma, K.K., Monobrullah, M., Mohanasundaram, A., Ramani, R., Eds.; ICAR-Indian Institute of Natural Resins & Gums: Ranchi, India, 2016; pp. 66–78. [Google Scholar]
  18. Ramani, R.; Sharma, K.K. Lac insects. In Beneficial Insects Farming; Sharma, K.K., Monobrullah, M., Mohanasundaram, A., Ramani, R., Eds.; ICAR-Indian Institute of Natural Resins and Gums: Ranchi, India, 2016; pp. 53–65. [Google Scholar]
  19. Bashir, N.H.; Wang, W.W.; Ling, X.F.; Zhang, J.W.; Lu, Q.; He, R.; Chen, H. Characterization of Potential Molecular Markers in Lac Insect Kerria lacca (Kerr) Responsible for Lac Production. Insects 2022, 13, 545. [Google Scholar] [CrossRef]
  20. Shah, T.H.; Thomas, M.; Bhandari, R. Lac production, constraints and management: A review. Int. J. Curr. Res. 2015, 7, 13652–13659. [Google Scholar]
  21. Sharma, K.K. Lac insects and host plants. In Industrial Entomology; Omkar, Ed.; Springer Nature: Singapore, 2017; pp. 157–180. [Google Scholar]
  22. Sharma, K.K.; Jaiswal, A.K.; Kumar, K.K. Role of lac culture in biodiversity conservation: Issues at stake and conservation strategy. Curr. Sci. 2006, 91, 894–898. [Google Scholar]
  23. Shamim, G.; Pandey, D.M.; Sharma, K.K.; Ramani, R. Genes Involved in the Biosynthesis of Lac Dye Constituents in Indian Lac Insect, Kerria lacca (Kerr). J. Entomol. Res. Soc. 2016, 18, 7–17. [Google Scholar]
  24. Meena, S.C.; Sharma, K.K. Species composition and richness of entomo-fauna associated with lac insect, Kerria lacca Kerr in western plains of India. J. Entomol. Zool. Stud. 2018, 6, 684–690. [Google Scholar]
  25. Singh, J.P. Effect of broodlac treatment with insecticides on population reduction of predators and parasitoids of lac insect, Kerria lacca (Kerr). Natl. Acad. Sci. Lett. 2013, 36, 379–383. [Google Scholar] [CrossRef]
  26. Bashir, N.H.; Wang, W.W.; Liu, J.; Wang, W.; Chen, H. First record of the lac-producing species Kerria nepalensis Varshney (Hemiptera, Kerriidae) from China, with a key to Chinese species. ZooKeys 2021, 1061, 1–9. [Google Scholar] [CrossRef]
  27. Mishra, Y.D.; Kumar, P. Lac culture. In Industrial Entomology; Omkar, Ed.; Springer Nature: Singapore, 2017; pp. 109–155. [Google Scholar] [CrossRef]
  28. Talukder, B.; Das, B.K. A new invasive species of Kerria Targioni-Tozzetti (Hemiptera: Coccomorpha: Kerriidae) on rain tree, Albizia saman (Fabaceae) from India. Orient. Insects 2020, 54, 176–190. [Google Scholar] [CrossRef]
  29. Kaushik, S.; Sharma, K.K.; Ramani, R.; Lakhanpaul, S. Detection of Wolbachia Phage (WO) in Indian Lac Insect [Kerria lacca (Kerr.)] and Its Implications. Indian J. Microbiol. 2018, 59, 237–240. [Google Scholar] [CrossRef]
  30. Sharma, K.K.; Ramani, R. An update on synoptic catalogue of lac insects (Homoptera: Tachardiidae). J. Bombay Nat. Hist. Soc. 1999, 96, 438–443. [Google Scholar]
  31. Chen, X.M.; Chen, H.; Zhao, M.; Yang, Z.X.; Feng, Y. Insect industrialization and prospect in commerce: A case of China. Entomol. Res. 2022, 52, 178–194. [Google Scholar] [CrossRef]
  32. Yusuf, M.; Aijaz, M.; Keserwani, N.; Ansari, N.H.; Ahmad, S. Ethnomedicinal, Pharmacological and Commercial Perspectives of Laccifer lacca Body Exudate (LBE). Lett. Appl. NanoBioSci. 2022, 12, 1–10. [Google Scholar] [CrossRef]
  33. Barrie, W.E. Shellac Based Skin Care Lotion. U.S. Patent US20150258010A1, 17 September 2015. [Google Scholar]
  34. Schwarz, K.; Keppler, J.; Theismann, E.M.; Knipp, J.; Fangmann, D.; Laudes, M.; Schreiber, S.; Wätzig, G. Shellac Microcapsule Formulations and Compositions for Topical Intestinal Delivery of Vitamin b3. U.S. Patent US20210275513A1, 9 September 2021. [Google Scholar]
  35. Santos, S.A.; Barrie, W.E.; Murphy, K.M. Cannabis Delivery with Protective Glaze Coating. U.S. Patent US20200323791A1, 15 October 2020. [Google Scholar]
  36. Cho, I.G. Chocolate Confectionery Preparation Method and Chocolate Confectionery Prepared Thereby. U.S. Patent US10448655b2, 22 October 2019. [Google Scholar]
  37. Orlowski, M. Shellac and Paclitaxel Coated Catheter Balloons. U.S. Patent US10293085B2, 21 May 2019. [Google Scholar]
  38. Ghantous, A.; Most, C.; McVay, R. Metal Cans Coated with Shellac-Containing Coatings. U.S. Patent US10392515B2, 27 August 2019. [Google Scholar]
  39. Shyam, K.C.; Manish, K.B.; Tapan, K.R.; Sumitesh, D.; Ranjan, K.S.; Yashabanta, N.S.; Ashit, K.P.; Vikas, C.S. Process for Synthesizing Reduced Graphene Oxide on a Substrate from Seedlac. U.S. Patent US9617159B2, 11 April 2017. [Google Scholar]
  40. Seidl, W.; Wache, S.; Stohr, W.; Zürn, S.; Riedmiller, J.; Schwarz, V. Cementitious System Comprising Accelerator Particles Coated with Crosslinked Shellac. U.S. Patent US9428419B2, 30 August 2016. [Google Scholar]
  41. Signorino, C.A.; Smith, T.L.; Levine, S. Shellac Enteric Coatings. U.S. Patent 20110269850A1, 3 November 2011. [Google Scholar]
  42. Durig, T.; Zong, Y. Stable Shellac Enteric Coating Formulation for Nutraceutical and Pharmaceutical Dosage Forms. U.S. Patent US20110002986A1, 6 June 2011. [Google Scholar]
  43. Diaz, M. Sprayable Hand Cleaner Made of Edible Shellac and Micellular Surfactants. U.S. Patent US20100144574A1, 10 June 2010. [Google Scholar]
  44. Rohmer, M. The mevalonate-independent methylerythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis, including carotenoids. Pure Appl. Chem. 1999, 71, 2279–2284. [Google Scholar] [CrossRef]
  45. Bede, J.C.; Teal, P.E.A.; Goodman, W.G.; Tobe, S.S. Biosynthetic Pathway of Insect Juvenile Hormone III in Cell Suspension Cultures of the Sedge Cyperus iria. Plant Physiol. 2001, 127, 584–593. [Google Scholar] [CrossRef] [PubMed]
  46. Hsieh, M.H.; Goodman, H.M. The Arabidopsis IspH Homolog Is Involved in the Plastid Nonmevalonate Pathway of Isoprenoid Biosynthesis. Plant Physiol. 2005, 138, 641–653. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Kandasamy, T.; Ekbal, S.; Kumari, K. SSH reveals different functional categories of putative resin synthesizing genes in the industrial insect, Kerria lacca (Kerr). J. Asia-Pac. Entomol. 2021, 24, 1187–1193. [Google Scholar] [CrossRef]
  48. Shamim, G.; Pandey, D.M.; Ramani, R.; Sharma, K.K. Identification of genes related to resin biosynthesis in the Indian lac insect, Kerria lacca (Hemiptera: Tachardiidae). Int. J. Trop. Insect Sci. 2014, 34, 149–155. [Google Scholar] [CrossRef]
  49. Zhang, H.; Li, Z.X. In vitro and in vivo characterization of a novel insect decaprenyl diphosphate synthase: A two-major step catalytic mechanism is proposed. Biochem. Biophys. Res. Commun. 2013, 442, 105–111. [Google Scholar] [CrossRef]
  50. Koyama, T. Molecular analysis of prenyl chain elongating enzymes. Biosci. Biotechnol. Biochem. 1999, 63, 1671–1676. [Google Scholar] [CrossRef]
  51. Ogura, K. Enzymatic aspects of isoprenoid chain elongation. Chem. Rev. 1998, 98, 1263–1276. [Google Scholar] [CrossRef]
  52. Sun, Z.J.; Li, Z.X. The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid. Insect Mol. Biol. 2018, 27, 824–834. [Google Scholar] [CrossRef]
  53. Gu, L.H.; Han, J.M.; Wood, J.D.; Chang, C.Y.Y.; Sun, Y. Sun-induced Chl fluorescence and its importance for biophysical modeling of photosynthesis based on light reactions. New Phytol. 2019, 223, 1179–1191. [Google Scholar] [CrossRef] [Green Version]
  54. Sperling, P.; Ternes, P.; Zank, T.K.; Heinz, E. The evolution of desaturases. Prostaglandins Leukot. Essent. Fat. Acids 2003, 68, 73–95. [Google Scholar] [CrossRef] [PubMed]
  55. Dallerac, R.; Labeur, C.; Jallon, J.M.; Knipple, D.C.; Roelofs, W.L.; Wicker, T.C. A Δ9 desaturase gene with a different substrate specificity is responsible for the cuticular diene hydrocarbon polymorphism in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 2000, 97, 9449–9454. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  56. Horne, I.; Gibb, N.; Damcevski, K.; Glover, K.; Haritos, V.S. Two conserved Z9-octadecanoic acid desaturases in the red flour beetle, Tribolium castaneum. Gene 2010, 468, 41–47. [Google Scholar] [CrossRef] [PubMed]
  57. Riddervold, M.H.; Tittiger, C.; Blomquist, G.J.; Borgeson, C.E. Biochemical and molecular characterizaton of house cricket (Acheta domesticus, Orthoptera: Gryllidae) Δ9 desaturase. Insect Biochem. Mol. Biol. 2002, 32, 1731–1740. [Google Scholar] [CrossRef] [PubMed]
  58. Luo, J.; Li, Z.; Ma, C.; Zhang, Z.; Hull, J.J.; Lei, C.; Jin, S.; Chen, L. Knockdown of a metathoracic scent gland desaturase enhances the production of (E)-4-oxo-2-hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis. Insect Mol. Biol. 2017, 26, 642–653. [Google Scholar] [CrossRef] [PubMed]
  59. Zeng, J.M.; Ye, W.F.; Noman, A.; Machado, R.A.R.; Lou, Y.G. The desaturase gene family is crucially required for fatty acid metabolism and survival of the brown planthopper, Nilaparvata lugens. Int. J. Mol. Sci. 2019, 20, 1369. [Google Scholar] [CrossRef] [Green Version]
  60. Haritos, V.S.; Horne, I.; Damcevski, K.; Glover, K.; Gibb, N.; Okada, S.; Hamberg, M. The convergent evolution of defensive polyacetylenic fatty acid biosynthesis genes in soldier beetles. Nat. Commun. 2012, 3, 1150. [Google Scholar] [CrossRef] [Green Version]
  61. Kayukawa, T.; Chen, B.; Hoshizaki, S.; Ishikawa, Y. Upregulation of a desaturase is associated with the enhancement of cold hardiness in the onion maggot, Delia antiqua. Insect Biochem. Mol. Biol. 2007, 37, 1160–1167. [Google Scholar] [CrossRef]
  62. Wang, Y.; da Cruz, T.C.; Pulfemuller, A.; Grégoire, S.; Ferveur, J.F.; Moussian, B. Inhibition of fatty acid desaturases in Drosophila melanogaster larvae blocks feeding and developmental progression. Arch. Insect Biochem. Physiol. 2016, 92, 6–23. [Google Scholar] [CrossRef]
  63. Bousquet, F.; Nojima, T.; Houot, B.; Chauvel, I.; Chaudy, S.; Dupas, S.; Yamamoto, D.; Ferveur, J.F. Expression of a desaturase gene, desat1, in neural and nonneural tissues separately affects perception and emission of sex pheromones in Drosophila. Proc. Natl. Acad. Sci. USA 2012, 109, 249–254. [Google Scholar] [CrossRef] [Green Version]
  64. Köhler, K.; Brunner, E.; Guan, X.L.; Boucke, K.; Greber, U.F.; Mohanty, S.; Barth, J.M.; Wenk, M.R.; Hafen, E. A combined proteomic and genetic analysis identifies a role for the lipid desaturase Desat1 in starvation-induced autophagy in Drosophila. Autophagy 2009, 5, 980–990. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Lowe, R.; Shirley, N.; Bleackley, M.; Dolan, S.; Shafee, T. Transcriptomics technologies. PLoS Comput. Biol. 2017, 13, e1005457. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Dong, Z.C.; Chen, Y. Transcriptomics: Advances and approaches. Sci. China Life Sci. 2013, 56, 960–967. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Dubey, N.K.; Goel, R.; Ranjan, A.; Idris, A.; Singh, S.K.; Bag, S.K.; Chandrashekar, K.; Pandey, K.D.; Singh, P.K.; Sawant, S.V. Comparative transcriptome analysis of Gossypium hirsutum L. in response to sap sucking insects: Aphid and whitefly. BMC Genom. 2013, 14, 241. [Google Scholar] [CrossRef]
  68. Fan, X.B.; Pang, R.; Li, W.X.; Ojha, A.; Li, D.; Zhang, W.Q. An overview of embryogenesis: External morphology and transcriptome profiling in the Hemipteran Insect Nilaparvata lugens. Front. Physiol. 2020, 11, 106. [Google Scholar] [CrossRef] [Green Version]
  69. Hao, K.; Jarwar, A.R.; Ullah, H.; Tu, X.B.; Nong, X.Q.; Zhang, Z.H. Transcriptome sequencing reveals potential mechanisms of the maternal effect on egg diapause induction of Locusta migratoria. Int. J. Mol. Sci. 2019, 20, 1974. [Google Scholar] [CrossRef] [Green Version]
  70. Chen, Y.R.; Jiang, T.; Zhu, J.; Xie, Y.C.; Tan, Z.C.; Chen, Y.H.; Tang, S.M.; Hao, B.F.; Wang, S.P.; Huang, J.S. Transcriptome sequencing reveals potential mechanisms of diapause preparation in bivoltine silkworm Bombyx mori (Lepidoptera: Bombycidae). Comp. Biochem. Physiol.-D Genom. Proteom. 2017, 24, 68–78. [Google Scholar] [CrossRef]
  71. Chen, J.; Guo, Y.S.; Huang, S.M.; Zhan, H.R.; Zhang, M.F.; Wang, J.W.; Shu, Y.H. Integration of transcriptome and proteome reveals molecular mechanisms underlying stress responses of the cutworm, Spodoptera litura, exposed to different levels of lead (Pb). Chemosphere 2021, 283, 131205. [Google Scholar] [CrossRef]
  72. Liu, Y.C.; Su, H.; Li, R.Q.; Li, X.T.; Xu, Y.S.; Dai, X.P.; Zhou, Y.Y.; Wang, H.B. Comparative transcriptome analysis of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) reveals novel insights into heat stress tolerance in insects. BMC Genom. 2017, 18, 974. [Google Scholar] [CrossRef] [Green Version]
  73. Kandasamy, T.; Ekbal, S.; Kumari, K.; Lohot, V.D.; Mohanasundaram, A.; Sharma, K.K. Unraveling bacterial diversity of the Indian Lac Insect Kerria lacca (Kerr) using next generation sequencing. Int. J. Trop. Insect Sci. 2022, 42, 2365–2372. [Google Scholar] [CrossRef]
  74. Sharma, K.K.; Jaiswal, A.K. Microbial flora associated with lac insects. In Recent Advances in Lac Culture; Kumar, K.K., Ramani, R., Sharma, K.K., Eds.; Indian Lac Research Institute: Ranchi, India, 2011; pp. 22–33. [Google Scholar]
  75. Shamim, G.; Ranjan, S.K.; Kandasamy, T.; Sharma, K.K.; Ramani, R. Bacterial flora associated with Kerria lacca (Kerr). Indian J. Entomol. 2017, 79, 41–48. [Google Scholar] [CrossRef]
  76. Vashishtha, A.; Sharama, K.K.; Lakhanpaul, S. Co-existence, phylogeny and putative role of Wolbachia and yeast-like symbiont (YLS) in Kerria lacca (Kerr). Curr. Microbiol. 2011, 63, 206–212. [Google Scholar] [CrossRef] [PubMed]
  77. Kandasamy, T.; Sharma, K.K. Interaction of lac insects with microbes. In Commercial Entomology; Kumar, A., Kumar, N., Chand, H., Eds.; New India Publishing Agency: New Delhi, India, 2019; pp. 129–139. [Google Scholar]
  78. Chen, D.Q.; Montllor, C.B.; Purcell, A.H. Fitness effects of two facultative endosymbiotic bacteria on the pea aphid, Acyrthosiphon pisum, and the blue alfalfa aphid, A. kondoi. Entomol. Exp. Appl. 2000, 95, 315–323. [Google Scholar] [CrossRef]
  79. Fukatsu, T.; Nikoh, N.; Kawai, R.; Koga, R. The secondary endosymbiotic bacterium of the pea aphid Acyrthosiphon pisum (Insecta: Homoptera). Appl. Environ. Microbiol. 2000, 66, 2748–2758. [Google Scholar] [CrossRef]
  80. Dillon, R.J.; Dillon, V.M. The gut bacteria of insects: Nonpathogenic interactions. Annu. Rev. Entomol. 2004, 49, 71–92. [Google Scholar] [CrossRef]
  81. Wu, H.X.; Chen, X.M.; Chen, H.; Lu, Q.; Yang, Z.X.; Ren, W.B.; Liu, J.; Shao, S.X.; Wang, C.; King-Jones, K. Variation and diversification of the microbiome of Schlechtendalia chinensis on two alternate host plants. PLoS ONE 2018, 13, e0200049. [Google Scholar] [CrossRef] [Green Version]
  82. Ahmad, A.; Kaushik, S.; Ramamurthy, V.V.; Lakhanpaul, S.; Ramani, R.; Sharma, K.K.; Vidyarthi, A.S. Mouthparts and stylet penetration of the lac insect Kerria lacca (Kerr) (Hemiptera: Tachardiidae). Arthropod Struct. Dev. 2012, 41, 435–441. [Google Scholar] [CrossRef]
  83. Kerr, J. Natural history of the insect which produces the gum lacca. Philos. Trans. R. Soc. 1782, 71, 374–382. [Google Scholar]
  84. Sangha, K.S.; Shera, P.S.; Sharma, S.; Kaur, R.; Thakur, A.; Kaur, H. Studies on the population dynamics and host range of Lac insect in different agro-climatic zones of Punjab (India). J. Entomol. Zool. Stud. 2019, 7, 183–189. [Google Scholar]
  85. Roonwal, M.L.; Raizada, M.B.; Chatterji, R.N.; Singh, B. Descriptive Account of the Host-Plants of the Lac Insect, Laccifer lacca (Kerr) and the Allied Plants in the Indian Region—Part 2; Indian Lac Cess Committee: Ranchi, India, 1958; p. 101. [Google Scholar]
  86. Ben-Dov, Y. A Systematic Catalogue of Eight Scale Insect Families (Hemiptera: Coccoidea) of the World: Aclerdidae, Asterolecaniidae, Beesoniidae, Carayonemidae, Conchaspididae, Dactylopiidae, Kerriidae and Lecanodiaspididae; Elsevier: Amsterdam, The Netherlands, 2006; p. 368. [Google Scholar]
  87. Chen, H.; Chen, X.M.; Feng, Y.; Yang, H.; He, R.; Zhang, W.F.; Yang, Z.X. Molecular phylogeny and biogeography of lac insects (Hemiptera: Kerriidae) inferred from nuclear and mitochondrial gene sequences. Mol. Biol. Rep. 2013, 40, 5943–5952. [Google Scholar] [CrossRef]
  88. Bhatnagar, P.; Prajapati, S.; Lodhi, B.; Bhalawi, R.; Aarmo, B.S. Studies on lac host plant occurrence in different Agro-climatic zones of Madhya Pradesh. Int. J. Ecol. Environ. Sci. 2020, 2, 197–204. [Google Scholar]
  89. Vaibhav, D.L.; Jyotirmoy, G.; Thamilarasi, K.; Mohanasundaram, A.; Rajagopal, N.N.; Thakur, V.V.; Sharma, K.K. Exploring the utilization potential of Ficus species for cultivation of lac insects (Kerria species) in India. Agric. Food 2019, 1, 1–6. [Google Scholar]
  90. Ferdousee, N.; Julker, N.M.; Hoque, A.; Mohiuddin, M. Lac production and its economic return to rural economy in Rajshahi Division, Bangladesh. In Proceedings of the International Conference on Environmental Aspects of Bangladesh (ICEAB10), Kitakyushu, Japan, 4 September 2010; pp. 69–72. [Google Scholar]
  91. Chen, Y.Q.; Li, Q.; Chen, Y.L.; Wang, S.M.; Yang, Y.C. Lac-production, arthropod biodiversity and abundance, and pesticide use in Yunnan Province, China. Trop. Ecol. 2010, 51, 255–263. [Google Scholar]
  92. Singh, J.P.; Jaiswal, A.K.; Monobrullah, M.D. Green lacewing (Chrysoperla zastrowi arabica) incidence on Indian lac insect (Kerria lacca). Indian J. Agric. Sci. 2011, 81, 1087–1089. [Google Scholar]
  93. Singh, J.P.; Jaiswal, A.K.; Monobrullah, M.D. Safety evaluation of some newer pesticides against lac insect (Kerria lacca) for managing predators. Indian J. Agric. Sci. 2011, 81, 465–469. [Google Scholar]
  94. Sarvade, S.; Panse, R.K.; Rajak, S.K.; Upadhyay, V.B. Impact of biotic and abiotic factors on lac production and peoples’ livelihood improvement in India-An overview. J. Appl. Nat. Sci. 2018, 10, 894–904. [Google Scholar] [CrossRef]
  95. Jaiswal, A.K.; Bhattacharya, A.; Kumar, S.; Singh, J.P. Evaluation of Bacillus thuringiensis Berliner subsp. kurstaki for management of lepidopteran pests of lac insect. Entomon 2008, 33, 65–69. [Google Scholar]
  96. Sharma, K.K.; Kumari, K.; Lakhanpaul, S. Super parasitism in Indian lac insect, Kerria lacca (Kerr) and its implication on fecundity and resin producing efficiency of its two strains. Entomon 2007, 32, 33. [Google Scholar]
Insects 13 01117 g001 550
Figure 1. Bivoltine life cycle of female Kerria lacca: E represents eggs, L1 first-instar larvae, L2 second-instar larvae, A1 early-adult, A2 mid-adult, and A3 late-adult stage; the summer generation of lac insect shows different forms of lac, and the winter generation shows the attachments via their lac test to the host plant [18,19].
Figure 1. Bivoltine life cycle of female Kerria lacca: E represents eggs, L1 first-instar larvae, L2 second-instar larvae, A1 early-adult, A2 mid-adult, and A3 late-adult stage; the summer generation of lac insect shows different forms of lac, and the winter generation shows the attachments via their lac test to the host plant [18,19].
Insects 13 01117 g001
Insects 13 01117 g002 550
Figure 2. Energy pyramid from levels I to IV of lac insect; source: Bashir et al. and Mishra and Kumar [19,26,27].
Figure 2. Energy pyramid from levels I to IV of lac insect; source: Bashir et al. and Mishra and Kumar [19,26,27].
Insects 13 01117 g002
Insects 13 01117 g003 550
Figure 3. A diagram showing potential biosynthetic pathways for lac resin and lac dye production in lac insects.
Figure 3. A diagram showing potential biosynthetic pathways for lac resin and lac dye production in lac insects.
Insects 13 01117 g003
Insects 13 01117 g004 550
Figure 4. Applications of natural lac products: (A) uses in industries, and (B) patents on the use of shellac in the last few years.
Figure 4. Applications of natural lac products: (A) uses in industries, and (B) patents on the use of shellac in the last few years.
Insects 13 01117 g004
Insects 13 01117 g005 550
Figure 5. Unique and common genes involved in lac resin production and secretion: (A) Bashir et al. [19], (B) Kandasamy et al. [47], and (C) Wang et al. [4].
Figure 5. Unique and common genes involved in lac resin production and secretion: (A) Bashir et al. [19], (B) Kandasamy et al. [47], and (C) Wang et al. [4].
Insects 13 01117 g005
Insects 13 01117 g006 550
Figure 6. A sketch exploring the mechanism involved in lac secretion: (A) host plant, (B) stick lac, including lac and lac insect stages, (C) female adult stages represent active lac secretion stages, and larval and male stages represent non-active lac secretion stages, (D) investigation of mechanisms based on expression levels, and (E) different lac production pathways as described in the literature.
Figure 6. A sketch exploring the mechanism involved in lac secretion: (A) host plant, (B) stick lac, including lac and lac insect stages, (C) female adult stages represent active lac secretion stages, and larval and male stages represent non-active lac secretion stages, (D) investigation of mechanisms based on expression levels, and (E) different lac production pathways as described in the literature.
Insects 13 01117 g006
Insects 13 01117 g007 550
Figure 7. Host plants of lac insects: (A) Cajanus cajan and (B) Schleichera oleosa with lac encrustation (red area), Yuanjiang Experimental Station, Institute of Highland Forest Science, Chinese Academy of Forestry, Yunnan Province, China.
Figure 7. Host plants of lac insects: (A) Cajanus cajan and (B) Schleichera oleosa with lac encrustation (red area), Yuanjiang Experimental Station, Institute of Highland Forest Science, Chinese Academy of Forestry, Yunnan Province, China.
Insects 13 01117 g007
Table
Table 1. Main difference between adult female and male of lac insects.
Table 1. Main difference between adult female and male of lac insects.
CharacterFemaleMale
Metamorphosispaurometabolousholometabolous
Bodylarger and degeneratedsmaller and not degenerated as female
Head, thorax, and abdomenindistinctdistinct
Eyesabsentpresent
Antennaevestigialwell-develop
Legsabsentpresent
Wingsabsentpresent/absent
Mouthpartspresentabsent
Movementenclosed in lac secretion
and neotenic		not enclosed in lac secretion and move freely
Table
Table 2. Host plants of lac-producing species of the genus Kerria.
Table 2. Host plants of lac-producing species of the genus Kerria.
SpeciesHost plantReferences
K. chinensisCajanus indicus, Schleichera sp., and some other host plants[86]
K. laccaZiziphus mauritiana, Butea monosperma, Ficus religiosa, F. indica, and some other host plants[15,87]
K. nepalensisLitchi chinensis, Dalbergia cochinchinensis, Ficus sp.[26]
K. pusanaZiziphus jujube, Butea frondosa, B. monosperma, Schleichera oleosa, Samanea saman, Acacia montana[3,15,86,87]
K. ruralisMallotus philippinensis, Pueraria tonkinensis[3,86]
K. sindicaAcacia arabica, Albizia lebbeck, Ziziphus jujube, Ficus racemosa, F. glabella[86,87]
K. yunnanensisDalbergia obtusifolia, D. assamica, D. mimosoides, Schleichera oleosa, Ficus microcarpa[86,87]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Bashir, N.H.; Chen, H.; Munir, S.; Wang, W.; Chen, H.; Sima, Y.-K.; An, J. Unraveling the Role of Lac Insects in Providing Natural Industrial Products. Insects 2022, 13, 1117. https://doi.org/10.3390/insects13121117

AMA Style

Bashir NH, Chen H, Munir S, Wang W, Chen H, Sima Y-K, An J. Unraveling the Role of Lac Insects in Providing Natural Industrial Products. Insects. 2022; 13(12):1117. https://doi.org/10.3390/insects13121117

Chicago/Turabian Style

Bashir, Nawaz Haider, Huanhuan Chen, Shahzad Munir, Weiwei Wang, Hang Chen, Yong-Kang Sima, and Jiandong An. 2022. "Unraveling the Role of Lac Insects in Providing Natural Industrial Products" Insects 13, no. 12: 1117. https://doi.org/10.3390/insects13121117

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop