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Diagnostics, Volume 4, Issue 1 (March 2014) – 3 articles , Pages 1-26

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Article
Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging
by Renata Madru, Pontus Svenmarker, Christian Ingvar, Freddy Ståhlberg, Stefan-Andersson Engels, Linda Knutsson and Sven-Erik Strand
Diagnostics 2014, 4(1), 13-26; https://doi.org/10.3390/diagnostics4010013 - 10 Mar 2014
Cited by 3 | Viewed by 7333
Abstract
Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and [...] Read more.
Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and design patient-specific treatments. To fully exploit the possibilities of hybrid imaging a hybrid probe compatible with each imaging technology is required. Here, we present a hybrid nanoprobe for triple modality MR/SPECT/Fluorescence imaging. Our imaging agent is comprised of superparamagnetic iron oxide nanoparticles (SPIONs), labeled with 99mTc and an Alexa fluorophore (AF), together forming 99mTc-AF-SPIONs. The agent was stable in human serum, and, after subcutaneous injection in the hind paw of Wistar rats, showed to be highly specific by accumulating in the sentinel lymph node. All three modalities clearly visualized the imaging agent. Our results show that a single imaging agent can be used for hybrid imaging. The use of a single hybrid contrast agent permits simultaneous hybrid imaging and, more conventionally, allow for single modality imaging at different time points. For example, a hybrid contrast agent enables pre-operative planning, intra-operative guidance, and post-operative evaluation with the same contrast agent. Full article
(This article belongs to the Collection Hybrid Imaging in Medicine)
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Editorial
Acknowledgement to Reviewers of Diagnostics in 2013
by Diagnostics Editorial Office
Diagnostics 2014, 4(1), 12; https://doi.org/10.3390/diagnostics4010012 - 28 Feb 2014
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Abstract
The editors of Diagnostics would like to express their sincere gratitude to the following reviewers for assessing manuscripts in 2013. [...] Full article
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Article
An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method
by Frank Eertmans, Veerle Bogaert, Tanita Van Poecke and Barbara Puype
Diagnostics 2014, 4(1), 1-11; https://doi.org/10.3390/diagnostics4010001 - 9 Jan 2014
Cited by 6 | Viewed by 8918
Abstract
Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of [...] Read more.
Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen. Full article
(This article belongs to the Collection Feature Papers)
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