Diagnostics

25 pages, 6387 KiB

Open AccessArticle

Multi-Techniques for Analyzing X-ray Images for Early Detection and Differentiation of Pneumonia and Tuberculosis Based on Hybrid Features

by Ibrahim Abdulrab Ahmed, Ebrahim Mohammed Senan, Hamzeh Salameh Ahmad Shatnawi, Ziad Mohammad Alkhraisha and Mamoun Mohammad Ali Al-Azzam

Diagnostics 2023, 13(4), 814; https://doi.org/10.3390/diagnostics13040814 - 20 Feb 2023

Cited by 12 | Viewed by 2276

Abstract

An infectious disease called tuberculosis (TB) exhibits pneumonia-like symptoms and traits. One of the most important methods for identifying and diagnosing pneumonia and tuberculosis is X-ray imaging. However, early discrimination is difficult for radiologists and doctors because of the similarities between pneumonia and [...] Read more.

An infectious disease called tuberculosis (TB) exhibits pneumonia-like symptoms and traits. One of the most important methods for identifying and diagnosing pneumonia and tuberculosis is X-ray imaging. However, early discrimination is difficult for radiologists and doctors because of the similarities between pneumonia and tuberculosis. As a result, patients do not receive the proper care, which in turn does not prevent the disease from spreading. The goal of this study is to extract hybrid features using a variety of techniques in order to achieve promising results in differentiating between pneumonia and tuberculosis. In this study, several approaches for early identification and distinguishing tuberculosis from pneumonia were suggested. The first proposed system for differentiating between pneumonia and tuberculosis uses hybrid techniques, VGG16 + support vector machine (SVM) and ResNet18 + SVM. The second proposed system for distinguishing between pneumonia and tuberculosis uses an artificial neural network (ANN) based on integrating features of VGG16 and ResNet18, before and after reducing the high dimensions using the principal component analysis (PCA) method. The third proposed system for distinguishing between pneumonia and tuberculosis uses ANN based on integrating features of VGG16 and ResNet18 separately with handcrafted features extracted by local binary pattern (LBP), discrete wavelet transform (DWT) and gray level co-occurrence matrix (GLCM) algorithms. All the proposed systems have achieved superior results in the early differentiation between pneumonia and tuberculosis. An ANN based on the features of VGG16 with LBP, DWT and GLCM (LDG) reached an accuracy of 99.6%, sensitivity of 99.17%, specificity of 99.42%, precision of 99.63%, and an AUC of 99.58%. Full article

(This article belongs to the Special Issue Using Artificial Intelligence for the Early Detection of Pneumonia and Its Further Management)

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15 pages, 2637 KiB

Open AccessHypothesis

From Pathogens to Cancer: Are Cancer Cells Evolved Mitochondrial Super Cells?

by Mario G. Balzanelli, Pietro Distratis, Rita Lazzaro, Van Hung Pham, Raffaele Del Prete, Adriana Mosca, Francesco Inchingolo, Sergey K. Aityan, Luigi Santacroce, Kieu C. D. Nguyen and Ciro Gargiulo Isacco

Diagnostics 2023, 13(4), 813; https://doi.org/10.3390/diagnostics13040813 - 20 Feb 2023

Cited by 1 | Viewed by 1847

Abstract

Life is based on a highly specific combination of atoms, metabolism, and genetics which eventually reflects the chemistry of the Universe which is composed of hydrogen, oxygen, nitrogen, sulfur, phosphorus, and carbon. The interaction of atomic, metabolic, and genetic cycles results in the [...] Read more.

Life is based on a highly specific combination of atoms, metabolism, and genetics which eventually reflects the chemistry of the Universe which is composed of hydrogen, oxygen, nitrogen, sulfur, phosphorus, and carbon. The interaction of atomic, metabolic, and genetic cycles results in the organization and de-organization of chemical information of that which we consider as living entities, including cancer cells. In order to approach the problem of the origin of cancer it is therefore reasonable to start from the assumption that the sub-molecular level, the atomic structure, should be the considered starting point on which metabolism, genetics, and external insults eventually emanate. Second, it is crucial to characterize which of the entities and parts composing human cells may live a separate life; certainly, this theoretical standpoint would consider mitochondria, an organelle of “bacteria” origin embedded in conditions favorable for the onset of both. This organelle has not only been tolerated by immunity but has also been placed as a central regulator of cell defense. Virus, bacteria, and mitochondria are also similar in the light of genetic and metabolic elements; they share not only equivalent DNA and RNA features but also many basic biological activities. Thus, it is important to finalize that once the cellular integrity has been constantly broken down, the mitochondria like any other virus or bacteria return to their original autonomy to simply survive. The Warburg’s law that states the ability of cancers to ferment glucose in the presence of oxygen, indicates mitochondria respiration abnormalities may be the underlying cause of this transformation towards super cancer cells. Though genetic events play a key part in altering biochemical metabolism, inducing aerobic glycolysis, this is not enough to impair mitochondrial function since mitochondrial biogenesis and quality control are constantly upregulated in cancers. While some cancers have mutations in the nuclear-encoded mitochondrial tricarboxylic acid (TCA) cycle, enzymes that produce oncogenic metabolites, there is also a bio-physic pathway for pathogenic mitochondrial genome mutations. The atomic level of all biological activities can be considered the very beginning, marked by the electron abnormal behavior that consequently affects DNA of both cells and mitochondria. Whilst the cell’s nucleus DNA after a certain number of errors and defection tends to gradually switch off, the mitochondria DNA starts adopting several escape strategies, switching-on a few important genes that belong back at their original roots as independent beings. The ability to adopt this survival trick, by becoming completely immune to current life-threatening events, is probably the beginning of a differentiation process towards a “super-power cell”, the cancer cells that remind many pathogens, including virus, bacteria, and fungi. Thus, here, we present a hypothesis regarding those changes that first begin at the mitochondria atomic level to steadily involve molecular, tissue and organ levels in response to the virus or bacteria constant insults that drive a mitochondria itself to become an “immortal cancer cell”. Improved insights into this interplay between these pathogens and mitochondria progression may disclose newly epistemological paradigms as well as innovative procedures in targeting cancer cell progressive invasion. Full article

(This article belongs to the Section Pathology and Molecular Diagnostics)

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16 pages, 4925 KiB

Open AccessReview

Meta-Analysis of Cardiovascular Risk Factors in Offspring of Preeclampsia Pregnancies

by Weikai Wang, Ru Lin, Lan Yang, Yanxia Wang, Baohong Mao, Xiaoying Xu and Jing Yu

Diagnostics 2023, 13(4), 812; https://doi.org/10.3390/diagnostics13040812 - 20 Feb 2023

Cited by 2 | Viewed by 1345

Abstract

This study aimed to assess cardiovascular risk factors in the offspring of preeclampsia (PE) pregnancies. PubMed, Web of Science, Ovid, and other foreign language databases, as well as SinoMed, China National Knowledge Infrastructure, Wanfang, and China Science and Technology Journal Databases, were searched. [...] Read more.

This study aimed to assess cardiovascular risk factors in the offspring of preeclampsia (PE) pregnancies. PubMed, Web of Science, Ovid, and other foreign language databases, as well as SinoMed, China National Knowledge Infrastructure, Wanfang, and China Science and Technology Journal Databases, were searched. The case-control studies on cardiovascular risk factors in the offspring of PE pregnancies from 1 January 2010 to 31 December 2019 were collected. A random-effects model or a fixed-effects model was used, and RevMan 5.3 software was used for meta-analysis to determine the OR value and 95%CI of each cardiovascular risk factor. A total of 16 documents were included in this research, all of which were case-control studies, with a total of 4046 cases in the experimental group and 31,505 in the control group. The meta-analysis that was conducted demonstrated that SBP [MD = 1.51, 95%CI (1.15, 1.88)] and DBP [MD = 1.90, 95%CI (1.69, 2.10)] values in the PE pregnancy offspring group presented an elevation relative to the non-PE pregnancy offspring group. The total cholesterol value in the PE pregnancy offspring group presented an elevation relative to the non-PE pregnancy offspring group [MD = 0.11, 95%CI (0.08, 0.13)]. The low-density lipoprotein cholesterol value in the PE pregnancy offspring group was comparable to that in the non-PE pregnancy offspring group [MD = 0.01, 95%CI (−0.02, 0.05)]. The high-density lipoprotein cholesterol value in the PE pregnancy offspring group presented an elevation relative to the non-PE pregnancy offspring group [MD = 0.02, 95%CI (0.01, 0.03)]. The non-HDL cholesterol value in the PE pregnancy offspring group presented an elevation relative to the non-PE pregnancy offspring group [MD = 0.16, 95%CI (0.13, 0.19)]. The triglycerides [MD = −0.02, 95%CI (−0.03, −0.01)] and glucose [MD = −0.08, 95%CI (−0.09, −0.07)] values in the PE pregnancy offspring group presented a depletion relative to the non-PE pregnancy group. The insulin value in the PE pregnancy offspring group presented a depletion relative to the non-PE pregnancy offspring group [MD = −0.21, 95%CI (−0.32, −0.09)]. The BMI value in the PE pregnancy offspring group presented an elevation relative to the non-PE pregnancy offspring group [MD = 0.42, 95%CI (0.27, 0.57)]. In conclusion, dyslipidemia, elevated blood pressure, and increased BMI occur postpartum with PE, all of which are risk factors for cardiovascular diseases. Full article

(This article belongs to the Special Issue Implementing AI in Diagnosis of Cardiovascular Diseases)

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11 pages, 2915 KiB

Open AccessArticle

AI: Can It Make a Difference to the Predictive Value of Ultrasound Breast Biopsy?

by Jean L. Browne, Maria Ángela Pascual, Jorge Perez, Sulimar Salazar, Beatriz Valero, Ignacio Rodriguez, Darío Cassina, Juan Luis Alcázar, Stefano Guerriero and Betlem Graupera

Diagnostics 2023, 13(4), 811; https://doi.org/10.3390/diagnostics13040811 - 20 Feb 2023

Cited by 4 | Viewed by 1636

Abstract

(1) Background: This study aims to compare the ground truth (pathology results) against the BI-RADS classification of images acquired while performing breast ultrasound diagnostic examinations that led to a biopsy and against the result of processing the same images through the AI algorithm [...] Read more.

(1) Background: This study aims to compare the ground truth (pathology results) against the BI-RADS classification of images acquired while performing breast ultrasound diagnostic examinations that led to a biopsy and against the result of processing the same images through the AI algorithm KOIOS DS ^TM (KOIOS). (2) Methods: All results of biopsies performed with ultrasound guidance during 2019 were recovered from the pathology department. Readers selected the image which better represented the BI-RADS classification, confirmed correlation to the biopsied image, and submitted it to the KOIOS AI software. The results of the BI-RADS classification of the diagnostic study performed at our institution were set against the KOIOS classification and both were compared to the pathology reports. (3) Results: 403 cases were included in this study. Pathology rendered 197 malignant and 206 benign reports. Four biopsies on BI-RADS 0 and two images are included. Of fifty BI-RADS 3 cases biopsied, only seven rendered cancers. All but one had a positive or suspicious cytology; all were classified as suspicious by KOIOS. Using KOIOS, 17 B3 biopsies could have been avoided. Of 347 BI-RADS 4, 5, and 6 cases, 190 were malignant (54.7%). Because only KOIOS suspicious and probably malignant categories should be biopsied, 312 biopsies would have resulted in 187 malignant lesions (60%), but 10 cancers would have been missed. (4) Conclusions: KOIOS had a higher ratio of positive biopsies in this selected case study vis-à-vis the BI-RADS 4, 5 and 6 categories. A large number of biopsies in the BI-RADS 3 category could have been avoided. Full article

(This article belongs to the Section Medical Imaging and Theranostics)

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11 pages, 666 KiB

Open AccessArticle

Assessment of the Accuracy, Usability and Acceptability of a Rapid Test for the Simultaneous Diagnosis of Syphilis and HIV Infection in a Real-Life Scenario in the Amazon Region, Brazil

by Daniela Cristina Soares, Luciano Chaves Franco Filho, Herald Souza dos Reis, Yan Corrêa Rodrigues, Felipe Bonfim Freitas, Cintya de Oliveira Souza, Giseli Nogueira Damacena, Nazle Mendonça Collaço Véras, Pamela Cristina Gaspar, Adele Schwartz Benzaken, Joana da Felicidade Ribeiro Favacho, Olinda Macedo and Maria Luiza Bazzo

Diagnostics 2023, 13(4), 810; https://doi.org/10.3390/diagnostics13040810 - 20 Feb 2023

Cited by 2 | Viewed by 3323

Abstract

We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with [...] Read more.

We field-assessed the accuracy, acceptability, and feasibility of the SD BIOLINE HIV/Syphilis Duo rapid diagnostic test in three groups: pregnant women, female sex workers (FSW), and men who have sex with men (MSM). Venous blood samples collected in the field were compared with the respective gold standard methods: SD BIOLINE HIV/Syphilis Duo Treponemal Test versus FTA-abs (Wama brand) treponemal laboratory test for syphilis, and SD BIOLINE HIV/Syphilis Duo Test versus the fourth generation Genscreen Ultra HIV Ag-Ag (Bio-Rad brand) laboratory test for HIV. From a total of 529 participants, 397 (75.1%) were pregnant women, 76 (14.3%) FSW and 56 (10.6%) MSM. Sensitivity and specificity parameters of HIV were 100.0% (95% CI: 82.35–100.0%) and 100.0% (95% CI: 99.28–100.0%), respectively. Sensitivity and specificity parameters found for TP antibody detection were 95.00% (95% CI: 87.69–98.62%) and 100.0% (95% CI: 98.18–100.0%), respectively. The SD BIOLINE HIV/Syphilis Duo Test showed high acceptability among participants (85.87%) and health professionals (85.51%), as well as easy usability by professionals (91.06%). The usability of the SD BIOLINE HIV/Syphilis Duo Test kit would not be a barrier to accessing rapid testing, if the product were incorporated into the list of health service supplies. Full article

(This article belongs to the Section Point-of-Care Diagnostics and Devices)

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14 pages, 336 KiB

Open AccessReview

Advances in the Microbiological Diagnosis of Prosthetic Joint Infections

by Maria Eugenia Portillo and Ignacio Sancho

Diagnostics 2023, 13(4), 809; https://doi.org/10.3390/diagnostics13040809 - 20 Feb 2023

Cited by 7 | Viewed by 2235

Abstract

A significant number of prosthetic joint infections (PJI) are culture-negative and/or misinterpreted as aseptic failures in spite of the correct implementation of diagnostic culture techniques, such as tissue sample processing in a bead mill, prolonged incubation time, or sonication of removed implants. Misinterpretation [...] Read more.

A significant number of prosthetic joint infections (PJI) are culture-negative and/or misinterpreted as aseptic failures in spite of the correct implementation of diagnostic culture techniques, such as tissue sample processing in a bead mill, prolonged incubation time, or sonication of removed implants. Misinterpretation may lead to unnecessary surgery and needless antimicrobial treatment. The diagnostic value of non-culture techniques has been investigated in synovial fluid, periprosthetic tissues, and sonication fluid. Different feasible improvements, such as real-time technology, automated systems and commercial kits are now available to support microbiologists. In this review, we describe non-culture techniques based on nucleic acid amplification and sequencing methods. Polymerase chain reaction (PCR) is a frequently used technique in most microbiology laboratories which allows the detection of a nucleic acid fragment by sequence amplification. Different PCR types can be used to diagnose PJI, each one requiring the selection of appropriate primers. Henceforward, thanks to the reduced cost of sequencing and the availability of next-generation sequencing (NGS), it will be possible to identify the whole pathogen genome sequence and, additionally, to detect all the pathogen sequences present in the joint. Although these new techniques have proved helpful, strict conditions need to be observed in order to detect fastidious microorganisms and rule out contaminants. Specialized microbiologists should assist clinicians in interpreting the result of the analyses at interdisciplinary meetings. New technologies will gradually be made available to improve the etiologic diagnoses of PJI, which will remain an important cornerstone of treatment. Strong collaboration among all specialists involved is essential for the correct diagnosis of PJI. Full article

(This article belongs to the Special Issue Diagnosis and Treatment of Periprosthetic Joint Infections)

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Graphical abstract

20 pages, 1660 KiB

Open AccessReview

Airway Epithelium: A Neglected but Crucial Cell Type in Asthma Pathobiology

by Sabita Singh, Joytri Dutta, Archita Ray, Atmaja Karmakar and Ulaganathan Mabalirajan

Diagnostics 2023, 13(4), 808; https://doi.org/10.3390/diagnostics13040808 - 20 Feb 2023

Cited by 10 | Viewed by 1986

Abstract

The features of allergic asthma are believed to be mediated mostly through the Th2 immune response. In this Th2-dominant concept, the airway epithelium is presented as the helpless victim of Th2 cytokines. However, this Th2-dominant concept is inadequate to fill some of the [...] Read more.

The features of allergic asthma are believed to be mediated mostly through the Th2 immune response. In this Th2-dominant concept, the airway epithelium is presented as the helpless victim of Th2 cytokines. However, this Th2-dominant concept is inadequate to fill some of the vital knowledge gaps in asthma pathogenesis, like the poor correlation between airway inflammation and airway remodeling and severe asthma endotypes, including Th2-low asthma, therapy resistance, etc. Since the discovery of type 2 innate lymphoid cells in 2010, asthma researchers started believing in that the airway epithelium played a crucial role, as alarmins, which are the inducers of ILC2, are almost exclusively secreted by the airway epithelium. This underscores the eminence of airway epithelium in asthma pathogenesis. However, the airway epithelium has a bipartite functionality in sustaining healthy lung homeostasis and asthmatic lungs. On the one hand, the airway epithelium maintains lung homeostasis against environmental irritants/pollutants with the aid of its various armamentaria, including its chemosensory apparatus and detoxification system. Alternatively, it induces an ILC2-mediated type 2 immune response through alarmins to amplify the inflammatory response. However, the available evidence indicates that restoring epithelial health may attenuate asthmatic features. Thus, we conjecture that an epithelium-driven concept in asthma pathogenesis could fill most of the gaps in current asthma knowledge, and the incorporation of epithelial-protective agents to enhance the robustness of the epithelial barrier and the combative capacity of the airway epithelium against exogenous irritants/allergens may mitigate asthma incidence and severity, resulting in better asthma control. Full article

(This article belongs to the Special Issue State-of-the-Art Research on Asthma)

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18 pages, 4505 KiB

Open AccessSystematic Review

Diagnostic Performance of Two-Dimensional Ultrasound, Two-Dimensional Sonohysterography and Three-Dimensional Ultrasound in the Diagnosis of Septate Uterus—A Systematic Review and Meta-Analysis

by Juan Luis Alcázar, Isabel Carriles, María Belén Cajas, Susana Costa, Sofia Fabra, Maria Cabrero, Elena Castro, Aida Tomaizeh, María Victoria Laza, Alba Monroy, Irene Martinez, Maria Isabel Aguilar, Elena Hernani, Cristina Castellet, Agustin Oliva, María Ángela Pascual and Stefano Guerriero

Diagnostics 2023, 13(4), 807; https://doi.org/10.3390/diagnostics13040807 - 20 Feb 2023

Cited by 2 | Viewed by 1322

Abstract

Background: The septate uterus is the most common congenital uterine anomaly, and hysteroscopy is the gold standard for diagnosing it. The goal of this meta-analysis is to perform a pooled analysis of the diagnostic performance of two-dimensional transvaginal ultrasonography, two-dimensional transvaginal sonohysterography, three-dimensional [...] Read more.

Background: The septate uterus is the most common congenital uterine anomaly, and hysteroscopy is the gold standard for diagnosing it. The goal of this meta-analysis is to perform a pooled analysis of the diagnostic performance of two-dimensional transvaginal ultrasonography, two-dimensional transvaginal sonohysterography, three-dimensional transvaginal ultrasound, and three-dimensional transvaginal sonohysterography for the diagnosis of the septate uterus. Methods: Studies published between 1990 and 2022 were searched in PubMed, Scopus, and Web of Science. From 897 citations, we selected eighteen studies to include in this meta-analysis. Results: The mean prevalence of uterine septum in this meta-analysis was 27.8%. Pooled sensitivity and specificity were 83% and 99% for two-dimensional transvaginal ultrasonography (ten studies), 94% and 100% for two-dimensional transvaginal sonohysterography (eight studies), and 98% and 100% for three-dimensional transvaginal ultrasound (seven articles), respectively. The diagnostic accuracy of three-dimensional transvaginal sonohysterography was only described in two studies, and we did not calculate the pooled sensitivity and specificity for this method. Conclusion: Three-dimensional transvaginal ultrasound has the best performance capacity for the diagnosis of the septate uterus. Full article

(This article belongs to the Section Medical Imaging and Theranostics)

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14 pages, 1243 KiB

Open AccessReview

Diagnostic Performance Evaluation of Multiparametric Magnetic Resonance Imaging in the Detection of Prostate Cancer with Supervised Machine Learning Methods

by Hamide Nematollahi, Masoud Moslehi, Fahimeh Aminolroayaei, Maryam Maleki and Daryoush Shahbazi-Gahrouei

Diagnostics 2023, 13(4), 806; https://doi.org/10.3390/diagnostics13040806 - 20 Feb 2023

Cited by 6 | Viewed by 2028

Abstract

Prostate cancer is the second leading cause of cancer-related death in men. Its early and correct diagnosis is of particular importance to controlling and preventing the disease from spreading to other tissues. Artificial intelligence and machine learning have effectively detected and graded several [...] Read more.

Prostate cancer is the second leading cause of cancer-related death in men. Its early and correct diagnosis is of particular importance to controlling and preventing the disease from spreading to other tissues. Artificial intelligence and machine learning have effectively detected and graded several cancers, in particular prostate cancer. The purpose of this review is to show the diagnostic performance (accuracy and area under the curve) of supervised machine learning algorithms in detecting prostate cancer using multiparametric MRI. A comparison was made between the performances of different supervised machine-learning methods. This review study was performed on the recent literature sourced from scientific citation websites such as Google Scholar, PubMed, Scopus, and Web of Science up to the end of January 2023. The findings of this review reveal that supervised machine learning techniques have good performance with high accuracy and area under the curve for prostate cancer diagnosis and prediction using multiparametric MR imaging. Among supervised machine learning methods, deep learning, random forest, and logistic regression algorithms appear to have the best performance. Full article

(This article belongs to the Special Issue Artificial Intelligence in Clinical Medical Imaging Analysis)

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12 pages, 1810 KiB

Open AccessArticle

Role of Preoperative Ultrasound Shear-Wave Elastography and Radiofrequency-Based Arterial Wall Tracking in Assessing the Vulnerability of Carotid Plaques: Preliminary Results

by Daniela Mazzaccaro, Matteo Giannetta, Fabiana Fancoli, Giulia Matrone, Nicoletta Curcio, Michele Conti, Paolo Righini and Giovanni Nano

Diagnostics 2023, 13(4), 805; https://doi.org/10.3390/diagnostics13040805 - 20 Feb 2023

Viewed by 1398

Abstract

We aimed at evaluating the ability of point shear-wave elastography (pSWE) and of a radiofrequency (RF) echo-tracking-based method in preoperatively assessing the vulnerability of the carotid plaque in patients undergoing carotid endarterectomy (CEA) for significant asymptomatic stenosis. All patients who underwent CEA from [...] Read more.

We aimed at evaluating the ability of point shear-wave elastography (pSWE) and of a radiofrequency (RF) echo-tracking-based method in preoperatively assessing the vulnerability of the carotid plaque in patients undergoing carotid endarterectomy (CEA) for significant asymptomatic stenosis. All patients who underwent CEA from 03/2021 to 03/2022 performed a preoperative pSWE and an RF echo-based wall evaluation of arterial stiffness using an Esaote MyLab ultrasound system (EsaoteTM, Genova, Italy) with dedicated software. The data derived from these evaluations (Young’s modulus (YM), augmentation index (AIx), pulse-wave velocity (PWV)) were correlated with the outcome of the analysis of the plaque removed during the surgery. Data were analyzed on 63 patients (33 vulnerable and 30 stable plaques). In stable plaques, YM was significantly higher than in vulnerable plaques (49.6 + 8.1 kPa vs. 24.6 + 4.3 kPa, p = 0.009). AIx also tended to be slightly higher in stable plaques, even if it was not statistically significant (10.4 + 0.9% vs. 7.7 + 0.9%, p = 0.16). The PWV was similar (12.2 + 0.9 m/s for stable plaques vs. 10.6 + 0.5 m/s for vulnerable plaques, p = 0.16). For YM, values >34 kPa had a sensitivity of 50% and a specificity of 73.3% in predicting plaque nonvulnerability (area under the curve = 0.66). Preoperative measurement of YM by means of pSWE could be a noninvasive and easily applicable tool for assessing the preoperative risk of plaque vulnerability in asymptomatic patients who are candidates for CEA. Full article

(This article belongs to the Collection Vascular Diseases Diagnostics)

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13 pages, 1795 KiB

Open AccessArticle

Correlation of sLOX-1 Levels and MR Characteristics of Culprit Plaques in Intracranial Arteries with Stroke Recurrence

by Kaixuan Ren, Huayun Jiang, Tiantian Li, Chengqun Qian, Li Zhu and Tianle Wang

Diagnostics 2023, 13(4), 804; https://doi.org/10.3390/diagnostics13040804 - 20 Feb 2023

Cited by 2 | Viewed by 1346

Abstract

(1) Background: Symptomatic intracranial artery atherosclerosis (sICAS) is an important cause of acute ischaemic stroke (AIS) and is associated with a high risk of stroke recurrence. High-resolution magnetic resonance vessel wall imaging (HR-MR-VWI) is an effective method for evaluating atherosclerotic plaque characteristics. Soluble [...] Read more.

(1) Background: Symptomatic intracranial artery atherosclerosis (sICAS) is an important cause of acute ischaemic stroke (AIS) and is associated with a high risk of stroke recurrence. High-resolution magnetic resonance vessel wall imaging (HR-MR-VWI) is an effective method for evaluating atherosclerotic plaque characteristics. Soluble lectin-like oxidised low-density lipoprotein receptor-1 (sLOX-1) is closely associated with plaque formation and rupture. We aim to explore the correlation between sLOX-1 levels and culprit plaque characteristics, based on HR-MR-VWI, with stroke recurrence in patients with sICAS. (2) Methods: A total of 199 patients with sICAS underwent HR-MR-VWI between June 2020 and June 2021 in our hospital. The culprit vessel and plaque characteristics were assessed according to HR-MR-VWI, and sLOX-1 levels were measured by ELISA (enzyme linked immunosorbent assay). Outpatient follow-up was performed 3, 6, 9, and 12 months after discharge. (3) Results: sLOX-1 levels were significantly higher in the recurrence group than in the non-recurrence group (p < 0.001). The culprit plaque thickness, degree of stenosis and plaque burden were higher in the recurrence group than in the non-recurrence group (p = 0.003, p = 0.014 and p = 0.010, respectively). The incidence of hyperintensity on T1WI, positive remodelling and significant enhancement (p < 0.001, p = 0.003 and p = 0.027, respectively) was higher in the recurrence group than in the non-recurrence group. Kaplan–Meier curves showed that patients with sLOX-1 levels > 912.19 pg/mL and hyperintensity on T1WI in the culprit plaque had a higher risk of stroke recurrence (both p < 0.001). Multivariate Cox regression analysis showed that sLOX-1 > 912.19 pg/mL (HR = 2.583, 95%CI 1.142, 5.846, p = 0.023) and hyperintensity on T1WI in the culprit plaque (HR = 2.632, 95% CI 1.197, 5.790, p = 0.016) were independent risk factors for stroke recurrence. sLOX-1 levels were significantly associated with the culprit plaque thickness (r = 0.162, p = 0.022), degree of stenosis (r = 0.217, p = 0.002), plaque burden (r = 0.183, p = 0.010), hyperintensity on T1WI (F = 14.501, p < 0.001), positive remodelling (F = 9.602, p < 0.001), and significant enhancement (F = 7.684, p < 0.001) (4) Conclusions: sLOX-1 levels were associated with vulnerability of the culprit plaque and can be used as a supplement to HR-MR-VWI to predict stroke recurrence. Full article

(This article belongs to the Special Issue Advances in the Imaging of Stroke and Neurodegenerative Disorders)

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16 pages, 610 KiB

Open AccessReview

Diagnostic Methods for Evaluation of Gastric Motility—A Mini Review

by Yan Wang, Jiande D. Z. Chen and Borko Nojkov

Diagnostics 2023, 13(4), 803; https://doi.org/10.3390/diagnostics13040803 - 20 Feb 2023

Cited by 4 | Viewed by 3300

Abstract

Gastric motility abnormalities are common in patients with disorders of gut-brain interaction, such as functional dyspepsia and gastroparesis. Accurate assessment of the gastric motility in these common disorders can help understand the underlying pathophysiology and guide effective treatment. A variety of clinically applicable [...] Read more.

Gastric motility abnormalities are common in patients with disorders of gut-brain interaction, such as functional dyspepsia and gastroparesis. Accurate assessment of the gastric motility in these common disorders can help understand the underlying pathophysiology and guide effective treatment. A variety of clinically applicable diagnostic methods have been developed to objectively evaluate the presence of gastric dysmotility, including tests of gastric accommodation, antroduodenal motility, gastric emptying, and gastric myoelectrical activity. The aim of this mini review is to summarize the advances in clinically available diagnostic methods for evaluation of gastric motility and describe the advantages and disadvantages of each test. Full article

(This article belongs to the Special Issue Functional Gastrointestinal Disorders (FGIDs): Diagnosis and Pathophysiology)

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Graphical abstract

19 pages, 1398 KiB

Open AccessArticle

Diffuse Pulmonary Meningotheliomatosis: Clinic-Pathologic Entity or Indolent Metastasis from Meningioma (or Both)?

by Laura Melocchi, Giulio Rossi, Mirca Valli, Maria Cecilia Mengoli, Michele Mondoni, Luigi Lazzari-Agli, Giacomo Santandrea, Fabio Davoli, Chiara Baldovini, Alberto Cavazza and Thomas V. Colby

Diagnostics 2023, 13(4), 802; https://doi.org/10.3390/diagnostics13040802 - 20 Feb 2023

Cited by 3 | Viewed by 1801

Abstract

Pulmonary minute meningothelial-like nodules (MMNs) are common incidental findings in surgical specimens, consisting of tiny proliferation (usually no larger than 5–6 mm) of bland-looking meningothelial cells showing a perivenular and interstitial distribution, sharing morphologic, ultrastructural, and immunohistochemical profiles with meningiomas. The identification of [...] Read more.

Pulmonary minute meningothelial-like nodules (MMNs) are common incidental findings in surgical specimens, consisting of tiny proliferation (usually no larger than 5–6 mm) of bland-looking meningothelial cells showing a perivenular and interstitial distribution, sharing morphologic, ultrastructural, and immunohistochemical profiles with meningiomas. The identification of multiple bilateral MMNs leading to an interstitial lung disease characterized by diffuse and micronodular/miliariform patterns radiologically allows the diagnosis of diffuse pulmonary meningotheliomatosis (DPM). Nevertheless, the lung is the most common site of metastatic primary intracranial meningioma, and differential diagnosis with DPM may be impossible without clinic–radiologic integration. Herein, we report four cases (three females; mean age, 57.5 years) fitting the criteria of DPM, all incidentally discovered and histologically evidenced on transbronchial biopsy (2) and surgical resection (2). All cases showed immunohistochemical expression of epithelial membrane antigen (EMA), progesterone receptor, and CD56. Notably, three of these patients had a proven or radiologically suspected intracranial meningioma; in two cases, it was discovered before, and in one case, after the diagnosis of DPM. An extensive literature review (44 patients with DPM) revealed similar cases with imaging studies excluding intracranial meningioma in only 9% (4 of 44 cases studied). The diagnosis of DPM requires close correlation with the clinic–radiologic data since a subset of cases coexist with or follow a previously diagnosed intracranial meningioma and, thus, may represent incidental and indolent metastatic deposits of meningioma. Full article

(This article belongs to the Special Issue Advances in the Diagnosis and Management of Tumors/Cancers)

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19 pages, 3190 KiB

Open AccessArticle

Alzheimer Disease Classification through Transfer Learning Approach

by Noman Raza, Asma Naseer, Maria Tamoor and Kashif Zafar

Diagnostics 2023, 13(4), 801; https://doi.org/10.3390/diagnostics13040801 - 20 Feb 2023

Cited by 13 | Viewed by 3306

Abstract

Alzheimer’s disease (AD) is a slow neurological disorder that destroys the thought process, and consciousness, of a human. It directly affects the development of mental ability and neurocognitive functionality. The number of patients with Alzheimer’s disease is increasing day by day, especially in [...] Read more.

Alzheimer’s disease (AD) is a slow neurological disorder that destroys the thought process, and consciousness, of a human. It directly affects the development of mental ability and neurocognitive functionality. The number of patients with Alzheimer’s disease is increasing day by day, especially in old aged people, who are above 60 years of age, and, gradually, it becomes cause of their death. In this research, we discuss the segmentation and classification of the Magnetic resonance imaging (MRI) of Alzheimer’s disease, through the concept of transfer learning and customizing of the convolutional neural network (CNN) by specifically using images that are segmented by the Gray Matter (GM) of the brain. Instead of training and computing the proposed model accuracy from the start, we used a pre-trained deep learning model as our base model, and, after that, transfer learning was applied. The accuracy of the proposed model was tested over a different number of epochs, 10, 25, and 50. The overall accuracy of the proposed model was 97.84%. Full article

(This article belongs to the Special Issue AI/ML-Based Medical Image Processing and Analysis)

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20 pages, 4770 KiB

Open AccessArticle

A Deep Learning-Based Framework for Uncertainty Quantification in Medical Imaging Using the DropWeak Technique: An Empirical Study with Baresnet

by Mehmet Akif Cifci

Diagnostics 2023, 13(4), 800; https://doi.org/10.3390/diagnostics13040800 - 20 Feb 2023

Cited by 5 | Viewed by 2147

Abstract

Lung cancer is a leading cause of cancer-related deaths globally. Early detection is crucial for improving patient survival rates. Deep learning (DL) has shown promise in the medical field, but its accuracy must be evaluated, particularly in the context of lung cancer classification. [...] Read more.

Lung cancer is a leading cause of cancer-related deaths globally. Early detection is crucial for improving patient survival rates. Deep learning (DL) has shown promise in the medical field, but its accuracy must be evaluated, particularly in the context of lung cancer classification. In this study, we conducted uncertainty analysis on various frequently used DL architectures, including Baresnet, to assess the uncertainties in the classification results. This study focuses on the use of deep learning for the classification of lung cancer, which is a critical aspect of improving patient survival rates. The study evaluates the accuracy of various deep learning architectures, including Baresnet, and incorporates uncertainty quantification to assess the level of uncertainty in the classification results. The study presents a novel automatic tumor classification system for lung cancer based on CT images, which achieves a classification accuracy of 97.19% with an uncertainty quantification. The results demonstrate the potential of deep learning in lung cancer classification and highlight the importance of uncertainty quantification in improving the accuracy of classification results. This study’s novelty lies in the incorporation of uncertainty quantification in deep learning for lung cancer classification, which can lead to more reliable and accurate diagnoses in clinical settings. Full article

(This article belongs to the Section Machine Learning and Artificial Intelligence in Diagnostics)

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13 pages, 287 KiB

Open AccessArticle

White Matter Lesions Identified by Magnetic Resonance in Women with Migraine: A Volumetric Analysis and Clinical Correlations

by Natália de Oliveira Silva, Nicoly Machado Maciel, Júlio César Nather, Jr., Gabriela Ferreira Carvalho, Carina Ferreira Pinheiro, Marcelo Eduardo Bigal, Antônio Carlos dos Santos, Debora Bevilaqua-Grossi and Fabiola Dach

Diagnostics 2023, 13(4), 799; https://doi.org/10.3390/diagnostics13040799 - 20 Feb 2023

Viewed by 1222

Abstract

Background: Repeated migraine attacks and aura could independently cause structural changes in the central nervous system. Our research aims to study the correlation of migraine type, attack frequency, and other clinical variables with the presence, volume and localization of white matter lesions (WML), [...] Read more.

Background: Repeated migraine attacks and aura could independently cause structural changes in the central nervous system. Our research aims to study the correlation of migraine type, attack frequency, and other clinical variables with the presence, volume and localization of white matter lesions (WML), in a controlled study. Methods: Sixty volunteers from a tertiary headache center were selected and divided equally into four groups: episodic migraine without aura (MoA), episodic migraine with aura (MA), chronic migraine (CM) and controls (CG). Voxel-based morphometry techniques were used to analyze WML. Results: There were no differences in WML variables between groups. There was a positive correlation between age and the number and total volume of WMLs, which persisted in the comparison categorized by size and brain lobe. Disease duration was positively correlated with the number and total volume of WML, and when controlled by age, the correlation maintained significance only for the insular lobe. Aura frequency was associated with frontal and temporal lobe WMLs. There was no statistically significant correlation between WML and other clinical variables. Conclusion: Migraine overall is not a risk factor for WML. Aura frequency is, however, associated with temporal WML. Disease duration, in adjusted analyses that account for age, is associated with insular WML. Full article

(This article belongs to the Special Issue Advanced MRI in Clinical Diagnosis)

20 pages, 2955 KiB

Open AccessArticle

Innovation in Hyperinsulinemia Diagnostics with ANN-L(atin square) Models

by Nevena Rankovic, Dragica Rankovic and Igor Lukic

Diagnostics 2023, 13(4), 798; https://doi.org/10.3390/diagnostics13040798 - 20 Feb 2023

Cited by 1 | Viewed by 1212

Abstract

Hyperinsulinemia is a condition characterized by excessively high levels of insulin in the bloodstream. It can exist for many years without any symptomatology. The research presented in this paper was conducted from 2019 to 2022 in cooperation with a health center in Serbia [...] Read more.

Hyperinsulinemia is a condition characterized by excessively high levels of insulin in the bloodstream. It can exist for many years without any symptomatology. The research presented in this paper was conducted from 2019 to 2022 in cooperation with a health center in Serbia as a large cross-sectional observational study of adolescents of both genders using datasets collected from the field. Previously used analytical approaches of integrated and relevant clinical, hematological, biochemical, and other variables could not identify potential risk factors for developing hyperinsulinemia. This paper aims to present several different models using machine learning (ML) algorithms such as naive Bayes, decision tree, and random forest and compare them with a new methodology constructed based on artificial neural networks using Taguchi’s orthogonal vector plans (ANN-L), a special extraction of Latin squares. Furthermore, the experimental part of this study showed that ANN-L models achieved an accuracy of 99.5% with less than seven iterations performed. Furthermore, the study provides valuable insights into the share of each risk factor contributing to the occurrence of hyperinsulinemia in adolescents, which is crucial for more precise and straightforward medical diagnoses. Preventing the risk of hyperinsulinemia in this age group is crucial for the well-being of the adolescents and society as a whole. Full article

(This article belongs to the Section Machine Learning and Artificial Intelligence in Diagnostics)

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12 pages, 2793 KiB

Open AccessArticle

Retinal Vascular Tortuosity Index Change after Idiopathic Epiretinal Membrane Surgery: Does Internal Limiting Membrane Peeling Affect Retinal Vascular Tortuosity?

by Özge Yanık, Pınar Aydın Ellialtıoğlu, Sibel Demirel, Figen Batıoğlu and Emin Özmert

Diagnostics 2023, 13(4), 797; https://doi.org/10.3390/diagnostics13040797 - 20 Feb 2023

Cited by 1 | Viewed by 1240

Abstract

Background: Idiopathic epiretinal membrane (iERM) surgery is one of the most commonly performed vitreoretinal surgeries, and the issue of internal limiting membrane (ILM) peeling in ERM surgery is still controversial. The aims of this study are to evaluate the changes in retinal vascular [...] Read more.

Background: Idiopathic epiretinal membrane (iERM) surgery is one of the most commonly performed vitreoretinal surgeries, and the issue of internal limiting membrane (ILM) peeling in ERM surgery is still controversial. The aims of this study are to evaluate the changes in retinal vascular tortuosity index (RVTI) after pars plana vitrectomy for the iERM using optical coherence tomography angiography (OCTA) and to assess whether ILM peeling has an additional effect on RVTI reduction. Methods: This study included25 eyes of 25 iERM patients who underwent ERM surgery. The ERM was removed without ILM peeling in 10 eyes (40.0%), and the ILM was peeled in addition to the ERM in 15 eyes (60.0%). The existence of the ILM after ERM peeling was checked with second staining in all eyes. Best corrected visual acuity (BCVA) and 6 × 6 mm en-face OCTA images were recorded before surgery and at the first month postoperatively. A skeleton model of the retinal vascular structure was created following Otsu binarization of en-face OCTA images using ImageJ software (1.52U). RVTI was calculated as the ratio of each vessel length to its Euclidean distance on the skeleton model using the Analyze Skeleton plug-in. Results: The mean RVTI declined from 1.220 ± 0.017 to 1.201 ± 0.020 (p = 0.036) in eyes with ILM peeling and from 1.230 ± 0.038 to 1.195 ± 0.024 in eyes without ILM peeling (p = 0.037). There was no difference between the groups in terms of postoperative RVTI (p = 0.494). A statistically significant correlation was found between postoperative RVTI and postoperative BCVA (rho = 0.408, p = 0.043). Conclusions: The RVTI is an indirect indicator of the traction created by the iERM on retinal microvascular structures, and it was effectively reduced after iERM surgery. The postoperative RVTIs were similar in cases who underwent iERM surgery with or without ILM peeling. Therefore, ILM peeling may not have an additive effect on the loosening of microvascular traction and thus may be reserved for recurrent ERM surgeries. Full article

(This article belongs to the Special Issue Advances in Optical Coherence Tomography and Optical Coherence Tomography Angiography in Diseases of the Retina and Choroid)

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19 pages, 4910 KiB

Open AccessArticle

A Novel Proposal for Deep Learning-Based Diabetes Prediction: Converting Clinical Data to Image Data

by Muhammet Fatih Aslan and Kadir Sabanci

Diagnostics 2023, 13(4), 796; https://doi.org/10.3390/diagnostics13040796 - 20 Feb 2023

Cited by 9 | Viewed by 2838

Abstract

Diabetes, one of the most common diseases worldwide, has become an increasingly global threat to humans in recent years. However, early detection of diabetes greatly inhibits the progression of the disease. This study proposes a new method based on deep learning for the [...] Read more.

Diabetes, one of the most common diseases worldwide, has become an increasingly global threat to humans in recent years. However, early detection of diabetes greatly inhibits the progression of the disease. This study proposes a new method based on deep learning for the early detection of diabetes. Like many other medical data, the PIMA dataset used in the study contains only numerical values. In this sense, the application of popular convolutional neural network (CNN) models to such data are limited. This study converts numerical data into images based on the feature importance to use the robust representation of CNN models in early diabetes diagnosis. Three different classification strategies are then applied to the resulting diabetes image data. In the first, diabetes images are fed into the ResNet18 and ResNet50 CNN models. In the second, deep features of the ResNet models are fused and classified with support vector machines (SVM). In the last approach, the selected fusion features are classified by SVM. The results demonstrate the robustness of diabetes images in the early diagnosis of diabetes. Full article

(This article belongs to the Special Issue Artificial Intelligence Technology in Clinical Classification and Prediction)

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9 pages, 765 KiB

Open AccessArticle

Timing of Early Postoperative MRI following Primary Glioblastoma Surgery—A Retrospective Study of Contrast Enhancements in 311 Patients

by Alexander Malcolm Rykkje, Vibeke Andrée Larsen, Jane Skjøth-Rasmussen, Michael Bachmann Nielsen, Jonathan Frederik Carlsen and Adam Espe Hansen

Diagnostics 2023, 13(4), 795; https://doi.org/10.3390/diagnostics13040795 - 20 Feb 2023

Cited by 2 | Viewed by 1272

Abstract

An early postoperative MRI is recommended following Glioblastoma surgery. This retrospective, observational study aimed to investigate the timing of an early postoperative MRI among 311 patients. The patterns of the contrast enhancement (thin linear, thick linear, nodular, and diffuse) and time from surgery [...] Read more.

An early postoperative MRI is recommended following Glioblastoma surgery. This retrospective, observational study aimed to investigate the timing of an early postoperative MRI among 311 patients. The patterns of the contrast enhancement (thin linear, thick linear, nodular, and diffuse) and time from surgery to the early postoperative MRI were recorded. The primary endpoint was the frequencies of the different contrast enhancements within and beyond the 48-h from surgery. The time dependence of the resection status and the clinical parameters were analysed as well. The frequency of the thin linear contrast enhancements significantly increased from 99/183 (50.8%) within 48-h post-surgery to 56/81 (69.1%) beyond 48-h post-surgery. Similarly, MRI scans with no contrast enhancements significantly declined from 41/183 (22.4%) within 48-h post-surgery to 7/81 (8.6%) beyond 48-h post-surgery. No significant differences were found for the other types of contrast enhancements and the results were robust in relation to the choice of categorisation of the postoperative periods. Both the resection status and the clinical parameters were not statistically different in patients with an MRI performed before and after 48 h. The findings suggest that surgically induced contrast enhancements are less frequent when an early postoperative MRI is performed earlier than 48-h, supporting the recommendation of a 48-h window for an early postoperative MRI. Full article

(This article belongs to the Special Issue Advances in the Diagnostics and Therapies of Glioma)

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10 pages, 1298 KiB

Open AccessArticle

Deep Learning-Based Image Quality Improvement in Digital Positron Emission Tomography for Breast Cancer

by Mio Mori, Tomoyuki Fujioka, Mayumi Hara, Leona Katsuta, Yuka Yashima, Emi Yamaga, Ken Yamagiwa, Junichi Tsuchiya, Kumiko Hayashi, Yuichi Kumaki, Goshi Oda, Tsuyoshi Nakagawa, Iichiroh Onishi, Kazunori Kubota and Ukihide Tateishi

Diagnostics 2023, 13(4), 794; https://doi.org/10.3390/diagnostics13040794 - 20 Feb 2023

Viewed by 1267

Abstract

We investigated whether ¹⁸F-fluorodeoxyglucose positron emission tomography (PET)/computed tomography images restored via deep learning (DL) improved image quality and affected axillary lymph node (ALN) metastasis diagnosis in patients with breast cancer. Using a five-point scale, two readers compared the image quality of [...] Read more.

We investigated whether ¹⁸F-fluorodeoxyglucose positron emission tomography (PET)/computed tomography images restored via deep learning (DL) improved image quality and affected axillary lymph node (ALN) metastasis diagnosis in patients with breast cancer. Using a five-point scale, two readers compared the image quality of DL-PET and conventional PET (cPET) in 53 consecutive patients from September 2020 to October 2021. Visually analyzed ipsilateral ALNs were rated on a three-point scale. The standard uptake values SUV_max and SUV_peak were calculated for breast cancer regions of interest. For “depiction of primary lesion”, reader 2 scored DL-PET significantly higher than cPET. For “noise”, “clarity of mammary gland”, and “overall image quality”, both readers scored DL-PET significantly higher than cPET. The SUV_max and SUV_peak for primary lesions and normal breasts were significantly higher in DL-PET than in cPET (p < 0.001). Considering the ALN metastasis scores 1 and 2 as negative and 3 as positive, the McNemar test revealed no significant difference between cPET and DL-PET scores for either reader (p = 0.250, 0.625). DL-PET improved visual image quality for breast cancer compared with cPET. SUV_max and SUV_peak were significantly higher in DL-PET than in cPET. DL-PET and cPET exhibited comparable diagnostic abilities for ALN metastasis. Full article

(This article belongs to the Special Issue The Impact of PET/CT Imaging in Oncology)

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19 pages, 15748 KiB

Open AccessReview

Management of Non-Melanoma Skin Cancer: Radiologists Challenging and Risk Assessment

by Gaetano Maria Russo, Anna Russo, Fabrizio Urraro, Fabrizio Cioce, Luigi Gallo, Maria Paola Belfiore, Angelo Sangiovanni, Stefania Napolitano, Teresa Troiani, Pasquale Verolino, Antonello Sica, Gabriella Brancaccio, Giulia Briatico, Valerio Nardone and Alfonso Reginelli

Diagnostics 2023, 13(4), 793; https://doi.org/10.3390/diagnostics13040793 - 20 Feb 2023

Cited by 2 | Viewed by 1896

Abstract

Basal cell carcinoma, squamous cell carcinoma, and Merkel cell carcinoma are the three main types of nonmelanoma skin cancers and their rates of occurrence and mortality have been steadily rising over the past few decades. For radiologists, it is still difficult to treat [...] Read more.

Basal cell carcinoma, squamous cell carcinoma, and Merkel cell carcinoma are the three main types of nonmelanoma skin cancers and their rates of occurrence and mortality have been steadily rising over the past few decades. For radiologists, it is still difficult to treat patients with advanced nonmelanoma skin cancer. Nonmelanoma skin cancer patients would benefit greatly from an improved diagnostic imaging-based risk stratification and staging method that takes into account patient characteristics. The risk is especially elevated among those who previously received systemic treatment or phototherapy. Systemic treatments, including biologic therapies and methotrexate (MTX), are effective in managing immune-mediated diseases; however, they may increase susceptibility to NMSC due to immunosuppression or other factors. Risk stratification and staging tools are crucial in treatment planning and prognostic evaluation. PET/CT appears more sensitive and superior to CT and MRI for nodal and distant metastasis as well as in surveillance after surgery. The patient treatment response improved with advent and utilization of immunotherapy and different immune-specific criteria are established to standardized evaluation criteria of clinical trials but none of them have been utilized routinely with immunotherapy. The advent of immunotherapy has also arisen new critical issues for radiologists, such as atypical response pattern, pseudo-progression, as well as immune-related adverse events that require early identification to optimize and improve patient prognosis and management. It is important for radiologists to have knowledge of the radiologic features site of the tumor, clinical stage, histological subtype, and any high-risk features to assess immunotherapy treatment response and immune-related adverse events. Full article

(This article belongs to the Special Issue Image-Guided Cancer Diagnosis and Therapy)

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11 pages, 2387 KiB

Open AccessArticle

Risk of Secondary Cancer after Adjuvant Tamoxifen Treatment for Ductal Carcinoma In Situ: A Nationwide Cohort Study in South Korea

by Dooreh Kim, Jooyoung Oh, Jeong-Ho Seok, Hye Sun Lee, Soyoung Jeon and Chang Ik Yoon

Diagnostics 2023, 13(4), 792; https://doi.org/10.3390/diagnostics13040792 - 20 Feb 2023

Viewed by 1475

Abstract

Endocrine therapy is the mainstay treatment for hormone receptor-positive ductal carcinoma in situ. The aim of this study was to examine the long-term secondary malignancy risk of tamoxifen therapy. The data of patients diagnosed with breast cancer between January 2007 and December 2015 [...] Read more.

Endocrine therapy is the mainstay treatment for hormone receptor-positive ductal carcinoma in situ. The aim of this study was to examine the long-term secondary malignancy risk of tamoxifen therapy. The data of patients diagnosed with breast cancer between January 2007 and December 2015 were retrieved from the database of the Health Insurance Review and Assessment Service of South Korea. The International Classification of Diseases, 10th revision, was used to track all-site cancers. Age at the time of surgery, chronic disease status, and type of surgery were considered covariates in the propensity score matching analysis. The median follow-up duration was 89 months. Forty-one patients in the tamoxifen group and nine in the control group developed endometrial cancer. The Cox regression hazard ratio model showed that tamoxifen therapy was the only significant predictor of the development of endometrial cancer (hazard ratio, 2.791; 95% confidence interval, 1.355–5.747; p = 0.0054). No other type of cancer was associated with long-term tamoxifen use. In consonance with the established knowledge, the real-world data in this study demonstrated that tamoxifen therapy is related to an increased incidence of endometrial cancer. Full article

(This article belongs to the Section Pathology and Molecular Diagnostics)

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12 pages, 2363 KiB

Open AccessArticle

Ultrasound Control of Cervical Regeneration after Large Loop Excision of the Transformation Zone: Results of an Innovative Measurement Technique

by Vincenzo Pinto, Miriam Dellino, Carla Mariaflavia Santarsiero, Gennaro Cormio, Vera Loizzi, Valentina Griseta, Antonella Vimercati, Gerardo Cazzato, Eliano Cascardi and Ettore Cicinelli

Diagnostics 2023, 13(4), 791; https://doi.org/10.3390/diagnostics13040791 - 20 Feb 2023

Cited by 2 | Viewed by 1548

Abstract

The objective of this research is to evaluate cervical regeneration after large loop excision of the transformation zone (LLETZ) through the identification of a new sonographic reference point at the level of the uterine margins. In the period March 2021–January 2022, a total [...] Read more.

The objective of this research is to evaluate cervical regeneration after large loop excision of the transformation zone (LLETZ) through the identification of a new sonographic reference point at the level of the uterine margins. In the period March 2021–January 2022, a total of 42 patients affected by CIN 2–3 were treated with LLETZ at the University Hospital of Bari (Italy). Before performing LLETZ, cervical length and volume were measured with trans-vaginal 3D ultrasound. From the multiplanar images, the cervical volume was obtained using the Virtual Organ Computer-aided AnaLysis (VOCAL™) program with manual contour mode. The line that connects the points where the common trunk of the uterine arteries reaches the uterus splitting into the ascending major branch and the cervical branch was considered as the upper limit of the cervical canal. From the acquired 3D volume, the length and the volume of the cervix were measured between this line and the external uterine os. Immediately after LLETZ, the removed cone was measured using Vernier’s caliper, and before fixation in formalin, the volume of the excised tissue was evaluated by the fluid displacement technique based on the Archimedes principle. The proportion of excised cervical volume was 25.50 ± 17.43%. The volume and the height of the excised cone were 1.61 ± 0.82 mL and 9.65 ± 2.49 mm corresponding to 14.74 ± 11.91% and 36.26 ± 15.49% of baseline values, respectively. The volume and length of the residual cervix were also assessed using 3D ultrasound up to the sixth month after excision. At 6 weeks, about 50% of cases reported an unchanged or lower cervical volume compared to the baseline pre-LLETZ values. The average percentage of volume regeneration in examined patients was equal to 9.77 ± 55.33%. In the same period, the cervical length regeneration rate was 69.41 ± 14.8%. Three months after LLETZ, a volume regeneration rate of 41.36 ± 28.31% was found. For the length, an average regeneration rate of 82.48 ± 15.25% was calculated. Finally, at 6 months, the percentage of regeneration of the excised volume was 90.99 ± 34.91%. The regrowth percentage of the cervical length was 91.07 ± 8.03%. The cervix measurement technique that we have proposed has the advantage of identifying an unequivocal reference point in 3D cervical measurement. Ultrasound 3D evaluation could be useful in the clinical practice to evaluate the cervical tissue deficit and express the “potential of cervical regeneration” as well as provide the surgeon useful information about the cervical length. Full article

(This article belongs to the Special Issue Health Diagnosis with Smart Wearable Devices and AI Driven Medical Cloud)

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11 pages, 646 KiB

Open AccessArticle

Cardiometabolic Phenotyping in Heart Failure: Differences between Patients with Reduced vs. Preserved Ejection Fraction

by Alessio Balletti, Nicolò De Biase, Lavinia Del Punta, Francesco Filidei, Silvia Armenia, Filippo Masi, Valerio Di Fiore, Matteo Mazzola, Alessandra Bacca, Frank L. Dini, Stefano Taddei, Stefano Masi and Nicola Riccardo Pugliese

Diagnostics 2023, 13(4), 790; https://doi.org/10.3390/diagnostics13040790 - 20 Feb 2023

Viewed by 1388

Abstract

Aims. We explored multiple cardiometabolic patterns, including inflammatory and congestive pathways, in patients with heart failure (HF). Methods and Results. We enrolled 270 HF patients with reduced (<50%, HFrEF; n = 96) and preserved (≥50%, HFpEF; n = 174) ejection fraction. In HFpEF, [...] Read more.

Aims. We explored multiple cardiometabolic patterns, including inflammatory and congestive pathways, in patients with heart failure (HF). Methods and Results. We enrolled 270 HF patients with reduced (<50%, HFrEF; n = 96) and preserved (≥50%, HFpEF; n = 174) ejection fraction. In HFpEF, glycated hemoglobin (Hb1Ac) seemed to be relevant in its relationship with inflammation as Hb1Ac positively correlated with high-sensitivity C-reactive protein (hs-CRP; Spearman’s rank correlation coefficient ρ = 0.180, p < 0.05). In HFrEF, we found a correlation between Hb1Ac and norepinephrine (ρ = 0.207, p < 0.05). In HFpEF, we found a positive correlation between Hb1Ac and congestion expressed as pulmonary B lines (ρ = 0.187, p < 0.05); the inverse correlation, although not significant, was found in HFrEF between Hb1Ac and N-terminal pro-B-type natriuretic peptide (ρ = 0.079) and between Hb1Ac and B lines (ρ = −0.051). In HFrEF, we found a positive correlation between E/e’ ratio and Hb1Ac (ρ = 0.203, p < 0.05) and a negative correlation between tricuspid annular systolic excursion (TAPSE)/echocardiographically measured systolic pulmonary artery pressure (sPAP) (TAPSE/sPAP ratio) (ρ = −0.205, p < 0.05) and Hb1Ac. In HFpEF, we found a negative correlation between TAPSE/sPAP ratio and uric acid (ρ = −0.216, p < 0.05). Conclusion. In HF patients, HFpEF and HFrEF phenotypes are characterized by different cardiometabolic indices related to distinct inflammatory and congestive pathways. Patients with HFpEF showed an important relationship between inflammatory and cardiometabolic parameters. Conversely, in HFrEF, there is a significant relationship between congestion and inflammation, while cardiometabolism appears not to influence inflammation, instead affecting sympathetic hyperactivation. Full article

(This article belongs to the Special Issue Diagnosis and Treatment of Cardiac Diseases)

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11 pages, 1833 KiB

Open AccessArticle

The Impact of Novel Reconstruction Algorithms on Calcium Scoring: Results on a Dedicated Cardiac CT Scanner

by Milán Vecsey-Nagy, Zsófia Jokkel, Ádám Levente Jermendy, Martin Nagy, Melinda Boussoussou, Borbála Vattay, Márton Kolossváry, Csaba Csobay-Novák, Sigal Amin-Spector, Béla Merkely and Bálint Szilveszter

Diagnostics 2023, 13(4), 789; https://doi.org/10.3390/diagnostics13040789 - 20 Feb 2023

Cited by 1 | Viewed by 1780

Abstract

Contemporary reconstruction algorithms yield the potential of reducing radiation exposure by denoising coronary computed tomography angiography (CCTA) datasets. We aimed to assess the reliability of coronary artery calcium score (CACS) measurements with an advanced adaptive statistical iterative reconstruction (ASIR-CV) and model-based adaptive filter [...] Read more.

Contemporary reconstruction algorithms yield the potential of reducing radiation exposure by denoising coronary computed tomography angiography (CCTA) datasets. We aimed to assess the reliability of coronary artery calcium score (CACS) measurements with an advanced adaptive statistical iterative reconstruction (ASIR-CV) and model-based adaptive filter (MBAF2) designed for a dedicated cardiac CT scanner by comparing them to the gold-standard filtered back projection (FBP) calculations. We analyzed non-contrast coronary CT images of 404 consecutive patients undergoing clinically indicated CCTA. CACS and total calcium volume were quantified and compared on three reconstructions (FBP, ASIR-CV, and MBAF2+ASIR-CV). Patients were classified into risk categories based on CACS and the rate of reclassification was assessed. Patients were categorized into the following groups based on FBP reconstructions: 172 zero CACS, 38 minimal (1–10), 87 mild (11–100), 57 moderate (101–400), and 50 severe (400<). Overall, 19/404 (4.7%) patients were reclassified into a lower-risk group with MBAF2+ASIR-CV, while 8 additional patients (27/404, 6.7%) shifted downward when applying stand-alone ASIR-CV. The total calcium volume with FBP was 7.0 (0.0–133.25) mm³, 4.0 (0.0–103.5) mm³ using ASIR-CV, and 5.0 (0.0–118.5) mm³ with MBAF2+ASIR-CV (all comparisons p < 0.001). The concomitant use of ASIR-CV and MBAF2 may allow the reduction of noise levels while maintaining similar CACS values as FBP measurements. Full article

(This article belongs to the Special Issue Clinical Factors for Prognosis and Survival of Cardiovascular Diseases)

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24 pages, 2956 KiB

Open AccessReview

How to Identify Advanced Fibrosis in Adult Patients with Non-Alcoholic Fatty Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH) Using Ultrasound Elastography—A Review of the Literature and Proposed Multistep Approach

by Madalina-Gabriela Taru, Lidia Neamti, Vlad Taru, Lucia Maria Procopciuc, Bogdan Procopet and Monica Lupsor-Platon

Diagnostics 2023, 13(4), 788; https://doi.org/10.3390/diagnostics13040788 - 19 Feb 2023

Cited by 7 | Viewed by 3446

Abstract

Non-alcoholic fatty liver disease (NAFLD), and its progressive form, non-alcoholic steatohepatitis (NASH), represent, nowadays, real challenges for the healthcare system. Liver fibrosis is the most important prognostic factor for NAFLD, and advanced fibrosis is associated with higher liver-related mortality rates. Therefore, the key [...] Read more.

Non-alcoholic fatty liver disease (NAFLD), and its progressive form, non-alcoholic steatohepatitis (NASH), represent, nowadays, real challenges for the healthcare system. Liver fibrosis is the most important prognostic factor for NAFLD, and advanced fibrosis is associated with higher liver-related mortality rates. Therefore, the key issues in NAFLD are the differentiation of NASH from simple steatosis and identification of advanced hepatic fibrosis. We critically reviewed the ultrasound (US) elastography techniques for the quantitative characterization of fibrosis, steatosis, and inflammation in NAFLD and NASH, with a specific focus on how to differentiate advanced fibrosis in adult patients. Vibration-controlled transient elastography (VCTE) is still the most utilized and validated elastography method for liver fibrosis assessment. The recently developed point shear wave elastography (pSWE) and two-dimensional shear wave elastography (2D-SWE) techniques that use multiparametric approaches could bring essential improvements to diagnosis and risk stratification. Full article

(This article belongs to the Special Issue The State of the Art in Elastography for Gastroenterology and Hepatology)

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11 pages, 922 KiB

Open AccessArticle

Neoductgenesis in Ductal Carcinoma In Situ Coexists with Morphological Abnormalities Characteristic for More Aggressive Tumor Biology

by Agnieszka Łazarczyk, Joanna Streb, Przemysław Hałubiec, Anna Streb-Smoleń, Robert Jach, Diana Hodorowicz-Zaniewska, Elżbieta Łuczyńska and Joanna Szpor

Diagnostics 2023, 13(4), 787; https://doi.org/10.3390/diagnostics13040787 - 19 Feb 2023

Cited by 1 | Viewed by 1378

Abstract

Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is generally indolent, however, could advance to invasive carcinoma in more than one-third of cases if left untreated. Thus, there is continuous research to find DCIS characteristics that would enable [...] Read more.

Ductal carcinoma in situ (DCIS) is a non-invasive form of breast cancer that is generally indolent, however, could advance to invasive carcinoma in more than one-third of cases if left untreated. Thus, there is continuous research to find DCIS characteristics that would enable clinicians to decide if it could be left without intensive treatment. Neoductgenesis (i.e., formation of the new duct of improper morphology) is a promising, but still not sufficiently evaluated indicator of future tumor invasiveness. We gathered data from 96 cases of DCIS (histopathological, clinical, and radiological) to assess the relationship between the neoductgenesis and well-established features of high-risk tumor behavior. Furthermore, our intention was to determine which degree of neoductgenesis should be considered clinically significant. Our major finding was that neoductgenesis is strictly related to other characteristics that indicate the invasive potential of the tumor and, to achieve more accurate prediction, neoductgenesis should be accordingly recognized to less strict criteria. Therefore, we conclude that neoductgenesis is another important revelator of tumor malignancy and that it requires further investigation during prospective controlled trials. Full article

(This article belongs to the Special Issue Diagnosis and Management of Gynecological Cancers: Volume 2)

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13 pages, 293 KiB

Open AccessArticle

Pressure Pain Thresholds and Central Sensitization in Relation to Psychosocial Predictors of Chronicity in Low Back Pain

by Anke Steinmetz, Franziska Hacke and Karl-Stefan Delank

Diagnostics 2023, 13(4), 786; https://doi.org/10.3390/diagnostics13040786 - 19 Feb 2023

Cited by 4 | Viewed by 2168

Abstract

(1) Background: Peripheral, as well as central, sensitization have been described in chronic low back pain (cLBP). The purpose of this study is to investigate the influence of psychosocial factors on the development of central sensitization. (2) Methods: This prospective study investigated local [...] Read more.

(1) Background: Peripheral, as well as central, sensitization have been described in chronic low back pain (cLBP). The purpose of this study is to investigate the influence of psychosocial factors on the development of central sensitization. (2) Methods: This prospective study investigated local and peripheral pressure pain thresholds and their dependence on psychosocial risk factors in patients with cLBP receiving inpatient multimodal pain therapy. Psychosocial factors were assessed using the Örebro Musculoskeletal Pain Screening Questionnaire (ÖMPSQ). (3) Results: A total of 90 patients were included in the study, 61 (75.4% women, 24.6% men) of whom had significant psychosocial risk factors. The control group consisted of 29 patients (62.1% women, 37.9% men). At baseline, patients with psychosocial risk factors showed significantly lower local and peripheral pressure pain thresholds, suggesting central sensitization, compared to the control group. Sleep quality, measured by the Pittsburgh Sleep Quality Index (PSQI), was also correlated with altered PPTs. After multimodal therapy, all participants reported increased local pain thresholds compared to at admission, independent of psychosocial chronification factors. (4) Conclusions: Psychosocial chronicity factors measured using the ÖMPSQ have a significant influence on pain sensitization in cLBP. A 14-day multimodal pain therapy increased local, but not peripheral, pressure pain thresholds. Full article

(This article belongs to the Special Issue Clinical Diagnosis and Treatment of Chronic Pain)

19 pages, 2407 KiB

Open AccessReview

The Role of Heart Rate Variability (HRV) in Different Hypertensive Syndromes

by Louise Buonalumi Tacito Yugar, Juan Carlos Yugar-Toledo, Nelson Dinamarco, Luis Gustavo Sedenho-Prado, Beatriz Vaz Domingues Moreno, Tatiane de Azevedo Rubio, Andre Fattori, Bruno Rodrigues, Jose Fernando Vilela-Martin and Heitor Moreno

Diagnostics 2023, 13(4), 785; https://doi.org/10.3390/diagnostics13040785 - 19 Feb 2023

Cited by 10 | Viewed by 5205

Abstract

Cardiac innervation by the parasympathetic nervous system (PNS) and the sympathetic nervous system (SNS) modulates the heart rate (HR) (chronotropic activity) and the contraction of the cardiac muscle (inotropic activity). The peripheral vasculature is controlled only by the SNS, which is responsible for [...] Read more.

Cardiac innervation by the parasympathetic nervous system (PNS) and the sympathetic nervous system (SNS) modulates the heart rate (HR) (chronotropic activity) and the contraction of the cardiac muscle (inotropic activity). The peripheral vasculature is controlled only by the SNS, which is responsible for peripheral vascular resistance. This also mediates the baroreceptor reflex (BR), which in turn mediates blood pressure (BP). Hypertension (HTN) and the autonomic nervous system (ANS) are closely related, such that derangements can lead to vasomotor impairments and several comorbidities, including obesity, hypertension, resistant hypertension, and chronic kidney disease. Autonomic dysfunction is also associated with functional and structural changes in target organs (heart, brain, kidneys, and blood vessels), increasing cardiovascular risk. Heart rate variability (HRV) is a method of assessing cardiac autonomic modulation. This tool has been used for clinical evaluation and to address the effect of therapeutic interventions. The present review aims (a) to approach the heart rate (HR) as a CV risk factor in hypertensive patients; (b) to analyze the heart rate variability (HRV) as a “tool” to estimate the individual risk stratum for Pre-HTN (P-HTN), Controlled-HTN (C-HTN), Resistant and Refractory HTN (R-HTN and Rf-HTN, respectively), and hypertensive patients with chronic renal disease (HTN+CKD). Full article

(This article belongs to the Special Issue New Progress in Diagnostics of Clinical Hypertension)

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Diagnostics, Volume 13, Issue 4 (February-2 2023) – 237 articles

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