Platycodon grandiflorus Polysaccharides Alleviate Cr(VI)-Induced Apoptosis in DF-1 Cells via ROS-Drp1 Signal Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Cell Culture and Treatment
2.3. Western Blot Analysis
2.4. Measurement of ROS
2.5. Measurement of MMP
2.6. Annexin V-FITC/PI Staining for Apoptosis Detection
2.7. Statistical Analysis
3. Results
3.1. PGPSt Alleviated Cr(VI)-Induced Mitochondrial Damage
3.2. Inhibition of Cr(VI) Induction of Mitochondria-Dependent Apoptosis by PGPSt
3.3. Effect of NAC on Mitochondrial Damage and Cellular Apoptosis in Cr(VI)-Exposed DF-1 Cells
3.4. Effect of Midivi-1 on Mitochondrial Damage and Cellular Apoptosis in Cr(VI)-Exposed DF-1 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Conflicts of Interest
References
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Zhang, Z.; Zheng, P.; Qi, C.; Cui, Y.; Qi, Y.; Xue, K.; Yan, G.; Liu, J. Platycodon grandiflorus Polysaccharides Alleviate Cr(VI)-Induced Apoptosis in DF-1 Cells via ROS-Drp1 Signal Pathway. Life 2022, 12, 2144. https://doi.org/10.3390/life12122144
Zhang Z, Zheng P, Qi C, Cui Y, Qi Y, Xue K, Yan G, Liu J. Platycodon grandiflorus Polysaccharides Alleviate Cr(VI)-Induced Apoptosis in DF-1 Cells via ROS-Drp1 Signal Pathway. Life. 2022; 12(12):2144. https://doi.org/10.3390/life12122144
Chicago/Turabian StyleZhang, Zhuanglong, Pimiao Zheng, Changxi Qi, Yuehui Cui, Yijian Qi, Kun Xue, Guangwei Yan, and Jianzhu Liu. 2022. "Platycodon grandiflorus Polysaccharides Alleviate Cr(VI)-Induced Apoptosis in DF-1 Cells via ROS-Drp1 Signal Pathway" Life 12, no. 12: 2144. https://doi.org/10.3390/life12122144