Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Standard Semen Analysis
2.2. Preparation of Sperm Subpopulations
2.3. Preparation of Media
2.3.1. HD-C Medium
2.3.2. Progesterone
2.3.3. Myo-Inositol
2.3.4. Dopamine
2.3.5. Prolactin
2.4. Viscosity
2.5. Sperm Morphology
2.6. Sperm Vitality
2.7. Sperm Motility, Concentration and Mucous Penetration
2.8. Hyperactivation
2.9. Reactive Oxygen Species
2.10. Mitochondrial Membrane Potential (ΔΨm)
2.11. Acrosome Reaction
2.12. Statistical Analysis
3. Results
3.1. Bubble Diagrams and Quantitative Results
3.2. Standard Semen Analysis
3.3. Vitality
3.4. Motility and Kinematic Parameters
3.5. Hyperactivation
3.6. Reactive Oxygen Species
3.7. Mitochondrial Membrane Potential
3.8. Acrosome Reaction
4. Discussion
4.1. Progesterone
4.2. Myo-Inositol
4.3. HD-C Medium
4.4. Dopamine
4.5. Prolactin
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
- Practice Committee of the American Society for Reproductive Medicine. Diagnostic evaluation of the infertile male: A committee opinion. Fertil. Steril. 2015, 103, e18–e25. [Google Scholar] [CrossRef]
- Hasdemir, P.S.; Goker, A. Alternative Approaches in the Management of Male Factor Infertility: A Contemporary Review. J. Obstet. Gynaecol. 2020, 4, 1–9. [Google Scholar]
- Miner, S.A.; Robins, S.; Zhu, Y.J.; Keeren, K.; Gu, V.; Read, S.C.; Zelkowitz, P. Evidence for the use of complementary and alternative medicines during fertility treatment: A scoping review. BMC Complement. Altern. Med. 2018, 18, 158. [Google Scholar] [CrossRef] [Green Version]
- European IVF-monitoring Consortium (EIM); European Society of Human Reproduction and Embryology (ESHRE); Calhaz-Jorge, C.; De Geyter, C.; Kupka, M.S.; de Mouzon, J.; Erb, K.; Mocanu, E.; Motrenko, T.; Scaravelli, G.; et al. Assisted reproductive technology in Europe, 2013: Results generated from European registers by ESHRE. Hum. Reprod. 2017, 32, 1957–1973. [Google Scholar] [CrossRef] [Green Version]
- Keyser, S.; van der Horst, G.; Maree, L. New approaches to define the functional competency of human sperm subpopulations and its relationship to semen quality. Int. J. Fertil. Steril. 2021. under review. [Google Scholar]
- Pujianto, D.A.; Curry, B.J.; Aitken, R.J. Prolactin Exerts a Prosurvival Effect on Human Spermatozoa via Mechanisms that Involve the Stimulation of Akt Phosphorylation and Suppression of Caspase Activation and Capacitation. Endocrinology 2009, 151, 1269–1279. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Condorelli, R.A.; La Vignera, S.; Mongioì, L.M.; Vitale, S.G.; Laganà, A.S.; Cimino, L.; E Calogero, A. Myo-inositol as a male fertility molecule: Speed them up! Eur. Rev. Med. Pharmacol. Sci. 2017, 21, 30–35. [Google Scholar]
- González, B.; Gancedo, S.N.; Garazatua, S.A.J.; Roldán, E.; Vitullo, A.D.; González, C.R. Dopamine Receptor D1 Contributes to Cocaine Epigenetic Reprogramming of Histone Modifications in Male Germ Cells. Front. Cell Dev. Biol. 2020, 8, 216. [Google Scholar] [CrossRef] [Green Version]
- Zhang, D.; Yuan, X.; Zhen, J.; Sun, Z.; Deng, C.; Yu, Q. Mildly Higher Serum Prolactin Levels Are Directly Proportional to Cumulative Pregnancy Outcomes in in-vitro Fertilization/Intracytoplasmic Sperm Injection Cycles. Front. Endocrinol. 2020, 11, 584. [Google Scholar] [CrossRef] [PubMed]
- Khatun, A.; Rahman, S.; Pang, M.-G. Clinical assessment of the male fertility. Obstet. Gynecol. Sci. 2018, 61, 179–191. [Google Scholar] [CrossRef]
- Correia, J.N. Novel Modulators of Human Sperm Motility and Migration. Doctoral Dissertation, University of Birmingham, Birgmingham, UK, 2012. [Google Scholar]
- Karaer, A.; Tuncay, G.; Mumcu, A.; Dogan, B. Metabolomics analysis of follicular fluid in women with ovarian endometriosis undergoing in vitro fertilization. Syst. Biol. Reprod. Med. 2018, 65, 39–47. [Google Scholar] [CrossRef]
- Ramírez, A.; Castro, M.A.; Angulo, C.; Ramió-Lluch, L.; Rivera, M.M.; Torres-Gutierrez, M.A.; Rigau, T.; Gil, J.E.R.; Concha, I.I. The Presence and Function of Dopamine Type 2 Receptors in Boar Sperm: A Possible Role for Dopamine in Viability, Capacitation, and Modulation of Sperm Motility1. Biol. Reprod. 2009, 80, 753–761. [Google Scholar] [CrossRef] [PubMed]
- Ren, D.; Xia, J. Calcium Signaling Through CatSper Channels in Mammalian Fertilization. Physiology 2010, 25, 165–175. [Google Scholar] [CrossRef] [Green Version]
- Scarselli, F.; Lobascio, A.M.; Terribile, M.; Casciani, V.; Greco, P.; Franco, G.; Minasi, M.G.; Greco, E. Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele. Arch. Ital. Urol. Androl. 2016, 88, 279–283. [Google Scholar] [CrossRef]
- Ramírez-Reveco, A.; Villarroel-Espíndola, F.; Gil, J.E.R.; Concha, I.I. Neuronal signaling repertoire in the mammalian sperm functionality. Biol. Reprod. 2017, 96, 505–524. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jung, C.; Fernández-Dueñas, V.; Plata, C.; Garcia-Elias, A.; Ciruela, F.; Fernández-Fernández, J.M.; Valverde, M.A. Functional coupling of GABA A/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct. Sci. Signal. 2018, 11, eaam6558. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Molina, L.C.P.; Luque, G.; Balestrini, P.A.; Marín-Briggiler, C.I.; Romarowski, A.; Buffone, M.G. Molecular Basis of Human Sperm Capacitation. Front. Cell Dev. Biol. 2018, 6, 72. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Diao, R.; Gan, H.; Tian, F.; Cai, X.; Zhen, W.; Song, X.; Duan, Y. In vitro antioxidation effect of Quercetin on sperm function from the infertile patients with leukocytospermia. Am. J. Reprod. Immunol. 2019, 82, e13155. [Google Scholar] [CrossRef]
- Rodríguez-Gil, J.E. Photostimulation and thermotaxis of sperm: Overview and practical implications in porcine reproduction. Theriogenology 2019, 137, 8–14. [Google Scholar] [CrossRef]
- Machado, S.A.; Sharif, M.; Wang, H.; Bovin, N.; Miller, D.J. Release of porcine sperm from oviduct cells is stimulated by pro-gesterone and requires CatSper. Sci. Rep. 2019, 9, 19546. [Google Scholar] [CrossRef] [Green Version]
- Tamburrino, L.; Marchiani, S.; Muratori, M.; Luconi, M.; Baldi, E. Progesterone, spermatozoa and reproduction: An updated review. Mol. Cell. Endocrinol. 2020, 516, 110952. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Kang, H.; Peng, L.; Song, D.; Jiang, X.; Li, Y.; Chen, H.; Zeng, X. Pentachlorophenol inhibits CatSper function to compromise progesterone’s action on human sperm. Chemosphere 2020, 259, 127493. [Google Scholar] [CrossRef]
- Smith, J.F.; Syritsyna, O.; Fellous, M.; Serres, C.; Mannowetz, N.; Kirichok, Y.; Lishko, P.V. Disruption of the principal, progesterone-activated sperm Ca2+ channel in a CatSper2-deficient infertile patient. Proc. Natl. Acad. Sci. USA 2013, 110, 6823–6828. [Google Scholar] [CrossRef] [Green Version]
- Vazquez-Levin, M.H.; Verón, G.L. Myo-inositol in health and disease: Its impact on semen parameters and male fertility. Andrology 2019, 8, 277–298. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Travis, A.J.; Kopf, G.S. The role of cholesterol efflux in regulating the fertilization potential of mammalian spermatozoa. J. Clin. Investig. 2002, 110, 731–736. [Google Scholar] [CrossRef] [PubMed]
- Santoro, M.; Aquila, S.; Russo, G. Sperm performance in oligoasthenoteratozoospermic patients is induced by a nutraceuticals mix, containing mainly myo-inositol. Syst. Biol. Reprod. Med. 2020, 67, 50–63. [Google Scholar] [CrossRef] [PubMed]
- Saller, S.; Kunz, L.; Berg, D.; Berg, U.; Lara, H.; Urra, J.; Hecht, S.; Pavlik, R.; Thaler, C.; Mayerhofer, A. Dopamine in human follicular fluid is associated with cellular uptake and metabolism-dependent generation of reactive oxygen species in granulosa cells: Implications for physiology and pathology. Hum. Reprod. 2013, 29, 555–567. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Urra, J.A.; Villaroel-Espíndola, F.; Covarrubias, A.A.; Rodríguez-Gil, J.E.; Ramirez-Reveco, A.; Concha, I.I. Presence and Function of Dopamine Transporter (DAT) in Stallion Sperm: Dopamine Modulates Sperm Motility and Acrosomal Integrity. PLoS ONE 2014, 9, e112834. [Google Scholar] [CrossRef] [Green Version]
- Raut, S.; Deshpande, S.; Balasinor, N.H. Unveiling the Role of Prolactin and its Receptor in Male Reproduction. Horm. Metab. Res. 2019, 51, 215–219. [Google Scholar] [CrossRef] [Green Version]
- Glenn, D.R.; McVicar, C.M.; McClure, N.; Lewis, S.E. Sildenafil citrate improves sperm motility but causes a premature acrosome reaction in vitro. Fertil. Steril. 2007, 87, 1064–1070. [Google Scholar] [CrossRef]
- Tardif, S.; Madamidola, O.A.; Brown, S.; Frame, L.; Lefièvre, L.; Wyatt, P.G.; Barratt, C.L.; da Silva, S.M. Clinically relevant enhancement of human sperm motility using compounds with reported phosphodiesterase inhibitor activity. Hum. Reprod. 2014, 29, 2123–2135. [Google Scholar] [CrossRef] [PubMed]
- WHO. Laboratory Manual for the Examination and Processing of Human Semen, 6th ed.; World Health Organization: Geneva, Switzerland, 2021. [Google Scholar]
- Christie, B. Doctors revise Declaration of Helsinki. BMJ 2000, 321, 913. [Google Scholar] [CrossRef] [Green Version]
- Mortimer, D. Practical Laboratory Andrology; Oxford University Press on Demand: Oxford, UK, 1994. [Google Scholar]
- Thomas, P.; Meizel, S. Phosphatidylinositol 4,5-bisphosphate hydrolysis in human sperm stimulated with follicular fluid or progesterone is dependent upon Ca2+ influx. Biochem. J. 1989, 264, 539–546. [Google Scholar] [CrossRef] [Green Version]
- Costa, L.; Mendes, M.; Ferriani, R.; Moura, M.; Reis, R.; De Sá, M.S. Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes. Braz. J. Med. Biol. Res. 2004, 37, 1747–1755. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wen, X.; Li, D.; Tozer, A.J.; Docherty, S.M.; Iles, R.K. Estradiol, progesterone, testosterone profiles in human follicular fluid and cultured granulosa cells from luteinized pre-ovulatory follicles. Reprod. Biol. Endocrinol. 2010, 8, 117. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Helm, G.; Owman, C.; Rosengren, E.; Sjöberg, N.-O. Regional and Cyclic Variations in Catecholamine Concentration of the Human Fallopian Tube. Biol. Reprod. 1982, 26, 553–558. [Google Scholar] [CrossRef] [PubMed]
- Fait, G.; Vered, Y.; Yogev, L.; Gamzu, R.; Lessing, J.B.; Paz, G.; Yavetz, H. High levels of catecholamines in human semen: A preliminary study. Andrologia 2001, 33, 347–350. [Google Scholar] [CrossRef] [PubMed]
- Shah, G.V.; Desai, R.B.; Sheth, A.R. Effect of Prolactin on Metabolism of Human Spermatozoa. Fertil. Steril. 1976, 27, 1292–1294. [Google Scholar] [CrossRef]
- Burkman, L.J. Characterization of Hyperactivated Motility by Human Spermatozoa During Capacitation: Comparison of Fertile and Oligozoospermic Sperm Populations. Arch. Androl. 1984, 13, 153–165. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fukuda, A.; Mori, C.; Hashimoto, H.; Noda, Y.; Mori, T.; Hoshino, K. Effects of prolactin during preincubation of mouse spermatozoa on fertilizing capacity in vitro. J. Assist. Reprod. Genet. 1989, 6, 92–97. [Google Scholar] [CrossRef]
- Rijnders, S.; Bolscher, J.G.M.; McDonnell, J.; Vermeiden, J.P.W. Filling Time of a Lamellar Capillary-Filling Semen Analysis Chamber Is a Rapid, Precise, and Accurate Method to Assess Viscosity of Seminal Plasma. J. Androl. 2007, 28, 461–465. [Google Scholar] [CrossRef] [Green Version]
- Van Der Horst, G.; Maree, L. SpermBlue®: A new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotech. Histochem. 2010, 84, 299–308. [Google Scholar] [CrossRef] [PubMed]
- Microptic Automatic Diagnostic Systems. Protocols 2020. Available online: https://www.micropticsl.com/documents-support/protocols/ (accessed on 31 January 2021).
- Mortimer, S.T. A critical review of the physiological importance and analysis of sperm movement in mammals. Hum. Reprod. Updat. 1997, 3, 403–439. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mortimer, D.; Mortimer, S.T. Computer-Aided Sperm Analysis (CASA) of sperm motility and hyperactivation. In Spermatogenesis; Humana Press: Totowa, NJ, USA, 2013; pp. 77–87. [Google Scholar] [CrossRef]
- VAN DER Horst, G.; Seier, J.V.; Spinks, A.C.; Hendricks, S. The maturation of sperm motility in the epididymis and vas deferens of the vervet monkey, Cercopithecus aethiops. Int. J. Androl. 1999, 22, 197–207. [Google Scholar] [CrossRef] [PubMed]
- Van der Horst, G.; Luther, I.; Goeritz, F.; Hermes, R.; Dierich, J.; Lueders, I.; Tordiff, A.; Bartels, P.; Potier, R.; Baker, B.; et al. Computer aided semen analysis of Loxodonta africana sperm. In Proceedings of the International Elephant Conservation and Research Symposium, Kwalata Game Lodge, Pretoria, South Africa, 25–29 January 2010; pp. 25–29. [Google Scholar]
- Rahman, S.; Kwon, W.-S.; Pang, M.-G. Calcium Influx and Male Fertility in the Context of the Sperm Proteome: An Update. BioMed Res. Int. 2014, 2014, 841615. [Google Scholar] [CrossRef]
- Mannowetz, N.; Miller, M.R.; Lishko, P.V. Regulation of the sperm calcium channel CatSper by endogenous steroids and plant triterpenoids. Proc. Natl. Acad. Sci. USA 2017, 114, 5743–5748. [Google Scholar] [CrossRef] [Green Version]
- Ghanbari, H.; Keshtgar, S.; Kazeroni, M. Inhibition of the CatSper Channel and NOX5 Enzyme Activity Affects the Functions of the Progesterone-Stimulated Human Sperm. Iran. J. Med. Sci. 2018, 43, 18–25. [Google Scholar] [CrossRef]
- Berendsen, J.T.W.; Kruit, S.A.; Atak, N.; Willink, E.; Segerink, L.I. Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa. Anal. Chem. 2020, 92, 3302–3306. [Google Scholar] [CrossRef]
- Contreras, H.R.; Llanos, M.N. Detection of progesterone receptors in human spermatozoa and their correlation with mor-phological and functional properties. Int. J. Androl. 2001, 24, 246–252. [Google Scholar] [CrossRef]
- Pujianto, D.A.; Zaini, M.; Salim, S.O. Progesterone Increases the Progressive Motility of Human Sperm. J. Int. Dent. 2019, 12, 228–231. [Google Scholar]
- Calogero, A.E.; Burrello, N.; Barone, N.; Palermo, I.; Grasso, U.; D’Agata, R. Effects of progesterone on sperm function: Mechanisms of action. Hum. Reprod. 2000, 15, 28–45. [Google Scholar] [CrossRef] [Green Version]
- Nowicka-Bauer, K.; Szymczak-Cendlak, M. Structure and Function of Ion Channels Regulating Sperm Motility—An Overview. Int. J. Mol. Sci. 2021, 22, 3259. [Google Scholar] [CrossRef]
- Alasmari, W.; Costello, S.; Correia, J.; Oxenham, S.K.; Morris, J.; Fernandes, L.; Ramalho-Santos, J.; Kirkman-Brown, J.C.; Michelangeli, F.; Publicover, S.; et al. Ca2+ Signals Generated by CatSper and Ca2+ Stores Regulate Different Behaviors in Human Sperm. J. Biol. Chem. 2013, 288, 6248–6258. [Google Scholar] [CrossRef] [Green Version]
- Tantibhedhyangkul, J.; Hawkins, K.C.; Dai, Q.; Mu, K.; Dunn, C.N.; Miller, S.E.; Price, T.M. Expression of a mitochondrial progesterone receptor in human spermatozoa correlates with a progestin-dependent increase in mitochondrial membrane potential. Andrology 2014, 2, 875–883. [Google Scholar] [CrossRef] [Green Version]
- Achikanu, C.; Pendekanti, V.; Teague, R.; Publicover, S. Effects of pH manipulation, CatSper stimulation and Ca2+-store mobilization on [Ca2+]i and behaviour of human sperm. Hum. Reprod. 2018, 33, 1802–1811. [Google Scholar] [CrossRef]
- Brown, S.G.; Publicover, S.J.; Barratt, C.; Da Silva, S.J.M. Human sperm ion channel (dys)function: Implications for fertilization. Hum. Reprod. Updat. 2019, 25, 758–776. [Google Scholar] [CrossRef] [PubMed]
- Denisenko, V.; Chistyakova, I.; Volkova, N.; Volkova, L.; Iolchiev, B.; Kuzmina, T. The Modulation of Functional Status of Bovine Spermatozoa by Progesterone. Animals 2021, 11, 1788. [Google Scholar] [CrossRef] [PubMed]
- Chung, J.-J.; Shim, S.-H.; Everley, R.A.; Gygi, S.P.; Zhuang, X.; Clapham, D.E. Structurally Distinct Ca2+ Signaling Domains of Sperm Flagella Orchestrate Tyrosine Phosphorylation and Motility. Cell 2014, 157, 808–822. [Google Scholar] [CrossRef] [Green Version]
- Fan, Y.P.; Tang, J.J.; Lu, H.; Zhang, Y.C.; Ruan, J.L.; Teng, X.M.; Han, Y.B. Progesterone induction keeps a balanced mito-chondrial activity and a low ROS productivity in human sperm. Zhonghua Nan Ke Xue 2013, 19, 880–885. [Google Scholar]
- Condorelli, R.A.; La Vignera, S.; Di Bari, F.; Unfer, V.; E Calogero, A. Effects of myoinositol on sperm mitochondrial function in-vitro. Eur. Rev. Med. Pharmacol. Sci. 2011, 15, 129–134. [Google Scholar]
- Palmieri, M.; Papale, P.; Della Ragione, A.; Quaranta, G.; Russo, G.; Russo, S. In VitroAntioxidant Treatment of Semen Samples in Assisted Reproductive Technology: Effects of Myo-Inositol on Nemaspermic Parameters. Int. J. Endocrinol. 2016, 2016, 2839041. [Google Scholar] [CrossRef] [Green Version]
- Qamar, A.Y.; Fang, X.; Kim, M.J.; Cho, J. Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm. Animals 2019, 9, 1038. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Governini, L.; Ponchia, R.; Artini, P.G.; Casarosa, E.; Marzi, I.; Capaldo, A.; Luddi, A.; Piomboni, P. Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment. J. Clin. Med. 2020, 9, 1638. [Google Scholar] [CrossRef]
- Artini, P.G.; Casarosa, E.; Carletti, E.; Monteleone, P.; Di Noia, A.; Di Berardino, O.M. In vitro effect of myo-inositol on sperm motility in normal and oligoasthenospermia patients undergoing in vitro fertilization. Gynecol. Endocrinol. 2016, 33, 109–112. [Google Scholar] [CrossRef]
- Dcunha, R.; Hussein, R.S.; Ananda, H.; Kumari, S.; Adiga, S.K.; Kannan, N.; Zhao, Y.; Kalthur, G. Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction. Reprod. Sci. 2020, 1–19. [Google Scholar] [CrossRef]
- Korosi, T.; Barta, C.; Rokob, K.; Torok, T. Physiological Intra-Cytoplasmic Sperm Injection (PICSI) outcomes after oral pre-treatment and semen incubation with myo-inositol in oligoasthenoteratozoospermic men: Results from a prospective, ran-domized controlled trial. Eur Rev. Med. Pharmacol Sci. 2017, 21, 66–72. [Google Scholar]
- De Luca, M.N.; Colone, M.; Gambioli, R.; Stringaro, A.; Unfer, V. Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols. Antioxidants 2021, 10, 1283. [Google Scholar] [CrossRef]
- Cariati, F.; Galdiero, G.; Coppola, G.; Galdiero, M.; Salzano, C.; Pivonello, C.; Patalano, R.; Alviggi, C.; De, P.G.; Colao, A.; et al. The role of dopamine pathway on human sperm: In vitro effect of dopamine receptor agonists and antagonists on sperm motility, kinetics and viability. In Proceedings of the 18th European Congress of Endocrinology, Munich, Germany, 28–31 May 2016; Volume 41. [Google Scholar] [CrossRef] [Green Version]
- Singh, A.P.; Sarkar, S.; Tripathi, M.; Rajender, S. Mucuna pruriens and Its Major Constituent L-DOPA Recover Spermatogenic Loss by Combating ROS, Loss of Mitochondrial Membrane Potential and Apoptosis. PLoS ONE 2013, 8, e54655. [Google Scholar] [CrossRef] [Green Version]
- Chan, C.-C.; Shui, H.-A.; Wu, C.-H.; Wang, C.-Y.; Sun, G.-H.; Chen, H.-M.; Wu, G.-J. Motility and Protein Phosphorylation in Healthy and Asthenozoospermic Sperm. J. Proteome Res. 2009, 8, 5382–5386. [Google Scholar] [CrossRef]
- Way, A.L.; Killian, G.J. Capacitation and induction of the acrosome reaction in bull spermatozoa with norepinephrine. J. Androl. 2002, 23, 352–357. [Google Scholar]
- Bibov, M.Y.; Kuzmin, A.V.; Alexandrova, A.A.; Chistyakov, V.A.; Dobaeva, N.M.; Kundupyan, O.L. Role of the reactive oxygen species induced DNA damage in human spermatozoa dysfunction. AME Med. J. 2018, 3, 19. [Google Scholar] [CrossRef]
- Gonzales, G.; Velasquez, G.; Garcia-Hjarles, M. Hypoprolactinemia as Related to Seminal Quality and Serum Testosterone. Arch. Androl. 1989, 23, 259–265. [Google Scholar] [CrossRef]
- Parte, P.P.; Rao, P.; Redij, S.; Lobo, V.; D’Souza, S.J.; Gajbhiye, R.; Kulkarni, V. Sperm phosphoproteome profiling by ultra performance liquid chromatography followed by data independent analysis (LC–MSE) reveals altered proteomic signatures in asthenozoospermia. J. Proteom. 2012, 75, 5861–5871. [Google Scholar] [CrossRef]
- Binart, N.; Melaine, N.; Pineau, C.; Kercret, H.; Touzalin, A.M.; Imbert-Bolloré, P.; Kelly, P.A.; Jegou, B. Male Reproductive Function Is Not Affected in Prolactin Receptor-Deficient Mice. Endocrinology 2003, 144, 3779–3782. [Google Scholar] [CrossRef] [Green Version]
- Stovall, D.W.; Shabanowitz, R.B. The effects of prolactin on human sperm capacitation and acrosome reaction. Fertil. Steril. 1991, 56, 960–966. [Google Scholar] [CrossRef]
Mean ± SD | 95% C.I | |
---|---|---|
Total Mot (%) | 54.5 ± 18.5 | 51.7–57.3 |
Prog Mot (%) | 27.3 ± 16.5 | 24.8–29.8 |
MPT (106/ejaculate) | 28.3 ± 27.0 | 24.2–32.4 |
pH | 7.4 ± 0.2 | 7.4–7.5 |
Viscosity (cP) | 10.8 ± 11.1 | 9.1–12.5 |
Volume (mL) | 3.2 ± 2.9 | 2.8–3.7 |
Conc (106/mL) | 58.6 ± 42.8 | 52.2–65.1 |
Conc (106/ejaculate) | 161.3 ± 143.5 | 139.6–183.0 |
Vitality (%) | 69.9 ± 10.7 | 68.3–71.6 |
Normal (%) | 5.7 ± 4.6 | 5.0–6.4 |
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Keyser, S.; van der Horst, G.; Maree, L. Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations. Life 2021, 11, 1250. https://doi.org/10.3390/life11111250
Keyser S, van der Horst G, Maree L. Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations. Life. 2021; 11(11):1250. https://doi.org/10.3390/life11111250
Chicago/Turabian StyleKeyser, Shannen, Gerhard van der Horst, and Liana Maree. 2021. "Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations" Life 11, no. 11: 1250. https://doi.org/10.3390/life11111250