1. Introduction
Ramie (
Boehmeria nivea L.) is a perennial fiber yielding crop, which generally requires high inputs to achieve potential yield and maintain a good fiber quality [
1]. The nutrient requirements for ramie production are generally two to four times higher than that of the normal field crops. For example, the recommended demands of N, P
2O
5, and K
2O for the production of 100 kg ramie fiber are 8.00 kg, 2.00 kg, and 9.00 kg [
2], respectively, while they are only 2.56 kg, 0.77 kg, and 2.53 kg for the production of 100 kg corn grains [
3]. Actually, during the farmer’s production process, higher amounts of fertilizer are usually applied as most Chinese farmers believe the higher the fertilizer application, the higher the crop yield. Over-fertilization exists in almost all the agricultural production fields in China, which results in the crops’ nutrient uptake efficiencies being generally lower than 30% or even 20% [
4]. This over-fertilization has caused a series of problems such as surface and groundwater pollution, as well as increasing greenhouse gas emissions. To address these problems, the Chinese government has implemented a strategy of “agricultural transformation and upgrading”. One important task of the strategy is to establish a sustainable agricultural production system with special emphasis on low input cropping systems.
When implementing such a system in ramie production, the key aspect is to minimize the use of production inputs, especially use of synthetic fertilizers [
5]. One way to reduce fertilizer input is through optimization of the cultivation techniques [
2]. However, it is still advised to breed new ramie varieties that are characterized by high NUE (nutrient utilization efficiency). Traditionally, plant breeding was conducted by altering the plant’s own genomic information. Recently, a new perspective of “holobiont” breeding has emerged and been accepted [
6], which claims “plant breeding goes microbial” [
7]. The holobiont considerers the plant and its associated microbiome as an evolutionary unit, which together transmit the genetic information to next generation [
8]. Therefore, future breeding should set the selection targets not only in terms of plant materials, but also for the associated microbes.
Soil microorganisms play a vital role in nutrient recycling [
9]. During our previous research, the helpful roles of soil microbes in the adaptation of the ramie to poor soil conditions were confirmed [
10]. This is generally achieved through enrichment of the beneficial bacteria and through a reduction of harmful fungi simultaneously. The current results indicate that ramie’s NUE can be improved through holobiont breeding. However, the premise for achieving this potential lies in understanding how the microorganisms improve the ramie’s NUE.
Therefore, the current study is designed with an overall aim to explore the role of soil microbes for improving the NUE of the ramie plant. The improvement in NUE by microorganisms is mainly evaluated through their potential for increasing the amount of nutrients available for plant uptake and subsequent utilization [
9]. For example, in legume species, the high NUE is attributed to the N-fixing characteristic, which is also true for some non-legume species such as miscanthus [
11,
12]. Phosphate solubilizing microorganisms (PSM) can convert immobilized inorganic P to soluble organic P (i.e., P-solubilization), which increases the bioavailable P for plant uptake and utilization [
13]. Our previous work already detected the N-fixing bacteria of
Bradyrhizobium from the rhizosphere soil of ramie using sequencing technology [
10]. However, the exact N-fixing potential is not clear. Therefore, the first objective (Objective I) of this study is to quantitatively assess the N-fixing potential of the ramie rhizosphere soil and to evaluate the contribution of the N-fixing characteristic in the poor soil tolerance of the ramie plant. Next to nitrogen, phosphorus is the second most important element for plant growth. Thus, the second objective (Objective II) of this study is to explore the role of P-solubilization, which is similar to N-fixing, and its potential contribution towards adaptation of the ramie plant to poor soil conditions. Another key aspect is the role of soil enzymes, which act as a bridge between the microorganism and N-fixing/P-solubilization [
14]. To explore how the microorganisms affect the potential of N-fixing/P-solubilization (Objective III), the activities of the nutrient-related soil enzymes are also monitored and compared as a part of this study. Furthermore, based on the outcomes of the former research work [
10], it is revealed that the harmful fungal communities (e.g.,
Cladosporium) can be enriched in the ramie rhizosphere soil, which can kill the beneficial bacteria and limit the N-fixing potential. Therefore, the fourth objective (Objective IV) is to verify the inhibitory effect of fungal communities on N-fixing bacterial communities in the rhizosphere.
2. Materials and Methods
The N-fixing and P-solubilization potential of soils derived from the rhizosphere of several ramie genotypes have different tolerance levels to poor soil conditions. The tolerance level is expressed by the overall plant field performance under poor soil conditions, as a higher field performance will have a stronger poor soil tolerance ability. The overall field performance was normalized (detail calculation process shown in [
10]) and then expressed to a field performance index (NFPI). The N-fixing potential was quantified by the number of nitrogen-fixing bacteria in the soil and the related nitrogenase activity. The P-solubilization quantity was measured as the available phosphorus (AP) content in the TCP (tricalcium phosphate) liquid medium after microbial cultivation. Other than nitrogenase, the activity of acid phosphatase (S-ACP), urease (S-UE), and sucrase (S-SC) were also tested and compared in this study. Their contributions for helping the ramie plant adapt to poor soil conditions were evaluated through the correlation analysis between the soil enzyme activity and the corresponding NFPI value. Quantification of the anti-N-fixing-bacteria activity by fungal communities was conducted using the inhibition-zone assay.
2.1. Materials and Sampling Strategies
All of the above-described tests were conducted using the fresh soil samples collected in December 2020. Soil samples of four ramie genotypes and one blank control CK (i.e., without ramie cultivation) were compared. The four ramie genotypes, namely Xiangzhu XB (XZ-XB), Zhongzhu 1 (ZZ-1), Xiangzhu X2 (XZ-X2), and Xiangzhu 3 (XZ-3), were established in 2010 at the experimental station of Huarong (29°32′46″ N, 112°39′57″ E, 73 m a.s.l.). The CK was set as the weed free bare ground, which was adjacent to the ramie cultivation block. Without ramie cultivation, the CK block was dominated by the species of
Chrysanthemum indicum L. and
Humulus scandens (Lour.) Merr. The field soil is a poor sandy red soil that was found to have a total nitrogen content of 0.69 g/kg, available phosphorus content of 9.62 mg/kg, and exchangeable potassium content of 56.53 mg/kg. According to the field evaluation results [
10], the poor soil tolerance ability of the four tested genotypes were shown as XZ-XB (
NFPI = 0.953) > ZZ-1(
NFPI = 0.701) > XZ-X2(
NFPI = 0.452) > XZ-3 (
NFPI = 0.000). More detailed information about the experiment design and the field performance of the genotypes can be found in our previously published work [
10]. The rhizosphere soil samples of the ramie plants were collected in the field and transported to the laboratory in an ice box. The rhizosphere soil samples, defined as the soil remaining attached to the roots after shaking plants vigorously [
15], were collected according to the “air shaking method”. The rhizosphere soil samples of five plants within one block were randomly selected in an “S” pattern and then mixed to one composite rhizosphere sample. Five soil cores (0–20-cm depth) within the CK block were collected according to the “S” pattern and were mixed to be the composite CK sample. All the samples were sieved through 50-mesh sieves and stored at 4 °C until the start of the test (less than 30 days here).
2.2. Quantification of the Number of Soil N-Fixing Bacteria
The number of the N-fixing bacteria was quantified using the spread plate method, with a detailed procedure comprised of following steps:
(a) Preparation of the soil suspension by adding 10.00 g of the fresh soil sample to 90 mL sterile distilled water and then shaking at 30 °C at 150 rpm for 30 min. (b) Preparation of the diluted soil suspension by 10-, 102-, 103-, 104-, and 105-fold dilutions of the suspension, which was prepared by adding 1 mL previous-level-fold suspension to 9 mL of sterile distilled water. (c) Determination of the N-fixing bacteria number of 103-, 104-, and 105-fold diluted suspensions were plated onto Petri plates containing Ashby nitrogen-free solid medium. This was followed by 3 d incubation at 30 °C and the number of N-fixing bacteria in each plate was counted. Afterwards, the counted number was converted to the comparable number, expressed as log c.f.u. per gram of dry soil.
The above steps of each soil sample were repeated four times. The composition of the Ashby nitrogen-free solid medium included 10.00 g mannitol, 0.20 g KH2PO4, 0.20 g MgSO4, 0.20 g NaCl, 0.30 g K2SO4, 5.0 g CaCO3, 1000.00 mL distilled water, and 18.00 g agar. At first, these components were mixed and adjusted to a pH of 7.0, followed by sterilization at 121 °C for 30 min.
2.3. Quantitative Assay to Determine the P Solubilization Efficiency of Rhizosphere Microbes
In this assay, each 250 mL Erlenmeyer flasks containing 90.00 mL TCP liquid medium was inoculated with 10.00 mL soil suspension (see
Step A in
Section 2.2) and then shook at 30 °C at 150 rpm for 72 h. To eliminate the background effect, a control treatment was carried out simultaneously without the soil suspension and adding only the corresponding sterilized (at 121 °C for 30 min) suspension. At the end of incubation time, 5 mL cultures were sampled and centrifuged at 12,000 rpm for 5 min. Afterwards, the supernatants were used to determine the AP contents by the molybdenum blue method. The net P-solubilization quantity of each sample was the difference of AP content between the normal treatment and control treatment. Each soil sample had four replications. The composition of the TCP liquid medium included 0.30 g NaCl, 0.30 g MgSO
4·7H
2O, 0.50 g (NH
4)
2SO
4, 0.30 g KCl, 0.03 g FeSO
4·7H
2O, 0.03 g MnSO
4·4H
2O, 5.00 g Ca
3(PO
4)
2, 10.00 g glucose, and 1000.00 mL distilled water. In addition, prior to microbial cultivation, the pH of the aforementioned medium was adjusted to 7.0 and sterilized at 121 °C for 30 min.
2.4. Quantitative Estimation of the Soil Enzyme Activity
The nitrogenase activity was measured using an acetylene reduction assay (ARA) and was expressed by the conversion efficiency of acetylene (C
2H
2) to ethylene (C
2H
4). The detailed description of ARA method is as follows: (1) adding 0.50 mL soil suspension to each serum bottle (100 mL) containing 50.00 mL nitrogen-free Ashby liquid medium; (2) sealing the bottle by cotton plugs and incubation at 30 °C and 150 rpm for 72 h; (3) replacing cotton plugs with air tight serum stopper; (4) removing 5.00 mL atmospheric air from the tube and injecting same volume of acetylene; and (5) afterwards, continue with incubation for another 72 h and draw 1 mL gas sample from the tube to measure the C
2H
4 concentration using gas chromatography. Based on this measured concentration, the amount of C
2H
4 produced per gram of dry soil per 24 h is the nitrogenase activity, which was calculated according to the method described in the study of Haskett [
16].
The activities of S-ACP, S-UE, and S-SC were determined using the Solarbio detection kits (Solarbio Technology Co., Ltd., Beijing, China) of BC0140, BC0120, and BC0240, respectively, as per the manufacturer’s instructions. The S-UE activity was expressed as the amount of NH3-N, the S-ACP activity as the amount of phenol, and the S-SC activity as the amount of reducing sugar produced per gram of dry soil after 24 h at 37 °C.
2.5. Antagonistic Test to Quantify the Effect of Fungal Communities on N-Fixing Bacteria
Quantification of the anti-N-fixing-bacteria activity by fungal communities was conducted using an inhibition-zone assay. In this assay, the dot culture of the fungal colony on the N-fixing bacteria grown in Petri plates was carried out. The inhibition efficiency was calculated as halo zone diameter/colony diameter (HD/CD). The procedure comprised the following: (a) enrichment of the N-fixing bacteria by adding 5.00 mL soil suspension to 95.00 mL sterilized liquid N-fixing bacteria enrichment medium (15.00 g glucose, 0.80 g KH2PO4, 0.20 g MgSO4, 0.20 g NaCl, 1.00 g CaCO3, 1.00 mL Na2MoO4 (mass fraction of 1%), 1.00 mL H3BO3 (mass fraction of 1%), 1.00 mL MnSO4 (mass fraction of 1%), 1.00 mL FeSO4·7H2O (mass fraction of 1%) and 1000.00 mL distilled water) and then incubated at 30 °C and 150 rpm for 3 days, then taking 5.00 mL of the bacterial culture and enriching again by following the same procedure. (b) Preparation of the N-fixing bacteria to be cultured in petri plates by plating 1.00 mL dule-enriched N-fixing bacterial culture to the surface of petri plates containing a solid LB (Luria-Bertani) medium followed by incubation at 30 °C for 3 days. (c) Preparation of the fungal colony by plating 1 mL ten-fold diluted (10−1) soil suspension on the surface of Petri plates containing a solid PAD medium, followed by incubation at 28 °C until the whole surface was covered by mycelium. Afterwards, a fungal colony was cut using a 5 mm cork borer. (d) Co-incubation of the fungal colony and N-fixing bacteria in Petri plates by spotting three fungal colonies and one control colony (prepared according to the same method for fungal colony with replacing 1 mL soil suspension to 1 mL distilled water) on the surface of N-fixing bacteria grown at four equidistant points near the Petri center in four directions, followed by co-incubation of the paired plates at 30 °C for 3 days. (e) At the end of incubation, the diameter of the halo and the colony were measured.
All of the above steps for each sample were carried out in four replications.
2.6. Statistical Analysis
Data are presented as mean ± SD. Differences in terms of the tested parameters as described above among the different ramie genotypes were analyzed using one-way ANOVA (analysis of variance) in SAS 9.4 software (SAS Institute, Cary, NC, USA). The mean values of these parameters were compared using Duncan’s multiple range tests at both a p < 0.05 and p < 0.01 level. If 0.01 < p < 0.05, the significance was marked by p < 0.05, otherwise by p < 0.01. A correlation analysis was performed between the activities of the four tested soil enzymes and the poor soil tolerance ability of ramie plants (expressed by the NFPI).
4. Discussion
Historically, research in terms of N-fixing microbes was only conducted on legume species due to the specific characteristics of the rhizobia. However, recently, an increasing number of studies have also shown the existence of N-fixing bacteria on non-legume species, such as corn [
17], miscanthus [
12], and sugarcane [
18]. N-fixing bacteria can reduce N
2 in the air to a form (mainly NH
4+) that can be absorbed and utilized by plants, especially under the N-deficiency condition [
19]. This explains the positive correlation between the poor soil tolerance ability of the ramie plant and the N-fixing efficiency of the rhizosphere microbes. The N-fixing efficiency is co-contributed by the N-fixing bacteria number and nitrogenase activity. The different N-fixing bacteria number between genotypes can be explained by the plant that controls the N-fixing bacteria number by controlling the type and amount of root exudates [
20,
21]. Besides, the results of present study indicate the harmful fungal communities is also a factor in determining the N-fixing bacteria number. Additionally, the N-fixing efficiency is more related with the nitrogenase activity. Wang [
22] found bacterial strains with nearly 10-times difference of nitrogenase activity in potato rhizosphere soil. Although the N-fixing potential is confirmed by ramie, there is still a large gap compared with legume species. For example, the highest N-fixing potential in this study is only 10–20% of that by soybean [
23]. In the future, more studies are required to close the gap. Moreover, this paper only generally describes the existence of N-fixing bacteria in the ramie rhizosphere soil and its positive contribution in help ramie plant adapting to the poor soil condition. To apply this characteristic in future holobiont breeding, it is necessary to identify the specific species of the N-fixing bacteria and then create a stable genetic holobiont group of ramie plant-nitrogen fixing bacteria. For nitrogen cycling, urease also plays an important role in affecting the hydrolysis process of urea [
24]. However, there is no significant difference in the urease activity among the tested genotypes, indicating that ramie plants did not adapt to a poor soil environment by affecting the utilization of urea.
This study also found that the P-solubilization by soil microorganisms makes a positive contribution to the adaptation of the ramie to poor soil. The soil microorganisms can secrete organic acids, protons, polysaccharides, and other substances. These substances could accelerate the conversion of insoluble P (e.g., rock P) to soluble form, which prevents the phosphorus availability and absorption by plants [
25,
26]. This explains the positive role of soil microorganisms in promoting the growth of ramie under poor soil conditions. The P-solubilization efficiency of the microorganism is mainly controlled by the generated phosphatase activity [
27]. In this paper, the P-solubilization efficiency of the microorganism is consistent with the corresponding acid phosphatase activity, which also proves the applicability of this view in ramie plants. This study also finds that although there are significant differences in sucrase activity among different ramie genotypes, it is not significantly correlated with the adaptability of the ramie to poor soil. As an important material for catalyzing the decomposition of organic matter, the sucrase activity is closely related to the soil fertility [
28]. However, the soil fertility is mainly increased in terms of organic matter, but not in the mineral elements such as N, P, and K.
The results of this study confirm the N-fixing and P-solubilization potential of the ramie rhizosphere soil microbes, especially from the poor soil tolerable genotype. In addition, the negative effects of the rhizosphere soil fungal community on the N-fixing bacterial are confirmed. These results are summarized based on the artificial experiments that were conducted in the sterilized conditions with only one or a few microbial strains. However, in reality, soil microbes grown in an unsterilized condition encounter a more diverse microbial community. The diverse condition suggests a more serious interaction potential between different microbes [
29]. This could weaken or also strengthen the microbe’s effect tested in the artificial conditions. For this reason, a more realistic test of the N-fixing and P-solubilization potential of the ramie rhizosphere soil microbes is required. This can be conducted by isolating the N-fixing, P-solubilization microbes firstly, then adding the isolation inoculum to unsterilized soil and evaluating their potential on the growth and NUE improvement of the ramie plant. In future isolation research, the effect of the cultivation medium should be taken into consideration to get high selectivity and reliability. In this study, the Ashby medium was used in the N-fixing cultivation as it is one of the most common and suitable media for diazotrophs co-cultivation [
30,
31,
32]. Actually, diazotroph is not only one kind of prokaryote, but includes several different kinds such as
Rhizobium,
Ensifer,
Azospirillum [
33,
34]. Each kind of diazotroph has its own most suitable media as, in general, Ashby for
Azotobacter [
35] and yeast extract mannitol agar (YMA) for
Rhizobium [
31]. The exact species of the ramie rhizosphere contented N-fixing bacteria are still not confirmed. To isolate the N-fixing bacteria more effectively in the future study, different cultivation mediums, e.g.,
Beijerinckia medium and
Derxia medium, should be compared.