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Reference Intervals for Acetylated Fetal Hemoglobin in Healthy Newborns

Renata Paleari
Irene Mutta
Gianluca Musolino
Alessandro Salvatoni
Massimo Agosti
Gaia Francescato
3 and
Andrea Mosca
Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Centro per la Riferibilità Metrologica in Medicina di Laboratorio (CIRME), Università degli Studi di Milano, Via Fratelli Cervi 93, 20090 Segrate, Italy
Unità Operativa di Endocrinologia, Diabetologia e Pediatria d’Urgenza, Clinica Pediatrica Università degli Studi dell’Insubria, Varese, Italy
Unità Operativa Complessa di Neonatologia e Terapia Intensiva Neonatale, Ospedale F. Del Ponte, Varese, Italy
Author to whom correspondence should be addressed.
Thalass. Rep. 2014, 4(2), 2120;
Submission received: 18 November 2013 / Revised: 9 May 2014 / Accepted: 9 May 2014 / Published: 29 September 2014


The acetylated fetal hemoglobin (AcHbF) derives from an enzyme-mediated post-translational modification occurring on the N-terminal glycine residues of γ-chains. At present, no established data are available on reference intervals for AcHbF in newborns. A total of 92 healthy infants, with gestational age between 37 and 41 weeks were selected for the establishment of AcHbF reference intervals. Blood samples were collected by heel pricking, when collecting routine neonatal screening, and the hemoglobin pattern was analyzed by high-performance liquid chromatography. AcHbF results were then normalized for HbF content in order to account for differences in hemoglobin switch. No difference was found in AcHbF values between genders (P = 0.858). AcHbF results were as follow: 12.8 ± 0.8% (mean±standard deviation), reference interval: 11.3–14.3%. This finding could facilitate further studies aimed to assess the possible use of AcHbF, for instance as a possible fetal metabolic biomarker during pregnancy.
Keywords: fetal hemoglobin; HbA1c; neonatal screening fetal hemoglobin; HbA1c; neonatal screening

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MDPI and ACS Style

Paleari, R.; Mutta, I.; Musolino, G.; Salvatoni, A.; Agosti, M.; Francescato, G.; Mosca, A. Reference Intervals for Acetylated Fetal Hemoglobin in Healthy Newborns. Thalass. Rep. 2014, 4, 2120.

AMA Style

Paleari R, Mutta I, Musolino G, Salvatoni A, Agosti M, Francescato G, Mosca A. Reference Intervals for Acetylated Fetal Hemoglobin in Healthy Newborns. Thalassemia Reports. 2014; 4(2):2120.

Chicago/Turabian Style

Paleari, Renata, Irene Mutta, Gianluca Musolino, Alessandro Salvatoni, Massimo Agosti, Gaia Francescato, and Andrea Mosca. 2014. "Reference Intervals for Acetylated Fetal Hemoglobin in Healthy Newborns" Thalassemia Reports 4, no. 2: 2120.

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