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Article

Cultural Characteristics and Antibiotic Susceptibility Pattern of Helicobacter pylori Isolated from Dyspepsia Patients

by
Bolanle A. Adeniyi
1,*,
Temitope O. Lawal
1,
Jesse A. Otegbayo
2,
Olayiwola A. Oluwasola
3,
Georgina N. Odaibo
4,
Samuel O. Ola
2,
Clement A. Okolo
3,
Adegboyega Akere
2 and
Aderemi O. Kehinde
5
1
Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria
2
Department of Medicine, University of Ibadan, Nigeria
3
Department of Pathology, University of Ibadan, Nigeria
4
Department of Virology, University of Ibadan, Nigeria
5
Department of Medical Microbiology, University of Ibadan, Nigeria
*
Author to whom correspondence should be addressed.
Gastroenterol. Insights 2012, 4(2), e21; https://doi.org/10.4081/gi.2012.e21
Submission received: 24 January 2012 / Revised: 13 May 2012 / Accepted: 10 July 2012 / Published: 19 November 2012

Abstract

Helicobacter pylori consist in a helical shaped Gram-negative bacterium, approximately 3 micrometers long with a diameter of approximately 0.5 micrometers. It has 4-6 flagella. It is microaerophilic and tests positive for oxidase, catalase and urease. With its flagella, the bacterium moves through the stomach lumen and drills into the mucus gel layer of the stomach. In humans, H. pylori have been associated with peptic ulcers, chronic gastritis, duodenitis and stomach cancer. It is widely believed that in the absence of treatment, H. pylori infection, once established in its gastric niche, persists for life. The aim of this research is to study the cultural characteristics and antibiotic susceptibility pattern of H. pylori strains isolated from southwest Nigeria. The cultural characteristics and antibiotic susceptibility pattern of Helicobacter pylori strains isolated from gastric mucosal antral biopsy specimens collected from 43 of 52 dyspepsia patients in the University College Hospital Ibadan, Oyo State, Nigeria, were determined using standard microbiological methods for Helicobacter pylori isolation. The 43 isolates were subjected to 23 different antibiotics and each of the antibiotics demonstrated a variable degree of activity against the isolates. Among the antibiotics to which the organism was most susceptible are: ofloxacin (30 μg) 100% activity, ciprofloxacin (5 μg) 97.67% activity, gentamicin (120 μg) 95.35 activity, amikacin (30 μg), kanamycin (30 μg) and chloramphenicol (30 μg) each 90.70% activity, clarithromycin (15 μg) 93.02, while the less active antibiotics are: augmentin (30 μg) 23.26% active, amoxycillin (25 μg) and metronidazole (50 μg) each 27.91% active and clindamycin (2 mg) 30.23% active. From the result of the antibiotic susceptibility pattern of the strains of the organism, 95.35% of the total isolates are multi drug resistant. Resistance was developed to, among others, augmentin (30 μg), amoxycillin (25 μg), metronidazole (50 μg) and clindamycin (2 mg).
Keywords: Helicobacter pylori; cultural characteristics; antibiotics susceptibility pattern; multi drug resistance Helicobacter pylori; cultural characteristics; antibiotics susceptibility pattern; multi drug resistance

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MDPI and ACS Style

Adeniyi, B.A.; Lawal, T.O.; Otegbayo, J.A.; Oluwasola, O.A.; Odaibo, G.N.; Ola, S.O.; Okolo, C.A.; Akere, A.; Kehinde, A.O. Cultural Characteristics and Antibiotic Susceptibility Pattern of Helicobacter pylori Isolated from Dyspepsia Patients. Gastroenterol. Insights 2012, 4, e21. https://doi.org/10.4081/gi.2012.e21

AMA Style

Adeniyi BA, Lawal TO, Otegbayo JA, Oluwasola OA, Odaibo GN, Ola SO, Okolo CA, Akere A, Kehinde AO. Cultural Characteristics and Antibiotic Susceptibility Pattern of Helicobacter pylori Isolated from Dyspepsia Patients. Gastroenterology Insights. 2012; 4(2):e21. https://doi.org/10.4081/gi.2012.e21

Chicago/Turabian Style

Adeniyi, Bolanle A., Temitope O. Lawal, Jesse A. Otegbayo, Olayiwola A. Oluwasola, Georgina N. Odaibo, Samuel O. Ola, Clement A. Okolo, Adegboyega Akere, and Aderemi O. Kehinde. 2012. "Cultural Characteristics and Antibiotic Susceptibility Pattern of Helicobacter pylori Isolated from Dyspepsia Patients" Gastroenterology Insights 4, no. 2: e21. https://doi.org/10.4081/gi.2012.e21

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