The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Electrospinning Solution
2.3. Preparation of Monoaxial Fibers
2.4. Scanning Electron Microscopy (SEM)
2.5. Fourier-Transform Infrared Spectroscopy (FTIR)
2.6. X-Ray Diffraction (XRD)
2.7. Ultraviolet/Visible Analysis (UV/Vis)
2.8. Drug Loading (DL), Entrapment Efficiency (EE%), and Fiber Yield (Y)
2.9. Drug Release Profile
2.10. Bacterial Suspensions Preparation for Microbiological Testing
2.11. The Minimum Inhibitory Concentration (MIC)
2.12. Antibacterial Zone of Inhibition Assay
2.13. Statistical Analysis
3. Results & Discussion
3.1. Morphological Characteristics of the Electrospun Fibers
3.2. Fourier-Transform Infrared Spectroscopy (FTIR)
3.3. X-Ray Diffraction (XRD)
3.4. UV Standard Curve
3.5. Drug Loading (DL), Encapsulation Efficiency (EE%), and Fiber Yield (Y)
3.6. Drug Release Profile
3.7. Antibacterial Activity
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Bacterial Strain | Zones of Inhibition (mm) | ||
---|---|---|---|
Halicin Disc | Blank Fibers | Drug-Loaded Fibers | |
E. coli (ATCC 22925) | 20 ± 0 | 0 | 27 ± 3 |
S. aureus (ATCC 29213) | 28 ± 1 | 0 | 21 ± 1 |
S. aureus (ATCC BAA 977) | 29 ± 1 | 0 | 30 ± 2 |
A. baumannii (BAA 747) | 25 ± 1 | 0 | 21 ± 2 |
A. baumannii (MDR 3086) | 22 ± 1 | 0 | 18 ± 1 |
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Aburayan, W.S.; Booq, R.Y.; BinSaleh, N.S.; Alfassam, H.A.; Bakr, A.A.; Bukhary, H.A.; Alyamani, E.J.; Tawfik, E.A. The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria. Pharmaceutics 2020, 12, 1189. https://doi.org/10.3390/pharmaceutics12121189
Aburayan WS, Booq RY, BinSaleh NS, Alfassam HA, Bakr AA, Bukhary HA, Alyamani EJ, Tawfik EA. The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria. Pharmaceutics. 2020; 12(12):1189. https://doi.org/10.3390/pharmaceutics12121189
Chicago/Turabian StyleAburayan, Walaa S., Rayan Y. Booq, Nouf S. BinSaleh, Haya A. Alfassam, Abrar A. Bakr, Haitham A. Bukhary, Essam J. Alyamani, and Essam A. Tawfik. 2020. "The Delivery of the Novel Drug ‘Halicin’ Using Electrospun Fibers for the Treatment of Pressure Ulcer against Pathogenic Bacteria" Pharmaceutics 12, no. 12: 1189. https://doi.org/10.3390/pharmaceutics12121189