Photocatalytic Hydrogen Production and Carbon Dioxide Reduction Catalyzed by an Artificial Cobalt Hemoprotein
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of Co(III)Mal-PPIXMME 4
2.2. Biohybrid Preparation
2.3. UV–Visible Characteristics of the (A3A3′)Y26C-4 Biohybrid
2.4. Computational Modeling Studies
2.5. Photocatalytic Activity of the (A3A3′)Y26C-4 Biohybrid
2.5.1. Light-Driven H+ Reduction
2.5.2. Light-Driven CO2 Reduction
3. Materials and Methods
3.1. Synthesis of Co(III)Mal-PPIX 4
3.1.1. Protoporphyrin IX Dimethyl Ester (PPIX DME) Hydrolysis [23]
3.1.2. Synthesis of Maleimido-Ethylamido-Protoporphyrin IX Monomethyl Ester Mal-PPIX MME 3
3.1.3. Insertion of Cobalt into Maleimido-Ethylamido-Protoporphyrin IX Monomethyl Ester Mal-PPIX MME
3.2. Production and Purification of αRep Proteins
3.2.1. Protein Production
3.2.2. Protein Purification
3.3. Covalent Anchoring of Compound 4 into (A3A3′)Y26C
3.4. Photocatalysis Experiments
3.5. Spectroscopic Characterization of the (A3A3′)Y26C-4 Biohybrid
3.6. Spectroscopic Characterization of the (A3A3′)Y26C-4 Biohybrid
3.7. Computational Methods
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Co(III) λMax (nm) | Co(II) λMax (nm) | |||||||
---|---|---|---|---|---|---|---|---|
Protein | Ligands | Soret | β | α | Ligand | Soret | α/β | Ref. |
Mb | His/H2O | 424 | 535 | 572 | His | 406 | 558 | [24] |
MbH64VV68A | His/H2O | 428 | 540 | 572 | His | 406 | 555 | [25] |
P450Cam | Cys/H2O | 422 | 538 | 570 | Cys | 404 | 556 | [26] |
CYP119 | Cys/H2O | 422 | 539 | 566 | Cys | 410 | 558 | [27] |
Oxd | His/H2O | 429 | 542 | 574 | His | 402 | 559 | [28] |
HRP | His/H2O | 421 | 533 | 565 | His | 401 | 553 | [24] |
DYP | His/H2O | 422 | 534 | 567 | His | 403 | 564 | [28] |
Cat | His/? | 427 | 537 | 570 | His | 403 | 561 | [28] |
(A3A3′)Y26C-4 | ? | 423 | 535 | 569 | ? | 399 | 562 | This work |
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Udry, G.A.O.; Tiessler-Sala, L.; Pugliese, E.; Urvoas, A.; Halime, Z.; Maréchal, J.-D.; Mahy, J.-P.; Ricoux, R. Photocatalytic Hydrogen Production and Carbon Dioxide Reduction Catalyzed by an Artificial Cobalt Hemoprotein. Int. J. Mol. Sci. 2022, 23, 14640. https://doi.org/10.3390/ijms232314640
Udry GAO, Tiessler-Sala L, Pugliese E, Urvoas A, Halime Z, Maréchal J-D, Mahy J-P, Ricoux R. Photocatalytic Hydrogen Production and Carbon Dioxide Reduction Catalyzed by an Artificial Cobalt Hemoprotein. International Journal of Molecular Sciences. 2022; 23(23):14640. https://doi.org/10.3390/ijms232314640
Chicago/Turabian StyleUdry, Guillermo A. Oliveira, Laura Tiessler-Sala, Eva Pugliese, Agathe Urvoas, Zakaria Halime, Jean-Didier Maréchal, Jean-Pierre Mahy, and Rémy Ricoux. 2022. "Photocatalytic Hydrogen Production and Carbon Dioxide Reduction Catalyzed by an Artificial Cobalt Hemoprotein" International Journal of Molecular Sciences 23, no. 23: 14640. https://doi.org/10.3390/ijms232314640