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Article

Polyoxypregnane Glycosides from Root of Marsdenia tenacissima and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells

1
Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China
2
College of Science, Yunnan Agricultural University, Kunming 650201, China
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(2), 886; https://doi.org/10.3390/molecules28020886
Submission received: 7 December 2022 / Revised: 9 January 2023 / Accepted: 12 January 2023 / Published: 16 January 2023
(This article belongs to the Special Issue Natural Products from Medicinal Plants)

Abstract

:
Six new polyoxypregnane glycosides, marstenacisside F1–F3 (13), G1–G2 (45) and H1 (6), as well as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B (7), were isolated from the roots of Marsdenia tenacissima. Their structures were established by an extensive interpretation of their 1D and 2D NMR and HRESIMS data. Compounds 17 were tenacigenin B derivatives with an oligosaccharide chain at C-3. This was the first time that compound 7 had been isolated from the title plant and its 1H and 13C NMR data were reported. Compounds 4 and 5 were the first examples of C21 steroid glycoside bearing unique β-glucopyranosyl-(1→4)-β-glucopyranose sugar moiety. All the isolated compounds were evaluated for anti-inflammatory activity by inhibiting nitric oxide (NO) production in the lipopolysaccharide-induced RAW 264.7 cells. The results showed that marstenacisside F1 and F2 exhibited significant NO inhibitory activity with an inhibition rate of 48.19 ± 4.14% and 70.33 ± 5.39%, respectively, at 40 μM, approximately equal to the positive control (L-NMMA, 68.03 ± 0.72%).

1. Introduction

Marsdenia tenacissima (Roxb.) Moon (Asclepiadaceae), a perennial climber, is distributed mainly in the southwest of China and other parts of tropical and subtropical Asia. The stems and roots of M. tenacissima are traditional Chinese medicine and Dai herbal medicine, respectively. The dried stems of M. tenacissima, known as “tongguanteng”, have been used in the treatment of asthma, cancer, and trachitis [1]. The roots of this plant, known as “Dai-Bai-Jie”, have been widely used as a Dai herbal medicine by Dai people living in Laos, Myanmar and the Yunnan province of China due to the root’s pharmacological functions of relieving pain, clearing heat, decreasing swelling and detoxification, etc. [2]. There have been many more chemical investigations on the stems than the roots of M. tenacissima. Previous phytochemical studies on the stems had revealed this plant as an extremely rich source of C21 steroid glycosides [3,4,5,6,7,8,9,10,11,12,13,14]. Although “Dai-Bai-Jie” has been widely used as a Dai herbal medicine, few phytochemical studies on the roots of this plant have been reported so far [15,16,17]. These studies showed that the main chemical composition of roots was also the same as the stems, i.e., C21 steroid glycosides. These compounds were only screened for anti-HIV activity and were necessary for anti-inflammatory activity, because the traditional usage for “Dai-Bai-Jie” was the treatment of inflammatory-associated diseases. Inflammation is a response of the organism to injury related to physical or chemical noxious stimuli or microbiological toxins, which is involved in multiple pathologies such as arthritis, asthma, multiple sclerosis, colitis, inflammatory bowel diseases, and atherosclerosis [18]. It can be speculated that the presence of key chemical constituents with effective anti-inflammatory activity had led to the extensive clinical application of “Dai-Bai-Jie” in traditional ethnomedicine, so we were interested in clarifying the relationship between the constituents and anti-inflammatory activity of this plant.
In order to search for more novel natural products, particularly those with potential anti-inflammatory activity, from the roots of M. tenacissima, a systematic phytochemical study was carried out on their 95% ethanol extract. As a result, six new polyoxypregnane glycosides, named marstenacisside F1–F3 (13), G1–G2 (45), and H1 (6), as well as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B (7), were isolated (Figure 1). Compound 7 was isolated from the title plant, and its 1H and 13C NMR data were reported, for the first time. In the present paper, we describe the isolation and structure elucidation of these compounds, and we also evaluate the anti-inflammatory activity of the isolated compounds in terms of the inhibitory effect on NO production in LPS-induced RAW 264.7 cells.

2. Results and Discussion

2.1. Structural Elucidation

Compound 1 displayed a sodium adduct ion at m/z 877.4344 [M + Na]+ in its HRESIMS spectrum (Figure S1), and its molecular formula was determined as C47H66O14 (calcd for C47H66NaO14, 877.4345). There were three methyl signals at δH 1.18 (3H, s, CH3-18), 1.09 (3H, s, CH3-19), and 2.26 (3H, s, CH3-21), and three methine protons bearing secondary alcoholic functions at δH 3.60 (m, H-3), 5.58 (t, J = 10.2 Hz, H-11) and 5.25 (d, J = 10.2 Hz, H-12) in the 1H NMR spectrum of 1. The combination of the 1H and 13C NMR data (Figures S2 and S3) indicated a C21 steroidal skeleton for 1. By comparison with C21 steroids isolated from the title plant, the 13C NMR data (Table 1) of 1 were similar to those of 11,12-diester of tenacigenin B [19]. The signals at δH 6.55 (q, J = 6.8 Hz), 1.46 (m), and 1.44 (s), and δC 167.4, 138.1, 128.5, 14.2, and 11.6, indicated the existence of a tigloyl (Tig) group. The long-range correlation from δH 5.58 (H-11) to δC 167.4 (Tig-C-1) in the HMBC spectrum (Figure 2) was observed, which indicated the Tig group was assigned at C-11. At the same time, there was a benzoyl (Bz) group in 1, due to the existence of a series of NMR signals at δH 7.89 (d, J = 7.4 Hz), 7.51 (t, J = 7.4 Hz), and 7.38 (t, J = 7.4 Hz), and δC 166.1, 133.1, 129.7 (2C), 129.4 and 128.3 (2C). The Bz group was demonstrated to be attached at C-12 by the HMBC correlation of H-12 (δH 5.25) to the carbonyl carbon (δC 166.1) of the benzoyl group. In the NOESY spectrum of 1 (Figure 3), crossing peaks between H-11 and CH3-18 (δH 1.18) and between H-12 and H-9 (δH 2.08) revealed that H-11 and H-12 were in β-orientation and α-orientation, respectively. Furthermore, the C-17 side chain was in an α-orientation as supported by the NOESY correlations between H-17 (δH 2.95) and CH3-18 (δH 1.18), and between H-12 (δH 5.25) and CH3-21 (δH 2.26) (Figure S7). Thus, the aglycone of 1 was determined to be 11α-O-tigloyl-12β-O-benzoyl-tenacigenin B.
In the NMR spectra of 1, there were two anomeric proton signals at δH 4.58 (dd, J = 9.8, 1.8 Hz) and 4.79 (d, J = 8.1 Hz) and two carbon signals at δc 96.9 and 99.1. The above evidence proved that the sugar moiety of 1 contained two units. The coupling constants (8.1 and 9.8 Hz) of the two anomeric protons indicated that both glycosidic linkages were β-oriented. At the same time, there were characteristic proton signals of two methyls at δH 1.36 (d, J = 5.5 Hz) and 1.25 (d, J = 6.0 Hz); two methoxyl groups at δH 3.66 (s) and 3.37 (s) in the 1H NMR spectrum of 1. The evidence of two methoxyl groups located at C-3 in each of the two sugar moiety was deduced from the long-range correlations between the methoxyl group at δH 3.37 (s) and the carbon signal at δC 78.8, and between another methoxyl group at δH 3.66 (s) and the carbon signal at δC 81.0. Subsequently, the two sugar units were identified as 6-deoxy-3-methoxy sugars, which generally occur in M. tenacissima [1]. Based on the HSQC, HMBC, and 1H-1H COSY spectra (Figure S4–S6), the NMR spectra of each sugar were fully assigned (Table 2 and Table 3). The two sugar units were then determined as oleandropyranosyl (Ole) and 6-deoxy-3-O-methyl-allopyranose (Allo), respectively [20]. Meanwhile, the oleandrose was the inner sugar and 6-deoxy-3-O-methyl allose was the outer, which was supported by the HMBC correlations of δH 4.79 (Allo-H-1) with δC 79.1 (Ole-C-4), and δH 4.58 (Ole-H-1) with δC 76.4 (aglycone-C-3). Consequently, the sugar moiety of 1 was determined as pachybiose. Compared to the previously reported data of tenacigenin B [21], changes in the chemical shift in aglycone of 1, i.e., C-2 (−3.2 ppm), C-3 (+5.6 ppm), and C-4 (−3.8 ppm) were observed, which suggested that the sugar moiety was linked to the C-3 hydroxyl of the aglycone. Thus, compound 1 was elucidated as 3-O-6-deoxy-3-O-methyl-β-D-allopyranosyl (1→4)-β-D-oleandropyranosyl-11α-O-tigloyl-12β-O-benzoyl-tenacigenin B and named marstenacisside F1.
The 1H and 13C NMR spectroscopic data ascribed to the sugar moieties of 23 are consistent with those of 1 (Table 2 and Table 3), so they should contain the same sugar moiety as 1.
Compound 2 possessed a molecular formula of C49H64O14, determined by HRESIMS ion at m/z 899.4190 [M + Na]+ (calcd for C49H64NaO14, 899.4188) (Figure S8). The NMR data of 2 showed a pattern analogous to 1, except for an ester group (Figures S9 and S10). In the 13C NMR spectrum of 2, there were signals of two benzoyl groups on the aglycone of 2. Meanwhile, the tigloyl unit signals of 1 were absent in 2. An extra Bz group was positioned at C-11, which was deduced from the HMBC correlation from δH 5.77 (H-11) to δC 166.2 (Bz1-C-1) (Figure S13). Due to the HMBC correlation between δH 4.58 (Ole-H-1) and δC 76.4 (aglycone-C-3), the glycosidation site was located at C-3 of the aglycone. Consequently, 2 was defined as 3-O-6-deoxy-3-O-methyl-β-D-allopyanosyl(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B, and named marstenacisside F2 (Figures S11–S14).
Compound 3 showed a quasi-molecular ion peak at m/z 825.4040 [M + Na]+, in accordance with the molecular formula C43H62O14 (calcd for C43H62NaO14, 825.4032) (Figure S15). In the 1H NMR spectrum of 3 (Figure S16), due to the absence of an ester group at C-12, there was a higher-field shift signal at δH 3.27 (1H, d, J = 9.7 Hz), compared to the δH 5.35 (1H, d, J = 10.2 Hz) in the 1H NMR spectrum of 2. Consequently, the aglycone of 3 was a monoester of tenacigenin B. The signals at δH 7.09 and 6.75 (d, J = 8.6 Hz, each 2H) and the 13C NMR (Figure S17) singlet at δC 155.2 indicated that 3 contained a 4-hydroxyphenyl group. In the HMBC spectrum (Figure S20), the aromatic resonances at δC 125.3 and 130.7 were correlated with the signal at δH 3.54 (m, 2H), and 7.09 correlated with the δC 41.1. Moreover, the HMBC spectrum showed a correlation between δH 3.54 and δC 172.0. Therefore, a methylene group (δH 3.54, δC 41.1) was located between the 4-hydroxyphenyl moiety and the carbonyl group (δC 172.0). Hence, 3 contained a (4-hydroxyphenyl) acetyl (HPA) group. The HPA group was assigned at C-11 by HMBC correlation of the proton at δH 5.05 (H-11) with the carbonyl carbon at δC 172.0 of the HPA group. The glycosidation site was deduced from the HMBC cross peaks between δH 4.58 (Ole-H-1) and δC 76.6 (aglycone-C-3). Therefore, 3 was defined as 3-O-6-deoxy-3-O-methyl-β-D-allopyanosyl (1→4)-β-D-oleandropyranosyl-11α-O-(4-hydroxyphenyl) acetyl-tenacigenin B and named marstenacisside F3 (Figures S18–S21).
Compound 4 had a molecular formula of C40H60O17, as determined by HRESIMS [M + Na]+ ion at m/z 835.3714 (calcd for C40H60NaO17, 835.3723) (Figure S22). The proton signals of δH 1.90 (3H, s), 6.86 (1H, qq, J = 7.1, 1.4 Hz), 1.80 (3H, s), and 1.61 (3H, d, J = 7.1 Hz) and the carbon signals of δC 170.7, 20.5, 167.2, 129.2, 138.1, 12.2, and 14.3 indicated the presence of an acetyl (Ac) and a tigloyl group in the agcylone of 4. The 1H NMR data of the aglycone moiety of 4 were very close to those of 1, except for the presence of the signal for an acetyl group at δH 1.90 (3H, s) and the absence of the protons signals for the benzoyl group. HMBC cross-peaks between δH 5.76 (H-11) and δC 167.2 (Tig-C-1), and between δH 5.40 (H-12) and δc 170.7 (Ac-C-1) (Figure S27), indicated that a tigloyl and acetyl group were located at C-11 and C-12, respectively. Accordingly, the aglycone of 4 was identified as 11α-O-tigloyl-12β-O-acetyl-tenacigenin B.
In the NMR spectra of 4 (Figures S23 and S24), there were two anomeric proton signals at δH 5.01 (d, J = 7.6 Hz) and 5.22 (d, J = 7.8 Hz) and two carbon signals at δc 101.2 and 106.7. The above facts proved that the sugar moiety of 4 contained two units. Furthermore, the 13C NMR spectra displayed two terminal oxygenated methylene groups at δc 62.6. On the basis of the above evidence and compared with previously reported data [22], the two sugar units were identified as glucopyranoses. According to the coupling constants of anomeric protons (7.6 and 7.8 Hz), the linkages of two sugar units were in β-configuration. The linkage of two sugar moiety could be β-glucopyranosyl-(1→4)-β-glucopyranoside deduced from the long-range correlations between δH 5.22 (Glc2-H-1) and δc 84.7 (Glc1-C-4). Relative to the previously reported values of 11α-O-tigloyl-12β-O-acetyl-tenacigenin B [23], changes in the chemical shift in aglycone of 4, i.e., C-2 (−1.8 ppm), C-3 (+8.4 ppm), and C-4 (−2.9 ppm) were detected, which suggested that the sugar moiety was attached at the C-3 of the aglycone. Thus, compound 4 was finally elucidated as 3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-11α-O-tigloyl-12β-O-acetyl-tenacigenin B and named marstenacisside G1 (Figures S25–S28).
The 1H and 13CNMR data of the sugar moiety of 5 were well in agreement with those of 4. Accordingly, 5 had the same sugar moiety as 4.
Compound 5 exhibited a molecular formula of C40H62O17 based on the HRESIMS ion [M + Na]+ at m/z 837.3883 (calcd for C40H62NaO17, 837.3879) (Figure S29). The NMR data of 5 (Figures S30 and S31) showed a similar pattern to 4, except for an ester substitution. In the 1H NMR spectrum of 5, there were proton signals of an acetyl group at δH 2.02 (3H, s) and extra signals of 2-methylbutyryl ester units at δH 0.84 (3H, t, J = 7.5 Hz), 1.04 (3H, d, J = 7.0 Hz), 1.34 (1H, m), 1.68 (1H, m), and 2.26 (1H, m). Meanwhile, the signals of the tigloyl unit of 4 were absent in 5. The HMBC cross peak between the carbonyl carbon at δC 175.5 of the 2-methylbutyryl group and the proton signal at δH 5.68 (H-11) disclosed a 2-methylbutyryl group located at C-11 (Figure S34). Likewise, the HMBC correlation of the carbonyl carbon at δC 170.8 of the acetyl unit with the proton signal at δH 5.36 (H-12) revealed the existence of an acetyl group at C-12. The glycosidation site was deduced from the long-range coupling of the δH 5.08 (Glc1-H-1) with δC 77.9 (agclone-C-3). Accordingly, compound 5 was established as 3-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-11α-O-2-methylbutyryl-12β-O-acetyl-tenacigenin B and was named marstenacisside G2 (Figures S32–S35).
Compound 6 gave a molecular formula of C51H74O20 based on the HRESIMS (m/z 1029.4659 [M + Na]+, calcd for C51H74NaO20, 1029.4666) (Figure S36). 13C NMR data analysis indicated that the aglycone moiety of 6 differs from the aglycone moiety of 3 by the presence of an extra acetyl group (δC 170.8 and 20.3). The positions of the diester groups were deduced by the HMBC correlations between δH 5.68 (H-11) and δC 171.5 (HPA-C-1), and between δH 5.36 (H-12) and δC 170.8 (Ac-C-1) (Figure S41). Therefore, the aglycone structure of 6 was identified as 11α-O-(4-hydroxyphenyl) acetyl-12β-O-acetyl-tenacigenin B.
The 1H and 13C NMR spectra of 6 (Figures S37 and S38) exhibited three anomeric proton signals at δH 5.27 (1H, d, J = 8.1 Hz), 4.97 (1H, d, J = 7.8 Hz), and 4.78 (1H, d, J = 8.9 Hz) and three anomeric carbon signals at δC 102.0, 106.6 and 97.5, suggesting the existence of three sugar units in the molecule. Due to the large coupling constants of anomeric protons, the linkages of three sugar units were in β-configuration. At the same time, there were characteristic proton signals of two methyls at δH 1.67 (d, J = 5.8 Hz) and 1.63 (d, J = 6.2 Hz); two methoxyl groups at δH 3.81 (s) and 3.50 (s); and two ABM spin protons at δH 4.36 (dd, J = 11.6, 5.4 Hz), 4.54 (dd, J = 11.6, 2.3 Hz) in the 1H NMR spectrum of 6. On the basis of the above evidence and compared with previously reported data [24,25], the sugar units were identified as oleandrose, 6-deoxy-3-O-methyl-allose, and glucoses. The connectivity of the sugars was established by the HMBC correlations between δH 4.97 (Glc-H-1) and δC 83.4 (Allo-C-4); between δH 5.27 (Allo-H-1) and δC 83.4 (Ole-C-4). As a result, the sugar moiety was determined as β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranoside, which was identical to the neocondurangotriose in the compounds that were also isolated from M. tenacissima [17]. Furthermore, the glycosidation site was deduced from the long-range correlations between δH 4.78 (Ole-H-1) and δC 76.0 (aglycone-C-3). Hence, the structure of 6 was established as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α-O-(4-hydroxyphenyl) acetyl-12β-O-acetyl-tenacigenin B and named marstenacisside H1 (Figures S39–S42).
Compound 7 had a molecular formula of C55H74O19 determined by HRESIMS ion [M + Na]+ at m/z 1061.4718 (calcd for C55H74NaO19, 1061.4722) (Figure S43). Compound 7 was predicted to be novel pregnane glycoside in a crude extract of Marsdenia tenacissima by means of LC-ESI-MSn [26], and the compound was not isolated from the crude extract. This is the first time that compound 7 has been isolated from the title plant and its 1H and 13C NMR data were reported. The structure of 7 was eluciated as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B, on the basis of 1D, 2D NMR and HRESI data (Figures S44–S49).

2.2. NO Inhibitory Evaluations

Compounds 17 were screened for anti-inflammatory activity by inhibiting NO production in LPS-induced RAW 264.7 cells. Compounds 1 and 2 showed significant NO inhibitory activity with an inhibition rate of 48.19 ± 4.14% and 70.33 ± 5.39%, respectively, at 40 μM, approximately equal to the positive control (L-NMMA, 68.03 ± 0.72%) (Figure 4 and Table 4). The effects of compounds 17 on cell viability are shown in Figure S50. All compounds showed dose-dependent NO inhibitory activity. Only 1 and 2 showed significant NO inhibitory activity, and other compounds did not show the activity. The above facts may be related to the structure of compounds 1 and 2. There were tigloyl and/or benzoyl groups at C-11 and C-12 of 1 and 2, and the sugar moiety was pachybiose. Although 7 also had two benzoyl groups at C-11 and C-12, the sugar moiety was neocondurangotriose.

3. Materials and Methods

3.1. General Experimental Procedures

The UV spectra were collected on a Shimadzu UV-2401 PC spectrophotometer (Shimadzu Corp: Kyoto, Japan). Optical rotations were measured on an Autopol VI polarimeter; The IR spectra were determined on a Nicolet iS10 spectrometer with KBr pellets. HRESIMS was recorded on an Agilent 1290 UPLC/6540 Q-TOF mass spectrometer (Agilent, Palo Alto, CA, USA). All NMR spectra were acquired on a Bruker Avance III 500 spectrometer. Semi-preparative HPLC was performed on a Waters HPLC system consisting of a 1525 binary pump and a 2487 detector, equipped with a YMC-pack ODS-A column (250 × 10 mm, YMC Co., Ltd., Kyoto, Japan). Silica gel (200–300 mesh, Qingdao Marine Chemical Co., Ltd., Qingdao, China), Lichroprep RP-18 gel (40–63 μm, Merck, Darmstadt, Germany), Sephadex LH-20 gel (GE Healthcare, Sweden), and MCI gel (75–150 μm, Mitsubishi Chemical Co., Tokyo, Japan) were used to perform column chromatography.

3.2. Plant Material

The roots of Marsdenia tenacissima were collected from Simao, Yunnan Province, China in January 2020. A voucher specimen (No. 20200101) was deposited in the authors’ research group.

3.3. Extraction and Isolation

The dried powder roots of Marsdenia tenacissima (2.5 kg) were percolated with 95% ethanol at room temperature three times (3 days each time) and then concentrated under reduced pressure to give concentrated extract. The concentrated extract was efficiently partitioned with ethyl acetate (EtOAc). The EtOAc fraction (68.6 g) was separated by an MCI gel CHP 20P column, eluted with MeOH–H2O (v/v, 30:70, 50:50, 80:20, 95:5) to provide four portions (Fr. A–D). Fr. B (28.1 g) was subjected to silica gel CC eluted with CH2Cl2–MeOH (25:1–3:1) to obtain five fractions (Fr. B.1–5). Fr. B.2 (2.8 g) was chromatographed over a Sephadex LH-20 column, eluting with MeOH to give four fractions (Fr. B.2.1–4). Fr. B.2.2 (256 mg) was further purified by semi-preparative HPLC using MeOH/H2O (70:30, 3 mL/min) to give compounds 3 (12 mg, tR = 12.3 min) and 6 (9 mg, tR = 19.6 min). Fr. B.4 (2.1 g) was separated by ODS MPLC (MeOH–H2O, 60:40 to 100:0) to yield five fractions (Fr. B.4.1–5). Fr. B.4.3 (202 mg) was further separated by semi-preparative with MeOH/H2O (80:20, 3 mL/min) to yield compounds 4 (11 mg, tR = 8.3 min) and 5 (8 mg. tR = 9.6 min). Fr. C (35.7 g) was subjected to silica gel CC eluted with CH2Cl2–MeOH (50:1–4:1) to obtain five fractions (Fr. C.1–5). Fr. C.3 (3.2 g) was chromatographed over a Sephadex LH-20 column, eluting with CH2Cl2–MeOH (1:1) to give four fractions (Fr. C.3.1–4). Fr. C.3.3 (306 mg) was further purified by semi-preparative HPLC using MeOH/H2O (75:25, 3 mL/min) to afford compounds 7 (11 mg, tR = 12.3 min), 1 (9 mg, tR = 16.6 min), and 2 (10 mg, tR = 20.7 min).

3.4. Compound Characterization Data

Marstenacisside F1 (1): white amorphous powder; [α ] D 23 + 19.6 (c 0.15, MeOH); UV (MeOH) λmax (log ε): 196 (4.46), 226 (4.26), 273 (3.01) nm; IR (KBr): υmax 3436, 2929, 1719, 1451, 1367, 1281, 1164, 1071, 988, 711 cm−1; 1H NMR (CDCl3) data of aglycone moiety of 1: δ 1.09 (3H, s, 19-CH3), 1.18 (3H, s, 18-CH3), 1.44 (3H, brs, Tig-H-5), 1.46 (3H, m, Tig-H-4), 2.08 (1H, m, H-9), 2.26 (3H, s, 21-CH3), 2.99 (1H, d, J = 6.4 Hz, H-17β), 3.60 (1H, m, H-3), 5.25 (1H, d, J = 10.2 Hz, H-12α), 5.58 (1H, t, J = 10.2 Hz, H-11β), 6.55 (1H, q, J = 6.8 Hz, Tig-H-3), 7.38 (2H, t, J = 7.4 Hz, Bz-H-4, 6), 7.51 (1H, t, J = 7.4 Hz, Bz-H-5), 7.89 (2H, d, J = 7.4 Hz, Bz-H-3, 7); HRESIMS: m/z 877.4344 [M + Na]+ (calcd for C47H66NaO14, 877.4345); for 13C NMR data of the aglycone moiety of 1 see Table 1. For 1H and 13C NMR data of the sugar moiety of 1 see Table 2 and Table 3.
Marstenacisside F2 (2): white amorphous powder; [α ] D 23 + 26.9 (c 0.18, MeOH); UV (MeOH) λmax (log ε): 196 (4.49), 230 (4.33), 274 (3.34) nm; IR (KBr): υmax 3436, 2932, 1721, 1451, 1367, 1283, 1162, 1070, 988, 708 cm−1; 1H NMR (CDCl3) data of aglycone moiety of 2: δ 1.14 (3H, s, 19-CH3), 1.21 (3H, s, 18-CH3), 2.21 (1H, m, H-9), 2.27 (3H, s, 21-CH3), 3.01 (1H, d, J = 7.3 Hz, H-17β), 3.60 (1H, m, H-3), 5.35 (1H, d, J = 10.2 Hz, H-12α), 5.77 (1H, t, J = 10.2 Hz, H-11β), 7.18 (2H, t, J = 7.4 Hz, Bz2-H-4, 6), 7.22 (2H, t, J = 7.4 Hz, Bz1-H-4, 6), 7.33 (1H, t, J = 7.4 Hz, Bz2-H-5), 7.37 (1H, t, J = 7.4 Hz, Bz1-H-5), 7.75 (4H, d, J = 7.4 Hz, Bz1-H-3, 7, Bz2-3, 7); HRESIMS: m/z 899.4190 [M + Na]+ (calcd for C49H64NaO14, 899.4188); for 13C NMR data of the aglycone moiety of 2 see Table 1. For 1H and 13C NMR data of the sugar moiety of 2 see Table 2 and Table 3.
Marstenacisside F3 (3): white amorphous powder; [α ] D 19 − 4.9 (c 0.10, MeOH); UV (MeOH) λmax (log ε): 196 (4.16), 224 (3.69), 278 (3.08) nm; IR (KBr): υmax 3445, 2933, 1703, 1619, 1367, 1164, 1068, 989, 610 cm−1; 1H NMR (CDCl3) data of aglycone moiety of 3: δ 1.03 (3H, s, 19-CH3), 1.14 (3H, s, 18-CH3), 1.85 (1H, m, H-9), 2.26 (3H, s, 21-CH3), 3.01 (1H, t, J = 6.2 Hz, H-17β), 3.27 (1H, d, J = 9.7 Hz, H-12α), 3.54 (2H, m, HPA-2), 3.66 (1H, m, H-3), 5.05 (1H, d, J = 9.7 Hz, H-11β), 6.75 (2H, d, J = 8.6 Hz, HPA-H-5, 7), 7.09 (2H, d, J = 8.6 Hz, HPA-H-4, 8); HRESIMS: m/z 825.4040 [M + Na]+ (calcd for C43H62NaO14, 825.4032); for 13C NMR data of the aglycone moiety of 3 see Table 1. For 1H and 13C NMR data of the sugar moiety of 3 see Table 2 and Table 3.
Marstenacisside G1 (4): white amorphous powder; [α ] D 23 + 2.1 (c 0.10, MeOH); UV (MeOH) λmax (log ε): 196 (3.93), 216 (3.94) nm; IR (KBr): υmax 3428, 2934, 1706, 1619, 1368, 1269, 1077, 1031 cm−1; 1H NMR (pyridine-d5) data of aglycone moiety of 4: δ 1.20 (3H, s, 19-CH3), 1.28 (3H, s, 18-CH3), 1.61 (3H, d, J = 7.1 Hz, Tig-H-5), 1.80 (3H, brs, Tig-H-4), 2.05 (1H, m, H-9), 1.90 (3H, s, Ac-H-2), 2.23 (3H, s, 21-CH3), 2.90 (1H, d, J = 6.7 Hz, H-17β), 3.88 (1H, m, H-3), 5.40 (1H, d, J = 10.2 Hz, H-12α), 5.76 (1H, t, J = 10.2 Hz, H-11β), 6.86 (1H, qq, J = 7.1, 1.4 Hz, Tig-H-3); HRESIMS: m/z 835.3714 [M + Na]+ (calcd for C40H60NaO17, 835.3723); for 13C NMR data of the aglycone moiety of 4 see Table 1. For 1H and 13C NMR data of the sugar moiety of 4 see Table 2 and Table 3.
Marstenacisside G2 (5): [α ] D 22 + 13.3 (c 0.10, MeOH); UV (MeOH) λmax (log ε): 196 (3.83), 228 (3.48), 274 (2.72) nm; IR (KBr): υmax 3426, 2936, 1736, 1632, 1367, 1247, 1076, 1030 cm−1; 1H NMR (pyridine-d5) data of aglycone moiety of 5: δ 0.84 (3H, t, J = 7.5 Hz, mBu-H-4), 1.04 (3H, d, J = 7.0 Hz, mBu-H-5), 1.13 (3H, s, 19-CH3), 1.25 (3H, s, 18-CH3), 1.34 and 1.68 (2H, m, mBu-H-3), 2.02 (3H, s, Ac-H-2), 2.00 (1H, m, H-9), 2.26 (1H, m, mBu-H-2), 2.24 (3H, s, 21-CH3), 2.87 (1H, d, J = 6.9 Hz, H-17β), 3.84 (1H, m, H-3), 5.36 (1H, d, J = 10.2 Hz, H-12α), 5.68 (1H, t, J = 10.2 Hz, H-11β); HRESIMS: m/z 837.3883 [M + Na]+ (calcd for C40H62NaO17, 837.3879); for 13C NMR data of the aglycone moiety of 5 see Table 1. For 1H and 13C NMR data of the sugar moiety of 5 see Table 2 and Table 3.
Marstenacisside H1 (6): white amorphous powder; [α ] D 22 − 1.6 (c 0.10, MeOH); UV (MeOH) λmax (log ε): 197 (4.38), 225 (3.98), 277 (3.57) nm; IR (KBr): υmax 3445, 2935, 1738, 1516, 1367, 1253, 1071, 991 cm−1; 1H NMR (pyridine-d5) data of aglycone moiety of 6: δ 1.13 (3H, s, 18-CH3), 1.17 (3H, s, 19-CH3), 1.77 (3H, s, Ac-H-2), 2.07 (1H, d, J = 10.1 Hz, H-9), 2.24 (3H, s, 21-CH3), 2.86 (1H, d, J = 6.7 Hz, H-17β), 3.82 (1H, m, H-3), 5.36 (1H, d, J = 10.2 Hz, H-12α), 5.68 (1H, t, J = 10.2 Hz, H-11β), 7.11 (2H, d, J = 8.4 Hz, HPA-H-5, 7), 7.29 (2H, d, J = 8.6 Hz, HPA-H-4, 8); HRESIMS: m/z 1029.4659 [M + Na]+ (calcd for C51H74NaO20, 1029.4666); for 13C NMR data of the aglycone moiety of 6 see Table 1. For 1H and 13C NMR data of the sugar moiety of 6 see Table 2 and Table 3.
3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B (7): white amorphous powder; [α ] D 21 + 20.8 (c 0.23, MeOH); UV (MeOH) λmax (log ε): 201 (4.18), 230 (4.24), 274 (3.16) nm; IR (KBr): υmax 3427, 2934, 1721, 1452, 1367, 1281, 1071, 710 cm−1; 1H NMR (pyridine-d5) data of aglycone moiety of 7: δ 1.29 (3H, s, 18-CH3), 1.31 (3H, s, 19-CH3), 2.09 (1H, m, H-9), 2.32 (3H, s, 21-CH3), 2.97 (1H, d, J = 6.3 Hz, H-17β), 3.78 (1H, m, H-3), 5.78 (1H, d, J = 10.2 Hz, H-12α), 6.13 (1H, t, J = 10.2 Hz, H-11β), 7.16 (2H, t, J = 7.4 Hz, Bz2-H-4, 6), 7.22 (2H, t, J = 7.4 Hz, Bz1-H-4, 6), 7.24 (1H, t, J = 7.4 Hz, Bz2-H-5), 7.43 (1H, t, J = 7.4 Hz, Bz1-H-5), 7.94 (2H, d, J = 7.4 Hz, Bz2-H-3, 7), 7.99 (2H, d, J = 7.4 Hz, Bz1-H-3, 7); HRESIMS: m/z 1061.4718 [M + Na]+ (calcd for C55H74NaO19, 1061.4722); for 13C NMR data of the aglycone moiety of 7 see Table 1. For 1H and 13C NMR data of the sugar moiety of 7 see Table 2 and Table 3.

3.5. Cell Culture and Nitric Oxide Inhibitory Assay

The macrophage RAW 264.7 cells (passage number was 10–13) were obtained from Cell Bank of Chinese Academy of Sciences. The RAW 264.7 cells were plated in 96-well plates (1.5 × 105 cells/well) and treated with different isolate concentrations (dissolved in DMSO) of 10, 20, and 40 μM, respectively, followed by stimulation with 1 μg/mL LPS (Sigma, St. Louis, MO, USA) for 18 h [27]. Griess reagents (Sigma, St. Louis, MO, USA) were used to measure NO production. The optical density (OD) was determined at a 570 nm wavelength, with L-NMMA as a positive control [28]. Three independent experiments were carried out in triplicate. The cell viability was evaluated by the MTT assay [29].

4. Conclusions

Six new polyoxypregnane glycosides, marstenacisside F1–F3 (13), G1–G2 (45), and H1 (6), as well as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B (7), were isolated from the ethanolic extract of the roots of Marsdenia tenacissima by modern chromatographic techniques and characterized by comprehensive spectroscopic data. Their structures were tenacigenin B derivatives with an oligosaccharide chain at C-3. Compounds 4 and 5 were the first examples of C21 steroid glycoside bearing unique β-glucopyranosyl-(1→4)-β-glucopyranose sugar moiety. Compound 7 was isolated from the title plant for the first time, and its 1H and 13C NMR data were reported. The patterns of compounds 17 were consistent with those of compounds previously isolated from this plant. All isolates were evaluated for anti-inflammatory activity by inhibiting the production of NO stimulated by LPS in RAW 264.7 cells, with L-NMMA as a positive control. Among those compounds, compounds 1 and 2 exhibited significant NO inhibition at 40 μM.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/molecules28020886/s1; Figures S1–S49: HRESIMS, 1D and 2D NMR of compounds 17. Figure S50: Effects of compounds 17 on cell viability.

Author Contributions

Z.N. designed the experiment, performed the isolation and identification of all the compounds, and also wrote this paper; P.G. contributed to the nitric oxide inhibition assay, data analysis, and wrote this section; Q.F. provided comments and suggestions on structure elucidation and reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work was financially supported by the Chinese Academy of Sciences (CAS) 135 Program (XTBG-F02) and Yunnan Fundamental Research Projects (grant NO. 202101AT070057).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All the data in this research were presented in the manuscript and Supplementary Material.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of compounds 17 are available from the authors.

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Figure 1. Structures of pregnane glycosides (17) isolated from M. tenacissima.
Figure 1. Structures of pregnane glycosides (17) isolated from M. tenacissima.
Molecules 28 00886 g001
Figure 2. Key HMBC and 1H-1H COSY correlations of 1.
Figure 2. Key HMBC and 1H-1H COSY correlations of 1.
Molecules 28 00886 g002
Figure 3. Key ROESY correlations of the aglycone of 1.
Figure 3. Key ROESY correlations of the aglycone of 1.
Molecules 28 00886 g003
Figure 4. Inhibitory effects of compounds 17 on NO production in LPS-induced RAW 264.7 cells. Experiments were performed in triplicate, and the data are presented as the mean ± SD. ** p < 0.01, *** p < 0.001 vs. LPS group. NS, not significant by T-test.
Figure 4. Inhibitory effects of compounds 17 on NO production in LPS-induced RAW 264.7 cells. Experiments were performed in triplicate, and the data are presented as the mean ± SD. ** p < 0.01, *** p < 0.001 vs. LPS group. NS, not significant by T-test.
Molecules 28 00886 g004
Table 1. 13C NMR data of the aglycones of compounds 17 in pyridine-d5 (125 MHz, δ in ppm).
Table 1. 13C NMR data of the aglycones of compounds 17 in pyridine-d5 (125 MHz, δ in ppm).
Position1 a2 a3 a4567
137.437.638.537.838.137.937.8
229.129.128.929.829.629.829.7
376.476.476.677.877.976.075.9
434.734.834.535.235.135.235.2
544.044.044.544.043.943.943.9
626.726.727.027.227.127.227.3
731.831.932.325.225.225.125.3
866.966.966.066.866.866.766.9
951.351.354.351.951.851.751.8
1039.139.239.339.439.439.439.5
1168.869.568.669.168.869.069.9
1275.275.574.275.275.274.975.5
1346.146.247.344.046.146.146.5
1471.571.671.671.771.671.771.9
1526.826.827.732.232.132.132.1
1625.125.125.427.127.127.127.2
1759.859.960.359.760.059.959.9
1816.616.717.516.917.016.916.9
1912.712.812.913.213.213.113.2
20211.1211.1212.6210.3210.0210.1210.4
2130.330.232.730.329.930.030.2
11-OTigBzHPATigmBuHPABz
1167.4166.2172.0167.2175.5171.5166.5
2128.5130.041.1129.241.541.4130.6
3138.1129.5125.3138.126.6124.6129.9
411.6128.1130.712.211.9131.3128.7
514.2132.9115.614.315.7116.4133.5
6 128.1155.2 158.2128.7
7 129.5115.6 116.4129.9
8 130.7 131.3
12-OBzBz AcAcAcBz
1166.1166.1 170.7170.8170.8166.3
2129.4129.0 20.520.920.3130.6
3129.7129.5 129.8
4128.3128.1 128.7
5133.1132.8 133.3
6128.3128.1 128.7
7129.7129.5 129.8
a Measured in CDCl3.
Table 2. 13C NMR data of the sugar moieties of compounds 17 in pyridine-d5 (125 MHz, δ in ppm).
Table 2. 13C NMR data of the sugar moieties of compounds 17 in pyridine-d5 (125 MHz, δ in ppm).
Position1 a2 a3 a4567
OleOleOleGlc-1Glc-1OleOle
196.997.097.0101.2101.197.597.4
236.136.136.171.471.537.937.8
378.878.878.878.078.079.779.6
479.179.179.184.784.783.483.3
571.471.471.478.077.971.971.9
618.618.618.662.662.619.119.0
3-OMe55.655.655.7 57.357.3
AlloAlloAlloGlc-2Glc-2AlloAllo
199.199.199.2106.7106.7102.0101.9
271.871.871.877.177.172.772.7
381.081.081.078.378.383.283.2
472.872.872.871.471.583.483.3
571.371.371.378.978.969.669.5
617.917.917.962.662.618.418.3
3-OMe62.061.962.0 61.761.7
GlcGlc
1 106.6106.6
2 75.575.5
3 78.478.4
4 72.072.0
5 78.578.4
6 63.063.0
a Measured in CDCl3.
Table 3. 1H NMR data of the sugar moieties of compounds 17 in pyridine-d5 (500 MHz, δ in ppm, J in Hz).
Table 3. 1H NMR data of the sugar moieties of compounds 17 in pyridine-d5 (500 MHz, δ in ppm, J in Hz).
Position1 a2 a3 a4567
OleOleOleGlc-1Glc-1OleOle
14.58 dd 4.58 dd4.58 dd5.01 d5.08 d4.78 d4.73 d
(9.8, 1.8)(9.8, 1.9)(9.7, 1.7)(7.6)(7.7)(8.9)(9.4)
21.47 m1.45 m1.49 m4.31 m4.31 m1.26 m1.33 m
2.30 m2.30 m2.30 m 2.41 m2.36 m
33.39 m3.38 m3.38 m4.25 m4.23 m3.61 m3.57 m
43.33 m3.31 m3.34 m4.09 m4.13 m3.59 m3.52 m
53.33 m3.31 m3.34 m3.92 m3.92 m3.64 m3.52 m
61.36 d 1.32 d 1.37 d4.33 m4.34 m1.67 d 1.57 d
(5.5)(5.5)(5.5)4.50 m4.52 m(5.8)(5.0)
3-OMe3.37 s3.35 s3.37 s 3.50 s3.47 s
AlloAlloAlloGlc-2Glc-2AlloAllo
14.79 d 4.77 d 4.79 d 5.22 d5.25 d 5.27 d 5.24 d
(8.1)(8.3)(8.3)(7.8)(7.8)(8.1)(8.1)
23.47 m3.46 m3.47 m4.11 m4.12 m3.84 m3.79 m
33.79 t 3.78 t3.78 t 4.35 m4.37 m4.47 t 4.45 m
(3.0)(3.0)(3.0) (2.5)
43.17 m3.17 m3.17 m4.21 m4.21 m3.73 dd3.72 dd
(9.6, 2.5)(9.4, 2.0)
53.55 m3.54 m3.55 m3.93 m3.94 m4.27 m4.23 m
61.25 d 1.24 d 1.25 d 4.33 m4.34 m1.63 d 1.61 d
(6.0)(6.4)(6.1)4.45 m4.43 m(6.2)(6.2)
3-OMe3.66 s3.65 s3.65 s 3.81 s3.80 s
GlcGlc
1 4.97 d4.95 d
(7.8)(7.7)
2 4.02 m4.00 m
3 4.26 m4.22 m
4 4.22 m4.19 m
5 4.01 m3.97 m
6 4.36 dd4.36 dd
(11.6, 5,4)(11.6, 5,1)
4.54 dd4.53 d
(11.6, 2.3)(11.6)
a Measured in CDCl3.
Table 4. Inhibitory effects of compounds 17 on NO production in LPS-induced RAW 264.7 cells.
Table 4. Inhibitory effects of compounds 17 on NO production in LPS-induced RAW 264.7 cells.
CompoundConcentration (μM)NO Inhibition Rate (%)
14048.19 ± 4.14
24070.33 ± 5.39
340−4.09 ± 7.28
440−0.86 ± 1.59
5400.80 ± 1.91
640−5.57 ± 1.15
7407.13 ± 5.00
L-NMMA a4068.03 ± 0.72
a Positive control.
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Na, Z.; Gongpan, P.; Fan, Q. Polyoxypregnane Glycosides from Root of Marsdenia tenacissima and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells. Molecules 2023, 28, 886. https://doi.org/10.3390/molecules28020886

AMA Style

Na Z, Gongpan P, Fan Q. Polyoxypregnane Glycosides from Root of Marsdenia tenacissima and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells. Molecules. 2023; 28(2):886. https://doi.org/10.3390/molecules28020886

Chicago/Turabian Style

Na, Zhi, Pianchou Gongpan, and Qingfei Fan. 2023. "Polyoxypregnane Glycosides from Root of Marsdenia tenacissima and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells" Molecules 28, no. 2: 886. https://doi.org/10.3390/molecules28020886

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