Inhibition of Bacterial Adhesion and Biofilm Formation by Seed-Derived Ethanol Extracts from Persea americana Mill
Abstract
:1. Introduction
2. Results
2.1. Plant Samples Preparation
2.2. Antibacterial Activity and Cytotoxicity of P. americana Seed Ethanol Extracts
2.3. Ethanol Extracts from P. americana Seeds Inhibit Bacterial Adhesion to A549 Epithelial Lung Cells
2.4. Ethanol Extracts from P. americana Seeds Inhibits P. aeruginosa Biofilms
2.5. Ethanol Extracts from P. americana Seeds Affect P. aeruginosa Membrane Integrity
2.6. Effect of Ethanol Extracts from P. americana Seeds on Virulence and Adhesion-Related Genes of P. aeruginosa
3. Discussion
4. Materials and Methods
4.1. Material and Reagents
4.2. Plant Samples Preparation
4.3. Cell Lines, Bacterial Strains, and Culture Conditions
4.4. Cytotoxicity Assessment
4.5. Antimicrobial Assessment
4.5.1. Antimicrobial Screening
Microdilution Method
Bacterial Adherence Assay
4.5.2. Biofilm Formation Assay
4.5.3. Measurement of Release of Nucleic Acids
4.6. Real Time PCR and Gel Electrophoresis Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Bacteria | IC50 | |||
---|---|---|---|---|
SE (µg/mL) | MaE (µg/mL) | Doxycycline (µM) | Ciprofloxacin (µM) | |
E. coli ATCC 8739 | ˃1024 | ˃1024 | 0.50 ± 0.28 | - |
P. aeruginosa ATCC 9027 | 87.0 ± 4.4 * | 187.4 ± 9.4 * | - | 1.05 ± 0.50 |
S. aureus ATCC 6538 | 144.2 ± 5.7 * | 159.2 ± 7.9 | 0.04 ± 0.95 | - |
S. pneumoniae NTCC 7466 | 227.6 ± 11.4 | 532.2 ± 26.6 | 3.56 ± 0.84 | - |
Cell Lines | CC50 | ||
---|---|---|---|
SE (µg/mL) | MaE (µg/mL) | Tamoxifen (µM) | |
MRC-5 | 636.9 ± 31.8 | 786.3 ± 39.3 | 8.7 ± 0.4 |
A549 | 770.0 ± 38.5 | 774.3 ± 38.7 | 6.1 ± 0.3 |
Gene | Forward Primer | Reverse Primer |
---|---|---|
mexT | ACCTCATGGGTTGTGACTGTATCC | TAGGATCACTGACAGGCATAGCCA |
oprD | TTTCCGCAGGTAGCACTCAGT | CTTCGCTTCGGCCTGATC |
lasA | TTCTGTGATCGATTCGGCTCGGTT | ACCCGGGAAGACAACTATCAGCTT |
lecA | CACCATTGTGTTTCCTGGCGTTCA | AGAAGGCAACGTCGACTCGTTGAT |
lecB | AGACAGCGTAACAATCGAACGAGC | AGGACGCATCGTTCAGCCAATCTA |
rpoS | CGGCGAGTTGGTCATCATCAAACA | ATCGATTGCCCTACCTTGACCTGT |
prtR | TCCCTGCACCCATGTGAAATCTCT | ATCGGCAATCTACAGACCGATGGA |
creD | CGCCATCGCCCTACTCAT | GGCGATCGCGGATCAG |
ampG | GGCCTGACCTATGCGTTGTT | CGACGGGTTGACGTGGAT |
ampP | ACCGCCGCCTGGTCAT | CAGGCCGATGGGAATGC |
creB | GCATATCCTGATCGTCGAAGATG | GGCCTGCAGGGCGTAGA |
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Molina Bertrán, S.d.C.; Monzote, L.; Cappoen, D.; Escalona Arranz, J.C.; Gordillo Pérez, M.J.; Rodríguez-Ferreiro, A.O.; Chill Nuñez, I.; Novo, C.P.; Méndez, D.; Cos, P.; et al. Inhibition of Bacterial Adhesion and Biofilm Formation by Seed-Derived Ethanol Extracts from Persea americana Mill. Molecules 2022, 27, 5009. https://doi.org/10.3390/molecules27155009
Molina Bertrán SdC, Monzote L, Cappoen D, Escalona Arranz JC, Gordillo Pérez MJ, Rodríguez-Ferreiro AO, Chill Nuñez I, Novo CP, Méndez D, Cos P, et al. Inhibition of Bacterial Adhesion and Biofilm Formation by Seed-Derived Ethanol Extracts from Persea americana Mill. Molecules. 2022; 27(15):5009. https://doi.org/10.3390/molecules27155009
Chicago/Turabian StyleMolina Bertrán, Silvia del Carmen, Lianet Monzote, Davie Cappoen, Julio Cesar Escalona Arranz, Mario Juan Gordillo Pérez, Annarli O. Rodríguez-Ferreiro, Idelsy Chill Nuñez, Claudina Pérez Novo, Daniel Méndez, Paul Cos, and et al. 2022. "Inhibition of Bacterial Adhesion and Biofilm Formation by Seed-Derived Ethanol Extracts from Persea americana Mill" Molecules 27, no. 15: 5009. https://doi.org/10.3390/molecules27155009